Characterization and identification of rice genotypes based on the microsatellite SSR marker for varietal purity assessment

Microsatellite markers prove highly valuable for both assessing genetic purity and diversity. Moreover, efforts have been made to identify an alternative to the Grow-Out-Test, a traditional but laborious and time-consuming method used for seed genetic purity testing in the history of quality seed multiplication. The primary objective of the present study was to examine genetic purity and diversity among ten distinct rice varieties recently released from TNAU i.e., ADT-53, ADT-54, ADT-55, ADT-57, MDU-6, TKM-15, CO-52, CO-55, TRY-5, VGD-1.The results of the experiments revealed that, out of the 50 SSR primers examined, 28 were identified as polymorphic and were subsequently employed in the analysis of these ten rice varieties. Among the primer pairs used for molecular profiling, namely RM 60, RM 3345, RM 5463, RM 205, and RM 512, RM 60, RM 3345, RM 5463, RM 205, and RM 512 emerged as particularly effective in revealing discernible and predictable patterns of polymorphism for genetic purity testing. Major allele frequencies varied from 0.18 (RM 3345) to 0.66 (RM 520), averaging at 0.42. A dendrogram, constructed through cluster analysis based on microsatellite polymorphism, showcased the relationships among the ten rice varieties. These findings have practical implications for the monitoring of purity, genotype identification and the protection of plant varieties.