Immunomodulatory Multifunctional Janus Collagen-based Membrane for Advanced Bone Regeneration

Immunomodulatory Multifunctional Janus Collagen-based Membrane for Advanced Bone Regeneration | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Immunomodulatory Multifunctional Janus Collagen-based Membrane for Advanced Bone Regeneration Defu Li, Die Yang, Zhilang Xu, Dou Huang, Qi Luo, Chunli Zhang, and 4 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5074288/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 08 May, 2025 Read the published version in Nature Communications → Version 1 posted You are reading this latest preprint version Abstract Guided bone regeneration (GBR) is a standard therapy for treating bone defects, with collagen-based barrier membranes widely used clinically. However, these membranes face challenges like early bacterial invasion, risking GBR failure. Effective strategies include orchestrating macrophage activation and controlling their M1 or M2 polarization during bone repair. We present a novel Janus collagen-based barrier membrane with immunomodulatory function. The porous layer promotes direct osteogenic differentiation and inward growth of osteoblasts. The dense layer prevents invasion of soft tissue into bone defects and protects bone defects from bacterial infection. The membrane also enhances rat bone marrow-derived mesenchymal stem cells (rBMSCs) infiltration, proliferation, and osteogenic differentiation by regulating the immune microenvironment. Cranial defect experiments show superior bone regeneration compared to the commercially available Bio-Gide® collagen membrane. Overall, the Janus collagen-based membrane reduces tissue inflammation and fosters an osteoimmune environment conducive to new bone formation, offering innovative material design for advanced GBR technology. Biological sciences/Chemical biology/Membranes Biological sciences/Immunology Guided bone regeneration Barrier membrane Antibacterial activity Nano-hydroxyapatite Macrophages Full Text Additional Declarations There is NO Competing Interest. Supplementary Files SupportingInformation.pdf Cite Share Download PDF Status: Published Journal Publication published 08 May, 2025 Read the published version in Nature Communications → Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5074288","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":355359628,"identity":"7ede2256-99f5-4633-bdda-c1ff0248219a","order_by":0,"name":"Defu 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