Microbiota analysis of perimenopausal women experiencing recurrent vaginitis in conjunction with urinary tract infection

Microbiota analysis of perimenopausal women experiencing recurrent vaginitis in conjunction with urinary tract infection | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Microbiota analysis of perimenopausal women experiencing recurrent vaginitis in conjunction with urinary tract infection Yingying Bi, Yuezhu Wang, Wu Li, Yuhang Chen, Jinlong Qin, Huajun Zheng This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4782067/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 04 Jan, 2025 Read the published version in BMC Microbiology → Version 1 posted 15 You are reading this latest preprint version Abstract Background Recurrent vaginitis in conjunction with urinary tract infection (RV/UTI) in perimenopausal women is a common clinical condition that plagues doctors and patients. Its pathogenesis is not completely known, but the urogenital microbiota is thought to be involved. We compared the urogenital and gut microbiota of perimenopausal women experiencing RV/UTI with those of age-matched controls in an attempt to provide a new microbiological perspective and scheme for solving clinical problems. Results Fifty women of perimenopausal age who were diagnosed with RV/UTI and 50 age-matched healthy controls were enrolled. The urogenital and intestinal microbiota were analyzed using 16S ribosomal RNA gene sequencing by collecting samples from the mouth, anus, urine, cervix, and upper and lower vaginal ends.In the microbiota of healthy perimenopausal women, the mouth site showed the highest richness, while the anus and mouth had the highest diversity. Compared with those in healthy controls, in the microbiota of patients with RV/UTI, the evenness of the upper vaginal ends, anus and cervix significantly increased, while the richness and diversity of the cervix significantly decreased. Lactobacillus accounted for 40.65% of the bacteria in the upper vaginal ends and 39.85% of the bacteria in the lower vaginal ends of healthy women of perimenopausal age, and there were no significant differences in Lactobacillus abundance among the patients with RV/UTI. The relative abundance of 54 genera and 97 species showed significant difference between patients and healthy samples, particularly in the cervix and urine. A total of 147 predicted pathways showed significant differences between patients and healthy controls, with the microbiota of the anus exhibited the greatest number of functional changes, followed by that of the urine microbiota. A random forest model composed of eight genera in the upper vaginal ends showed the highest discriminatory power (AUC 99.38%) to predict RV/UTI. Conclusions Our study provides insight into the nature of the urogenital and intestinal microbiota in perimenopausal women, and revealed significant changes of microbiota in the patients with RV/UTI. This will help characterize the relationship between the urogenital microbiota and RV/UTI, potentially aiding in diagnostic and therapeutic strategy development. recurrent vaginitis urinary tract infection urogenital microbiota 16S rRNA gene sequencing Full Text Additional Declarations No competing interests reported. Supplementary Files TableS1.xlsx Additional file 1: Supplementary Table S1. Major genus in difference groups. TableS2.xlsx Additional file 2: Supplementary Table S2. Major species in difference groups. TableS3.xlsx Additional file 3: Supplementary Table S3. The biomarkers in difference sites between patient and healthy controls. TableS4.xlsx Additional file 4: Supplementary Table S4. The microbiome function changed in difference sites between patient and healthy controls. TableS5.xlsx Additional file 5: Supplementary Table S5. The correaltions of species and functional changes. Cite Share Download PDF Status: Published Journal Publication published 04 Jan, 2025 Read the published version in BMC Microbiology → Version 1 posted Editorial decision: Revision requested 23 Sep, 2024 Reviews received at journal 19 Sep, 2024 Reviews received at journal 12 Sep, 2024 Reviews received at journal 05 Sep, 2024 Reviewers agreed at journal 03 Sep, 2024 Reviewers agreed at journal 29 Aug, 2024 Reviewers agreed at journal 28 Aug, 2024 Reviews received at journal 28 Aug, 2024 Reviewers agreed at journal 28 Aug, 2024 Reviewers agreed at journal 27 Aug, 2024 Reviewers invited by journal 22 Aug, 2024 Editor invited by journal 20 Aug, 2024 Editor assigned by journal 13 Aug, 2024 Submission checks completed at journal 13 Aug, 2024 First submitted to journal 22 Jul, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4782067","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":350979708,"identity":"c88ca64a-6d62-401b-9042-d51df7bc8a93","order_by":0,"name":"Yingying Bi","email":"","orcid":"","institution":"Department of Gynaecology, Shanghai Fourth People's Hospital","correspondingAuthor":false,"prefix":"","firstName":"Yingying","middleName":"","lastName":"Bi","suffix":""},{"id":350979709,"identity":"7994d254-4ed8-4712-a229-b07e39e0c9ba","order_by":1,"name":"Yuezhu Wang","email":"","orcid":"","institution":"Shanghai-MOST Key Laboratory of 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Its pathogenesis is not completely known, but the urogenital microbiota is thought to be involved. We compared the urogenital and gut microbiota of perimenopausal women experiencing RV/UTI with those of age-matched controls in an attempt to provide a new microbiological perspective and scheme for solving clinical problems.