Evaluating different methods for kidney recellularization

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Luque-Badillo, Cesar U. Monjaras-Avila, Hans Adomat, Alan So, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3943939/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 09 Oct, 2024 Read the published version in Scientific Reports → Version 1 posted 10 You are reading this latest preprint version Abstract This study explores a potential solution to the shortage of kidneys for transplantation in end-stage renal disease (ESRD). Currently, kidney transplantation is the best option, but there aren't enough organs available. The researchers used tissue engineering to evaluate the possibility for creating kidneys for transplantation. They used pig kidneys, removing cells through a process called decellularization while preserving the organ's microarchitecture. The researchers then perfused pig kidney cells and human red blood cells to the empty kidney structure to recreate it. The methodologies used, showed promising results, suggesting a hopeful method to address the organ shortage. This proof-of-concept lays the foundation for potentially applying this technology to human kidneys, which could significantly improve outcomes for ESRD patients by providing more transplantable organs. Health sciences/Medical research Health sciences/Urology Physical sciences/Engineering Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Introduction End-stage renal disease (ESRD) is a condition in which the kidneys lose their ability to filter toxins and excess fluids from the bloodstream, leading to a dangerous accumulation of waste products and electrolytes in the body. This not only reduces the patient's quality of life but also results in premature mortality 1. In 2021, the prevalence of ESRD in Canada reached 48,000 cases, marking a 24% increase over the past decade. This surge in cases places a significant annual healthcare burden of 40 billion dollars on the system 2–4. The prevalence of ESRD is expected to rise further due to its strong association with chronic diseases like diabetes mellitus and hypertension, both of which are prevalent in the aging Canadian population 5–7. There are two primary treatments for ESRD: dialysis and kidney transplant 4. Although kidney transplant is considered the optimal treatment, most patients must undergo dialysis initially. The demand for chronic dialysis has nearly doubled over the past two decades, costing the Canadian healthcare system over $ 100,000 per patient annually 3,8. Kidney transplant remains the most cost-effective and survival-enhancing option, with a 41% increase in transplant cases in the last decade 10 . However, the shortage of available organs is a critical issue, with 77% of individuals on the organ waitlist in Canada awaiting a kidney, with an average wait time of 3 years and ten months 4,10,11. To address this organ shortage problem, tissue engineering emerges as a potential solution. Tissue engineering combines engineering principles with cell biology to develop biological substitutes capable of restoring or enhancing tissue and organ function. One technique in tissue engineering is decellularization, which removes cellular components from tissues or organs while preserving the extracellular matrix (ECM). The ECM provides structural support, native vascular networks, and bioactive molecules, enhancing cell recellularization and differentiation 12,13. Recellularization involves reseeding the decellularized organ with cells. While this process can be performed via perfusion through the renal artery, renal vein, or ureter, there are challenges such as oxygenating cells, recirculating media to preserve nutrients, and ensuring cell viability and migration. Current methods have a low reseeding success rate and difficulty in forming functional renal structures 14–18. This study aims to improve kidney recellularization methods using primary porcine renal cells as a proof of principle. The research demonstrates that perfusion-based decellularization and recellularization can achieve a high cell density in the ECM, low cytotoxicity levels, and the presence of kidney cell markers throughout the kidney. This research could pave the way for similar techniques in human kidneys, offering hope for regenerative medicine in ESRD treatment and improving organ availability for transplantation. Materials and Methods The reporting of this study meets the ARRIVE essential 10 criteria. Experimental Animals and Tissue Collection Our team operates under an organ retrieval animal protocol (A22-0119) sanctioned by the Animal Care Committee of the University of British Columbia (UBC), confirming that all experiments were performed in strict accordance with guidelines and regulations established by the Institutional Animal Care and Use Committee (IACUC) of the University of British Columbia. This protocol ensures compliance with ethical standards and animal welfare principles while facilitating collaboration with existing research endeavors. All kidneys utilized in this study were sourced from adult female Yorkshire pigs (n=19). No animals were specifically procured for sacrifice; rather, the organs were retrieved from pigs involved in other ongoing research protocols upon the conclusion of their respective experiments. Prior to kidney retrieval, pigs were anesthetized by injecting Ketamine S (20mg/kg i.m.), infusing 500ml of lactated Ringer’s solution (1-4 ml/kg/h to prevent hypovolemia, followed by propofol (20-30 mg i.v. in increments). Pigs are then intubated with an endotracheal tube (5.5-6 mm internal diameter). Maintenance of anesthesia is performed by morphine (20 mg i.v.) and propagated by an infusion of propofol (6-8 ml/kg/h). For euthanasia, we inject morphine (1mg/kg i.v.) followed 10 min later by propofol (10mg/kg i.v), rocuronium (1mg/kg i.v.) and subsequent injection of potassium chloride (40mmol) unless otherwise specified by approved animal research protocols. Prior to euthanizing the pigs, 50ml of 10% 1000 USP units/mL sodium heparin (C504036 Fresenius Kabi) was perfused in bolus via IV. Both the renal artery and the renal vein were clamped before removing one kidney, leaving the blood vessels intact for cannulation. The other kidney was removed with blood vessels because it was used for tissue dissociation and cell retrieval. The fresh kidneys were transported in PBS and ice to the biosafety cabinet SterilGard III (GMI). Decellularization Following tissue collection, the kidneys underwent a thorough cleaning process. They were immersed three times for 3 seconds each in 300 mL of sterile ddH2O containing 0.1% hydrogen peroxide (product code 033323 from Fisher Scientific). Subsequently, the organs were rinsed with sterile PBS. To facilitate decellularization, catheterization of the renal vein and artery was performed using a sterile angiocath connected to an intravenous (IV) line (BAX2C8401 from Baxter). Male-to-male luer lock adapters were inserted at both ends of the IV line, and 2-0 silk sutures (J&J-685G Ethicon) were used to secure the tubes in place. Decellularization was achieved through perfusion cycles that alternated between the renal artery and renal vein using a sterilized in vitro bioreactor treated with ethylene oxide. Male Luer lock adapters were connected to the bioreactor valves, which, in turn, were linked to a programmable peristaltic pump (BS-900 from Braintree Scientific Inc.) through a 3/16" ID hose. Seven different methods were explored to optimize kidney decellularization, and the details of each method and their respective results can be found in the supplemental material (Appendix 1). The optimized decellularization protocol (method g) involved the following steps: Perfusion of sterile ddH2O at 10 ml/min for 30 minutes, followed by an increase to 25 ml/min for another 30 minutes. Two cycles of detergents: first, 1% Triton 100X (M143 from VWR) at 25 ml/min for 120 minutes and then at 10 ml/min overnight for 840 minutes. Perfusing 0.1% SDS (BP1311-1 from Fisher Bioreagents) at 25 ml/min for 360 minutes, totaling 45 hours of decellularization. This process took place in a bioreactor with dimensions of 30 cm in height and 25.4 cm in diameter. Conditioning the matrix by perfusing sterile ddH2O at 10 ml/min for one week. A final wash with 1,800 mL of sterile water containing 5% antibiotics and antimycotics 100X (A/A) (#15240062 Gibco, NY USA). Decellularized organs were preserved while awaiting reseeding, using HBSS (product #14025092, Gibco) buffer with 1% A/A (Gibco) at 4°C. To validate the decellularization process, five sections from specific locations of the decellularized kidney and five sections from the same locations of a native kidney were analyzed to quantify the remaining DNA. This analysis was conducted using the DNeasy Blood & Tissue Kit (product #69504, Qiagen, CA, USA), processing 20 mg of tissue and eluting in 80 μL of elution buffer. Histology Sections from the superior pole, inferior pole and close to the renal hilum were extracted in both the cortex and medulla regions to perform the histological analysis of the decellularized and recellularized kidneys. The tissue samples were fixed for 24 hours in 10% neutral buffered formalin (Scigen CA, USA). Afterwards, they were dehydrated in 70% ethanol and embedded in paraffin. Sections of 5um of thickness were mounted on slides deparaffinized in the oven at 60C for one hour and rehydrated in washes of xylene and ethanol to later stain with hematoxylin and eosin (H&E). The slides were examined using SCN400 Slide Scanner (Leica Biosystems, IL, USA) and analyzed with Aperio Digital Whole Slide Scanner (Leica Biosystems). Immunohistochemistry The samples of the recellularized organs were deparaffinized, rehydrated, antigen retrieved, permeabilized and blocked. Afterwards, the slides were incubated with 3% hydrogen peroxide (Sigma-Aldrich, MO, USA) for 10 minutesand washed with PBS. Followed by the incubation with Ki-67 rabbit monoclonal antibody (RM-9106, Thermo Scientific, MA, USA) or with Anti-Cleaved Capsase-3 (ab2302, Abcam, Ma, USA) for 90 minutes at room temperature. The samples were washed with PBS and incubated with Vectastain ELITE ABC-Peroxidase kit (Vector Laboratories, CA, USA) for 30 minutes and washed to incubate later with DAB for 5 minutes. Finally, the slides were washed with ddH 2 O and dehydrated by immersion in ethanol and xylene. The staining intensity and distribution of positive cells were calculated by analyzing 5 sections of the recellularized kidney, using the image analysis software QuPath to quantify cellular proliferation and apoptosis within the recellularized tissue. Proteomics Samples of decellularized and fresh native kidneys were processed for protein extraction. Both groups were minced rigorously and 200 mg of tissue with 200uL of Ripa (R0278 Sigma-Aldrich, MO, USA) and cOmplete™ Protease Inhibitor Cocktail (Sigma-Aldrich) were homogenized in Tissue Grinding tubes with ceramic beads (P000922-LYSK0-A.0, Bertin Instruments, Montignyle-Bretonneux, France) using the Precellys® homogenizer (Bertin Instruments). Afterwards, the supernatant was sonicated (FB705, Fisher Scientific) for milliseconds at 50 of amplitude and centrifuged at max rpm for 4 minutes. The peptide concentration was determined using the Quick Start Bradford Assay (5000205, Bio Rad) according to the manufacturer's instructions and a microplate reader (Epoch, BioTek). The single-pot, solid-phase-enhanced sample-preparation (SP3) method was used for the extraction and processing of the proteins; 100ug of each of the samples of decellularized and native kidney extracts were reduced with 4uL of 200mM 1, 4-Dithiothreitol (DTT, 1019777700, Sigma-Aldrich, 10197777001, Darmstadt, Germany) then alkylated by addition of 4uL of 400mM iodoacetamide (IAA) (90034, Thermo Fisher, MA, USA). SP3 protein clean up and digestion was performed using 1mg (20µl) SeraMag Speedbeads (GE Healthcare) per sample, adding acetonitrile to 70% then washed with 200ul 80% EtOH (x3), and finally suspending in 50mM HEPES pH 8, 2µg trypsin/LycC. After removing the beads, the digests were acidified by the addition of 50µl 0.2% FA Finally, the samples were desalted by solid phase extraction with C18 Sep-Pak cartridges (Waters), conditioned with 1ml acetonitrile/0.1%. The eluate was dried using a centrifugal evaporator (CentriVap) and residue dissolved in a 20ul 0.1% FA. Prepared samples were analysed using an Easy nLC 1200 connected to an Orbitrap Lumos The Easy nLC was set up with a PepMap RSLC C18 75µm x 50cm, 2µm column at 50⁰C using the following gradient: 0-5 min 2% ACN; 5-140min, 2-28% ACN; 140-150min, 28-40% ACN; 150-160min, 40-95% ACN; 160-170min, 95% ACN. Column equilibration was 8µl and sample injects 2µl with 6µl loading volume with total run times about 3hrs. The Easynano source was operated at 2400V and m/z 375-1500 orbitrap (OT) MS1 survey scan data collected at 120000 resolution (profile), RF lense 30%, AGC 4e5, 50ms max. Data dependant MS2 data was collected with peptide monoisotopic selection, 5.0e4 threshold, charge states 2-7, 60 sec dynamic exclusion (10ppm), quad isolation m/z 0.8, HCD activation 30%, OT 15000 resolution (centroid), auto scan range with first mass m/z110, 5.0e4 target with max inject time of 100ms and cycle time of 3sec. Data was processed using Thermo Proteome Discoverer 2.5 with Sequest and the Sus Scrofa dataset (Sus Scrofa TaxID=9823, 2022-03-02 and Homo SapiensTaxID=9606) with the following parameters: precursor mass 350-5000 Da; enzyme, trypsin; missed cleavage, 2; min/max peptide length 6/144; precursor/fragment tolerance, 10ppm/0.02Da; all neutral losses; b,y ions only; static modification, carbamidomethyl; dynamic modifications, oxidation (K, M, P), protein terminus acetyl; Cn max 0.05; PSM, peptide and decoy DB target FDR’s, 0.01 strict, 0.05 relaxed, based on q-value. Filtering was set for peptides at least medium confidence however only high confidence data was selected with final post process filtering with Discoverer. Protein and Protein Group were exported to Excel for further sorting and analysis. Primary cell culture The cells used for recellularization were obtained from fresh kidneys of pigs undergoing euthanasia in our Animal facility under our animal protocol (A22-0119) approved by the University of British Columbia. To obtain a population of cells labelled as whole kidney pig cells (WKPC) the organ was minced and weighted ∼5.5 mg was dissociated with Collagenase A (10103586001, Sigma-Aldrich) at 2.25 mg/ml and Dispase II (17105-041, Gibco) 10U in a mixture of HBSS and DMEM an incubated at 37C for two hours gently shaking the mixture continuously. The mixture was filtered through a 70um and 40uM cell strainer and centrifuged to later be incubated 10 minutes with TrypLE (12605028, Gibco). The culture was monitored and expanded until passage five at 37 °C and 5% CO 2 in 175cm flasks, using kidney mix media (KMM) supplemented with 5.1% of FBS (Hyclone, UT, USA) and 5% A/A, the composition consisted of one third of DMEM (11965092, Gibco), one third of REBM (CC-3190, Lonza, GA, USA) supplemented with 0.1% hEGF, 0.1% Insulin, 0.1% Hydrocortisone, 0.1% GA-1000, 0.1% Transferrin, 0.1% Triiodothyronine, 0.1% Epinephrine, and one-third of EGM (CC-3162, Lonza), supplemented with 0.04% Hydrocortisone, 0.4% hFGF-B, 0.1% VEGF, 0.1% R3-IGF-1, 0.1% Ascorbic Acid, 0.1% hEGF, 0.1% GA-1000, 0.1% Heparin. The passage was done when the cells were at 80% confluency with 0.25% Trypsin-EDTA (#25200056, Gibco, MD, USA) for 10 minutes at 37°C was used for detachment and with later passages, a cell scrapper was needed. WKPC were frozen in liquid nitrogen with Bambanker (BB01, Cederlane) in cryovials for better preservation. The number of cells were determined with the Automated Cell Counter TC20 (Bio-Rad, WA, USA). Flow cytometry Primary porcine renal cells were used with and without culture directly after cell dissociation. Expanded WKPC cells were detached using Cell Stripper (VWR, Cat# CA4500-668), a non-enzymatic reagent washed with PBC and resuspended in FACS buffer (1X PBS, 2.5mM EDTA, 2% FBS, 0.05% sodium aside). To analyze for viability, The Zombie NIR™ dye (423107, BioLegend, CA, USA) in a 1:100 dilution was added to the cells and incubated for 15 minutes. For blocking, the cells were incubated on ice with Fc block (564220, BD biosciences) diluted in a1:300 for 5 minutes. For surface staining, the primary antibodies were added and incubated on ice, in the dark for 45 minutes. Due to the lack of antibodies for pig samples, anti-human antibodies were used with predicted reactivity with pig. The following primary antibodies were used: anti-human CD14-AF700 (301822, BioLegend), anti-mouse/human E-Cadherin-BV421 (147319, Bio Legend), anti-mouse/human AQP1-AF594 (bs-1506R-A594, Bioss USA, MA, USA), anti-mouse/human AQP4-APC-Cy7 (bs-0634R-APC-Cy7, Bioss USA), anti-mouse/human Podocin-PE (bs-6597R-PE, Bioss USA), anti-human CD31-APC (303115, BioLegend). After washing, the cells underwent intracellular protein staining, involving fixation, permeabilization, and incubation in the dark with Anti-Human Vimentin-AF488 (562338, BD Biosciences) for 15 minutes. Finally, the cells were filtered, resuspended in FACS buffer, and analyzed by FACS Canto II Flow Cytometry System (BD Bioscience). The percentage of positively stained cell count was quantified using FlowJo_V10. Quantitative real-time PCR (RT-qPCR) WKPC from every passage (2 x 10 6 cells) were washed with 100 uL of PBS and then stored in 100uL of RNA later at - 20°C. The total RNA was extracted with the RNeasy kit (Qiagen) following the manufacturer's instructions. The cDNA synthesis was performed using 1000ng of RNA and the High Capacity cDNA Reverse Transcription Kit (4368813 Applied Biosystems, CA, USA). The analysis of real time PCR (RT-PCR) was done QuantStudio 7Pro (Applied Biosystems) using PowerTrack™ SYBR™ Green Master Mix (4309155 applied biosystem). The reaction was carried out with an initial denaturation of 2 minutes followed by 40 cycles of denaturation of 95 for 5 seconds and elongation at 60 for 35 seconds. Using 300nM of gene-specific designed primers for cell kidney markers and GADPH as a housekeeping gene for normalization and quantifying gene expression which can been found in the supplemental material (Appendix 2). Recellularization The tubbing, Luer lock adaptors and the bioreactor were sterilized using ethylene oxide. The decellularized organ was connected through the renal vein and the renal artery to the valves of the bioreactor employing the same IV lines that were used for decellularization, connecting them with Luer lock adapters to the valves of the bioreactor; the assembling, sterilization and cell perfusion was done on the biosafety cabinet. For the sterilization of the organ, ddH 2 O with 1% A/A was perfused through the renal artery and renal vein at 10ml/min using a peristaltic pump (BS-900 Braintree Scientific Inc.), after the continuous perfusion 150ml of ddH 2 O with 10% A/A were left inside the organ for 2 hours. Using a syringe 40 × 10 6 WKPC were infused into the renal artery resuspended in 45 ml of DMEM 10% FBS and 1% A/A, 40 × 10 6 WKPC perfused through the renal vein and 20 × 10 6 WKPC perfused through the ureter, previously catheterized, -17inHg of negative pressure was applied after 10 minutes in static state, only during the first day of cell perfusion. Five identical cycles of cell perfusion were done having a total of 500 × 10 6 WKPC. DMEM 10% FBS and 1% A/A, was perfused through the renal artery every 24 hours for four days for 1 hour at 10ml/min. At the end of the fourth day, the bioreactor was set up in continuous recirculation of supplemented DMEM at 10ml/min inside an incubator for 8 days, the media was changed every day and oxygenated by agitation for one hour daily. On the ninth day, oxygenation was provided using red blood cells (RBC) extracted isolated from human’s blood employing Ficoll-Paque PLUS (#17144003, Cytivia Life Sciences). The RBC are mixed with the media and perfused into the kidney with daily oxygenation by agitation. The media was stored everyday using cOmplete™ Protease Inhibitor Cocktail (Roche, Germany) at -80C, later on it was analyzed to count and collect the number of cells that flowed out of the kidney, obtaining the retention of cells following the methodology proposed by Hochman-Mendez 19 . On the twelfth day the kidney was sectioned and prepared for histology analysis. LDH Assay The media obtained after the recellularization was thawed and analyzed using the LDH-Glo™ Cytotoxicity Assay (Promega, WI, USA) according to the manufacturer's instructions, using 2μl of 10% Triton X-100 per 100μl of media as a negative control. The assay was done with 25uL of samples and 60 minutes of incubation time, and luminescence was recorded using a luminometer infinite m200 pro (Tecan, Switzerland) Immunofluorescence For the analysis of cells, WKPC’s were cultured in Nunc™ Lab-Tek™ Flask on Slide (170920, Thermo Fisher, MA, USA)) and were allowed to grow for 72 hours, washed with 1X filtered PBS and fixed with 4% paraformaldehyde (Sigma-Aldrich, MO, USA). Regarding the study of the recellularized kidney sections, five slides of the inferior pole with longitudinal cut were deparaffinized, once rehydrated they were treated with Diva Decloaker 10X (Cedarlane, ON, CA) for 30 mins in boiling water for the antigen retrieval, then washed with dH 2 O. Both Sections and cell’s slides were washed with PBS, permeabilized with 0.3% Triton X-100. incubated with Fc blocker and blocked for non-specific binding with 2.5% horse serum (Vector Laboratories, CA, USA) for 30 minutes. Slides were stained with Mouse Anti-E-Cadherin at 1:500 (610181 BD Bioscience), mouse anti-Aquaporin-1 AT 1:400 (ab9566 ), mouse anti-CD31 at a1:100 (ab24590), rabbit anti-Vimentin at 1:200 (mAb 5741, rabbit anti-aquaporin-1 at 1:500(mAb59678 ), rabbit anti-podocin at 1:500 (ab216341) antibodies incubated overnight at 4 °C. Goat Anti-Mouse IgG (Alexa Fluor 569) and Goat Anti-Rabbit IgG (Alexa Fluor 488) were used as secondary antibodies for staining at a 1:200 dilution for two hours in the dark, the slides were mounted with DAPI (Vector Laboratories, CA, USA) and imaged with confocal microscope at 20X and 60X magnifications (Olympus FV3000RS). Statistical Analysis R studio (2021.09.0) was used to analyze the statistical difference between two groups using a paired t-test. It was considered statistically different when the probability value P <0.05. Data were presented as mean±S.D Results Decellularization The primary objective was to minimize the exposure of the extracellular matrix (ECM) to detergents by adjusting the flow rates, rather than altering the duration of detergent exposure in each cycle. We successfully established a decellularization protocol for pig kidneys using perfusion, achieving complete decellularization within 45 hours (Fig. 1 ). Macroscopic evaluation demonstrated effective cell removal through detergent perfusion via the renal vein and renal artery, resulting in partially transparent decellularized organs that retained their structures and vascular networks. Consistent with the macroscopic assessment, microscopic evaluation of various sections of the decellularized organs using H&E staining revealed complete cell removal in the renal medulla and cortex of pig kidneys. This preservation of the organ's microarchitecture indicated the production of a high-quality ECM suitable for recellularization. In the medulla, the characteristic honeycomb-like appearance was maintained (Fig. 2 A), while in the cortex, the loops corresponding to the glomerulus, a crucial kidney component, were preserved (Fig. 2 B). Double-stranded DNA content was quantified and compared between decellularized kidneys (12.24 ng/µl ± 4.07 ng/µl) and native kidneys (1113.17 ng/µl ± 100.02 ng/µl), revealing a statistically significant difference with a p-value of < 0.0001 (1.578*10^-05). This difference signifies a reduction of over 99% in residual DNA in the decellularized pig kidneys, suggesting the elimination of the majority of cells and any remaining DNA (Fig. 2 C). Proteomic analysis of the extracellular matrix Out of the 4,620 proteins identified in native pig kidneys, 3,162 proteins were found in the decellularized pig kidneys. A comparison between the decellularized and native kidneys revealed an overlap of 2,715 proteins, with 447 proteins uniquely identified in the decellularized tissue and 1,895 proteins exclusively detected in the native kidney. The absence of certain proteins in the decellularized kidney can be attributed to the removal of cellular components, including enzymes and organelle-specific proteins. It's important to note that the samples were derived from different kidneys. Table 1 presents a total of 170 proteins, which include critical components of the extracellular matrix. Notably, the decellularized organ exhibited the presence of seven different types of growth factors. The number of peptides corresponds to the unique peptides identified per protein by the mass spectrometer, while coverage indicates the proportion of amino acids covered by the identified peptides relative to the total protein length. Abundance reflects the combined intensity of all analyzed peptides, serving as a marker for quantifying protein relative abundance in the organ 20,21 . The decellularized kidney displayed high concentrations of key proteins such as collagen IV, collagen V, fibronectin, elastin, glycoproteins, integrin, laminin, and fibrillin. These proteins play a critical role in establishing a functional scaffold, preserving cell viability, and facilitating cellular recruitment 12,22,23 . The fold rate indicates changes in relative protein concentration during the decellularization process. Due to the removal of cellular components, some proteins showed increased relative concentrations in the decellularized organ, as indicated by fold rates greater than 1. Notably, certain growth factors exhibited higher relative concentrations in the decellularized kidney, including acidic fibroblast growth factor (FGF), epidermal growth factor, and fibroblast growth factor. Furthermore, the decellularized scaffold retained 64% of VEGH compared to the native kidney. This growth factor is crucial for kidney bioengineering as it plays a role in angiogenesis and renal podocyte development 24 . Table 1 Proteomic analysis of decellularized and native pig ( Sus scrofa ) kidney Accession number Description # Peptides (Native) Coverage [%] (Native) Abundance (Native) # Peptides (Decellularized) Coverage [%] (Decellularized) Abundance (Decellularized) Fold F1RU24 Acidic fibroblast growth factor 4 58 2.33E + 06 2 41 3.11E + 06 1.33 A0A287ANW0 Actin alpha 2, smooth muscle 19 68 2.10E + 10 16 63 6.60E + 09 0.31 F1STD5 Actin related protein 1B 7 19 2.63E + 05 5 20 5.35E + 06 20.32 A0A480NK33 Actin related protein 2 12 36 2.17E + 08 11 33 2.70E + 08 1.25 