m6A-mediated Cell-cell Communication Controls Planarian Regeneration

Regeneration is the regrowth of damaged tissues or organs, a vital mechanism responding to damages from primitive organisms to higher mammals. Planarian possesses active whole-body regenerative capability owning to its vast reservoir of adult stem cells, neoblasts, thus provides an ideal model to delineate the underlying mechanisms for regeneration. N 6 -methyladenosine (m 6 A) regulates stem cell renewal and differentiation. However, how m 6 A controls regeneration at whole-organism level remains largely unknown. Here, we demonstrate that the depletion of m 6 A methyltransferase regulatory subunit wtap abolishes planarian regeneration, through regulating cell-cell communication and cell cycle. scRNA-Seq analysis unveils that the wtap knockdown induces a unique type of neural progenitor-like cells (NP-like cells), characterized by specific expression of the cell-cell communication ligand grn . Intriguingly, the depletion of m 6 A-modified transcripts grn / cdk9 (or cdk7 ) axis rescues the defective regeneration of planarian without wtap . Overall, our study reveals an indispensable role of m 6 A-dependent cell-cell communication essential for whole-organism regeneration.