Effects of an Emotional Release Intervention Studied by Hekalogy on Serum Serotonin Concentrations: A Prospective Pre-Post Study

Effects of an Emotional Release Intervention Studied by Hekalogy on Serum Serotonin Concentrations: A Prospective Pre-Post Study | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Effects of an Emotional Release Intervention Studied by Hekalogy on Serum Serotonin Concentrations: A Prospective Pre-Post Study Jonathan Calderon Nava, Ricardo Ponce Herrera This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8643135/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Serotonin (5-HT) is a critical neuromodulator for affective homeostasis, and alterations in its levels are linked to the pathophysiology of neuropsychiatric disorders. Given the limited evidence on conscious emotional release interventions, this study evaluated the Hekalogy model as an inducer of psychophysiological balance measurable through peripheral biomarkers. This explanatory study used a pre-experimental design with 30 volunteers attending the "Chakra 3" event (Medellín, Colombia). Serum serotonin was quantified by high-performance liquid chromatography (HPLC) under strict fasting conditions, with baseline (T0) and 24-hour post-intervention (T1) measurements. Statistical analysis included the Shapiro-Wilk normality test and the paired Student's t-test with a 95% confidence level. A statistically significant increase in mean serotonin was observed, rising from 105.66 ng/mL to 113.06 ng/mL (p < 0.001), representing a 7% systemic increase. Subjects with reduced baseline levels showed a 34% increase in the lower threshold. Regression analysis indicated that the intervention explained 90.4% of the observed positive variance (R² = 0.904). The Hekalogy-based intervention acts as a biological model that optimizes the peripheral serotonergic system and suggests regulation of the hypothalamic-pituitary-adrenal (HPA) axis. The results validate emotional management as a clinical tool in contemporary integrative medicine. Biological sciences/Psychology/Human behaviour Humanities/Medical humanities Neurobiology Emotional regulation Serotonin Full Text Additional Declarations There is NO Competing Interest. Supplementary Files comitedebioetica.pdf ethics committee Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8643135","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":577471328,"identity":"aed9cca0-d97d-4d81-8826-64ec011c3820","order_by":0,"name":"Jonathan Calderon Nava","email":"data:image/png;base64,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","orcid":"","institution":"HUMAN TRANSCENDENCE","correspondingAuthor":true,"prefix":"","firstName":"Jonathan","middleName":"Calderon","lastName":"Nava","suffix":""},{"id":577471329,"identity":"2723e40d-363e-4e8d-bd74-ac024f013a79","order_by":1,"name":"Ricardo Ponce Herrera","email":"","orcid":"","institution":"HUMAN TRANSCENDENCE","correspondingAuthor":false,"prefix":"","firstName":"Ricardo","middleName":"Ponce","lastName":"Herrera","suffix":""}],"badges":[],"createdAt":"2026-01-19 21:45:11","currentVersionCode":1,"declarations":{"humanSubjects":false,"vertebrateSubjects":false,"conflictsOfInterestStatement":false,"humanSubjectEthicalGuidelines":false,"humanSubjectConsent":false,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":false},"doi":"10.21203/rs.3.rs-8643135/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-8643135/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":100755462,"identity":"fe3c0946-1295-4c9b-89e4-bfdb8132f443","added_by":"auto","created_at":"2026-01-21 06:20:46","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":533296,"visible":true,"origin":"","legend":"Article File","description":"","filename":"Manuscrito.es.en.pdf","url":"https://assets-eu.researchsquare.com/files/rs-8643135/v1_covered_ca0ee46f-0382-4cd0-bd88-da0c677f7b3c.pdf"},{"id":100755443,"identity":"6e91a1ca-211b-4700-add2-e4fdcc5f0323","added_by":"auto","created_at":"2026-01-21 06:20:29","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":206393,"visible":true,"origin":"","legend":"ethics committee","description":"","filename":"comitedebioetica.pdf","url":"https://assets-eu.researchsquare.com/files/rs-8643135/v1/0490bea663f7a654d9490565.pdf"}],"financialInterests":"There is \u003cb\u003eNO\u003c/b\u003e Competing Interest.","formattedTitle":"\u003cp\u003eEffects of an Emotional Release Intervention Studied by Hekalogy on Serum Serotonin Concentrations: A Prospective Pre-Post Study\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":true,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Neurobiology, Emotional regulation, Serotonin","lastPublishedDoi":"10.21203/rs.3.rs-8643135/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-8643135/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Serotonin (5-HT) is a critical neuromodulator for affective homeostasis, and alterations in its levels are linked to the pathophysiology of neuropsychiatric disorders. Given the limited evidence on conscious emotional release interventions, this study evaluated the Hekalogy model as an inducer of psychophysiological balance measurable through peripheral biomarkers. This explanatory study used a pre-experimental design with 30 volunteers attending the \"Chakra 3\" event (Medellín, Colombia). Serum serotonin was quantified by high-performance liquid chromatography (HPLC) under strict fasting conditions, with baseline (T0) and 24-hour post-intervention (T1) measurements. Statistical analysis included the Shapiro-Wilk normality test and the paired Student's t-test with a 95% confidence level. A statistically significant increase in mean serotonin was observed, rising from 105.66 ng/mL to 113.06 ng/mL (p \u003c 0.001), representing a 7% systemic increase. Subjects with reduced baseline levels showed a 34% increase in the lower threshold. Regression analysis indicated that the intervention explained 90.4% of the observed positive variance (R² = 0.904). The Hekalogy-based intervention acts as a biological model that optimizes the peripheral serotonergic system and suggests regulation of the hypothalamic-pituitary-adrenal (HPA) axis. 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