Construction of Biologically Active Recellularized Tissue Engineered Skin to Promote Wound Healing

The application of biomaterial scaffolds are believed to hold tremendous potential for tissue regeneration. Especially extracellular matrix scaffolds though decellularization provide a dramatic approach to overcome the immunogenicity, simulate in-vivo microenvironment, and implement biochemicalor mechanical properties similar to native organs/tissues. On this basis, we employed a decellularization method to develop an acellular porcine skin matrix (APSM) and applied matrisome analysis to compare APSM with BME and natural skin. The results showed that the structural collagen of APSM was fully preserved and the proteoglycans were effectively preserved. Furthermore, we investigated the impact of APSM powders on the proliferation of epidermal cells in vitro. Meanwhile, we successfully reconstructed tissue engineered skin (TE-Skin) using APSM as a carrier scaffold and simultaneously proceeded biomechanical testing on it. Finally, through mouse skin injured model transplantation study, we verified that TE-Skin could promote wound healing.