The lack of a COPII cargo receptor Erv14 impacts physiological functions of the vacuole in Saccharomyces cerevisiae

2025 · doi:10.22541/au.175017818.87254760/v1
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Abstract

Erv14 is a cargo receptor of COPII vesicles, which is necessary for the efficient trafficking of various membrane proteins. In this work, we demonstrate that the deletion of the ERV14 gene impacts various physiological functions of the vacuole. Compared to the wild-type cells, cells lacking the ERV14 gene exhibited higher vacuolar pH and altered vacuolar morphology with increased fragmentation. In addition, erv14Δ cells exhibit thinner cell wall and impaired process of endocytosis. We also found the importance of ERV14 for cells to overcome environmental stresses, such as neutral external pH, increased zinc and calcium concentrations, and high temperature. Furthermore, comparing the gene expression, proteome analysis, and structural modeling revealed new interactions between Erv14 and several vacuolar proteins, including subunits of V-ATPase, and other proteins involved in carbon metabolism.
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Abstract

Erv14 is a cargo receptor of COPII vesicles, which is necessary for the efficient trafficking of various membrane proteins. In this work, we demonstrate that the deletion of the ERV14 gene impacts various physiological functions of the vacuole. Compared to the wild-type cells, cells lacking the ERV14 gene exhibited higher vacuolar pH and altered vacuolar morphology with increased fragmentation. In addition, erv14Δ cells exhibit thinner cell wall and impaired process of endocytosis. We also found the importance of ERV14 for cells to overcome environmental stresses, such as neutral external pH, increased zinc and calcium concentrations, and high temperature. Furthermore, comparing the gene expression, proteome analysis, and structural modeling revealed new interactions between Erv14 and several vacuolar proteins, including subunits of V-ATPase, and other proteins involved in carbon metabolism. Supplementary Material File (rosas-santiago-etal-2025.docx) - Download - 100.96 KB Information & Authors Information Version history Peer review timeline Published Traffic Version of Record23 Apr 2026Published Copyright This work is licensed under a Non Exclusive No Reuse License.

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Authors Metrics & Citations Metrics Article Usage 388views 177downloads Citations Download citation Paul Rosas-Santiago, Jorge Luis Ruiz Salas, Elizabeth Cordoba, et al. The lack of a COPII cargo receptor Erv14 impacts physiological functions of the vacuole in Saccharomyces cerevisiae. Authorea. 17 June 2025. DOI: https://doi.org/10.22541/au.175017818.87254760/v1 DOI: https://doi.org/10.22541/au.175017818.87254760/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu.

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