Enhanced Enzyme-like Activity in Bimetallic Cu-Co Prussian Blue Analogues: Highly Sensitive Glucose Detection and Efficient Antibacterial Applications

Enhanced Enzyme-like Activity in Bimetallic Cu-Co Prussian Blue Analogues: Highly Sensitive Glucose Detection and Efficient Antibacterial Applications | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Enhanced Enzyme-like Activity in Bimetallic Cu-Co Prussian Blue Analogues: Highly Sensitive Glucose Detection and Efficient Antibacterial Applications Yaohua Gu, Xiaoqing Zhang, Gonghui Wang, Kehan Shi, Danian Tian, and 4 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9026837/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 11 You are reading this latest preprint version Abstract Recent years have witnessed growing focus on bimetallic Prussian blue analogs (PBAs) within the cross-disciplinary research areas of materials science and biomedicine. These materials possess a range of favorable properties, including an open framework structure, inherent catalytic activity, excellent biocompatibility, and tunable structural characteristics. Within the current research, a Cu–Co bimetallic Prussian blue analogue (Cu–Co PBA) with a characteristic framework structure was effectively fabricated via a straightforward low-temperature co-precipitation approach. Owing to its cost-effectiveness, high stability, straightforward preparation, and the synergistic interaction between Cu 2+ and Co 2+ ions, Cu–Co PBA shows significant potential as a multifunctional nanozyme and catalytic material. The catalyst demonstrates exceptionally high peroxidase-mimicking activity as a result of the synergistic interaction between the coexisting Cu + /Cu 2+ and Co 2+ /Co 3+ redox pairs within the system. This synergy facilitates electron transfer and remarkably boosts the catalytic decomposition efficiency of H₂O₂. Cu–Co PBA shows catalytic activity several times higher than single Cu-PBA or Co-PBA, thus improving glucose detection sensitivity and accelerating response kinetics, with a linear detection range of 10 µM–2000 µM and a low limit of detection of 2 µM. Moreover, Cu–Co PBA nanoparticles achieve efficient chemodynamic antibacterial performance by catalyzing H₂O₂ to produce hydroxyl radicals (·OH), which destroy bacterial cell structures and endow broad-spectrum antimicrobial activity. The inhibition rates for Staphylococcus aureus and Escherichia coli are 99.05% and 98.92%, respectively. This work integrates glucose detection and antibacterial treatment through the enzyme-like and Catalytic performance of bimetallic Prussian blue nanozymes, offering a promising multifunctional platform for biomedical applications. Nanozyme Catalysis Colorimetric sensor Glucose detection CDT antibacterial therapy Full Text Additional Declarations No competing interests reported. Supplementary Files SupplementaryMaterials.docx Supplementary Information The online version contains supplementary material available at https://doi.org/ Cite Share Download PDF Status: Under Review Version 1 posted Reviews received at journal 07 May, 2026 Reviewers agreed at journal 05 May, 2026 Reviewers agreed at journal 04 May, 2026 Reviewers agreed at journal 06 Apr, 2026 Reviewers agreed at journal 02 Apr, 2026 Reviews received at journal 01 Apr, 2026 Reviewers agreed at journal 25 Mar, 2026 Reviewers invited by journal 20 Mar, 2026 Editor assigned by journal 13 Mar, 2026 Submission checks completed at journal 12 Mar, 2026 First submitted to journal 04 Mar, 2026 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-9026837","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":609426056,"identity":"c18b7486-d578-4cca-a467-ce1cc916b157","order_by":0,"name":"Yaohua Gu","email":"","orcid":"","institution":"Ningxia Medical University","correspondingAuthor":false,"prefix":"","firstName":"Yaohua","middleName":"","lastName":"Gu","suffix":""},{"id":609426057,"identity":"92db769a-5c46-4960-8532-f6f53330fa3c","order_by":1,"name":"Xiaoqing Zhang","email":"","orcid":"","institution":"Ningxia Medical 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Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"Nanozyme, Catalysis, Colorimetric sensor, Glucose detection, CDT antibacterial therapy","lastPublishedDoi":"10.21203/rs.3.rs-9026837/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-9026837/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eRecent years have witnessed growing focus on bimetallic Prussian blue analogs (PBAs) within the cross-disciplinary research areas of materials science and biomedicine. These materials possess a range of favorable properties, including an open framework structure, inherent catalytic activity, excellent biocompatibility, and tunable structural characteristics. Within the current research, a Cu\u0026ndash;Co bimetallic Prussian blue analogue (Cu\u0026ndash;Co PBA) with a characteristic framework structure was effectively fabricated via a straightforward low-temperature co-precipitation approach. Owing to its cost-effectiveness, high stability, straightforward preparation, and the synergistic interaction between Cu\u003csup\u003e2+\u003c/sup\u003e and Co\u003csup\u003e2+\u003c/sup\u003e ions, Cu\u0026ndash;Co PBA shows significant potential as a multifunctional nanozyme and catalytic material. The catalyst demonstrates exceptionally high peroxidase-mimicking activity as a result of the synergistic interaction between the coexisting Cu\u003csup\u003e+\u003c/sup\u003e/Cu\u003csup\u003e2+\u003c/sup\u003e and Co\u003csup\u003e2+\u003c/sup\u003e/Co\u003csup\u003e3+\u003c/sup\u003e redox pairs within the system. This synergy facilitates electron transfer and remarkably boosts the catalytic decomposition efficiency of H₂O₂. Cu\u0026ndash;Co PBA shows catalytic activity several times higher than single Cu-PBA or Co-PBA, thus improving glucose detection sensitivity and accelerating response kinetics, with a linear detection range of 10 \u0026micro;M\u0026ndash;2000 \u0026micro;M and a low limit of detection of 2 \u0026micro;M. Moreover, Cu\u0026ndash;Co PBA nanoparticles achieve efficient chemodynamic antibacterial performance by catalyzing H₂O₂ to produce hydroxyl radicals (\u0026middot;OH), which destroy bacterial cell structures and endow broad-spectrum antimicrobial activity. The inhibition rates for Staphylococcus aureus and Escherichia coli are 99.05% and 98.92%, respectively. 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