A0A286ZIH7 Actin related protein 3 17 50 2.76E + 08 14 37 2.10E + 08 0.77 I3LHK5 Actin-related protein 10 3 10 2.89E + 06 3 12 3.27E + 06 1.13 B5APU6 Actin-related protein 2/3 complex subunit 8 27 7.04E + 07 11 44 2.60E + 08 3.66 Q6QAQ1 Actin, cytoplasmic 1 18 63 1.12E + 10 16 67 5.60E + 09 0.5 I3LLY3 Actinin alpha 1 41 56 8.32E + 08 35 51 1.50E + 08 0.18 F1RI39 Actinin alpha 4 56 71 5.15E + 09 46 61 1.50E + 09 0.29 A0A286ZR45 Adducin 1 18 35 1.38E + 08 7 14 4.50E + 07 0.32 A0A287AQ41 Adducin 3 17 38 1.07E + 08 6 13 4.80E + 07 0.45 I3LI80 Advillin 3 6 2.12E + 06 4 25 5.55E + 05 0.26 F1SSF7 AE binding protein 1 13 14 6.59E + 07 11 13 3.10E + 07 0.47 A0A286ZS39 Agrin 37 31 1.90E + 08 32 23 3.20E + 08 1.7 A0A5G2QI26 Alanyl aminopeptidase, membrane 35 47 3.66E + 09 22 36 2.20E + 08 0.06 F2Z5G5 Alpha-centractin 11 47 9.17E + 07 9 40 5.60E + 07 0.61 Q7M2W6 Alpha-crystallin B chain 9 58 1.08E + 08 4 21 7.93E + 06 0.07 P79307 Amyloid-beta precursor protein 6 10 9.43E + 06 2 23 8.41E + 05 0.09 F1SJB5 Annexin 16 56 4.26E + 08 7 16 4.10E + 07 0.1 P19620 Annexin A2 22 64 1.19E + 09 17 57 1.10E + 08 0.09 F1SUE4 Asporin 3 11 9.91E + 06 7 25 3.40E + 07 3.42 A0A5G2QGZ8 Basigin 10 33 3.66E + 08 3 12 1.60E + 07 0.04 A0A5K1UKC5 Biglycan 11 44 1.66E + 08 9 37 1.60E + 08 0.94 F1SAM3 Cadherin 2 10 17 3.17E + 07 3 15 6.01E + 06 0.19 A0A5G2QAL2 Calponin 9 37 8.41E + 07 5 12 1.60E + 07 0.19 A0A5G2R7Z4 Catenin alpha 1 39 59 7.15E + 08 11 16 5.10E + 07 0.07 F6QAP1 Catenin beta 1 23 40 3.20E + 08 13 20 3.10E + 07 0.1 F1SGT4 CD44 antigen 5 8 2.49E + 07 2 11 1.83E + 06 0.07 A0A286ZRZ8 Cingulin like 1 19 16 1.65E + 08 5 5 7.58E + 06 0.05 F1RP52 Claudin 2 6 1.89E + 06 2 6 3.64E + 05 0.19 A0A287A1S6 Collagen alpha-1(I) chain preproprotein 49 52 1.57E + 09 56 61 4.10E + 10 26.34 A0A480I6I0 Collagen alpha-1(XIV) chain 63 51 2.16E + 09 69 55 5.80E + 09 2.68 Q59IP2 Collagen alpha-2(V) chain 3 3 2.11E + 06 26 29 3.40E + 08 159.78 F1SFA7 Collagen type I alpha 2 chain 46 49 9.66E + 08 49 54 7.40E + 09 7.68 A0A287A2Q7 Collagen type II alpha 1 chain 3 3 5.48E + 06 12 11 7.90E + 06 1.44 A0A286ZQ85 Collagen type III alpha 1 chain 50 47 1.37E + 09 54 55 1.20E + 10 8.71 A0A8W4FCC2 Collagen type IV alpha 1 chain 9 10 1.20E + 08 17 18 1.10E + 10 94.13 F1RLL9 Collagen type IV alpha 2 chain 21 20 1.82E + 08 35 37 9.70E + 09 53.18 F1SNP0 Collagen type IV alpha 3 chain 2 1 1.53E + 07 11 11 1.30E + 09 86.34 A0A287AZF2 Collagen type IV alpha 4 chain 14 13 1.06E + 08 14 13 1.10E + 08 1 A0A287A007 Collagen type IV alpha 5 chain 12 11 2.02E + 08 12 11 2.00E + 08 1 A0A8W4FGE1 Collagen type IV alpha 6 chain 4 3 6.76E + 06 15 13 2.60E + 08 38.88 F1S021 Collagen type V alpha 1 chain 7 6 3.78E + 07 18 15 4.60E + 08 12.07 I3LS72 Collagen type VI alpha 1 chain 37 49 1.21E + 09 38 53 9.10E + 09 7.54 I3LQ84 Collagen type VI alpha 2 chain 26 35 1.25E + 09 31 41 5.50E + 09 4.39 A0A286ZVG7 Collagen type VI alpha 3 chain 93 41 3.21E + 09 91 44 1.10E + 10 3.5 A0A287BK35 Collagen type VI alpha 5 chain 47 24 3.02E + 08 61 31 9.80E + 08 3.23 A0A5G2R0Y2 Collagen type VI alpha 6 chain 5 3 1.15E + 07 20 11 7.30E + 07 6.32 F1SKM1 Collagen type VII alpha 1 chain 22 10 1.06E + 08 22 10 1.10E + 08 1 F1SKX7 Collagen type VIII alpha 1 chain 3 5 3.65E + 07 3 5 3.60E + 07 1 A0A5G2RKA4 Collagen type XI alpha 1 chain 5 4 3.66E + 06 5 4 3.66E + 06 1 F1RQI0 Collagen type XII alpha 1 chain 112 51 2.82E + 09 118 56 4.80E + 09 1.69 F1SSE8 Collagen type XV alpha 1 chain 16 15 2.57E + 08 12 13 1.10E + 08 0.42 A0A5G2QW73 Collagen type XVI alpha 1 chain 2 2 1.32E + 06 5 5 8.62E + 06 6.54 A0A286ZIL9 Collagen type XVIII alpha 1 chain 26 19 4.62E + 08 14 11 1.10E + 08 0.24 F1RZU6 Collagen type XXI alpha 1 chain 3 3 5.16E + 06 3 3 5.16E + 06 1 A0A8W4FDC1 Collagen type XXIV alpha 1 chain 2 2 3.79E + 07 2 2 3.80E + 07 1 A0A8W4FA02 Collagen type XXVI alpha 1 chain 2 4 6.14E + 06 2 4 6.14E + 06 1 A0A286ZI28 Collectrin 6 36 5.67E + 07 6 31 4.60E + 07 0.82 P02540 Desmin 33 72 1.78E + 09 19 39 1.10E + 08 0.06 Q5GN48 Dystrophin 11 5 1.51E + 07 2 1 4.36E + 06 0.29 A0A287BJX5 Epidermal growth factor receptor 3 43 2.48E + 07 2 25 3.39E + 07 1.37 A0A287AP71 EGF containing fibulin extracellular matrix protein 1 8 20 7.42E + 06 11 30 9.30E + 07 12.51 A0A5G2QCU4 Elastin microfibril 21 25 1.56E + 08 20 21 1.30E + 08 0.86 A0A287AXU0 Elastin 2 7 2.96E + 07 2 37 8.25E + 07 2.79 I3LC64 Extracellular matrix protein 1 4 12 7.29E + 06 3 8 6.79E + 06 0.93 A0PFK5 F-actin-capping protein subunit alpha-1 12 69 8.74E + 07 4 20 3.41E + 06 0.04 Q29221 F-actin-capping protein subunit alpha-2 9 51 2.12E + 07 4 20 7.22E + 05 0.03 A0PFK7 F-actin-capping protein subunit beta 9 36 1.30E + 08 4 14 1.50E + 07 0.12 F1RQ01 FERM domain containing kindlin 3 3 7 2.21E + 06 6 12 7.17E + 06 3.24 F1SN67 Fibrillin 1 78 40 1.03E + 09 99 49 7.00E + 09 6.85 A0A5G2QM06 Fibrillin 2 7 3 6.52E + 05 45 22 1.80E + 08 283.62 F1RX36 Fibrinogen alpha chain 21 26 5.71E + 08 18 22 5.00E + 08 0.88 I3L651 Fibrinogen beta chain 24 61 8.66E + 08 20 56 1.00E + 09 1.21 A0A5G2QUU1 Fibrinogen gamma chain 23 67 7.29E + 08 24 68 1.70E + 09 2.38 A0A287BGK8 Fibroblast growth factor 2 3 4.85E + 05 4 73 1.45E + 06 2.98 F1S6B5 Fibromodulin 2 9 1.52E + 07 7 27 6.70E + 07 4.41 F1SS24 Fibronectin 66 44 1.01E + 09 61 37 1.00E + 09 1.01 F1SPG5 Fibulin 2 6 8 9.53E + 06 8 8 2.50E + 07 2.62 A0A287AL89 Fibulin-1 9 16 2.09E + 07 12 22 8.70E + 07 4.17 A0A480ZQ94 Fibulin-5 9 24 1.22E + 08 7 16 1.20E + 08 0.96 A0A287AW81 Filamin A 108 58 4.17E + 09 29 12 2.70E + 08 0.06 A0A287BLE0 Filamin B 99 52 8.70E + 08 13 6 1.70E + 07 0.02 F1SMN5 Filamin C 37 18 5.12E + 07 11 5 1.81E + 06 0.04 A0A5G2Q797 Galectin 6 33 2.19E + 08 6 34 1.40E + 08 0.66 A0A287A6P1 Gelsolin 23 41 5.42E + 08 5 8 9.53E + 06 0.02 F1RX94 Growth factor receptor bound protein 7 2 8 2.51E + 06 2 32 6.54E + 05 0.26 F1SEQ3 Growth hormone-inducible transmembrane protein 6 15 1.15E + 08 4 12 3.20E + 07 0.28 A0A286ZHV7 Heparan sulfate proteoglycan 2 105 39 1.35E + 09 106 39 3.80E + 09 2.83 A0A481B9A6 Histidine-rich glycoprotein 21 44 9.45E + 08 6 16 2.50E + 07 0.03 K7GQ83 Integrin 25 37 8.69E + 08 12 26 2.24E + 08 0.26 F1SB67 Insulin like growth factor 2 receptor 19 10 4.14E + 07 12 75 1.50E + 07 0.37 F1RVH7 Insulin like growth factor binding protein 7 10 51 5.44E + 07 9 41 9.40E + 07 1.73 A0A287AG36 Laminin subunit alpha 1 15 7 8.11E + 07 18 7 3.90E + 07 0.48 A0A287A027 Laminin subunit alpha 2 8 6 6.86E + 06 8 5 7.43E + 06 1.08 F1RZM4 Laminin subunit alpha 4 18 14 5.03E + 07 5 3 9.39E + 06 0.19 A0A8W4FFH5 Laminin subunit alpha 5 52 21 1.92E + 08 95 37 1.50E + 09 7.62 F1SAE9 Laminin subunit beta 1 36 28 2.31E + 08 52 42 1.10E + 09 4.68 A0A287AWV5 Laminin subunit beta-2 6 4 1.03E + 07 33 24 1.60E + 08 15.43 F1S663 Laminin subunit gamma 1 43 42 2.84E + 08 47 41 1.00E + 09 3.55 A0A286ZWQ1 Latent transforming growth factor beta binding protein 1 7 6 3.27E + 06 11 9 2.70E + 07 8.21 A0A5G2R0I5 Latent transforming growth factor beta binding protein 4 12 12 1.93E + 07 3 3 1.66E + 06 0.09 F1S3D5 Lectin, mannose binding 2 6 24 5.68E + 07 2 3 1.17E + 06 0.02 B0KYV5 LIM domain and actin-binding protein 1 17 33 6.07E + 07 3 4 7.14E + 06 0.12 F1SQ09 Lumican 11 44 2.00E + 08 10 42 3.70E + 08 1.87 I3LQ79 Major vault protein 34 48 4.40E + 08 25 34 2.90E + 08 0.65 I3LLG6 Matrix remodeling-associated protein 8 2 7 2.03E + 06 2 13 5.97E + 05 0.29 F1RS52 Metabolism of cobalamin associated A 7 15 1.66E + 07 5 13 1.00E + 07 0.63 F1SUS0 Microfibril associated protein 2 2 16 5.39E + 06 4 25 7.40E + 08 138.13 A0A287AB52 Microfibril associated protein 4 2 9 2.78E + 07 4 20 6.20E + 07 2.23 A0A5G2R2Y0 Microfibril associated protein 5 4 14 2.62E + 07 3 10 4.95E + 06 0.19 A0A287AK19 Moesin 32 51 3.10E + 08 16 28 5.00E + 07 0.16 M3UZ63 Myeloid derived growth factor 5 32 3.72E + 07 4 23 2.90E + 07 0.78 A0A8W4FCU2 Myosin heavy chain 9 115 57 3.53E + 09 62 37 2.10E + 09 0.6 A0A287BFH0 Myosin IB 10 11 2.82E + 07 10 10 1.50E + 07 0.53 A0A480I6L4 Myosin IC 34 41 2.74E + 08 27 32 1.20E + 08 0.44 A0A287ARN5 Myosin ID 9 11 1.77E + 07 13 15 3.10E + 07 1.75 F1SA47 Myosin IF 4 5 5.91E + 08 6 6 1.00E + 07 0.02 F1SM78 Myosin light chain 12B 12 63 1.16E + 09 9 62 1.10E + 08 0.09 F1SSA6 Myosin motor domain-containing protein 53 56 4.94E + 08 28 31 6.10E + 07 0.12 P29269 Myosin regulatory light polypeptide 9 12 63 2.30E + 08 9 62 1.10E + 07 0.05 A0A5G2R6M1 Myosin VB 11 9 1.68E + 07 15 9 3.00E + 07 1.77 A0A287ARB4 Myosin VC 5 3 4.05E + 06 7 4 6.10E + 06 1.51 F1SUK5 Myosin VIIA 6 4 4.31E + 05 21 10 4.40E + 07 102.83 A0A287AMU3 Myosin-10 10 49 5.92E + 07 7 39 8.40E + 06 0.14 F1SF23 Nephrocan 3 1 2.86E + 06 2 3 1.60E + 07 5.57 A0A287AU52 Nephronectin 3 6 2.49E + 06 12 22 1.50E + 08 58.74 F1RGY5 Nidogen 1 22 29 1.38E + 08 20 22 2.00E + 08 1.45 A0A8W4FJT4 Nidogen 2 26 30 1.99E + 08 21 23 2.30E + 08 1.16 F1S6A2 NPHS2 stomatin family member, podocin 5 14 2.19E + 07 5 13 3.30E + 07 1.51 A0A8W4FNH8 Opioid growth factor receptor (OGFr) 4 3 9.64E + 06 6 61 2.22E + 06 0.23 A0A0H5ANC0 Osteoglycin 9 31 1.26E + 08 5 20 2.10E + 07 0.17 F1S3J7 Papilin, proteoglycan like sulfated glycoprotein 16 27 4.95E + 07 4 7 5.86E + 06 0.12 A0A5G2QJH9 Parvin alpha 6 19 6.74E + 07 5 16 1.70E + 07 0.25 A0A5G2RG25 Paxillin 6 7 2.17E + 07 4 4 6.39E + 06 0.29 F1RS37 Periostin 6 10 6.96E + 06 4 7 6.05E + 06 0.87 F1RL75 Platelet-derived growth factor receptor beta 10 12 1.71E + 07 3 2 1.12E + 06 0.07 A0A287A8C7 Plectin 115 28 5.81E + 08 25 5 5.50E + 07 0.09 F1SNF3 Podocalyxin 7 20 7.72E + 07 2 3 4.77E + 06 0.06 I3LEB7 Podocan 7 7 4.23E + 08 5 8 1.80E + 07 0.04 I3LEE6 Procollagen C-endopeptidase enhancer 2 6 1.68E + 06 2 6 1.68E + 06 1 A0A8W4FAZ5 Radixin 37 54 1.78E + 09 18 34 1.00E + 09 0.59 F1S4R1 Regulator of microtubule dynamics 2 5 19 4.76E + 06 3 8 6.29E + 06 1.32 A0A287BBC9 Regulator of microtubule dynamics 3 5 13 1.32E + 07 2 7 2.33E + 06 0.18 A0A286ZRU9 Serpin H1 18 50 8.77E + 08 13 35 1.50E + 08 0.17 P20112 SPARC 6 20 1.40E + 07 3 9 2.50E + 07 1.77 F1S981 Spondin-1 6 12 8.33E + 06 4 6 4.30E + 06 0.52 A0A8W4FGV4 Synemin 8 26 1.81E + 07 3 8 3.17E + 06 0.18 A0A5G2Q9C7 T-complex protein 1 subunit gamma 26 63 4.05E + 08 23 52 2.40E + 08 0.59 A0A287BAK1 Talin 1 100 57 1.11E + 09 16 8 3.60E + 07 0.03 A0A287B272 Talin 2 42 21 6.14E + 07 4 2 1.16E + 06 0.02 I3LUI4 Tenascin C 30 20 6.29E + 07 13 8 9.28E + 05 0.01 K7GRK7 Tenascin XB 24 12 2.56E + 07 31 15 9.20E + 07 3.6 Q2QLE3 Testin 5 15 1.46E + 07 2 5 1.97E + 06 0.13 F1SS26 Thrombospondin 1 21 27 7.63E + 07 8 8 7.35E + 06 0.1 F1SIR7 Thrombospondin type 1 domain containing 4 2 3 5.14E + 05 5 6 1.20E + 07 23.75 F1RHA7 Transforming growth factor beta induced 18 41 1.46E + 07 13 28 9.03E + 06 0.62 A0A287B6W1 Transforming growth factor beta-1-induced transcript 1 protein 2 6 4.60E + 06 2 3 7.73E + 06 1.68 O11780 Transforming growth factor-beta-induced protein ig-h3 18 41 1.55E + 08 13 27 3.30E + 08 2.15 A0A287AZK2 Transgelin 14 51 9.78E + 08 9 36 1.10E + 08 0.11 P67937 Tropomyosin alpha-4 chain 17 47 5.47E + 08 7 28 3.30E + 07 0.06 I3LGB4 Tubulointerstitial nephritis antigen 13 37 2.20E + 08 19 53 1.30E + 09 5.91 F1RPA9 Uromodulin 19 34 3.92E + 08 8 12 2.80E + 07 0.07 A0A287BFL9 Vascular endothelial growth factor 4 59 1.05E + 08 24 38 6.79E + 07 0.64 F1SX62 Versican 10 26 3.19E + 07 3 5 7.47E + 06 0.23 Q29261 Villin-1 31 49 9.23E + 08 20 29 3.70E + 08 0.4 P02543 Vimentin 39 79 5.09E + 09 21 40 6.40E + 08 0.13 P48819 Vitronectin 10 31 1.73E + 08 7 19 4.00E + 07 0.23 Characterization of the primary porcine renal cells During the primary culture of WKPC with kidney mix media (IP under submission), we observed various morphologies, primarily during early passages. However, with successive passages, the heterogeneity of the cell population decreased, and fibroblast-shaped cells began to dominate the culture, reaching confluency earlier with each passage. Analysis of WKPC revealed changes in marker expression over successive passages. Both qPCR and immunofluorescence confirmed the presence of epithelial cells expressing CDH1 (E-cadherin), AQP1 indicative of cells found in proximal tubules, and AQP4 indicating the presence of cells conforming to the collecting ducts 25 . Additionally, cells expressing Vimentin, typically found in the renal capsule, glomeruli, and vasculature, were identified, suggesting the presence of renal tubular cells and fibroblasts 26,27 . The analysis also revealed the presence of endothelial cells expressing CD31 and podocytes expressing podocin, crucial for glomeruli formation, the site of the initial filtration step 25,28,29 . Furthermore, a lower expression of immune cells, particularly monocytes expressing CD14, was observed 30 (Fig. 2 a). RT-qPCR analysis demonstrated an upregulation of CDH1 and Vimentin as passages increased, compared to uncultured WKPC, indicating a higher presence of epithelial and fibroblast cells in the culture (Fig. 2 b). In contrast, the expression of AQP1, AQP4, CD14, CD31, and Podocin decreased compared to the original uncultured WKPC. The most significant decrease was observed in immune cells expressing CD14. Notably, podocin exhibited a lower decrease in expression compared to the other markers (Fig. 2 a). These findings suggest that WKPC should be utilized at earlier passages, preferably up to passage two, to ensure effective repopulation with a variety of cells as found in the native kidney. These results were confirmed with immunofluorescence analysis. The majority of the cells showed positive staining for AQP1, AQP4, Vimentin, and CDH1 (E-cadherin), indicating their epithelial cell nature. A small percentage of the population demonstrated positive staining for CD31 and podocin, suggesting the presence of endothelial cells and podocytes, respectively (Fig. 2 c-g). These findings validate the efficacy of the method used to isolate WKPC, which involved the dissociation of kidney sections rather than utilizing the whole organ. By using this approach, we generated populations of cells expressing a diverse range of kidney-specific markers, crucial for performing various kidney functions. This highlights the potential of WKPC as a valuable resource for further studies to optimize the recellularization techniques of a whole pig kidney and later test for functionality both in vivo and in vitro. Flow cytometric analysis corroborated what was found in the RT-qPCR assay, showing that the WKPC population changes over passages. Table 2 provides marker expression percentages for different renal cell antibodies in WKPC at various passages. Passage zero represents cells without culture, freshly isolated from a native kidney. The cell viability of WKPC remained constant through passages, suggesting that fluctuations in percentages over passages might be due to the faster duplication rates of stromal and fibroblast cells expressing vimentin rather than cell death 31, 32 . In cells obtained directly from the tissue (P0), a predominance of epithelial cells expressing AQP4 and AQP1 was observed, along with a significant percentage of endothelial cells. However, by passage 3, there was a notable shift in cell composition. Most cells expressed Vimentin, indicative of a stroma or fibroblast-like phenotype, while the percentages of AQP1 and CD31-positive cells decreased. Results showed that Vimentin expression gradually increased from 15.20% in passage 0 to 66.10% in passage 3, indicating an enrichment of Vimentin-positive cells over successive passages, in agreement with RT-qPCR results. This change may be attributed to culture conditions favoring the growth of Vimentin-expressing cells. In contrast, Podocin expression increased from 13.70–47.90% in passage 2 and then slightly decreased to 37.60% in passage 3. Moreover, the expression of CD14, a marker associated with immune cells, remained relatively low throughout the passages, ranging from 0.12–2.08%. The expression of AQP1 decreased from 65.30% in uncultured cells to 22.20% in passage 3, indicating a potential loss or reduced presence of proximal tubular cells. CD31, a marker for endothelial cells, decreased from 65.90–22.40% in passage 3. AQP4, a marker for collecting duct cells, showed a slight decrease from 69% in passage 0 to 50.80% in passage 3. These findings highlight the heterogeneity and phenotypic changes within the WKPC population during culture, underscoring the importance of considering passage number when utilizing these cells for recellularization or other applications. Table 2 Results of the flow cytometry analysis of whole kidney pig cells (primary renal porcine cells) Fluorophore Marker P0 P1 P2 P3 FITC Vimentin 15.2% 44.1% 56% 66.1% PE Podocin 13.7% 40.0% 47.9% 37.6% Per-CP CD14 2.08% 0.18% 0.12% 0.33% PE-Cy7 AQP1 65.3% 25.5% 37.1% 22.2% APC-Cy7 CD31 65.9% 29% 33.4% 22.4% APC-Cy7 AQP4 69% 36% 52% 50.8% Zombie UV Viability 98.5% 93.0% 97.7% 97.5 Evaluation of a whole Recellularized pig kidney The accompanying image showcases the macroscopic transformation of the kidney over the course of several days (Fig. 3 b). Starting from a decellularized organ, the kidney progressively evolved to contain 500 million well-characterized WKPC and was initially perfused solely with media. Ultimately, the kidney contained an impressive population of 318 billion red blood cells (RBCs). It is worth noting that the proportion of WKPC within the total cell population accounted for only 0.15% of the perfused RBCs, leading to erythrocyte accumulation all over the matrix. However, prior to the perfusion of RBCs, on the 5th day, the kidney closely resembled a native kidney, without blood flow. In the panel of Fig. 3 a it is shown six sections representing the histological examination of a native kidney, a decellularized kidney, and a recellularized kidney. Starting from the left, the native kidney section serves as a reference for comparison. It displays the characteristic histological features of a healthy kidney, including well-defined renal structures such as glomeruli, tubules, and blood vessels. The decellularized kidney displays a significant absence of cellular material while keeping the ECM intact. On the right side, we have the recellularized kidney section, resembling every time more a native kidney. This section demonstrates a high percentage of repopulation of the decellularized ECM with new cells. The image reveals a reestablishment of cellular density and organization. Regions with clusters of cells, forming cell colonies, can be observed. Furthermore, the presence of various renal structures, including tubules, and circular formations resembling glomeruli, suggests the recellularization of diverse renal cells forming different renal structures. The histological evaluation revealed a robust presence of cells distributed throughout the matrix, indicating a high recellularization percentage and the formation of colonies by WKPCs. H&E staining was performed on various sections, including the superior pole, superior half, inferior half, and inferior pole, encompassing the parenchyma, collecting ducts, and medulla. The sampling of both cortex and medulla from different sections validated the successful reach of cells to the organ's periphery. Cells exhibited alignment within tubular structures, closely resembling a native kidney, in the superior half. Furthermore, in the inferior half, there is evidence of cells recovering the wall of blood vessels. The presence of diverse cell types indicated the establishment of different cell populations crucial for functional kidney regeneration. Notably, interconnected cell networks were observed within the scaffold, indicating cell-to-cell communication and potential migration within the matrix. The image displayed a well-organized arrangement of cells, with varying cell densities across different regions. There were no signs of cell aggregation. It was observed circular structures suggesting the repopulation of glomeruli in the cortex, especially in the superior and the inferior pole (Fig. 4 ). The higher number of RBC observed could potentially obstruct the microarchitecture of the organ, posing a challenge for cell migration. Nevertheless, the protocol demonstrated effective cell perfusion in the cortex and medulla. Identification of primary porcine renal cells in Recellularized pig kidney Fluorescence microscopy analysis revealed that the glomeruli-like structures in the seeded kidney were densely populated with podocytes in the cortex across all sampled sections, highlighting their successful integration. Endothelial cells expressing CD31 were predominately aligned within vascular structures in both the medulla and the cortex, with enhanced expression in the half sections of the kidney. Furthermore, positive staining for AQP4 indicated site-specific adhesion of the extracellular matrix by epithelial cells in the tubular regions of the medulla, particularly in the collecting ducts. Vimentin expression was detected in the renal tubules, showing a high abundance in all sections. Additionally, the presence of aquaporin 1-expressing epithelial cells formed structures resembling the native proximal tubular epithelium observed in the cortex, primarily concentrated in the poles of the organ. These findings suggest that the seeded cells exhibit site-specific localization and the ability to form structures resembling the native renal components (Fig. 5 a-e). Ki-67 staining demonstrated the presence of 58.1 ± 17.2% brown-labelled proliferating cells distributed throughout the recellularized kidney, indicating active cell division of the WKPC inside the organ. This suggests that the recellularization process has successfully supported cell proliferation within the tissue scaffold (Fig. 5 b). In contrast, the Caspase-3 staining revealed 14.22 ± 4.46% of brown-labelled apoptotic cells in the recellularized pig kidney, indicating minimal cell death within the tissue. This indicates a favourable environment for cell survival and suggests that the recellularization protocol provides enough oxygen and nutrients to support the milieu for the maintenance and viability of the introduced WKPC (Fig. 5 f). The number of cells present in the culture media accounted for less than 1% of the initially seeded cells, indicating the recellularization process was successful since the ECM retained 99% of the cells. Although LDH values gradually increased during the reseeding process, there was not a significant production of LDH, consistently remaining below 16 U/L. The normal level of LDH in human blood is bellow 280 U/L, demonstrating that 16U/L does not express signs of cytotoxicity. The histological analysis and the assessment of LDH levels mutually reinforce each other, providing robust evidence for the successful retention and viability of the seeded cells within the recellularized kidney. The protocol of recellularization resulted in low tissue damage, low cell death and cell migration to the periphery of the organ within the recellularized kidney (Fig. 5 g). Discussion The successful reseeding of entire pig kidneys represents a significant advancement in the fields of tissue engineering and transplantation. Given the intricate structure of the kidney, which includes a complex network of capillaries and thousands of nephrons with intricate functional structures, the decellularization process is the most suitable method for bioengineering a functional kidney. To further advance kidney bioengineering, it is imperative to establish an optimal protocol for recellularizing kidneys of clinically relevant sizes The success of the decellularization process was confirmed by the DNA content, which measured below 50 ng of DNA per milligram of tissue, meeting the criteria for organ decellularization. Preservation of the microarchitecture, including the integrity of the glomerular loops, as well as the retention of proteins within the extracellular matrix (ECM), further validated the efficacy of the decellularization process 7 . Zambon et al. 33 demonstrated that a combination of SDS and Triton X-100 is the optimal decellularization methodology, achieving comparable macroscopic and microscopic results while significantly reducing perfusion time from 72 to 45 hours. This modification minimizes ECM damage while ensuring complete cell removal. It's worth noting that Sullivan et al. decellularized pig kidneys for 36 hours using only 0.5% SDS, which might result in structural protein and growth factor damage. Zambon et al. 13 further evaluated this method and observed ECM damage when perfusing with 0.5% SDS, as confirmed by scanning electron microscopy (SEM). In contrast, Tan 34 found that decellularizing a rat kidney with 0.1% SDS and 1X Triton did not damage the ECM, as evidenced by SEM analysis. Additionally, canine and pig kidneys were decellularized in 8 and 12 hours, respectively, using SDS 0.5% perfusion and cryoablation. However, this process did cause damage to the overall ECM structure 35,36 . Histological analyses have consistently shown the preservation of vital ECM components such as collagen, laminin, fibronectin, elastin, and glycosaminoglycans (GAGs) after decellularization using 0.1% SDS and Triton X-100 7, 23, 37, 38 . Nevertheless, there has been a lack of a proteomic profile of the ECM post-decellularization of pig kidneys. Leuning et al. 39 reported a proteomic analysis for rat and human kidneys, obtaining results similar to our findings in porcine decellularized kidneys. Notably, our study revealed a higher presence of growth factors, particularly VEGF and FGF, which are crucial for cell adherence 24 . This may be attributed to the decellularization protocol using Triton/SDS, as Caralt et al. demonstrated good retention of FGF and VEGF in the matrix after decellularizing a rat kidney 37 . In the proteomic analysis of decellularized pig kidneys, we observed an increase in the relative concentration of elastin and certain collagens, including collagen IV, collagen V, and collagen I, which are primary constituents of the ECM. This finding contradicts the results reported by Hussein et al. 40 , who observed a significant reduction in elastin but aligns with the statement that collagen is preserved in a decellularized pig kidney scaffold. Additionally, the level of VEGF retained in the decellularized kidney was 64%, compared to the 38% reported by Hussein et al. 40 . Similarly, these researchers reported the retention of 44% for FGF, whereas, in our study, there was a 2.98-fold increase in the relative concentration of this growth factor 41 . These findings support the preservation of critical ECM components, which play a pivotal role in the recellularization process by promoting cell migration and enhancing cell viability. Preserving the growth factors offers the opportunity to guide the phenotypic specification of cells toward their original location-specific types 41 . It was found in this study that combining perfusion and diffusion techniques enabled better preservation of cell viability. Antegrade perfusion supplies cells to the vasculature and at the glomerular level, while retrograde perfusion repopulates the collecting system and the tubular lumen 23 . Negative pressure