Electrochemical Detection of Some Water Soluble and Fat Soluble Vitamins by Using Bare Pencil Graphite Electrode

Abstract Vitamins are a group of organic compounds needed in small quantities by the body to perform its essential functions like growing, regulating metabolism and maintain a healty immune system. In recent years, substantial efforts have been focused on development of simplified, fast and cost-effective approaches for vitamin analysis. Aim of this study was electrochemical detection of some water and fat-soluble vitamins individualy and simultaneously by using bare pencil graphite electrode (PGE). Vitamins of C, B1, B6, B12, A, D3, and E were selected and electrochemically analyzed in pharmaceutical preparates. Simultaneous analysis of water-soluble vitamins by using DPV with PGE can be promising technique for the determination of vitamin C and B6 or vitamin B1, B12 and B6. In the case of fat-soluble vitamins, resolution between vitamin D3 and E was not satisfactory. However, vitamin A and D or vitamin A and E could be detected in a single run. Limit of detection of the selected vitamins were quite low (4-548 µg/ml).