Tn-seq profiling reveals that NodS of the beta-rhizobium Paraburkholderia phymatum is detrimental for nodulating soybean

Tn-seq profiling reveals that NodS of the beta-rhizobium Paraburkholderia phymatum is detrimental for nodulating soybean | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Tn-seq profiling reveals that NodS of the beta-rhizobium Paraburkholderia phymatum is detrimental for nodulating soybean Gabriella Pessi, Paula Bellés-Sancho, Daphné Golaz, Sarah Paszti, and 5 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4046625/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 27 Dec, 2024 Read the published version in Communications Biology → Version 1 posted You are reading this latest preprint version Abstract The beta-rhizobial strain Paraburkholderia phymatum STM815 T is noteworthy for its wide host range in nodulating legumes, primarily mimosoids (over 50 different species) but also some papilionoids. It cannot, however, nodulate soybean ( Glycine max [L.] Merr.), one of the world’s most important crops. Here, we constructed a highly saturated genome-wide transposon library of a P. phymatum strain and employed a transposon sequencing (Tn-seq) approach to investigate the underlying genetic mechanisms of symbiotic incompatibility between P. phymatum and soybean. Soybean seedlings inoculated with the P. phymatum Tn-seq library displayed nodules on the roots that were mainly occupied by different mutants in a gene, nodS , coding for a methyltransferase involved in the biosynthesis of nodulation factors. The construction of a nodS deletion strain and a complemented mutant confirmed that nodS is responsible for the nodulation-incompatibility of P. phymatum with soybean. Moreover, infection tests with different host plants revealed that NodS is necessary for optimal nodulation of common bean ( Phaseolus vulgaris ), but it is not required for nodulation of its natural host Mimosa pudica . In conclusion, our results suggest that NodS is involved in determining nodulation specificity of P. phymatum . Biological sciences/Microbiology/Environmental microbiology/Soil microbiology Biological sciences/Plant sciences/Plant symbiosis/Rhizobial symbiosis beta-rhizobia Tn-seq core genes host-specificity nodulation factors broad-host range Full Text Additional Declarations There is NO Competing Interest. Supplementary Files BellXXsetal2023NodSSupplementaryMaterialv1.pdf Supplementary Material SupplementaryTable3.xlsx Supplementary Table 3 SupplementaryTable2.xlsx Supplementary Table 2 Cite Share Download PDF Status: Published Journal Publication published 27 Dec, 2024 Read the published version in Communications Biology → Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4046625","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":279265623,"identity":"bc0697ab-425e-4568-a8f9-04c196cea633","order_by":0,"name":"Gabriella 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soybean","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"nature-portfolio","isNatureJournal":true,"hasQc":false,"allowDirectSubmit":false,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"","title":"Nature Portfolio","twitterHandle":"","acdcEnabled":false,"dfaEnabled":false,"editorialSystem":"ejp","reportingPortfolio":"","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"beta-rhizobia, Tn-seq, core genes, host-specificity, nodulation factors, broad-host range","lastPublishedDoi":"10.21203/rs.3.rs-4046625/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4046625/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"The beta-rhizobial strain \u003ci\u003eParaburkholderia phymatum\u003c/i\u003e STM815\u003csup\u003eT\u003c/sup\u003e is noteworthy for its wide host range in nodulating legumes, primarily mimosoids (over 50 different species) but also some papilionoids. It cannot, however, nodulate soybean (\u003ci\u003eGlycine max\u003c/i\u003e [L.] Merr.), one of the world’s most important crops. Here, we constructed a highly saturated genome-wide transposon library of a \u003ci\u003eP. phymatum\u003c/i\u003e strain and employed a transposon sequencing (Tn-seq) approach to investigate the underlying genetic mechanisms of symbiotic incompatibility between \u003ci\u003eP. phymatum\u003c/i\u003e and soybean. Soybean seedlings inoculated with the \u003ci\u003eP. phymatum\u003c/i\u003e Tn-seq library displayed nodules on the roots that were mainly occupied by different mutants in a gene, \u003ci\u003enodS\u003c/i\u003e, coding for a methyltransferase involved in the biosynthesis of nodulation factors. The construction of a \u003ci\u003enodS\u003c/i\u003e deletion strain and a complemented mutant confirmed that \u003ci\u003enodS\u003c/i\u003e is responsible for the nodulation-incompatibility of \u003ci\u003eP. phymatum\u003c/i\u003e with soybean. Moreover, infection tests with different host plants revealed that NodS is necessary for optimal nodulation of common bean (\u003ci\u003ePhaseolus vulgaris\u003c/i\u003e), but it is not required for nodulation of its natural host \u003ci\u003eMimosa pudica\u003c/i\u003e. In conclusion, our results suggest that NodS is involved in determining nodulation specificity of \u003ci\u003eP. phymatum\u003c/i\u003e.","manuscriptTitle":"Tn-seq profiling reveals that NodS of the beta-rhizobium Paraburkholderia phymatum is detrimental for nodulating soybean","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-03-28 02:25:41","doi":"10.21203/rs.3.rs-4046625/v1","editorialEvents":[],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"communications-biology","isNatureJournal":true,"hasQc":false,"allowDirectSubmit":false,"externalIdentity":"commsbio","sideBox":"Learn more about [Communications Biology](http://www.nature.com/commsbio/)","snPcode":"","submissionUrl":"","title":"Communications Biology","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"ejp","reportingPortfolio":"Communications Series","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"43e5b674-208a-4f2f-a93a-4a98912fa9e3","owner":[],"postedDate":"March 28th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[{"id":29411475,"name":"Biological sciences/Microbiology/Environmental microbiology/Soil microbiology"},{"id":29411476,"name":"Biological sciences/Plant sciences/Plant symbiosis/Rhizobial symbiosis"}],"tags":[],"updatedAt":"2024-12-28T08:07:39+00:00","versionOfRecord":{"articleIdentity":"rs-4046625","link":"https://doi.org/10.1038/s42003-024-07385-x","journal":{"identity":"communications-biology","isVorOnly":false,"title":"Communications Biology"},"publishedOn":"2024-12-27 05:00:00","publishedOnDateReadable":"December 27th, 2024"},"versionCreatedAt":"2024-03-28 02:25:41","video":"","vorDoi":"10.1038/s42003-024-07385-x","vorDoiUrl":"https://doi.org/10.1038/s42003-024-07385-x","workflowStages":[]},"version":"v1","identity":"rs-4046625","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4046625","identity":"rs-4046625","version":["v1"]},"buildId":"WrCJVZZCHTDjtuVLN7oU0","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}