Graphene oxide/Fucoidan/Chitosan reinforced poly(vinyl alcohol) nanocomposites films for tissue engineering applications

In this study, both conductive and biocompatible carbon nano-based graphene oxide (GrO), polysaccharide-containing Fucoidan (Fuc) and Chitosan (Cs) materials are doped to PVA polymer and nanocomposite films (NCFs) are designed by electrspun method, suitable for use in biomedical applications. The characterization of the designed NCFs (TEM, XRD, FTIR, TGA-DSC) and the enhancement of cell attachment were investigated. Scanning electron microscopy (SEM) proved the homogeneous and beadless nature of NCFs. Biocompatibility testing was performed for 1, 3 and 7 days of incubation with the fibroblast cell line and the results showed that addition of Fuc increased the bioactivity of NCFs. In addition, the biocompatibility results showed that Fuc-loaded NCFs had the highest viability for all incubation times compared to the others. In addition, the tensile test results; showed that the maximum tensile strength value was observed for NCFs. The designed NCFs have been adapted to mathematical models with the results of Franz diffusion, showing high conductivity due to GrO supplements, affecting biodegradation times between varying pH values, and franz diffusion kinetics.