Autophagy and the mitochondrial Lon1 protease are necessary for Botrytis cinerea heat adaptation

Autophagy and the mitochondrial Lon1 protease are necessary for Botrytis cinerea heat adaptation | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL Molecular Microbiology This is a preprint and has not been peer reviewed. Data may be preliminary. 27 January 2025 V1 Latest version Share on Autophagy and the mitochondrial Lon1 protease are necessary for Botrytis cinerea heat adaptation Authors : Mingzhe Zhang 0000-0002-4900-581X , Liang Ma , Zhiqun LV , Naomi Kagan Trushina , and Amir Sharon [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.173796694.42647087/v1 Published Molecular Microbiology Version of record Peer review timeline 396 views 197 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Heat adaptation is a multilayered universal process involving a coordinated response of general and heat-specific cellular systems and processes. Here, we demonstrate that adaptation of the plant pathogenic fungus Botrytis cinerea to mild heat stress requires both autophagy and the mitochondrial Lon1 protease. Deleting bclon1 or blocking autophagy by deleting the bcatg1 autophagy-regulating gene did not affect fungal survival at optimal temperature. Under heat stress, deletion of bclon1 induced earlier and more intense autophagy, mitochondrial malfunction, and accelerated fungal cell death. These phenomena were intensified in a bcatg1/lon1 double mutant, indicating coordinated activity of both pathways in heat adaptation. Blocking autophagy, but not bclon1, also affected mycelia growth, spore germination, as well as nuclei division and spore morphology. Our results support a cytoprotective role for autophagy downstream of mitochondria-driven death signals, possibly as a mechanism that promotes growth arrest and helps remove damaged cellular components. Supplementary Material File (manuscript.docx) Download 7.76 MB Information & Authors Information Version history V1 Version 1 27 January 2025 Peer review timeline Published Molecular Microbiology Version of Record 18 Jul 2025 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Collection Molecular Microbiology Authors Affiliations Mingzhe Zhang 0000-0002-4900-581X Tel Aviv University School of Plant Sciences and Food Security View all articles by this author Liang Ma Guangdong Institute of Eco-Environmental and Soil Sciences View all articles by this author Zhiqun LV Tel Aviv University School of Plant Sciences and Food Security View all articles by this author Naomi Kagan Trushina Tel Aviv University School of Plant Sciences and Food Security View all articles by this author Amir Sharon [email protected] Tel Aviv University School of Plant Sciences and Food Security View all articles by this author Metrics & Citations Metrics Article Usage 396 views 197 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Mingzhe Zhang, Liang Ma, Zhiqun LV, et al. Autophagy and the mitochondrial Lon1 protease are necessary for Botrytis cinerea heat adaptation. Authorea . 27 January 2025. 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