Why didn't the quail cross the road? Using whole-genome data to evaluate roadways as barriers to gene flow in a North American ground-dwelling bird

Why didn't the quail cross the road? Using whole-genome data to evaluate roadways as barriers to gene flow in a North American ground-dwelling bird | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 5 April 2025 V1 Latest version Share on Why didn't the quail cross the road? Using whole-genome data to evaluate roadways as barriers to gene flow in a North American ground-dwelling bird Authors : Joseph Curti 0000-0003-4630-9073 [email protected] , Zachary MacDonald 0000-0002-7966-5712 , Phred Benham 0000-0003-3276-6177 , Rauri Bowie , Katy Delaney , Ryan Harrigan , Kirk Lohmueller , Seth Riley , Allison Shultz 0000-0002-2089-4086 , Robert Wayne 0000-0003-3537-2245 , and H. Bradley Shaffer 0000-0002-5795-9242 Authors Info & Affiliations https://doi.org/10.22541/au.174387447.73877055/v1 648 views 214 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Roadway infrastructure is a dominating feature of landscapes across the globe and the ecological and evolutionary impacts of these roadways are leading to declines in many natural populations. To date, research on the genetic impacts of roadways on bird populations has produced mixed results, with some studies revealing significant correlations between roads and genetic differentiation and others failing to resolve a significant relationship. Here, we generate a whole-genome dataset to quantify the impacts of roadway infrastructure on the population genetic differentiation of California quail (Callipepla californica), a ground-dwelling and ground-nesting bird species. We non-lethally sampled wild quail from populations located on either side of major and minor roadways in Southern California, a region with the densest road network in the United States. Using a variety of landscape genetic and statistical models, we demonstrate that roadways are the most important factor shaping patterns of genomic differentiation in southern California quail populations. We also show that quail populations sampled alongside the two busiest roadways traversing California have higher levels of inbreeding than other populations within the region, likely resulting from close kin matings within the last 10 generations. Results of this study highlight the importance of roads as major disturbances to genetic connectivity of wild populations and can help inform regional connectivity conservation efforts, including the construction of the Wallis Annenberg Wildlife Crossing over the US 101 freeway. Supplementary Material File (me_manuscript (1).docx) Download 3.01 MB Information & Authors Information Version history V1 Version 1 05 April 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords callipepla californica conservation galliformes isolation by resistance landscape genomics road ecology Authors Affiliations Joseph Curti 0000-0003-4630-9073 [email protected] University of California Los Angeles View all articles by this author Zachary MacDonald 0000-0002-7966-5712 University of California Los Angeles View all articles by this author Phred Benham 0000-0003-3276-6177 University of California Berkeley View all articles by this author Rauri Bowie University of California Berkeley View all articles by this author Katy Delaney National Park Service View all articles by this author Ryan Harrigan University of California Los Angeles View all articles by this author Kirk Lohmueller University of California Los Angeles View all articles by this author Seth Riley National Park Service View all articles by this author Allison Shultz 0000-0002-2089-4086 Natural History Museum of Los Angeles County View all articles by this author Robert Wayne 0000-0003-3537-2245 University of California Los Angeles View all articles by this author H. Bradley Shaffer 0000-0002-5795-9242 University of California Los Angeles View all articles by this author Metrics & Citations Metrics Article Usage 648 views 214 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Joseph Curti, Zachary MacDonald, Phred Benham, et al. Why didn't the quail cross the road? Using whole-genome data to evaluate roadways as barriers to gene flow in a North American ground-dwelling bird. Authorea . 05 April 2025. DOI: https://doi.org/10.22541/au.174387447.73877055/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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