\u003c/p\u003e\u003ch2\u003eResults\u003c/h2\u003e \u003cp\u003eFifty women of perimenopausal age who were diagnosed with RV/UTI and 50 age-matched healthy controls were enrolled. The urogenital and intestinal microbiota were analyzed using 16S ribosomal RNA gene sequencing by collecting samples from the mouth, anus, urine, cervix, and upper and lower vaginal ends.In the microbiota of healthy perimenopausal women, the mouth site showed the highest richness, while the anus and mouth had the highest diversity. Compared with those in healthy controls, in the microbiota of patients with RV/UTI, the evenness of the upper vaginal ends, anus and cervix significantly increased, while the richness and diversity of the cervix significantly decreased. Lactobacillus accounted for 40.65% of the bacteria in the upper vaginal ends and 39.85% of the bacteria in the lower vaginal ends of healthy women of perimenopausal age, and there were no significant differences in Lactobacillus abundance among the patients with RV/UTI. The relative abundance of 54 genera and 97 species showed significant difference between patients and healthy samples, particularly in the cervix and urine. A total of 147 predicted pathways showed significant differences between patients and healthy controls, with the microbiota of the anus exhibited the greatest number of functional changes, followed by that of the urine microbiota. A random forest model composed of eight genera in the upper vaginal ends showed the highest discriminatory power (AUC 99.38%) to predict RV/UTI.\u003c/p\u003e\u003ch2\u003eConclusions\u003c/h2\u003e \u003cp\u003eOur study provides insight into the nature of the urogenital and intestinal microbiota in perimenopausal women, and revealed significant changes of microbiota in the patients with RV/UTI. This will help characterize the relationship between the urogenital microbiota and RV/UTI, potentially aiding in diagnostic and therapeutic strategy development.\u003c/p\u003e","manuscriptTitle":"Microbiota analysis of perimenopausal women experiencing recurrent vaginitis in conjunction with urinary tract infection","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-09-10 16:12:12","doi":"10.21203/rs.3.rs-4782067/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2024-09-23T08:07:48+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2024-09-19T21:08:43+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2024-09-12T18:59:19+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2024-09-05T11:54:07+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"68439566712543457188524731028966454285","date":"2024-09-03T08:31:08+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"243352145363461407557814216231109558023","date":"2024-08-29T14:21:43+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"113538023265111661543281787836076757968","date":"2024-08-28T17:53:05+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2024-08-28T15:55:56+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"92155211577869244329170500993797591346","date":"2024-08-28T08:28:01+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"184616515414685501091017090156442961043","date":"2024-08-27T05:49:40+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2024-08-22T21:27:41+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2024-08-20T23:00:29+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2024-08-13T16:04:49+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2024-08-13T16:04:06+00:00","index":"","fulltext":""},{"type":"submitted","content":"BMC Microbiology","date":"2024-07-22T12:59:16+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"bmc-microbiology","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"mcro","sideBox":"Learn more about [BMC Microbiology](http://bmcmicrobiol.biomedcentral.com/)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/mcro","title":"BMC Microbiology","twitterHandle":"#bmcmicrobiology","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"em","reportingPortfolio":"BMC Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"e0e0583a-9053-46ba-8b4f-f02da51f3843","owner":[],"postedDate":"September 10th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[],"tags":[],"updatedAt":"2025-01-06T16:02:24+00:00","versionOfRecord":{"articleIdentity":"rs-4782067","link":"https://doi.org/10.1186/s12866-024-03709-3","journal":{"identity":"bmc-microbiology","isVorOnly":false,"title":"BMC Microbiology"},"publishedOn":"2025-01-04 15:57:35","publishedOnDateReadable":"January 4th, 2025"},"versionCreatedAt":"2024-09-10 16:12:12","video":"","vorDoi":"10.1186/s12866-024-03709-3","vorDoiUrl":"https://doi.org/10.1186/s12866-024-03709-3","workflowStages":[]},"version":"v1","identity":"rs-4782067","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4782067","identity":"rs-4782067","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}