has been employed to enhance cell migration and extravasation. Previous studies have reported the benefits of using negative pressure during cell seeding, when regulated with controlled time and pressure, it does not damage the extracellular matrix (ECM) and promotes a more uniform cell distribution 17,39,42,43 . Poornejad et al. 43 specifically demonstrated a significant improvement in cell migration from the tubules to the cortex using negative pressure (-40mmHg) during ureteral perfusion, which aligns with the results obtained by our group. We can conclude that − 17inHg yielded more favorable results in terms of recellularization efficiency and distribution, indicating its potential as a key factor in optimizing the recellularization process. It is worth noting that our protocol differed from other studies by focusing on whole organ recellularization, perfusing various cell types through the vein, artery, and ureter, resulting in better outcomes with a higher recellularization percentage. This approach successfully demonstrated the presence of endothelial cells in the blood vessels, epithelial cells in the tubules, and podocytes in glomerular-like structures. We successfully isolated, cultured, and expanded primary porcine renal cells, which exhibited the expression of renal cell markers. These findings are consistent with those of Abolbashari et al. 44 , who also demonstrated the presence of AQP1, AQP4, and Podocin in cultured primary porcine renal cells. However, the percentages obtained during flow cytometry analysis differed between the two studies. The WKPC cells showed lower expression of AQP1 (65.3% vs. 72%) but higher expression of AQP4 and Podocin (69% vs. 20% and 13.7% vs. 2%, respectively). This indicates a heterogeneous population of vital kidney cells expressing markers crucial for the bioengineering of a functional kidney. Moreover, the researchers demonstrated that primary porcine renal cells maintain renal cell functions, such as sodium uptake, hydrolase activity, and erythropoietin production. Consequently, assessing the in vitro functionality of the whole pig kidney recellularization should be a focus of future trials. It is worth mentioning that for successful recellularization, early passages of the cells should be utilized to preserve the majority of the cells. Flow cytometry and RT-qPCR analysis revealed that, over passages, the number of cells expressing Vimentin surpassed the desired population. Furthermore, optimizing the culture media is necessary to maximize the preservation of endothelial cells and monocytes, as these cell types showed a higher decrease in abundance over passages. Abolbashari et al. 44 developed a methodology for the formation of renal tubular structures; however, their seeding method involved cell injection, which may result in ECM damage and inefficient repopulation of the whole organ. In contrast, the use of perfusion allowed for a uniform distribution and migration of cells throughout the entire recellularized kidney. We observed a significantly higher number of cells within the recellularized kidney at 12 days of culture, indicating a higher recellularization percentage and increased formation of renal structures, including tubular-like and glomerular-like formations. Furthermore, the presence of cells expressing AQP1 and AQP4 in the appropriate structures further supports the hypothesis that ECM components and distribution facilitate the migration of cells to their respective locations. Regarding cell viability, the analysis conducted through ki-67 and caspase-3 staining supported the effectiveness of the recellularization approach in promoting cell proliferation and reducing cell death, crucial factors for the successful development of functional renal tissue. The 54.1% proliferation rate within the recellularized kidney significantly surpasses the findings of Leuning et al. 39 , who reported a proliferation rate of only 7.8%. Additionally, more stained cells were observed compared to the studies conducted by Hussein et al. 40 and Abolbashari et al. 44 . Conversely, similar results were obtained when compared to Remuzzi et al.'s 42 findings in rat kidney recellularization. Bonandrini et al. 18 reported higher proliferation using PCNA in porcine kidney recellularization after 72 hours in culture. Concerning cell death during pig kidney reseeding, the percentage of cell death on days 3, 7, 14, and 28 was lower in Abolbashari et al. 33 study compared to the findings in our recellularization process, where 16.72% expressed caspase-3. This coincides with the results reported by Bonandrini et al. 18 using embryonic stem cells. Although the percentage of cells expressing caspase-3 is not as low as in other researchers' findings, it still falls within an acceptable range. However, addressing this issue and further reducing cell death should be a focus of future investigations. During the optimization process, it was observed that oxygenating the media through agitation was less effective than desired. Analysis of the culture media revealed poor retention and diffusive delivery of oxygen due to the low oxygen affinity of the culture media. Additionally, the organ lacked the constant gas exchange that occurs in 2D cultures, limiting effective oxygen transfer. To overcome these challenges, a natural oxygen carrier, hemoglobin found in red blood cells (RBCs), was utilized. The use of erythrocytes allowed for efficient oxygen delivery to the cells without relying on bubble generation and rupture, thus avoiding potential cell damage caused by shear forces. This approach also eliminated the limitations associated with gas liberation during agitation. The increased presence of RBCs resulting from this approach may lead to a higher repopulation of RBCs, which could potentially obstruct the microarchitecture of the organ and hinder cell migration. However, this test demonstrated that the method used is helpful to perfuse cells along different sections of the kidney since we can detect a high number of RBCs. Nonetheless, we continue to observe the formation of circular structures along the ECM. To further optimize the protocol, it is necessary to increase the amount of perfusing WKPC by at least eightfold and explore using different oxygen carriers 44 . In the context of transplanting bio-engineered kidneys, it is understood that porcine primary renal cells may not be suitable for clinical applications. However, this study served as a proof-of-principle, aiming to optimize the recellularization technique. It was demonstrated that the use of WKPCs showed promising characteristics, particularly their rapid proliferation rate, making them potential candidates for recellularization when a large number of cells are required and for further evaluation of in vivo functionality of the bioengineered organs. Notably, this study successfully obtained a significant number of cells from a small amount of tissue. From just 5.5 mg of tissue, approximately 16010^6 cells were obtained. Extrapolating these findings to the whole tissue, it can be estimated that approximately 3.9810^9 (three billion nine hundred eighty-five million) cells could be obtained. These results align with previous findings reported in the literature 44 . Conclusion In conclusion, the increasing incidence of End-Stage Renal Disease (ESRD) exacerbates the shortage of available organs for transplantation. The limitations of dialysis emphasize the urgent need for advancements in organ bioengineering. Bioengineered kidneys represent a promising solution to bridge the gap between organ supply and demand, ultimately enhancing the quality of life for patients in need. Efficient decellularization of clinically relevant-sized kidneys while preserving the structural and compositional integrity of the extracellular matrix (ECM) is a critical milestone in the development of bioengineered organs. This study has demonstrated that the use of perfusion, negative pressure, nutrient immersion, and oxygenation significantly enhances the recellularization process, promotes cell viability, and facilitates the formation of functional renal structures. Future research endeavors should focus on optimizing the culture conditions and automating key processes to make these bioengineered kidneys suitable for clinical applications, thus laying the groundwork for their potential use in human transplantation. Ultimately, this ground-breaking research opens up new avenues for exploring and developing regenerative therapies, fundamentally transforming the landscape of organ transplantation and positively impacting the lives of countless individuals in need of life-saving treatments. Declarations Acknowledgments This study received no funding. We would like to express our gratitude to Dr. Dr Lange and Dr. David Harriman for his insights during the planning of the experiments. Author Contributions ACLB, AIS and CICM conceived the study and designed the experiments. ACLB and CUMA carried out the organ harvesting, cell culture, immunofluorescence, and flow cytometry. ACLB was in charge of the decellularization and recellularization. ACLB and CUMA analyzed the data. ACLB, CCM and AIS wrote the manuscript. All the authors read and approved the submitted manuscript. Competing interests All authors declare no financial or non-financial competing interests. Data availability The datasets used and/or analyzed during the current study available from the corresponding author on reasonable request. References Benjamin, O. & Lappin, S. L. End-Stage Renal Disease. in StatPearls (StatPearls Publishing, 2021). Canadian Institute for Health Information. Treatment of End-Stage Organ Failure in Canada, Canadian Organ Replacement Register, 2012 to 2021: End-Stage Kidney Disease and Kidney Transplants-Data Tables. Ottawa, ON. (2023). Manns, B., McKenzie, S. Q., Au, F., Gignac, P. M. & Geller, L. I. The Financial Impact of Advanced Kidney Disease on Canada Pension Plan and Private Disability Insurance Costs. Can J Kidney Health Dis 4 , (2017). Canadian Institute for Health Information. Annual statistics on organ replacement in Canada, 2011 to 2021[report]. Ottowa, ON: CIHI. https://www.cihi.ca/en/annual-statistics-on-organ-replacement-in-canada-2012-to-2021. (2022). Canadian Institute for Health Information. Treatment of End‐Stage Organ Failure in Canada, Canadian Organ Replacement Register, 2011 to 2020: End‐Stage Kidney Disease and Kidney Transplants. Ottowa, ON: CIHI https://www.cihi.ca/en/organ-replacement-in-canada-corr-annual-statistics-2020, supplemented with data collected by the Quebec Branch of The Kidney Foundation of Canada as provided by renal units. (2021). Narres, M. et al. The Incidence of End-Stage Renal Disease in the Diabetic (Compared to the Non-Diabetic) Population: A Systematic Review. PLoS One 11 , e0147329 (2016). Sullivan, D. C. et al. Decellularization methods of porcine kidneys for whole organ engineering using a high-throughput system. Biomaterials 33 , 7756–7764 (2012). Trends in end-stage kidney disease in Canada, 2020 — Infographic | CIHI. https://www.cihi.ca/en/trends-in-end-stage-kidney-disease-in-canada-2020-infographic. Canadian Institute for Health Information. 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Kriz, W. & Kaissling, B. Structural Organization of the Mammalian Kidney. Seldin and Giebisch’s The Kidney 1 , 479–563 (2008). Rowan, C. J., Sheybani-Deloui, S. & Rosenblum, N. D. Origin and Function of the Renal Stroma in Health and Disease. Results Probl Cell Differ 60 , 205–229 (2017). Kidd, M. E., Shumaker, D. K. & Ridge, K. M. The Role of Vimentin Intermediate Filaments in the Progression of Lung Cancer. Am J Respir Cell Mol Biol 50 , 1–6 (2014). Bombelli, S. et al. PKHhigh/CD133+/CD24− Renal Stem-Like Cells Isolated from Human Nephrospheres Exhibit In Vitro Multipotency. Cells 9 , 1805 (2020). Guo, Y. et al. Podocyte-Specific Induction of Krüppel-Like Factor 15 Restores Differentiation Markers and Attenuates Kidney Injury in Proteinuric Kidney Disease. J Am Soc Nephrol 29 , 2529–2545 (2018). Kapellos, T. S. et al. Human Monocyte Subsets and Phenotypes in Major Chronic Inflammatory Diseases. Frontiers in Immunology 10 , (2019). Starzonek, C. et al. Enrichment of Human Dermal Stem Cells from Primary Cell Cultures through the Elimination of Fibroblasts. Cells 12 , 949 (2023). Klemmt, P. A. B., Carver, J. G., Kennedy, S. H., Koninckx, P. R. & Mardon, H. J. Stromal cells from endometriotic lesions and endometrium from women with endometriosis have reduced decidualization capacity. Fertil Steril 85 , 564–572 (2006). Jp, Z. et al. Comparative analysis of two porcine kidney decellularization methods for maintenance of functional vascular architectures. Acta biomaterialia 75 , (2018). Tan, Z. Organ decellularization used as a novel approach to engineer three-dimensional urogenital tumor models. (University of British Columbia, 2019). doi:10.14288/1.0379729. Tajima, K. et al. Decellularization of canine kidney for three-dimensional organ regeneration. Vet World 13 , 452–457 (2020). Poornejad, N. et al. Efficient decellularization of whole porcine kidneys improves reseeded cell behavior. Biomed. Mater. 11 , 025003 (2016). Caralt, M. et al. Optimization and critical evaluation of decellularization strategies to develop renal extracellular matrix scaffolds as biological templates for organ engineering and transplantation. Am J Transplant 15 , 64–75 (2015). Uzarski, J. S. et al. Sustained in vivo perfusion of a re-endothelialized tissue engineered kidney graft in a human-scale animal model. Frontiers in Bioengineering and Biotechnology 11 , (2023). Leuning, D. G. et al. Vascular bioengineering of scaffolds derived from human discarded transplant kidneys using human pluripotent stem cell–derived endothelium. Am J Transplant 19 , 1328–1343 (2019). Hussein, K. H. et al. Biocompatibility and hemocompatibility of efficiently decellularized whole porcine kidney for tissue engineering. Journal of Biomedical Materials Research Part A 106 , 2034–2047 (2018). de Haan, M. J. A., Witjas, F. M. R., Engelse, M. A. & Rabelink, T. J. Have we hit a wall with whole kidney decellularization and recellularization: A review. Current Opinion in Biomedical Engineering 20 , 100335 (2021). Remuzzi, A. et al. Experimental Evaluation of Kidney Regeneration by Organ Scaffold Recellularization. Sci Rep 7 , 43502 (2017). Poornejad, N. et al. Re-epithelialization of whole porcine kidneys with renal epithelial cells. J Tissue Eng 8 , 2041731417718809 (2017). Abolbashari, M. et al. Repopulation of porcine kidney scaffold using porcine primary renal cells. Acta Biomater 29 , 52–61 (2016). Additional Declarations No competing interests reported. Supplementary Files Supplementarydata.docx Cite Share Download PDF Status: Published Journal Publication published 09 Oct, 2024 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 04 Apr, 2024 Reviews received at journal 03 Apr, 2024 Reviewers agreed at journal 24 Mar, 2024 Reviews received at journal 22 Mar, 2024 Reviewers agreed at journal 11 Mar, 2024 Reviewers invited by journal 11 Mar, 2024 Editor assigned by journal 11 Mar, 2024 Editor invited by journal 10 Mar, 2024 Submission checks completed at journal 10 Mar, 2024 First submitted to journal 09 Feb, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3943939","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":278648274,"identity":"bbac0e80-6eb9-4a96-83c6-10b9086f2094","order_by":0,"name":"Ana C. Luque-Badillo","email":"","orcid":"","institution":"University of British Columbia","correspondingAuthor":false,"prefix":"","firstName":"Ana","middleName":"C.","lastName":"Luque-Badillo","suffix":""},{"id":278648276,"identity":"eca11f64-c70a-4b6e-94e4-675b6b2e8b31","order_by":1,"name":"Cesar U. Monjaras-Avila","email":"","orcid":"","institution":"University of British Columbia","correspondingAuthor":false,"prefix":"","firstName":"Cesar","middleName":"U.","lastName":"Monjaras-Avila","suffix":""},{"id":278648278,"identity":"31b636c2-c826-42b2-a840-0937b31d3105","order_by":2,"name":"Hans Adomat","email":"","orcid":"","institution":"University of British Columbia","correspondingAuthor":false,"prefix":"","firstName":"Hans","middleName":"","lastName":"Adomat","suffix":""},{"id":278648279,"identity":"198dddd3-b2cd-48de-b055-ef4cb2df3458","order_by":3,"name":"Alan So","email":"","orcid":"","institution":"University of British Columbia","correspondingAuthor":false,"prefix":"","firstName":"Alan","middleName":"","lastName":"So","suffix":""},{"id":278648282,"identity":"d0322018-2c24-45bd-bd30-eac00524d127","order_by":4,"name":"Claudia Chavez-Munoz","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABEklEQVRIie3QMUvDQBTA8RcCveVB1lcE+xUuBAId9Iu4BALtkoOK4OKgEEiX4hyxHyJ+g8BBuzkfmEERMnUIFKRDQM+AIPaarg73h7vhcT/uOACb7T/G9EKuN/o9baA8TFwTcfKjBP4QF3uIl7o1bWbn4D2mNTUtXPCXVG7PsmoETL6aCMlBOFzyGKhahcMcQRTVKgpEVvt3OOEmwl0IT1DvnKIgQPoUhUp4LDIZ6aceIOxDk1tNptug5dAROe4Ia8wEv2+RmiT+O0Qd8VOnI2i8hSRejZd8jaSSy7dFCeJBTWJn8Vz7GSYz44/N509q096cevm0KHctiHsVy2Z3XY08ti5M5CfcHw36zttsNputty9DYlogGS8x9wAAAABJRU5ErkJggg==","orcid":"","institution":"University of British Columbia","correspondingAuthor":true,"prefix":"","firstName":"Claudia","middleName":"","lastName":"Chavez-Munoz","suffix":""}],"badges":[],"createdAt":"2024-02-09 19:14:41","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-3943939/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-3943939/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1038/s41598-024-74543-w","type":"published","date":"2024-10-09T15:57:42+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":52620201,"identity":"b283c91c-c517-473d-ad82-19baebff04f0","added_by":"auto","created_at":"2024-03-13 16:44:04","extension":"jpeg","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":1010047,"visible":true,"origin":"","legend":"\u003cp\u003e\u003cstrong\u003eEvaluation of decellularized pig kidney\u003c/strong\u003e. (a) H\u0026amp;E showed a complete cell removal in the renal medulla (b) microscopic evaluation of the preservation of the cortex of a pig kidney. The arrows point to the preservation of the glomeruli. Slides scanned at 40X (Scale bar 100mm \u0026amp; 200 mm, respectively). (c) DNA quantification of pig native and decellularized kidneys (Native 1113.17 ng/ml ± 100.02 ng/ml, Decellularized 12.24 ng/ml ± 4.07 ng/ml, n=5). The DNA content was reduced 99% in decellularized pig kidneys. (d) Macroscopic progression of a decellularized pig kidney, after 45h of decellularizing reagents perfusion through the renal artery, and renal vein, we obtained a complete cell removal.\u003c/p\u003e","description":"","filename":"floatimage1.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-3943939/v1/5fc10913a8382d633630ccd8.jpeg"},{"id":52621077,"identity":"26179efa-5ef5-42df-9722-4c3722df86bf","added_by":"auto","created_at":"2024-03-13 16:52:04","extension":"jpeg","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":771317,"visible":true,"origin":"","legend":"\u003cp\u003eEvaluation of the WKPC. (a) qPCR of whole kidney pig cells. The analysis showed the presence of CDH1 and Vimentin expression increasing with passages, as depicted in the top graph. (b) The markers AQP1, AQP4, CD14, CD31 and Podocin, were also present but exhibited a decreasing level of expression with the passages (n=4). After the dissociation and culture of primary porcine renal cells it was found a heterogenous population with cells expressing kidney cell markers such as AQP1(c), and epithelial cells CDH1 (d), AQP4 (e), endothelial cells (f) and stroma cells with podocytes (g). The analysis was performed at 20X magnification, with a scale bar of 20µm.\u003c/p\u003e","description":"","filename":"floatimage2.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-3943939/v1/930ced09e1c4e57b4c14d9ad.jpeg"},{"id":52620203,"identity":"7625a86b-ccb1-4b5d-af16-efd230bf8edb","added_by":"auto","created_at":"2024-03-13 16:44:04","extension":"jpeg","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":1597420,"visible":true,"origin":"","legend":"\u003cp\u003eRecellularized pig kidney. (a) H\u0026amp;E staining of the reseeded pig kidney revealed a significant increase in cell presence and the formation of glomeruli-like structures compared to the decellularized kidney following 12 days of culture. The slide was scanned at 40X magnification. (b) Macroscopic evaluation of recellularized pig kidney. The recellularization process involved the perfusion of culture media, WKPC, and RBC through the renal artery, renal vein, and ureter for a culture time of 12 days.\u003c/p\u003e","description":"","filename":"floatimage3.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-3943939/v1/03cdf214b246816089b2749b.jpeg"},{"id":52620205,"identity":"0eae2180-6016-4fbe-9f69-ed5d58203a48","added_by":"auto","created_at":"2024-03-13 16:44:04","extension":"jpeg","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":1387425,"visible":true,"origin":"","legend":"\u003cp\u003eHistological evaluation of a recellularized pig kidney. Different sections of the organ were analyzed, including the superior pole, superior half, inferior half, and inferior pole. Sampling zones encompassed the parenchyma, collecting ducts, and the medulla. The distribution of cells was found to be uniform throughout the entire tissue, demonstrating a high percentage of cell reseeding and migration towards the periphery of the organ. The slide was scanned at 40X magnification.\u003c/p\u003e","description":"","filename":"floatimage4.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-3943939/v1/cdfd06ac4fab4d002e793b6d.jpeg"},{"id":52621075,"identity":"eb0e58a0-7929-4893-9464-fba4f3226753","added_by":"auto","created_at":"2024-03-13 16:52:04","extension":"jpeg","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":930345,"visible":true,"origin":"","legend":"\u003cp\u003eIF staining of the reseeded pig kidney illustrates the presence of cell markers in various kidney sections. Endothelial cells expressing CD31 (a) as well as proximal tubule markers aquaporin 1 (b), vimentin (c) and aquaporin 4 were detected in the collecting ducts (d) of the recellularized kidney. Notably, podocin-positive cells are observed, forming glomeruli-like structures (e). The IF analysis was conducted at 20X magnification, with a scale bar of 20µm. (f) The Ki-67 staining showed the presence of 58.1±17.2% positive cells, indicating active proliferation. Conversely, Caspase-3 staining exhibited 14.22±4.46% positive cells suggesting low levels of apoptosis and cell death after recellularization. (g) The LDH values exhibited variation throughout the recellularization process, consistently measuring above 15 mU/mL. The media perfused through the recellularized kidney demonstrated low cytotoxicity, suggesting that the cells perfused remained viable within the organ.\u003c/p\u003e","description":"","filename":"floatimage5.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-3943939/v1/b50b2cfed2c46d3a383fd379.jpeg"},{"id":66597192,"identity":"ca1f81af-b682-4ef3-afc9-7d7cc7137d0e","added_by":"auto","created_at":"2024-10-14 16:08:14","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":7488836,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-3943939/v1/cc89caac-d6ba-4b43-aeda-2f67cdd218d6.pdf"},{"id":52620202,"identity":"8ca3c579-d1ca-4352-8a59-64dd01b027a8","added_by":"auto","created_at":"2024-03-13 16:44:04","extension":"docx","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":1199679,"visible":true,"origin":"","legend":"","description":"","filename":"Supplementarydata.docx","url":"https://assets-eu.researchsquare.com/files/rs-3943939/v1/5663c4fba5900ba88ab72aee.docx"}],"financialInterests":"No competing interests reported.","formattedTitle":"Evaluating different methods for kidney recellularization","fulltext":[{"header":"Introduction","content":"\u003cp\u003eEnd-stage renal disease (ESRD) is a condition in which the kidneys lose their ability to filter toxins and excess fluids from the bloodstream, leading to a dangerous accumulation of waste products and electrolytes in the body. This not only reduces the patient's quality of life but also results in premature mortality 1. In 2021, the prevalence of ESRD in Canada reached 48,000 cases, marking a 24% increase over the past decade. This surge in cases places a significant annual healthcare burden of 40\u0026nbsp;billion dollars on the system 2\u0026ndash;4. The prevalence of ESRD is expected to rise further due to its strong association with chronic diseases like diabetes mellitus and hypertension, both of which are prevalent in the aging Canadian population 5\u0026ndash;7.\u003c/p\u003e \u003cp\u003eThere are two primary treatments for ESRD: dialysis and kidney transplant 4. Although kidney transplant is considered the optimal treatment, most patients must undergo dialysis initially. The demand for chronic dialysis has nearly doubled over the past two decades, costing the Canadian healthcare system over \u003cspan\u003e$\u003c/span\u003e100,000 per patient annually 3,8. Kidney transplant remains the most cost-effective and survival-enhancing option, with a 41% increase in transplant cases in the last decade \u003csub\u003e10\u003c/sub\u003e. However, the shortage of available organs is a critical issue, with 77% of individuals on the organ waitlist in Canada awaiting a kidney, with an average wait time of 3 years and ten months 4,10,11.\u003c/p\u003e \u003cp\u003eTo address this organ shortage problem, tissue engineering emerges as a potential solution. Tissue engineering combines engineering principles with cell biology to develop biological substitutes capable of restoring or enhancing tissue and organ function. One technique in tissue engineering is decellularization, which removes cellular components from tissues or organs while preserving the extracellular matrix (ECM). The ECM provides structural support, native vascular networks, and bioactive molecules, enhancing cell recellularization and differentiation 12,13. Recellularization involves reseeding the decellularized organ with cells. While this process can be performed via perfusion through the renal artery, renal vein, or ureter, there are challenges such as oxygenating cells, recirculating media to preserve nutrients, and ensuring cell viability and migration. Current methods have a low reseeding success rate and difficulty in forming functional renal structures 14\u0026ndash;18.\u003c/p\u003e \u003cp\u003eThis study aims to improve kidney recellularization methods using primary porcine renal cells as a proof of principle. The research demonstrates that perfusion-based decellularization and recellularization can achieve a high cell density in the ECM, low cytotoxicity levels, and the presence of kidney cell markers throughout the kidney. This research could pave the way for similar techniques in human kidneys, offering hope for regenerative medicine in ESRD treatment and improving organ availability for transplantation.