Malignant transformation of endometriosis: application of laser microdissection for analysis of genetic alterations according to pathological changes

Akihiko Sekizawa, Sekizawa A, Amemiya S, Satoshi Amemiya, Otsuka J, Junko Otsuka, Hiroshi Saito, Saito H, Antonio Farina, Farina A, Okai T, Takashi Okai, Tetsuhiko Tachikawa, Tachikawa T
Medical electron microscopy : official journal of the Clinical Electron Microscopy Society of Japan · 2004 · doi:10.1007/s00795-003-0233-0 · PMID:15221651
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Laser microdissection identified K-ras mutations associated with the malignant transformation of endometriosis to clear cell carcinoma via atypical endometriosis.

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The paper examined malignant transformation of ovarian endometriosis, describing a pathological progression from endometriosis to atypical endometriosis and then to clear cell carcinoma or endometriotic carcinoma. Using laser microdissection, the authors collected tissue from regions of normal endometriosis, atypical endometriosis, and clear cell carcinoma and assessed K-ras mutations across these pathological stages. They found K-ras mutations associated with malignant transformation in clear cell carcinoma. The study’s limitation is that the genetic analysis focuses specifically on K-ras in clear cell carcinoma rather than providing a broader, multi-gene assessment across all transformation stages. This paper is centrally about endometriosis — it directly investigates the malignant transformation of endometriosis to clear cell carcinoma using laser microdissection and genetic alteration analysis.

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Abstract

Endometriosis is considered to be a possible precancerous disease. Pathologically, we observed malignant transformation of endometriosis to clear cell carcinoma or endometriotic carcinoma of the ovary, via the step of atypical endometriosis. Pathological changes reflected genetic alterations. In cases of clear cell carcinoma, we assessed K- ras mutations in regions of normal endometriosis, atypical endometriosis, and clear cell carcinoma, and obtained tissue samples by laser microdissection. We found that K- ras mutations were associated with malignant transformation of clear cell carcinoma. The present results indicate that laser microdissection can be used to combine assessment of genetic and pathological changes.
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Abstract Endometriosis is considered to be a possible precancerous disease. Pathologically, we observed malignant transformation of endometriosis to clear cell carcinoma or endometriotic carcinoma of the ovary, via the step of atypical endometriosis. Pathological changes reflected genetic alterations. In cases of clear cell carcinoma, we assessed K-ras mutations in regions of normal endometriosis, atypical endometriosis, and clear cell carcinoma, and obtained tissue samples by laser microdissection. We found that K-ras mutations were associated with malignant transformation of clear cell carcinoma. The present results indicate that laser microdissection can be used to combine assessment of genetic and pathological changes. Similar content being viewed by others Author information Authors and Affiliations Corresponding author Rights and permissions About this article Cite this article Sekizawa, A., Amemiya, S., Otsuka, J. et al. Malignant transformation of endometriosis: application of laser microdissection for analysis of genetic alterations according to pathological changes. Med Electron Microsc 37, 97–100 (2004). https://doi.org/10.1007/s00795-003-0233-0 Received: Accepted: Issue date: DOI: https://doi.org/10.1007/s00795-003-0233-0

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Condition tags

endometriosis

MeSH descriptors

Adenocarcinoma, Clear Cell Endometriosis Genes, ras Lasers Mutation Ovarian Neoplasms Adenocarcinoma, Clear Cell Adenocarcinoma, Clear Cell Cell Transformation, Neoplastic Dissection Endometriosis Endometriosis Female Humans Ovarian Neoplasms Ovarian Neoplasms

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