Arbuscular Mycorrhizal Fungi Co-Colonizing on A Single Plant Root System Recruit Distinct Microbiomes

Arbuscular Mycorrhizal Fungi Co-Colonizing on A Single Plant Root System Recruit Distinct Microbiomes | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Arbuscular Mycorrhizal Fungi Co-Colonizing on A Single Plant Root System Recruit Distinct Microbiomes Jiachao Zhou, Xiaofen Chai, Lin Zhang, Timothy S George, Gu Feng This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-73473/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background : Plant roots are usually colonized by various arbuscular mycorrhizal (AM) fungal species which vary in morphological, physiological and genetic traits and constitute the mycorrhizal nutrient uptake pathway (MP) in addition to roots. Simultaneously, the extraradical hyphae of each AM fungus is associated with a community of bacteria. However, whether the community structure and function of microbiome on the extraradical hyphae would differ between the AM fungal species are mostly unknown. Methods : In order to understand the community structure and the predicted functions of the microbiome associated with different AM fungal species, a split-root compartmented rhizobox culturing system, which allowed us to inoculate two AM fungal species separately in two root compartments was used. We inoculated two separate AM fungal species combinations, Funneliformis mosseae ( F.m ) and Gigaspora margarita ( G.m ), Rhizophagus intraradices ( R.i ) and G. margarita, on a single root system of cotton . The hyphal exudate fed active microbiome was measured by combining 13 C-DNA stable isotope probing with Miseq sequencing. Results : We found different AM fungal species, that were simultaneously colonizing on a single root system, hosted distinct active microbiomes from one another. Moreover, the predicted potential functions of the different microbiomes were distinct. Conclusion : We conclude that the arbuscular mycorrhizal fungi component of the system is responsible for the recruitment distinct microbiomes in the hyphosphere. The potential significance of the predicted functions of the microbiome ecosystem services is discussed. General Microbiology Arbuscular mycorrhizae 13C-DNA-SIP Hyphosphere Microbiome COG Hyphal exudates Mycorrhizal pathway. Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Full Text Supplementary Files Supplementaryimformation.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-73473","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research","associatedPublications":[],"authors":[{"id":2186738,"identity":"a5301f49-fa07-442e-984e-b6f19694f64c","order_by":0,"name":"Jiachao Zhou","email":"","orcid":"","institution":"China Agricultural University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Jiachao","middleName":"","lastName":"Zhou","suffix":""},{"id":2186739,"identity":"7fbc19be-d510-4a60-84c9-bf9b9c7f256c","order_by":1,"name":"Xiaofen Chai","email":"","orcid":"","institution":"China Agricultural 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Mycorrhizal pathway.","lastPublishedDoi":"10.21203/rs.3.rs-73473/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-73473/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eBackground\u003c/strong\u003e: Plant roots are usually colonized by various arbuscular mycorrhizal (AM) fungal species which vary in morphological, physiological and genetic traits and constitute the mycorrhizal nutrient uptake pathway (MP) in addition to roots. Simultaneously, the extraradical hyphae of each AM fungus is associated with a community of bacteria. However, whether the community structure and function of microbiome on the extraradical hyphae would differ between the AM fungal species are mostly unknown. \u003c/p\u003e\u003cp\u003e\u003cstrong\u003eMethods\u003c/strong\u003e: In order to understand the community structure and the predicted functions of the microbiome associated with different AM fungal species, a split-root compartmented rhizobox culturing system, which allowed us to inoculate two AM fungal species separately in two root compartments was used. We inoculated two separate AM fungal species combinations, Funneliformis mosseae ( F.m ) and Gigaspora margarita ( G.m ), Rhizophagus intraradices ( R.i ) and G. margarita, on a single root system of cotton . The hyphal exudate fed active microbiome was measured by combining 13 C-DNA stable isotope probing with Miseq sequencing. \u003c/p\u003e\u003cp\u003e\u003cstrong\u003eResults\u003c/strong\u003e: We found different AM fungal species, that were simultaneously colonizing on a single root system, hosted distinct active microbiomes from one another. Moreover, the predicted potential functions of the different microbiomes were distinct. \u003c/p\u003e\u003cp\u003e\u003cstrong\u003eConclusion\u003c/strong\u003e: We conclude that the arbuscular mycorrhizal fungi component of the system is responsible for the recruitment distinct microbiomes in the hyphosphere. The potential significance of the predicted functions of the microbiome ecosystem services is discussed.\u003c/p\u003e","manuscriptTitle":"Arbuscular Mycorrhizal Fungi Co-Colonizing on A Single Plant Root System Recruit Distinct Microbiomes","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2020-09-10 11:18:09","doi":"10.21203/rs.3.rs-73473/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"88fcdbde-cdb0-43de-8f41-e3c5ed456dbc","owner":[],"postedDate":"September 10th, 2020","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":478876,"name":"General Microbiology"}],"tags":[],"updatedAt":"2020-09-12T19:45:38+00:00","versionOfRecord":[],"versionCreatedAt":"2020-09-10 11:18:09","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-73473","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-73473","identity":"rs-73473","version":["v1"]},"buildId":"FbvkV6FR0MCFSLy54lSbu","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}