\u003c/p\u003e"},{"header":"Materials and Methods","content":"\u003cp\u003eThe reporting of this study meets the ARRIVE essential 10 criteria.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eExperimental Animals and Tissue Collection\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eOur team operates under an organ retrieval animal protocol (A22-0119) sanctioned by the Animal Care Committee of the University of British Columbia (UBC), confirming that all experiments were performed in strict accordance with guidelines and regulations established by the Institutional Animal Care and Use Committee (IACUC) of the University of British Columbia. This protocol ensures compliance with ethical standards and animal welfare principles while facilitating collaboration with existing research endeavors.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eAll kidneys utilized in this study were sourced from adult female Yorkshire pigs (n=19). No animals were specifically procured for sacrifice; rather, the organs were retrieved from pigs involved in other ongoing research protocols upon the conclusion of their respective experiments. Prior to kidney retrieval, pigs were anesthetized by injecting Ketamine S (20mg/kg i.m.), infusing 500ml of lactated Ringer’s solution (1-4 ml/kg/h to prevent hypovolemia, followed by propofol (20-30 mg i.v. in increments). Pigs are then intubated with an endotracheal tube (5.5-6 mm internal diameter). Maintenance of anesthesia is performed by morphine (20 mg i.v.) and propagated by an infusion of propofol (6-8 ml/kg/h). For euthanasia, we inject morphine (1mg/kg i.v.) followed 10 min later by propofol (10mg/kg i.v), rocuronium (1mg/kg i.v.) and subsequent injection of potassium chloride (40mmol) unless otherwise specified by approved animal research protocols.\u0026nbsp;Prior to euthanizing the pigs, 50ml of 10% 1000 USP units/mL sodium heparin (C504036 Fresenius Kabi) was perfused in bolus via IV. Both the renal artery and the renal vein were clamped before removing one kidney, leaving the blood vessels intact for cannulation. The other kidney was removed with blood vessels because it was used for tissue dissociation and cell retrieval. The fresh kidneys were transported in PBS and ice to the biosafety cabinet SterilGard III (GMI).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eDecellularization\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eFollowing tissue collection, the kidneys underwent a thorough cleaning process. They were immersed three times for 3 seconds each in 300 mL of sterile ddH2O containing 0.1% hydrogen peroxide (product code 033323 from Fisher Scientific). Subsequently, the organs were rinsed with sterile PBS. To facilitate decellularization, catheterization of the renal vein and artery was performed using a sterile angiocath connected to an intravenous (IV) line (BAX2C8401 from Baxter). Male-to-male luer lock adapters were inserted at both ends of the IV line, and 2-0 silk sutures (J\u0026amp;J-685G Ethicon) were used to secure the tubes in place.\u003c/p\u003e\n\u003cp\u003eDecellularization was achieved through perfusion cycles that alternated between the renal artery and renal vein using a sterilized in vitro bioreactor treated with ethylene oxide. Male Luer lock adapters were connected to the bioreactor valves, which, in turn, were linked to a programmable peristaltic pump (BS-900 from Braintree Scientific Inc.) through a 3/16\" ID hose. Seven different methods were explored to optimize kidney decellularization, and the details of each method and their respective results can be found in the supplemental material (Appendix 1).\u003c/p\u003e\n\u003cp\u003eThe optimized decellularization protocol (method g) involved the following steps:\u003c/p\u003e\n\u003col\u003e\n \u003cli\u003ePerfusion of sterile ddH2O at 10 ml/min for 30 minutes, followed by an increase to 25 ml/min for another 30 minutes.\u003c/li\u003e\n \u003cli\u003eTwo cycles of detergents: first, 1% Triton 100X (M143 from VWR) at 25 ml/min for 120 minutes and then at 10 ml/min overnight for 840 minutes.\u003c/li\u003e\n \u003cli\u003ePerfusing 0.1% SDS (BP1311-1 from Fisher Bioreagents) at 25 ml/min for 360 minutes, totaling 45 hours of decellularization. This process took place in a bioreactor with dimensions of 30 cm in height and 25.4 cm in diameter.\u003c/li\u003e\n \u003cli\u003eConditioning the matrix by perfusing sterile ddH2O at 10 ml/min for one week.\u003c/li\u003e\n \u003cli\u003eA final wash with 1,800 mL of sterile water containing 5% antibiotics and antimycotics 100X (A/A) (#15240062 Gibco, NY USA).\u003c/li\u003e\n\u003c/ol\u003e\n\u003cp\u003eDecellularized organs were preserved while awaiting reseeding, using HBSS (product #14025092, Gibco) buffer with 1% A/A (Gibco) at 4°C.\u003c/p\u003e\n\u003cp\u003eTo validate the decellularization process, five sections from specific locations of the decellularized kidney and five sections from the same locations of a native kidney were analyzed to quantify the remaining DNA. This analysis was conducted using the DNeasy Blood \u0026amp; Tissue Kit (product #69504, Qiagen, CA, USA), processing 20 mg of tissue and eluting in 80 μL of elution buffer.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eHistology\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eSections from the superior pole, inferior pole and close to the renal hilum were extracted in both the cortex and medulla regions to perform the histological analysis of the decellularized and recellularized kidneys. The tissue samples were fixed for 24 hours in 10% neutral buffered formalin (Scigen CA, USA). Afterwards, they were dehydrated in 70% ethanol and embedded in paraffin. Sections of 5um of thickness were mounted on slides deparaffinized in the oven at 60C for one hour and rehydrated in washes of xylene and ethanol to later\u0026nbsp;stain with hematoxylin and eosin (H\u0026amp;E). The slides were examined using SCN400 Slide Scanner (Leica Biosystems, IL, USA) and analyzed with Aperio Digital Whole Slide Scanner (Leica Biosystems).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eImmunohistochemistry\u0026nbsp;\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe samples of the recellularized organs were deparaffinized, rehydrated, antigen retrieved, permeabilized and blocked. Afterwards, the slides were incubated with 3% hydrogen peroxide (Sigma-Aldrich, MO, USA) for 10 minutesand washed with PBS. Followed by the incubation with Ki-67 rabbit monoclonal antibody (RM-9106, Thermo Scientific, MA, USA) or with Anti-Cleaved Capsase-3 (ab2302, Abcam, Ma, USA) for 90 minutes at room temperature. The samples were washed with PBS and incubated with Vectastain ELITE ABC-Peroxidase kit (Vector Laboratories, CA, USA) for 30 minutes and washed to incubate later with DAB for 5 minutes. Finally, the slides were washed with ddH\u003csub\u003e2\u003c/sub\u003eO and dehydrated by immersion in ethanol and xylene. The staining intensity and distribution of positive cells were calculated by analyzing 5 sections of the recellularized kidney, using the image analysis software QuPath to quantify cellular proliferation and apoptosis within the recellularized tissue.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eProteomics\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eSamples of decellularized and fresh native kidneys were processed for protein extraction. Both groups were minced rigorously and 200 mg of tissue with 200uL of Ripa (R0278 Sigma-Aldrich, MO, USA) and cOmplete™ Protease Inhibitor Cocktail (Sigma-Aldrich) were homogenized in Tissue Grinding tubes with ceramic beads (P000922-LYSK0-A.0, Bertin Instruments, Montignyle-Bretonneux, France) using the Precellys® homogenizer (Bertin Instruments). Afterwards, the supernatant was sonicated (FB705, Fisher Scientific) for milliseconds at 50 of amplitude and centrifuged at max rpm for 4 minutes. The peptide concentration was determined using the Quick Start Bradford Assay (5000205,\u0026nbsp;Bio Rad) according to the manufacturer's instructions and a microplate reader (Epoch, BioTek). The single-pot, solid-phase-enhanced sample-preparation (SP3) method was used for the extraction and processing of the proteins; 100ug of each of the samples of decellularized and native kidney extracts were reduced with 4uL of 200mM 1, 4-Dithiothreitol (DTT, 1019777700, Sigma-Aldrich, 10197777001, Darmstadt, Germany) then alkylated by addition of 4uL of 400mM iodoacetamide (IAA) (90034, Thermo Fisher, MA, USA).\u0026nbsp;SP3 protein clean up and digestion was performed using 1mg (20µl) SeraMag Speedbeads (GE Healthcare) per sample, adding acetonitrile to 70% then washed with 200ul 80% EtOH (x3), and finally suspending in 50mM HEPES pH 8, 2µg trypsin/LycC.\u0026nbsp;After removing the beads, the digests were acidified by the addition of 50µl 0.2% FA Finally,\u0026nbsp;the samples were desalted by solid phase extraction with C18 Sep-Pak cartridges (Waters), conditioned with 1ml acetonitrile/0.1%. \u0026nbsp;The eluate was dried using a centrifugal evaporator (CentriVap) and residue dissolved in a 20ul 0.1% FA.\u0026nbsp;Prepared samples were analysed using an Easy nLC 1200 connected to an Orbitrap Lumos The Easy nLC was set up with a PepMap RSLC C18 75µm x 50cm, 2µm column at 50⁰C using the following gradient: 0-5 min 2% ACN; 5-140min, 2-28% ACN; 140-150min, 28-40% ACN; 150-160min, 40-95% ACN; 160-170min, 95% ACN. \u0026nbsp;Column equilibration was 8µl and sample injects 2µl with 6µl loading volume with total run times about 3hrs. \u0026nbsp;The Easynano source was operated at 2400V and m/z 375-1500 orbitrap (OT) MS1 survey scan data collected at 120000 resolution (profile), RF lense 30%, AGC 4e5, 50ms max. \u0026nbsp;Data dependant MS2 data was collected with peptide monoisotopic selection, 5.0e4 threshold, charge states 2-7, 60 sec dynamic exclusion (10ppm), quad isolation m/z 0.8, HCD activation 30%, OT 15000 resolution (centroid), auto scan range with first mass m/z110, 5.0e4 target with max inject time of 100ms and cycle time of 3sec.\u003c/p\u003e\n\u003cp\u003eData was processed using Thermo Proteome Discoverer 2.5 with Sequest and the Sus Scrofa dataset (Sus Scrofa TaxID=9823, 2022-03-02 and Homo SapiensTaxID=9606) with the following parameters: precursor mass 350-5000 Da; enzyme, trypsin; missed cleavage, 2; min/max peptide length 6/144; precursor/fragment tolerance, 10ppm/0.02Da; all neutral losses; b,y ions only; static modification, carbamidomethyl; dynamic modifications, oxidation (K, M, P), protein terminus acetyl; Cn max 0.05; PSM, peptide and decoy DB target FDR’s, 0.01 strict, 0.05 relaxed, based on q-value. \u0026nbsp;Filtering was set for peptides at least medium confidence however only high confidence data was selected with final post process filtering with Discoverer. \u0026nbsp; Protein and Protein Group were exported to Excel for further sorting and analysis.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003ePrimary cell culture\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe cells used for recellularization were obtained from fresh kidneys of pigs undergoing euthanasia in our Animal facility under our animal protocol (A22-0119) approved by the University of British Columbia.\u0026nbsp;To obtain a population of cells labelled as whole kidney pig cells (WKPC) the organ was minced and weighted\u0026nbsp;∼5.5 mg was\u0026nbsp;dissociated with Collagenase A (10103586001, Sigma-Aldrich) at 2.25 mg/ml and Dispase II (17105-041, Gibco) 10U in a mixture of HBSS and DMEM an incubated at 37C for two hours gently shaking the mixture continuously. The mixture was filtered through a 70um and 40uM cell strainer and centrifuged to later be incubated 10 minutes with TrypLE (12605028, Gibco). The culture was monitored and expanded until passage five at\u0026nbsp;37\u0026nbsp;°C and 5% CO\u003csub\u003e2\u0026nbsp;\u003c/sub\u003e in 175cm flasks, using kidney mix media (KMM) supplemented with 5.1% of FBS (Hyclone, UT, USA) and 5% A/A, the composition consisted of one third of DMEM (11965092, Gibco), one third of REBM (CC-3190, Lonza, GA, USA) supplemented with 0.1% hEGF, 0.1% Insulin, 0.1% Hydrocortisone, 0.1% GA-1000, 0.1% Transferrin, 0.1% Triiodothyronine, 0.1% Epinephrine, and one-third of EGM (CC-3162, Lonza), supplemented with 0.04% Hydrocortisone, 0.4% \u0026nbsp; hFGF-B, 0.1% VEGF, 0.1% R3-IGF-1, 0.1% Ascorbic Acid, 0.1% hEGF, 0.1% GA-1000, 0.1% Heparin. The passage was done when the cells were at 80% confluency with 0.25% Trypsin-EDTA (#25200056, Gibco, MD, USA) for 10 minutes at 37°C was used for detachment and with later passages, a cell scrapper was needed. WKPC were frozen in liquid nitrogen with Bambanker (BB01, Cederlane) in cryovials for better preservation. The number of cells were determined with the Automated Cell Counter TC20 (Bio-Rad, WA, USA).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eFlow cytometry\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003ePrimary porcine renal cells were used with and without culture directly after cell dissociation. Expanded WKPC cells were detached using Cell Stripper (VWR, Cat# CA4500-668), a non-enzymatic reagent washed with PBC and resuspended in FACS buffer (1X PBS, 2.5mM EDTA, 2% FBS, 0.05% sodium aside). To analyze for viability, The Zombie NIR™ dye (423107, BioLegend, CA, USA) in a 1:100 dilution was added to the cells and incubated for 15 minutes. For blocking, the cells were incubated on ice with Fc block (564220, BD biosciences)\u0026nbsp;diluted in a1:300 for 5 minutes. For surface staining, the primary antibodies were added and incubated on ice, in the dark for 45 minutes. Due to the lack of antibodies for pig samples, anti-human antibodies were used with predicted reactivity with pig. The following primary antibodies were used: anti-human CD14-AF700 (301822, BioLegend), anti-mouse/human E-Cadherin-BV421 (147319, Bio Legend), anti-mouse/human AQP1-AF594 (bs-1506R-A594, Bioss USA, MA, USA), anti-mouse/human AQP4-APC-Cy7 (bs-0634R-APC-Cy7, Bioss USA), anti-mouse/human Podocin-PE (bs-6597R-PE, Bioss USA), anti-human CD31-APC (303115, BioLegend). After washing, the cells underwent intracellular protein staining, involving fixation, permeabilization, and incubation in the dark with Anti-Human Vimentin-AF488 (562338, BD Biosciences) for 15 minutes. Finally, the cells were filtered, resuspended in FACS buffer, and analyzed by FACS Canto II Flow Cytometry System (BD Bioscience). \u0026nbsp; The percentage of positively stained cell count was quantified using FlowJo_V10.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eQuantitative real-time PCR (RT-qPCR)\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eWKPC from every passage (2 x 10\u003csup\u003e6\u003c/sup\u003e cells) were washed with 100 uL of PBS and then stored in 100uL of RNA later at - 20°C. The total RNA was extracted with the RNeasy kit (Qiagen) following the manufacturer's instructions. The cDNA synthesis was performed using 1000ng of RNA and the High Capacity cDNA Reverse Transcription Kit (4368813 Applied Biosystems, CA, USA). The analysis of real time PCR (RT-PCR) was done QuantStudio 7Pro (Applied Biosystems) using PowerTrack™ SYBR™ Green Master Mix (4309155 applied biosystem). The reaction was carried out with an initial denaturation of 2 minutes followed by 40 cycles of denaturation of 95 for 5 seconds and elongation at 60 for 35 seconds. Using 300nM of gene-specific designed primers for cell kidney markers and GADPH as a housekeeping gene for normalization and quantifying gene expression which can been found in the supplemental material (Appendix 2).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eRecellularization\u0026nbsp;\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe tubbing, Luer lock adaptors and the bioreactor were sterilized using ethylene oxide. The decellularized organ was connected through the renal vein and the renal artery to the valves of the bioreactor employing the same IV lines that were used for decellularization, connecting them with Luer lock adapters to the valves of the bioreactor; the assembling, sterilization and cell perfusion was done on the biosafety cabinet. For the sterilization of the organ, ddH\u003csub\u003e2\u003c/sub\u003eO with 1% A/A was perfused through the renal artery and renal vein at 10ml/min using a peristaltic pump\u0026nbsp;(BS-900 Braintree Scientific Inc.), after the continuous perfusion 150ml\u0026nbsp;of ddH\u003csub\u003e2\u003c/sub\u003eO with 10% A/A were left inside the organ for 2 hours.\u003c/p\u003e\n\u003cp\u003eUsing a\u0026nbsp;syringe 40 ×\u0026nbsp;10\u003csup\u003e6\u003c/sup\u003e WKPC were infused into the renal artery resuspended in 45 ml of \u0026nbsp;DMEM 10% FBS and 1% A/A, 40 × 10\u003csup\u003e6\u003c/sup\u003e WKPC perfused through the renal vein and 20 × 10\u003csup\u003e6\u003c/sup\u003e WKPC\u0026nbsp;perfused through the ureter, previously catheterized, -17inHg of negative pressure was applied after 10 minutes in static state, only during the first day of cell perfusion. Five identical cycles of cell perfusion were done having a total of 500\u0026nbsp;× 10\u003csup\u003e6\u003c/sup\u003e WKPC.\u0026nbsp;DMEM 10% FBS and 1% A/A,\u0026nbsp;was perfused through the renal artery every 24 hours for four days for 1 hour at 10ml/min. At the end of the fourth day, the bioreactor was set up in continuous recirculation of supplemented DMEM at 10ml/min inside an incubator for 8 days, the media was changed every day and oxygenated by agitation for one hour daily. On the ninth day, oxygenation was provided using red blood cells (RBC) extracted isolated from human’s blood employing Ficoll-Paque PLUS (#17144003, Cytivia Life Sciences). The RBC are mixed with the media and perfused into the kidney with daily oxygenation by agitation. The media was stored everyday using\u0026nbsp;cOmplete™ Protease Inhibitor Cocktail (Roche, Germany) at -80C, later on it was\u0026nbsp;analyzed to count and collect the number of cells that flowed out of the kidney, obtaining the retention of cells following the methodology proposed by Hochman-Mendez \u003csub\u003e19\u003c/sub\u003e. On the twelfth day the kidney was sectioned and prepared for histology analysis.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eLDH Assay\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe media obtained after the recellularization was thawed and analyzed using the LDH-Glo™ Cytotoxicity Assay (Promega, WI, USA) according to the manufacturer's instructions, using 2μl of 10% Triton X-100 per 100μl of media as a negative control. The assay was done with 25uL of samples and 60 minutes of incubation time, and luminescence was recorded using a luminometer infinite m200 pro (Tecan, Switzerland)\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eImmunofluorescence\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eFor the analysis of cells, WKPC’s were cultured in\u0026nbsp;Nunc™ Lab-Tek™ Flask on Slide (170920,\u0026nbsp;Thermo Fisher, MA, USA)) and were allowed to grow for 72 hours, washed with 1X filtered PBS and fixed with\u0026nbsp;4% paraformaldehyde (Sigma-Aldrich, MO, USA).\u0026nbsp;Regarding the study of the recellularized kidney sections,\u0026nbsp;five slides of the\u0026nbsp;inferior pole with longitudinal\u0026nbsp;cut were deparaffinized, once rehydrated they were treated with\u0026nbsp;Diva Decloaker 10X (Cedarlane, ON, CA) for 30 mins in boiling water for the\u0026nbsp;antigen retrieval, then washed with\u0026nbsp;dH\u003csub\u003e2\u003c/sub\u003eO. Both Sections and cell’s slides were washed with PBS, permeabilized with 0.3% Triton X-100. incubated with Fc blocker and blocked for non-specific binding with 2.5% horse serum (Vector Laboratories, CA, USA) for 30 minutes.\u003c/p\u003e\n\u003cp\u003eSlides were stained with Mouse Anti-E-Cadherin at 1:500 (610181\u0026nbsp;BD Bioscience), mouse anti-Aquaporin-1 AT 1:400 (ab9566\u0026nbsp;), \u0026nbsp;mouse anti-CD31 at a1:100 (ab24590), rabbit anti-Vimentin at 1:200 (mAb 5741, rabbit anti-aquaporin-1 at 1:500(mAb59678\u0026nbsp;\u0026nbsp;), rabbit anti-podocin at 1:500 (ab216341) antibodies incubated overnight at 4 °C. Goat Anti-Mouse IgG (Alexa Fluor 569) and Goat Anti-Rabbit IgG (Alexa Fluor 488) \u0026nbsp;were used as secondary antibodies for staining at a 1:200 dilution for two hours in the dark, the slides were mounted with DAPI (Vector Laboratories, CA, USA) and imaged with confocal microscope at 20X and 60X magnifications (Olympus FV3000RS).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eStatistical Analysis\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eR studio (2021.09.0) was used to analyze the statistical difference between two groups using a paired t-test. It was considered statistically different when the\u0026nbsp;probability value \u003cem\u003eP\u003c/em\u003e\u0026lt;0.05. Data were presented as mean±S.D\u003c/p\u003e"},{"header":"Results","content":"\u003cp\u003e \u003cb\u003eDecellularization\u003c/b\u003e \u003c/p\u003e \u003cp\u003eThe primary objective was to minimize the exposure of the extracellular matrix (ECM) to detergents by adjusting the flow rates, rather than altering the duration of detergent exposure in each cycle. We successfully established a decellularization protocol for pig kidneys using perfusion, achieving complete decellularization within 45 hours (Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e). Macroscopic evaluation demonstrated effective cell removal through detergent perfusion via the renal vein and renal artery, resulting in partially transparent decellularized organs that retained their structures and vascular networks.\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003eConsistent with the macroscopic assessment, microscopic evaluation of various sections of the decellularized organs using H\u0026amp;E staining revealed complete cell removal in the renal medulla and cortex of pig kidneys. This preservation of the organ's microarchitecture indicated the production of a high-quality ECM suitable for recellularization. In the medulla, the characteristic honeycomb-like appearance was maintained (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003eA), while in the cortex, the loops corresponding to the glomerulus, a crucial kidney component, were preserved (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003eB).\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003eDouble-stranded DNA content was quantified and compared between decellularized kidneys (12.24 ng/\u0026micro;l\u0026thinsp;\u0026plusmn;\u0026thinsp;4.07 ng/\u0026micro;l) and native kidneys (1113.17 ng/\u0026micro;l\u0026thinsp;\u0026plusmn;\u0026thinsp;100.02 ng/\u0026micro;l), revealing a statistically significant difference with a p-value of \u0026lt;\u0026thinsp;0.0001 (1.578*10^-05). This difference signifies a reduction of over 99% in residual DNA in the decellularized pig kidneys, suggesting the elimination of the majority of cells and any remaining DNA (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003eC).\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003e \u003cb\u003eProteomic analysis of the extracellular matrix\u003c/b\u003e \u003c/p\u003e\u003c/div\u003e\u003c/p\u003e \u003cp\u003eOut of the 4,620 proteins identified in native pig kidneys, 3,162 proteins were found in the decellularized pig kidneys. A comparison between the decellularized and native kidneys revealed an overlap of 2,715 proteins, with 447 proteins uniquely identified in the decellularized tissue and 1,895 proteins exclusively detected in the native kidney. The absence of certain proteins in the decellularized kidney can be attributed to the removal of cellular components, including enzymes and organelle-specific proteins. It's important to note that the samples were derived from different kidneys.\u003c/p\u003e \u003cp\u003eTable\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e presents a total of 170 proteins, which include critical components of the extracellular matrix. Notably, the decellularized organ exhibited the presence of seven different types of growth factors. The number of peptides corresponds to the unique peptides identified per protein by the mass spectrometer, while coverage indicates the proportion of amino acids covered by the identified peptides relative to the total protein length. Abundance reflects the combined intensity of all analyzed peptides, serving as a marker for quantifying protein relative abundance in the organ \u003csub\u003e20,21\u003c/sub\u003e.\u003c/p\u003e \u003cp\u003eThe decellularized kidney displayed high concentrations of key proteins such as collagen IV, collagen V, fibronectin, elastin, glycoproteins, integrin, laminin, and fibrillin. These proteins play a critical role in establishing a functional scaffold, preserving cell viability, and facilitating cellular recruitment \u003csub\u003e12,22,23\u003c/sub\u003e. The fold rate indicates changes in relative protein concentration during the decellularization process. Due to the removal of cellular components, some proteins showed increased relative concentrations in the decellularized organ, as indicated by fold rates greater than 1.\u003c/p\u003e \u003cp\u003eNotably, certain growth factors exhibited higher relative concentrations in the decellularized kidney, including acidic fibroblast growth factor (FGF), epidermal growth factor, and fibroblast growth factor. Furthermore, the decellularized scaffold retained 64% of VEGH compared to the native kidney. This growth factor is crucial for kidney bioengineering as it plays a role in angiogenesis and renal podocyte development \u003csub\u003e24\u003c/sub\u003e.