Salvia Miltiorrhiza Polysaccharides Alleviates Florfenicol-Induced Kidney Injury Via Modulation of Nuclear Factor Kappa-B (NF-κB) Signaling Pathway In Broilers

Abstract Kidney, as an important excretory organ in the body, is easily affected by various pathogenic factors. This research aimed to explore the mechanism of mitigative role of salvia miltiorrhiza polysaccharides（SMPs）on Florfenicol-caused renal injury in chickens. Kidney histological study and uric acid（UA）、creatinine (Cr)、urea nitrogen (BUN) contents in serum was performed to assess the effects of FFC and SMPs on kidneys. The expression of key factors in NF-κB pathway were detected by RT-PCR and Western blot. We also used ELISA to detect the expression of inflammatory cytokines TNF-α, IL-1β and IL-6 in the kidney. The results showed that SMPs could reduce the levels of UA, Cr, BUN and renal pathology of FFC-induced kidney injury, and reduce the expressions of TNF-α, IL-1β and IL-6 in the kidney of broilers (P<0.05). 5.00 g/L SMPs inhibited the expression of NF-κBp65 and IκKβ in renal tissue, and the expression of IκBα was increased (P<0.05). Conclusion: SMPs treatment can protect kidney injury of broilers by inhibiting NF-κB signaling pathway.