Polyene Phosphatidyl Choline Injection Regulates Lipid Homeostasis via AKT-PDE3-PKA in Mice

Objective: This study aimed to the clinical efficacy of polyene phosphatidyl choline (PPC) injections was assessed using data from the Nanjing Hospital of Chinese Medicine. Then investigate the mechanisms of PPC injections in the prevention and treatment of alcoholic liver disease (ALD) in mice. Methods: First, clinical data of PPC injections from the Chinese Medicine Modernization and Big Data Research Center at Nanjing Hospital of Chinese Medicine were collected and analyzed to assess the clinical effects of PPC injections. Then, a mouse model of alcoholic liver injury was established using C57BL/6J mice. The protective effects of PPC injections against ALD were evaluated at the systemic level using biochemical and histopathological analyses. RNA-seq technology was used to identify specific differentially expressed genes and related signaling pathways involved in the protective effects of PPC injections against alcoholic liver injury. Finally, Changes in differentially expressed genes and related signaling pathways were confirmed by western blot analysis of the liver tissues. Results: Clinical data showed that PPC injection has a significant regulatory effect on abnormal liver damage and lipid metabolism-related indicators. PPC injection significantly inhibited the inflammatory response, oxidative stress, and hepatic lipid accumulation in a mouse model of liver injury. The PPC injection can downregulate Akt1, Traf3, Prkaca, NF-KB; and upregulate Pde3b expression. Conclusion: PPC injections had clinically significant hepatoprotective effects. The underlying mechanism may involve may exert its effects on preventing and treating ALD by regulating the Akt-PDE3-PKA signaling pathway to modulate lipid homeostasis.