\u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab1\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 1\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eProteomic analysis of decellularized and native pig (\u003cem\u003eSus scrofa\u003c/em\u003e) kidney\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"9\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c7\" colnum=\"7\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c8\" colnum=\"8\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c9\" colnum=\"9\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e \u003cp\u003eAccession number\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eDescription\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003e# Peptides (Native)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003eCoverage [%] (Native)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003eAbundance (Native)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c6\"\u003e \u003cp\u003e# Peptides (Decellularized)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c7\"\u003e \u003cp\u003eCoverage [%] (Decellularized)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c8\"\u003e \u003cp\u003eAbundance (Decellularized)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c9\"\u003e \u003cp\u003eFold\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1RU24\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eAcidic fibroblast growth factor\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e58\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.33E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e41\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.11E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1.33\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287ANW0\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eActin alpha 2, smooth muscle\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e19\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e68\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.10E\u0026thinsp;+\u0026thinsp;10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e16\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e63\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e6.60E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.31\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1STD5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eActin related protein 1B\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e19\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.63E\u0026thinsp;+\u0026thinsp;05\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e20\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e5.35E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e20.32\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A480NK33\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eActin related protein 2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e36\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.17E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e33\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e2.70E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1.25\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A286ZIH7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eActin related protein 3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e17\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e50\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.76E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e14\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e37\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e2.10E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.77\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eI3LHK5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eActin-related protein 10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.89E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.27E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1.13\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eB5APU6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eActin-related protein 2/3 complex subunit\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e27\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e7.04E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e44\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e2.60E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e3.66\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eQ6QAQ1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eActin, cytoplasmic 1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e18\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e63\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.12E\u0026thinsp;+\u0026thinsp;10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e16\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e67\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e5.60E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.5\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eI3LLY3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eActinin alpha 1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e41\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e56\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e8.32E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e35\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e51\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.50E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.18\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1RI39\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eActinin alpha 4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e56\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e71\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e5.15E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e46\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e61\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.50E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.29\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A286ZR45\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eAdducin 1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e18\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e35\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.38E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e14\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e4.50E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.32\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287AQ41\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eAdducin 3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e17\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e38\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.07E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e13\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e4.80E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.45\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eI3LI80\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eAdvillin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.12E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e25\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e5.55E\u0026thinsp;+\u0026thinsp;05\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.26\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SSF7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eAE binding protein 1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e13\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e14\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e6.59E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e13\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.10E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.47\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A286ZS39\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eAgrin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e37\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e31\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.90E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e32\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e23\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.20E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1.7\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A5G2QI26\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eAlanyl aminopeptidase, membrane\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e35\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e47\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e3.66E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e22\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e36\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e2.20E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.06\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF2Z5G5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eAlpha-centractin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e47\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e9.17E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e40\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e5.60E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.61\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eQ7M2W6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eAlpha-crystallin B chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e58\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.08E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e21\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e7.93E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.07\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eP79307\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eAmyloid-beta precursor protein\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e9.43E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e23\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e8.41E\u0026thinsp;+\u0026thinsp;05\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.09\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SJB5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eAnnexin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e16\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e56\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e4.26E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e16\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e4.10E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eP19620\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eAnnexin A2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e22\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e64\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.19E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e17\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e57\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.10E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.09\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SUE4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eAsporin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e9.91E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e25\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.40E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e3.42\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A5G2QGZ8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eBasigin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e33\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e3.66E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.60E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.04\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A5K1UKC5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eBiglycan\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e44\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.66E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e37\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.60E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.94\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SAM3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCadherin 2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e17\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e3.17E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e15\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e6.01E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.19\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A5G2QAL2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCalponin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e37\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e8.41E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.60E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.19\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A5G2R7Z4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCatenin alpha 1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e39\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e59\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e7.15E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e16\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e5.10E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.07\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF6QAP1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCatenin beta 1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e23\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e40\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e3.20E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e13\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e20\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.10E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SGT4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCD44 antigen\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.49E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.83E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.07\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A286ZRZ8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCingulin like 1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e19\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e16\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.65E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e7.58E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.05\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1RP52\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eClaudin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.89E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.64E\u0026thinsp;+\u0026thinsp;05\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.19\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287A1S6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen alpha-1(I) chain preproprotein\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e49\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e52\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.57E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e56\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e61\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e4.10E\u0026thinsp;+\u0026thinsp;10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e26.34\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A480I6I0\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen alpha-1(XIV) chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e63\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e51\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.16E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e69\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e55\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e5.80E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e2.68\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eQ59IP2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen alpha-2(V) chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.11E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e26\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e29\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.40E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e159.78\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SFA7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type I alpha 2 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e46\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e49\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e9.66E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e49\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e54\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e7.40E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e7.68\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287A2Q7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type II alpha 1 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e5.48E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e7.90E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1.44\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A286ZQ85\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type III alpha 1 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e50\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e47\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.37E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e54\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e55\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.20E\u0026thinsp;+\u0026thinsp;10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e8.71\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A8W4FCC2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type IV alpha 1 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.20E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e17\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e18\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.10E\u0026thinsp;+\u0026thinsp;10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e94.13\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1RLL9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type IV alpha 2 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e21\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e20\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.82E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e35\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e37\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e9.70E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e53.18\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SNP0\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type IV alpha 3 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.53E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.30E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e86.34\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287AZF2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type IV alpha 4 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e14\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e13\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.06E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e14\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e13\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.10E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287A007\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type IV alpha 5 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.02E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e2.00E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A8W4FGE1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type IV alpha 6 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e6.76E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e15\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e13\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e2.60E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e38.88\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1S021\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type V alpha 1 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e3.78E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e18\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e15\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e4.60E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e12.07\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eI3LS72\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type VI alpha 1 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e37\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e49\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.21E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e38\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e53\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e9.10E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e7.54\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eI3LQ84\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type VI alpha 2 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e26\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e35\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.25E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e31\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e41\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e5.50E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e4.39\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A286ZVG7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type VI alpha 3 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e93\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e41\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e3.21E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e91\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e44\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.10E\u0026thinsp;+\u0026thinsp;10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e3.5\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287BK35\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type VI alpha 5 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e47\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e24\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e3.02E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e61\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e31\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e9.80E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e3.23\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A5G2R0Y2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type VI alpha 6 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.15E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e20\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e7.30E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e6.32\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SKM1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type VII alpha 1 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e22\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.06E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e22\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.10E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SKX7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type VIII alpha 1 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e3.65E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.60E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A5G2RKA4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type XI alpha 1 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e3.66E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.66E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1RQI0\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type XII alpha 1 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e112\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e51\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.82E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e118\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e56\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e4.80E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1.69\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SSE8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type XV alpha 1 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e16\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e15\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.57E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e13\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.10E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.42\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A5G2QW73\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type XVI alpha 1 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.32E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e8.62E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e6.54\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A286ZIL9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type XVIII alpha 1 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e26\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e19\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e4.62E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e14\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.10E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.24\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1RZU6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type XXI alpha 1 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e5.16E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e5.16E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A8W4FDC1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type XXIV alpha 1 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e3.79E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.80E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A8W4FA02\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollagen type XXVI alpha 1 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e6.14E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e6.14E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A286ZI28\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCollectrin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e36\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e5.67E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e31\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e4.60E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.82\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eP02540\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eDesmin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e33\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e72\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.78E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e19\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e39\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.10E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.06\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eQ5GN48\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eDystrophin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.51E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e4.36E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.29\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287BJX5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eEpidermal growth factor receptor\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e43\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.48E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e25\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.39E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1.37\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287AP71\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eEGF containing fibulin extracellular matrix protein 