Integrated Bioinformatic Analysis Reveals NOS2 as a Novel Ferroptosis-related Biomarker for Pre-eclampsia

AbstractBackground Pre-eclampsia (PE) is a common condition in pregnancy; however, methods for early diagnosis and effective treatment options are lacking. Ferroptosis is a newly identified iron-dependent cell death pathway. The aim of this study was to investigate the role of ferroptosis-related genes in PE, the underlying mechanism, and their potential diagnostic value using a bioinformatics approach. Methods We downloaded the GSE48424 and GSE98224 datasets from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) between PE and healthy pregnancy samples were identified in the GSE48424 dataset and subjected to weighted gene co-expression network analysis; the most relevant modules were intersected with known ferroptosis-related genes to distinctly identify the role of ferroptosis in PE. We further searched transcription factors and microRNAs that are predicted to regulate these ferroptosis-related genes, and patients in the GSE48424 dataset were divided into two groups according to high or low expression of the key ferroptosis-related genes associated with PE. To obtain robust key ferroptosis-related genes in PE, we validated their expression levels in the external dataset GSE98224. Finally, we performed a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assay of these genes to evaluate their expression in the placenta samples of patients with PE and normal pregnancy. Results The most relevant module of PE in the GSE48424 dataset comprising the 565 identified DEGs contained a total of 3661 genes. After overlapping, we obtained six ferroptosis-related genes involved in PE. Among these genes, patients with PE displaying lower expression levels ofNOS2and higher expression levels ofPTGS2had a higher ferroptosis potential index. The expression pattern ofNOS2was consistent in the GSE48424 and GSE98224 datasets. RT-qPCR data confirmed thatNOS2expression was more significantly elevated in patients with PE than in those with a normal pregnancy. Conclusions Our study explored the diagnostic value of ferroptosis-related genes in PE, and identifiedNOS2as the key gene linking ferroptosis and PE, suggesting a new candidate biomarker for early PE diagnosis.