In silico Characterization and Epitope Profiling of Differentially Expressed Plasma Membrane Proteins of ‘X’ and ‘Y’ Chromosome Bearing Bovine Sperm

The specific and differentially expressed plasma membrane proteins of bovine sex sorted semen were selected after reviewing previous research publications related to proteomic profile of bovine sexed semen. The in-silico characters such as physicochemical properties, primary, secondary, tertiary structures, membrane topology, sub-cellular localisation, domain analysis, protein interactions and epitope profiling, both linear and discontinuous peptides was done for selected plasma membrane proteins by using various bioinformatics tools and software. The results showed localisation of protein along with their structure, polarity, stability, domains, their function and probable epitopes of various selected X and Y bovine sperm plasma membrane specific and differentially expressed proteins. For X specific protein CLRN3, 6 and 4 linear and discontinuous epitopes, and for Y chromosome specific protein, SCAMP1, 6 and 8 linear and discontinuous epitopes were predicted, respectively. The results of in silico characterization and epitope prediction of various selected X and Y bovine sperm plasma membrane specific and differentially expressed proteins would be helpful in selection of some efficient antigenic proteins to be used for further X and Y sperm specific antibody production or synthesis of antibodies artificially.