MGI short-read genome assemblies of Pythium insidiosum strains Pi057C3 and Pi050C3

Objectives: Pythium insidiosum causes a difficult-to-treat infectious condition called pythiosis, which comes with high morbidity and mortality. So far, genome data of at least 10 P. insidiosum strains, primarily classified in the phylogenetic clades I and II, have been sequenced using various next-generation sequencing platforms. Here, the MGI short-read platform was employed to obtain genome data of 2 clade-III strains of P. insidiosum from patients in Thailand and the United States. This work is a part of our attempt to generate a comprehensive genome database from diverse strains of P. insidiosum . Data description: A 150-bp paired-end library was prepared from a gDNA sample of P. insidiosum strains Pi057C3 and Pi050C3 (also known as ATCC90586) to generate draft genome sequences using an MGISEQ-2000RS sequencer. As a result, for the strain Pi057C3, we obtained a 42.5-Mb assembled genome (164x coverage) comprising 14,134 contigs, N50 of 241, L50 of 45,748, 57.6% CG content, and 12,147 ORFs. For the strain Pi050C3, we received a 43.3-Mb draft genome (230x coverage) containing 14,511 contigs, N50 of 245, L50 of 45,208, 57.7% CG content, and 12,249 ORFs. The genome sequences have been deposited in the NCBI/DDBJ databases under the accession numbers JAKCXM000000000.1 (strain Pi057C3) and JAKCXL000000000.1 (strain Pi050C3).