The Optimized Jugular Vein Catheterization Reinforced Self-Administration Model for Drug Addiction in Adult Male Sprague-Dawley Rats

Background: The self-administration (SA) model represents one of the most important and classic methods for drug addiction, and jugular vein catheterization is one of the most critical techniques in this animal model. We aimed to explore an optimized scheme to improve the success rate of rat jugular vein catheterization and SA model. New method Our experiment provided an optimized scheme which including numerous details, materials, approaches, updated techniques and protocols, etc. Our experimental group consisted of 120 adult male Sprague-Dawley rats, which were divided into the Traditional Operation group (TO group) and the Optimized Operation group (OO group) by the random number table method and then further individually divided into the Saline Training group and the Cocaine Training group for the following SA training. Results: Our results showed that the success rate of the jugular vein catheterization in the OO group was significantly greater than that in the TO group (93.33% vs 46.67%, χ 2 =31.11, P< 0.001). The optimized jugular vein catheterization could make the SA model more stable, reliable and efficient than the traditional operation. Comparison with existing methods Compared with traditional methods, our optimized scheme made numerous improvements in materials and techniques including uniformity, individualized variability of the S-type positioning nail, the length and connection matching, the shape of the end and low cost, etc. Conclusions: Our optimized scheme could provide a more stable and efficient tool for basic research on drug addiction. Several subtle improvements under our personal experience are usually important for augmenting operational efficiency.