1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e20\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e7.42E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e30\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e9.30E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e12.51\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A5G2QCU4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eElastin microfibril\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e21\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e25\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.56E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e20\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e21\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.30E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.86\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287AXU0\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eElastin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.96E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e37\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e8.25E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e2.79\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eI3LC64\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eExtracellular matrix protein 1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e7.29E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e6.79E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.93\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0PFK5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eF-actin-capping protein subunit alpha-1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e69\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e8.74E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e20\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.41E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.04\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eQ29221\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eF-actin-capping protein subunit alpha-2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e51\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.12E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e20\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e7.22E\u0026thinsp;+\u0026thinsp;05\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.03\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0PFK7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eF-actin-capping protein subunit beta\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e36\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.30E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e14\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.50E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.12\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1RQ01\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eFERM domain containing kindlin 3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.21E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e7.17E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e3.24\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SN67\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eFibrillin 1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e78\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e40\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.03E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e99\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e49\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e7.00E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e6.85\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A5G2QM06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eFibrillin 2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e6.52E\u0026thinsp;+\u0026thinsp;05\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e45\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e22\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.80E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e283.62\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1RX36\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eFibrinogen alpha chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e21\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e26\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e5.71E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e18\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e22\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e5.00E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.88\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eI3L651\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eFibrinogen beta chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e24\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e61\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e8.66E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e20\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e56\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.00E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1.21\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A5G2QUU1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eFibrinogen gamma chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e23\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e67\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e7.29E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e24\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e68\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.70E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e2.38\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287BGK8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eFibroblast growth factor\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e4.85E\u0026thinsp;+\u0026thinsp;05\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e73\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.45E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e2.98\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1S6B5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eFibromodulin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.52E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e27\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e6.70E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e4.41\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SS24\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eFibronectin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e66\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e44\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.01E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e61\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e37\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.00E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1.01\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SPG5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eFibulin 2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e9.53E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e2.50E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e2.62\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287AL89\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eFibulin-1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e16\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.09E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e22\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e8.70E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e4.17\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A480ZQ94\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eFibulin-5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e24\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.22E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e16\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.20E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.96\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287AW81\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eFilamin A\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e108\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e58\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e4.17E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e29\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e2.70E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.06\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287BLE0\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eFilamin B\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e99\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e52\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e8.70E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e13\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.70E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.02\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SMN5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eFilamin C\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e37\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e18\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e5.12E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.81E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.04\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A5G2Q797\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGalectin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e33\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.19E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e34\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.40E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.66\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287A6P1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGelsolin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e23\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e41\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e5.42E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e9.53E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.02\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1RX94\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGrowth factor receptor bound protein 7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.51E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e32\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e6.54E\u0026thinsp;+\u0026thinsp;05\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.26\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SEQ3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eGrowth hormone-inducible transmembrane protein\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e15\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.15E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.20E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.28\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A286ZHV7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eHeparan sulfate proteoglycan 2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e105\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e39\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.35E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e106\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e39\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.80E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e2.83\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A481B9A6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eHistidine-rich glycoprotein\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e21\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e44\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e9.45E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e16\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e2.50E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.03\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eK7GQ83\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eIntegrin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e25\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e37\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e8.69E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e26\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e2.24E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.26\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SB67\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eInsulin like growth factor 2 receptor\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e19\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e4.14E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e75\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.50E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.37\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1RVH7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eInsulin like growth factor binding protein 7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e51\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e5.44E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e41\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e9.40E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1.73\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287AG36\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eLaminin subunit alpha 1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e15\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e8.11E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e18\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.90E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.48\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287A027\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eLaminin subunit alpha 2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e6.86E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e7.43E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1.08\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1RZM4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eLaminin subunit alpha 4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e18\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e14\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e5.03E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e9.39E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.19\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A8W4FFH5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eLaminin subunit alpha 5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e52\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e21\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.92E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e95\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e37\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.50E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e7.62\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SAE9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eLaminin subunit beta 1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e36\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e28\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.31E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e52\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e42\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.10E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e4.68\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287AWV5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eLaminin subunit beta-2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.03E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e33\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e24\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.60E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e15.43\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1S663\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eLaminin subunit gamma 1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e43\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e42\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.84E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e47\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e41\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.00E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e3.55\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A286ZWQ1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eLatent transforming growth factor beta binding protein 1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e3.27E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e2.70E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e8.21\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A5G2R0I5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eLatent transforming growth factor beta binding protein 4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.93E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.66E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.09\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1S3D5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eLectin, mannose binding 2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e24\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e5.68E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.17E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.02\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eB0KYV5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eLIM domain and actin-binding protein 1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e17\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e33\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e6.07E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e7.14E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.12\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SQ09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eLumican\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e44\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.00E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e42\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.70E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1.87\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eI3LQ79\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eMajor vault protein\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e34\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e48\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e4.40E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e25\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e34\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e2.90E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.65\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eI3LLG6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eMatrix remodeling-associated protein 8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.03E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e13\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e5.97E\u0026thinsp;+\u0026thinsp;05\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.29\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1RS52\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eMetabolism of cobalamin associated A\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e15\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.66E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e13\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.00E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.63\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SUS0\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eMicrofibril associated protein 2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e16\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e5.39E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e25\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e7.40E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e138.13\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287AB52\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eMicrofibril associated protein 4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.78E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e20\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e6.20E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e2.23\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A5G2R2Y0\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eMicrofibril associated protein 5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e14\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.62E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e4.95E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.19\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287AK19\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eMoesin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e32\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e51\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e3.10E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e16\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e28\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e5.00E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.16\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eM3UZ63\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eMyeloid derived growth factor\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e32\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e3.72E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e23\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e2.90E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.78\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A8W4FCU2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eMyosin heavy chain 9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e115\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e57\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e3.53E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e62\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e37\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e2.10E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.6\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287BFH0\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eMyosin IB\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.82E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.50E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.53\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A480I6L4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eMyosin IC\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e34\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e41\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.74E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e27\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e32\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.20E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.44\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287ARN5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eMyosin ID\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.77E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e13\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e15\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.10E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1.75\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SA47\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eMyosin IF\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e5.91E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.00E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.02\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SM78\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eMyosin light chain 12B\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e63\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.16E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e62\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.10E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.09\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SSA6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eMyosin motor domain-containing protein\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e53\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e56\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e4.94E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e28\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e31\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e6.10E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.12\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eP29269\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eMyosin regulatory light polypeptide 9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e63\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.30E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e62\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.10E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.05\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A5G2R6M1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eMyosin VB\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.68E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e15\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.00E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1.77\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287ARB4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eMyosin VC\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e4.05E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e6.10E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1.51\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SUK5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eMyosin VIIA\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e4.31E\u0026thinsp;+\u0026thinsp;05\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e21\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e4.40E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e102.83\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287AMU3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eMyosin-10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e49\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e5.92E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e39\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e8.40E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.14\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SF23\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eNephrocan\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.86E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.60E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e5.57\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287AU52\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eNephronectin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.49E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e22\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.50E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e58.74\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1RGY5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eNidogen 1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e22\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e29\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.38E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e20\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e22\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e2.00E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1.45\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A8W4FJT4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eNidogen 2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e26\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e30\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.99E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e21\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e23\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e2.30E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1.16\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1S6A2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eNPHS2 stomatin family member, podocin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e14\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.19E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e13\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.30E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1.51\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A8W4FNH8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eOpioid growth factor receptor (OGFr)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e9.64E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e61\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e2.22E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.23\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A0H5ANC0\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eOsteoglycin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e31\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.26E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e20\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e2.10E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.17\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1S3J7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003ePapilin, proteoglycan like sulfated glycoprotein\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e16\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e27\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e4.95E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e5.86E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.12\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A5G2QJH9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eParvin alpha\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e19\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e6.74E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e16\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.70E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.25\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A5G2RG25\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003ePaxillin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.17E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e6.39E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.29\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1RS37\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003ePeriostin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e6.96E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e6.05E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.87\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1RL75\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003ePlatelet-derived growth factor receptor beta\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.71E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.12E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.07\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287A8C7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003ePlectin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e115\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e28\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e5.81E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e25\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e5.50E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.09\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SNF3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003ePodocalyxin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e20\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e7.72E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e4.77E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.06\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eI3LEB7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003ePodocan\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e4.23E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.80E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.04\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eI3LEE6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eProcollagen C-endopeptidase enhancer\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.68E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.68E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A8W4FAZ5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eRadixin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e37\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e54\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.78E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e18\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e34\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.00E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.59\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1S4R1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eRegulator of microtubule dynamics 2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e19\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e4.76E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e6.29E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1.32\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287BBC9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eRegulator of microtubule dynamics 3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e13\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.32E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e2.33E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.18\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A286ZRU9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eSerpin H1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e18\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e50\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e8.77E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e13\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e35\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.50E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.17\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eP20112\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eSPARC\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e20\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.40E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e2.50E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1.77\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1S981\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eSpondin-1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e8.33E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e4.30E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.52\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A8W4FGV4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eSynemin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e26\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.81E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.17E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.18\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A5G2Q9C7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eT-complex protein 1 subunit gamma\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e26\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e63\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e4.05E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e23\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e52\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e2.40E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.59\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287BAK1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eTalin 1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e100\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e57\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.11E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e16\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.60E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.03\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287B272\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eTalin 2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e42\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e21\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e6.14E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.16E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.02\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eI3LUI4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eTenascin C\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e30\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e20\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e6.29E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e13\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e9.28E\u0026thinsp;+\u0026thinsp;05\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.01\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eK7GRK7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eTenascin XB\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e24\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.56E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e31\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e15\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e9.20E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e3.6\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eQ2QLE3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eTestin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e15\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.46E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.97E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.13\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SS26\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eThrombospondin 1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e21\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e27\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e7.63E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e7.35E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SIR7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eThrombospondin type 1 domain containing 4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e5.14E\u0026thinsp;+\u0026thinsp;05\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.20E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e23.75\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1RHA7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eTransforming growth factor beta induced\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e18\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e41\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.46E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e13\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e28\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e9.03E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.62\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287B6W1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eTransforming growth factor beta-1-induced transcript 1 protein\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e4.60E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e7.73E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e1.68\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eO11780\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eTransforming growth factor-beta-induced protein ig-h3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e18\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e41\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.55E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e13\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e27\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.30E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e2.15\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287AZK2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eTransgelin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e14\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e51\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e9.78E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e36\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.10E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.11\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eP67937\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eTropomyosin alpha-4 chain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e17\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e47\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e5.47E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e28\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.30E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.06\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eI3LGB4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eTubulointerstitial nephritis antigen\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e13\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e37\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e2.20E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e19\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e53\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.30E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e5.91\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1RPA9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eUromodulin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e19\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e34\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e3.92E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e2.80E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.07\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eA0A287BFL9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eVascular endothelial growth factor\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e59\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.05E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e24\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e38\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e6.79E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.64\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1SX62\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eVersican\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e26\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e3.19E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e7.47E\u0026thinsp;+\u0026thinsp;06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.23\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eQ29261\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eVillin-1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e31\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e49\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e9.23E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e20\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e29\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e3.70E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.4\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eP02543\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eVimentin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e39\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e79\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e5.09E\u0026thinsp;+\u0026thinsp;09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e21\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e40\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e6.40E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.13\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eP48819\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eVitronectin\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e31\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.73E\u0026thinsp;+\u0026thinsp;08\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e19\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e4.00E\u0026thinsp;+\u0026thinsp;07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.23\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003cp\u003e \u003cb\u003eCharacterization of the primary porcine renal cells\u003c/b\u003e \u003c/p\u003e \u003cp\u003eDuring the primary culture of WKPC with kidney mix media (IP under submission), we observed various morphologies, primarily during early passages. However, with successive passages, the heterogeneity of the cell population decreased, and fibroblast-shaped cells began to dominate the culture, reaching confluency earlier with each passage.\u003c/p\u003e \u003cp\u003eAnalysis of WKPC revealed changes in marker expression over successive passages. Both qPCR and immunofluorescence confirmed the presence of epithelial cells expressing CDH1 (E-cadherin), AQP1 indicative of cells found in proximal tubules, and AQP4 indicating the presence of cells conforming to the collecting ducts \u003csub\u003e25\u003c/sub\u003e. Additionally, cells expressing Vimentin, typically found in the renal capsule, glomeruli, and vasculature, were identified, suggesting the presence of renal tubular cells and fibroblasts \u003csub\u003e26,27\u003c/sub\u003e. The analysis also revealed the presence of endothelial cells expressing CD31 and podocytes expressing podocin, crucial for glomeruli formation, the site of the initial filtration step \u003csub\u003e25,28,29\u003c/sub\u003e. Furthermore, a lower expression of immune cells, particularly monocytes expressing CD14, was observed \u003csub\u003e30\u003c/sub\u003e (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003ea).\u003c/p\u003e \u003cp\u003eRT-qPCR analysis demonstrated an upregulation of CDH1 and Vimentin as passages increased, compared to uncultured WKPC, indicating a higher presence of epithelial and fibroblast cells in the culture (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003eb). In contrast, the expression of AQP1, AQP4, CD14, CD31, and Podocin decreased compared to the original uncultured WKPC. The most significant decrease was observed in immune cells expressing CD14. Notably, podocin exhibited a lower decrease in expression compared to the other markers (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003ea). These findings suggest that WKPC should be utilized at earlier passages, preferably up to passage two, to ensure effective repopulation with a variety of cells as found in the native kidney.\u003c/p\u003e \u003cp\u003eThese results were confirmed with immunofluorescence analysis. The majority of the cells showed positive staining for AQP1, AQP4, Vimentin, and CDH1 (E-cadherin), indicating their epithelial cell nature. A small percentage of the population demonstrated positive staining for CD31 and podocin, suggesting the presence of endothelial cells and podocytes, respectively (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003ec-g).\u003c/p\u003e \u003cp\u003eThese findings validate the efficacy of the method used to isolate WKPC, which involved the dissociation of kidney sections rather than utilizing the whole organ. By using this approach, we generated populations of cells expressing a diverse range of kidney-specific markers, crucial for performing various kidney functions. This highlights the potential of WKPC as a valuable resource for further studies to optimize the recellularization techniques of a whole pig kidney and later test for functionality both \u003cem\u003ein vivo\u003c/em\u003e and \u003cem\u003ein vitro.\u003c/em\u003e\u003c/p\u003e \u003cp\u003eFlow cytometric analysis corroborated what was found in the RT-qPCR assay, showing that the WKPC population changes over passages. Table\u0026nbsp;\u003cspan refid=\"Tab2\" class=\"InternalRef\"\u003e2\u003c/span\u003e provides marker expression percentages for different renal cell antibodies in WKPC at various passages. Passage zero represents cells without culture, freshly isolated from a native kidney. The cell viability of WKPC remained constant through passages, suggesting that fluctuations in percentages over passages might be due to the faster duplication rates of stromal and fibroblast cells expressing vimentin rather than cell death \u003csub\u003e31, 32\u003c/sub\u003e.\u003c/p\u003e \u003cp\u003eIn cells obtained directly from the tissue (P0), a predominance of epithelial cells expressing AQP4 and AQP1 was observed, along with a significant percentage of endothelial cells. However, by passage 3, there was a notable shift in cell composition. Most cells expressed Vimentin, indicative of a stroma or fibroblast-like phenotype, while the percentages of AQP1 and CD31-positive cells decreased.\u003c/p\u003e \u003cp\u003eResults showed that Vimentin expression gradually increased from 15.20% in passage 0 to 66.10% in passage 3, indicating an enrichment of Vimentin-positive cells over successive passages, in agreement with RT-qPCR results. This change may be attributed to culture conditions favoring the growth of Vimentin-expressing cells. In contrast, Podocin expression increased from 13.70\u0026ndash;47.90% in passage 2 and then slightly decreased to 37.60% in passage 3.\u003c/p\u003e \u003cp\u003eMoreover, the expression of CD14, a marker associated with immune cells, remained relatively low throughout the passages, ranging from 0.12\u0026ndash;2.08%. The expression of AQP1 decreased from 65.30% in uncultured cells to 22.20% in passage 3, indicating a potential loss or reduced presence of proximal tubular cells. CD31, a marker for endothelial cells, decreased from 65.90\u0026ndash;22.40% in passage 3. AQP4, a marker for collecting duct cells, showed a slight decrease from 69% in passage 0 to 50.80% in passage 3.\u003c/p\u003e \u003cp\u003eThese findings highlight the heterogeneity and phenotypic changes within the WKPC population during culture, underscoring the importance of considering passage number when utilizing these cells for recellularization or other applications.\u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab2\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 2\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eResults of the flow cytometry analysis of whole kidney pig cells (primary renal porcine cells)\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"6\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e \u003cp\u003eFluorophore\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eMarker\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eP0\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003eP1\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003eP2\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c6\"\u003e \u003cp\u003eP3\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eFITC\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e\u003cem\u003eVimentin\u003c/em\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e15.2%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e44.1%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e56%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e66.1%\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003ePE\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e\u003cem\u003ePodocin\u003c/em\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e13.7%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e40.0%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e47.9%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e37.6%\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003ePer-CP\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e\u003cem\u003eCD14\u003c/em\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e2.08%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.18%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e0.12%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e0.33%\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003ePE-Cy7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e\u003cem\u003eAQP1\u003c/em\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e65.3%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e25.5%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e37.1%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e22.2%\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eAPC-Cy7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e\u003cem\u003eCD31\u003c/em\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e65.9%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e29%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e33.4%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e22.4%\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eAPC-Cy7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e\u003cem\u003eAQP4\u003c/em\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e69%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e36%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e52%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e50.8%\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eZombie UV\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e\u003cb\u003eViability\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e\u003cb\u003e98.5%\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e\u003cb\u003e93.0%\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e\u003cb\u003e97.7%\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e\u003cb\u003e97.5\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003cp\u003e \u003cb\u003eEvaluation of a whole Recellularized pig kidney\u003c/b\u003e \u003c/p\u003e \u003cp\u003eThe accompanying image showcases the macroscopic transformation of the kidney over the course of several days (Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003eb). Starting from a decellularized organ, the kidney progressively evolved to contain 500\u0026nbsp;million well-characterized WKPC and was initially perfused solely with media. Ultimately, the kidney contained an impressive population of 318\u0026nbsp;billion red blood cells (RBCs). It is worth noting that the proportion of WKPC within the total cell population accounted for only 0.15% of the perfused RBCs, leading to erythrocyte accumulation all over the matrix. However, prior to the perfusion of RBCs, on the 5th day, the kidney closely resembled a native kidney, without blood flow.\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003eIn the panel of Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003ea it is shown six sections representing the histological examination of a native kidney, a decellularized kidney, and a recellularized kidney. Starting from the left, the native kidney section serves as a reference for comparison. It displays the characteristic histological features of a healthy kidney, including well-defined renal structures such as glomeruli, tubules, and blood vessels. The decellularized kidney displays a significant absence of cellular material while keeping the ECM intact. On the right side, we have the recellularized kidney section, resembling every time more a native kidney. This section demonstrates a high percentage of repopulation of the decellularized ECM with new cells. The image reveals a reestablishment of cellular density and organization. Regions with clusters of cells, forming cell colonies, can be observed. Furthermore, the presence of various renal structures, including tubules, and circular formations resembling glomeruli, suggests the recellularization of diverse renal cells forming different renal structures.\u003c/p\u003e \u003cp\u003eThe histological evaluation revealed a robust presence of cells distributed throughout the matrix, indicating a high recellularization percentage and the formation of colonies by WKPCs. H\u0026amp;E staining was performed on various sections, including the superior pole, superior half, inferior half, and inferior pole, encompassing the parenchyma, collecting ducts, and medulla. The sampling of both cortex and medulla from different sections validated the successful reach of cells to the organ's periphery. Cells exhibited alignment within tubular structures, closely resembling a native kidney, in the superior half. Furthermore, in the inferior half, there is evidence of cells recovering the wall of blood vessels. The presence of diverse cell types indicated the establishment of different cell populations crucial for functional kidney regeneration. Notably, interconnected cell networks were observed within the scaffold, indicating cell-to-cell communication and potential migration within the matrix. The image displayed a well-organized arrangement of cells, with varying cell densities across different regions. There were no signs of cell aggregation. It was observed circular structures suggesting the repopulation of glomeruli in the cortex, especially in the superior and the inferior pole (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003e). The higher number of RBC observed could potentially obstruct the microarchitecture of the organ, posing a challenge for cell migration. Nevertheless, the protocol demonstrated effective cell perfusion in the cortex and medulla.\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003e \u003cb\u003eIdentification of primary porcine renal cells in Recellularized pig kidney\u003c/b\u003e \u003c/p\u003e \u003cp\u003eFluorescence microscopy analysis revealed that the glomeruli-like structures in the seeded kidney were densely populated with podocytes in the cortex across all sampled sections, highlighting their successful integration. Endothelial cells expressing CD31 were predominately aligned within vascular structures in both the medulla and the cortex, with enhanced expression in the half sections of the kidney. Furthermore, positive staining for AQP4 indicated site-specific adhesion of the extracellular matrix by epithelial cells in the tubular regions of the medulla, particularly in the collecting ducts. Vimentin expression was detected in the renal tubules, showing a high abundance in all sections. Additionally, the presence of aquaporin 1-expressing epithelial cells formed structures resembling the native proximal tubular epithelium observed in the cortex, primarily concentrated in the poles of the organ. These findings suggest that the seeded cells exhibit site-specific localization and the ability to form structures resembling the native renal components (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003ea-e). Ki-67 staining demonstrated the presence of 58.1\u0026thinsp;\u0026plusmn;\u0026thinsp;17.2% brown-labelled proliferating cells distributed throughout the recellularized kidney, indicating active cell division of the WKPC inside the organ. This suggests that the recellularization process has successfully supported cell proliferation within the tissue scaffold (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003eb). In contrast, the Caspase-3 staining revealed 14.22\u0026thinsp;\u0026plusmn;\u0026thinsp;4.46% of brown-labelled apoptotic cells in the recellularized pig kidney, indicating minimal cell death within the tissue. This indicates a favourable environment for cell survival and suggests that the recellularization protocol provides enough oxygen and nutrients to support the milieu for the maintenance and viability of the introduced WKPC (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003ef).\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003eThe number of cells present in the culture media accounted for less than 1% of the initially seeded cells, indicating the recellularization process was successful since the ECM retained 99% of the cells. Although LDH values gradually increased during the reseeding process, there was not a significant production of LDH, consistently remaining below 16 U/L. The normal level of LDH in human blood is bellow 280 U/L, demonstrating that 16U/L does not express signs of cytotoxicity. The histological analysis and the assessment of LDH levels mutually reinforce each other, providing robust evidence for the successful retention and viability of the seeded cells within the recellularized kidney. The protocol of recellularization resulted in low tissue damage, low cell death and cell migration to the periphery of the organ within the recellularized kidney (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003eg).\u003c/p\u003e"},{"header":"Discussion","content":"\u003cp\u003eThe successful reseeding of entire pig kidneys represents a significant advancement in the fields of tissue engineering and transplantation. Given the intricate structure of the kidney, which includes a complex network of capillaries and thousands of nephrons with intricate functional structures, the decellularization process is the most suitable method for bioengineering a functional kidney. To further advance kidney bioengineering, it is imperative to establish an optimal protocol for recellularizing kidneys of clinically relevant sizes\u003c/p\u003e \u003cp\u003eThe success of the decellularization process was confirmed by the DNA content, which measured below 50 ng of DNA per milligram of tissue, meeting the criteria for organ decellularization. Preservation of the microarchitecture, including the integrity of the glomerular loops, as well as the retention of proteins within the extracellular matrix (ECM), further validated the efficacy of the decellularization process \u003csub\u003e7\u003c/sub\u003e. Zambon et al. \u003csub\u003e33\u003c/sub\u003e demonstrated that a combination of SDS and Triton X-100 is the optimal decellularization methodology, achieving comparable macroscopic and microscopic results while significantly reducing perfusion time from 72 to 45 hours. This modification minimizes ECM damage while ensuring complete cell removal.\u003c/p\u003e \u003cp\u003eIt's worth noting that Sullivan et al. decellularized pig kidneys for 36 hours using only 0.5% SDS, which might result in structural protein and growth factor damage. Zambon et al. \u003csub\u003e13\u003c/sub\u003e further evaluated this method and observed ECM damage when perfusing with 0.5% SDS, as confirmed by scanning electron microscopy (SEM). In contrast, Tan \u003csub\u003e34\u003c/sub\u003e found that decellularizing a rat kidney with 0.1% SDS and 1X Triton did not damage the ECM, as evidenced by SEM analysis. Additionally, canine and pig kidneys were decellularized in 8 and 12 hours, respectively, using SDS 0.5% perfusion and cryoablation. However, this process did cause damage to the overall ECM structure \u003csub\u003e35,36\u003c/sub\u003e.\u003c/p\u003e \u003cp\u003eHistological analyses have consistently shown the preservation of vital ECM components such as collagen, laminin, fibronectin, elastin, and glycosaminoglycans (GAGs) after decellularization using 0.1% SDS and Triton X-100 \u003csub\u003e7, 23, 37, 38\u003c/sub\u003e. Nevertheless, there has been a lack of a proteomic profile of the ECM post-decellularization of pig kidneys. Leuning et al. \u003csub\u003e39\u003c/sub\u003e reported a proteomic analysis for rat and human kidneys, obtaining results similar to our findings in porcine decellularized kidneys. Notably, our study revealed a higher presence of growth factors, particularly VEGF and FGF, which are crucial for cell adherence \u003csub\u003e24\u003c/sub\u003e. This may be attributed to the decellularization protocol using Triton/SDS, as Caralt et al. demonstrated good retention of FGF and VEGF in the matrix after decellularizing a rat kidney \u003csub\u003e37\u003c/sub\u003e. In the proteomic analysis of decellularized pig kidneys, we observed an increase in the relative concentration of elastin and certain collagens, including collagen IV, collagen V, and collagen I, which are primary constituents of the ECM. This finding contradicts the results reported by Hussein et al. \u003csub\u003e40\u003c/sub\u003e, who observed a significant reduction in elastin but aligns with the statement that collagen is preserved in a decellularized pig kidney scaffold. Additionally, the level of VEGF retained in the decellularized kidney was 64%, compared to the 38% reported by Hussein et al. \u003csub\u003e40\u003c/sub\u003e. Similarly, these researchers reported the retention of 44% for FGF, whereas, in our study, there was a 2.98-fold increase in the relative concentration of this growth factor \u003csub\u003e41\u003c/sub\u003e. These findings support the preservation of critical ECM components, which play a pivotal role in the recellularization process by promoting cell migration and enhancing cell viability. Preserving the growth factors offers the opportunity to guide the phenotypic specification of cells toward their original location-specific types \u003csub\u003e41\u003c/sub\u003e.\u003c/p\u003e \u003cp\u003eIt was found in this study that combining perfusion and diffusion techniques enabled better preservation of cell viability. Antegrade perfusion supplies cells to the vasculature and at the glomerular level, while retrograde perfusion repopulates the collecting system and the tubular lumen \u003csub\u003e23\u003c/sub\u003e. Negative pressure has been employed to enhance cell migration and extravasation. Previous studies have reported the benefits of using negative pressure during cell seeding, when regulated with controlled time and pressure, it does not damage the extracellular matrix (ECM) and promotes a more uniform cell distribution \u003csub\u003e17,39,42,43\u003c/sub\u003e. Poornejad et al. \u003csub\u003e43\u003c/sub\u003e specifically demonstrated a significant improvement in cell migration from the tubules to the cortex using negative pressure (-40mmHg) during ureteral perfusion, which aligns with the results obtained by our group. We can conclude that \u0026minus;\u0026thinsp;17inHg yielded more favorable results in terms of recellularization efficiency and distribution, indicating its potential as a key factor in optimizing the recellularization process. It is worth noting that our protocol differed from other studies by focusing on whole organ recellularization, perfusing various cell types through the vein, artery, and ureter, resulting in better outcomes with a higher recellularization percentage. This approach successfully demonstrated the presence of endothelial cells in the blood vessels, epithelial cells in the tubules, and podocytes in glomerular-like structures.\u003c/p\u003e \u003cp\u003eWe successfully isolated, cultured, and expanded primary porcine renal cells, which exhibited the expression of renal cell markers. These findings are consistent with those of Abolbashari et al. \u003csup\u003e44\u003c/sup\u003e, who also demonstrated the presence of AQP1, AQP4, and Podocin in cultured primary porcine renal cells. However, the percentages obtained during flow cytometry analysis differed between the two studies. The WKPC cells showed lower expression of AQP1 (65.3% vs. 72%) but higher expression of AQP4 and Podocin (69% vs. 20% and 13.7% vs. 2%, respectively). This indicates a heterogeneous population of vital kidney cells expressing markers crucial for the bioengineering of a functional kidney. Moreover, the researchers demonstrated that primary porcine renal cells maintain renal cell functions, such as sodium uptake, hydrolase activity, and erythropoietin production. Consequently, assessing the in vitro functionality of the whole pig kidney recellularization should be a focus of future trials. It is worth mentioning that for successful recellularization, early passages of the cells should be utilized to preserve the majority of the cells. Flow cytometry and RT-qPCR analysis revealed that, over passages, the number of cells expressing Vimentin surpassed the desired population. Furthermore, optimizing the culture media is necessary to maximize the preservation of endothelial cells and monocytes, as these cell types showed a higher decrease in abundance over passages.\u003c/p\u003e \u003cp\u003eAbolbashari et al. \u003csup\u003e44\u003c/sup\u003e developed a methodology for the formation of renal tubular structures; however, their seeding method involved cell injection, which may result in ECM damage and inefficient repopulation of the whole organ. In contrast, the use of perfusion allowed for a uniform distribution and migration of cells throughout the entire recellularized kidney. We observed a significantly higher number of cells within the recellularized kidney at 12 days of culture, indicating a higher recellularization percentage and increased formation of renal structures, including tubular-like and glomerular-like formations. Furthermore, the presence of cells expressing AQP1 and AQP4 in the appropriate structures further supports the hypothesis that ECM components and distribution facilitate the migration of cells to their respective locations.\u003c/p\u003e \u003cp\u003eRegarding cell viability, the analysis conducted through ki-67 and caspase-3 staining supported the effectiveness of the recellularization approach in promoting cell proliferation and reducing cell death, crucial factors for the successful development of functional renal tissue. The 54.1% proliferation rate within the recellularized kidney significantly surpasses the findings of Leuning et al. \u003csup\u003e39\u003c/sup\u003e, who reported a proliferation rate of only 7.8%. Additionally, more stained cells were observed compared to the studies conducted by Hussein et al. \u003csup\u003e40\u003c/sup\u003e and Abolbashari et al. \u003csup\u003e44\u003c/sup\u003e. Conversely, similar results were obtained when compared to Remuzzi et al.'s \u003csup\u003e42\u003c/sup\u003e findings in rat kidney recellularization. Bonandrini et al. \u003csup\u003e18\u003c/sup\u003e reported higher proliferation using PCNA in porcine kidney recellularization after 72 hours in culture.\u003c/p\u003e \u003cp\u003eConcerning cell death during pig kidney reseeding, the percentage of cell death on days 3, 7, 14, and 28 was lower in Abolbashari et al. \u003csup\u003e33\u003c/sup\u003e study compared to the findings in our recellularization process, where 16.72% expressed caspase-3. This coincides with the results reported by Bonandrini et al. \u003csup\u003e18\u003c/sup\u003e using embryonic stem cells. Although the percentage of cells expressing caspase-3 is not as low as in other researchers' findings, it still falls within an acceptable range. However, addressing this issue and further reducing cell death should be a focus of future investigations.\u003c/p\u003e \u003cp\u003eDuring the optimization process, it was observed that oxygenating the media through agitation was less effective than desired. Analysis of the culture media revealed poor retention and diffusive delivery of oxygen due to the low oxygen affinity of the culture media. Additionally, the organ lacked the constant gas exchange that occurs in 2D cultures, limiting effective oxygen transfer. To overcome these challenges, a natural oxygen carrier, hemoglobin found in red blood cells (RBCs), was utilized. The use of erythrocytes allowed for efficient oxygen delivery to the cells without relying on bubble generation and rupture, thus avoiding potential cell damage caused by shear forces. This approach also eliminated the limitations associated with gas liberation during agitation.\u003c/p\u003e \u003cp\u003eThe increased presence of RBCs resulting from this approach may lead to a higher repopulation of RBCs, which could potentially obstruct the microarchitecture of the organ and hinder cell migration. However, this test demonstrated that the method used is helpful to perfuse cells along different sections of the kidney since we can detect a high number of RBCs. Nonetheless, we continue to observe the formation of circular structures along the ECM. To further optimize the protocol, it is necessary to increase the amount of perfusing WKPC by at least eightfold and explore using different oxygen carriers \u003csup\u003e44\u003c/sup\u003e.\u003c/p\u003e \u003cp\u003eIn the context of transplanting bio-engineered kidneys, it is understood that porcine primary renal cells may not be suitable for clinical applications. However, this study served as a proof-of-principle, aiming to optimize the recellularization technique. It was demonstrated that the use of WKPCs showed promising characteristics, particularly their rapid proliferation rate, making them potential candidates for recellularization when a large number of cells are required and for further evaluation of in vivo functionality of the bioengineered organs.\u003c/p\u003e \u003cp\u003eNotably, this study successfully obtained a significant number of cells from a small amount of tissue. From just 5.5 mg of tissue, approximately 16010^6 cells were obtained. Extrapolating these findings to the whole tissue, it can be estimated that approximately 3.9810^9 (three billion nine hundred eighty-five million) cells could be obtained. These results align with previous findings reported in the literature \u003csup\u003e44\u003c/sup\u003e.\u003c/p\u003e"},{"header":"Conclusion","content":"\u003cp\u003eIn conclusion, the increasing incidence of End-Stage Renal Disease (ESRD) exacerbates the shortage of available organs for transplantation. The limitations of dialysis emphasize the urgent need for advancements in organ bioengineering. Bioengineered kidneys represent a promising solution to bridge the gap between organ supply and demand, ultimately enhancing the quality of life for patients in need.\u003c/p\u003e \u003cp\u003eEfficient decellularization of clinically relevant-sized kidneys while preserving the structural and compositional integrity of the extracellular matrix (ECM) is a critical milestone in the development of bioengineered organs. This study has demonstrated that the use of perfusion, negative pressure, nutrient immersion, and oxygenation significantly enhances the recellularization process, promotes cell viability, and facilitates the formation of functional renal structures.\u003c/p\u003e \u003cp\u003eFuture research endeavors should focus on optimizing the culture conditions and automating key processes to make these bioengineered kidneys suitable for clinical applications, thus laying the groundwork for their potential use in human transplantation. Ultimately, this ground-breaking research opens up new avenues for exploring and developing regenerative therapies, fundamentally transforming the landscape of organ transplantation and positively impacting the lives of countless individuals in need of life-saving treatments.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003e\u003cem\u003eAcknowledgments\u0026nbsp;\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThis study received no funding. We would like to express our gratitude to Dr. Dr Lange and Dr. David Harriman for his insights during the planning of the experiments.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eAuthor Contributions\u0026nbsp;\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eACLB, AIS and CICM conceived the study and designed the experiments. ACLB and CUMA carried out the organ harvesting, cell culture, immunofluorescence, and flow cytometry. ACLB was in charge of the decellularization and recellularization. ACLB and CUMA analyzed the data. ACLB, CCM and AIS wrote the manuscript. All the authors read and approved the submitted manuscript.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eCompeting interests\u0026nbsp;\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAll authors declare no financial or non-financial competing interests.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eData availability\u0026nbsp;\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe datasets used and/or analyzed during the current study available from the corresponding author on reasonable request.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\n\u003cli\u003eBenjamin, O. \u0026amp; Lappin, S. L. 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Have we hit a wall with whole kidney decellularization and recellularization: A review. \u003cem\u003eCurrent Opinion in Biomedical Engineering \u003c/em\u003e\u003cstrong\u003e20\u003c/strong\u003e, 100335 (2021).\u003c/li\u003e\n\u003cli\u003eRemuzzi, A. \u003cem\u003eet al. \u003c/em\u003eExperimental Evaluation of Kidney Regeneration by Organ Scaffold Recellularization. \u003cem\u003eSci Rep \u003c/em\u003e\u003cstrong\u003e7\u003c/strong\u003e, 43502 (2017).\u003c/li\u003e\n\u003cli\u003ePoornejad, N. \u003cem\u003eet al. \u003c/em\u003eRe-epithelialization of whole porcine kidneys with renal epithelial cells. \u003cem\u003eJ Tissue Eng \u003c/em\u003e\u003cstrong\u003e8\u003c/strong\u003e, 2041731417718809 (2017).\u003c/li\u003e\n\u003cli\u003eAbolbashari, M. \u003cem\u003eet al. \u003c/em\u003eRepopulation of porcine kidney scaffold using porcine primary renal cells. \u003cem\u003eActa Biomater \u003c/em\u003e\u003cstrong\u003e29\u003c/strong\u003e, 52\u0026ndash;61 (2016).\u003c/li\u003e\n\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"","lastPublishedDoi":"10.21203/rs.3.rs-3943939/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-3943939/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThis study explores a potential solution to the shortage of kidneys for transplantation in end-stage renal disease (ESRD). Currently, kidney transplantation is the best option, but there aren't enough organs available. The researchers used tissue engineering to evaluate the possibility for creating kidneys for transplantation. They used pig kidneys, removing cells through a process called decellularization while preserving the organ's microarchitecture. The researchers then perfused pig kidney cells and human red blood cells to the empty kidney structure to recreate it. The methodologies used, showed promising results, suggesting a hopeful method to address the organ shortage. This proof-of-concept lays the foundation for potentially applying this technology to human kidneys, which could significantly improve outcomes for ESRD patients by providing more transplantable organs.\u003c/p\u003e","manuscriptTitle":"Evaluating different methods for kidney recellularization","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-03-13 16:43:59","doi":"10.21203/rs.3.rs-3943939/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2024-04-05T02:35:29+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2024-04-03T07:41:24+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"8c46d8e8-f0cf-4fb8-8929-d6ffc86a9577","date":"2024-03-24T09:41:19+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2024-03-22T22:54:10+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"62438a47-9566-4a8c-9a42-64cf07ee8975","date":"2024-03-11T15:21:26+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2024-03-11T09:25:31+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2024-03-11T09:12:02+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2024-03-10T10:19:54+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2024-03-10T10:17:51+00:00","index":"","fulltext":""},{"type":"submitted","content":"Scientific Reports","date":"2024-02-09T19:09:05+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"41fd41b1-a032-4d13-a368-b09d97070c23","owner":[],"postedDate":"March 13th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[{"id":29346985,"name":"Health sciences/Medical research"},{"id":29346986,"name":"Health sciences/Urology"},{"id":29346987,"name":"Physical sciences/Engineering"}],"tags":[],"updatedAt":"2024-10-14T16:02:18+00:00","versionOfRecord":{"articleIdentity":"rs-3943939","link":"https://doi.org/10.1038/s41598-024-74543-w","journal":{"identity":"scientific-reports","isVorOnly":false,"title":"Scientific Reports"},"publishedOn":"2024-10-09 15:57:42","publishedOnDateReadable":"October 9th, 2024"},"versionCreatedAt":"2024-03-13 16:43:59","video":"","vorDoi":"10.1038/s41598-024-74543-w","vorDoiUrl":"https://doi.org/10.1038/s41598-024-74543-w","workflowStages":[]},"version":"v1","identity":"rs-3943939","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-3943939","identity":"rs-3943939","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}