The complete mitochondrion genome of Colpomenia sinuosa and phylogenetic analysis

The complete mitochondrion genome of Colpomenia sinuosa and phylogenetic analysis | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article The complete mitochondrion genome of Colpomenia sinuosa and phylogenetic analysis Y iwen Fu, Jinlai Miao, Yuanyuan Zhang This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4604492/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Colpomenia sinuosa plays an important role in marine ecosystem and biological resource utilization. The total length of mitochondrion genome was 39,055 bp in length and consists of a circular DNA molecule. The circular genome is composed of 66 genes, including 38 protein coding genes, 25 tRNA genes, and 3 rRNA genes.The total content of G + C is 32.01%, lower than that of A + T. Phylogenetic analysis confirmed the position of C. sinuosa within Pleurocladia lacustris , which offer valuable insights into the phylogeny and gene functions within the family Stramenopiles and promote novel references for the genetic and phylogenetic research of C. sinuosa through the provision of these data. Colpomenia sinuosa mitochondrion genome phylogenetic analysis Figures Figure 1 Figure 2 1. INTRODUCTION Colpomenia sinuosa belongs to Eukaryota; Sar; Stramenopiles; Ochrophyta; PX clade; Phaeophyceae; Ectocarpales; Scytosiphonaceae; Colpomenia [ 1 ] , and is a representative species of this genus. C. sinuosa is a cosmopolitan brown macroalgal species complex and hence a great candidate for evolutionary studies in the marine environment. Since 2009, three major C. sinuosa phylogenetic lineages, subdivided into eight subgroups, have been identified based on cox3 DNA sequences from worldwide collections. It can adapt to complex intertidal habitats and maintain basal metabolism in extremely harsh environments. The biological characteristics and distribution of C. sinuosa are unique, making it be an excellent object of phylogeography [ 2 ] . In recent years, C. sinuosa has aroused widespread attention and exploration of scientific researchers in the development of marine ecosystems and active substances. 2. MATERIALS AND METHODS The research objects used in this experiment were collected from Sanya Luhuitou (18°14′ N, 109°31′ E) of Hainan Province. These specimens have been meticulously preserved under voucher number FIO2023031209 at the First Institute of Oceanography, Ministry of Natural Resources. ( https://www.fio.org.cn/ , Jinlai Miao, [email protected] ). Shanghai Biozeron Biological Technology Co. Ltd implemented the construction of the whole genome library of C. sinuosa mitochondrion. The genomic paired-end sequencing was carried out on the Illumina NovaSeq 6000 platform. Since the original sequencing data of Illumina had some low-quality data, in order to make the subsequent assembly more accurate, the original data (8124 Mb) were filtered and evaluated. Then, we employed the Illumina NovaSeq 6000 platform for paired-end sequencing [ 3 ] . The mitochondrial genes were meticulously annotated through the utilization of the GeSeq online tool, employing default parameters for the prediction of protein-coding genes, tRNA, and rRNA. The precise positioning of each coding gene was ascertained through thorough BLAST searches against reference mitochondrial genes. NOVOPlasty v4.2 software was utilized to assemble the mitochondrial genome of M. pyrifera [ 4 ] . By enhancing the quality of the original data through quality filtering, we achieved a more accurate assembly, which enabled the prediction of functional genes [ 5 ] . 3. RESULTS AND DISCUSSION The results showed that the mitochondrion genome size was 39,055 bp, and the predicted number of coding genes was 38. The total length of coding genes was 28,317 bp, accounting for 72.51% of the total length. The mitochondrion genome composition was 31.53% A, 36.46% T, 18.03% G, 13.98% C, with the G + C content accounts for 32.01% (Fig. 1 ). Among the coding genes, there were 38 protein-coding genes, 25 tRNA genes and 3 rRNA genes. To further investigate the phylogenetic relationship of C. sinuosa , we used MEGA 7.0 to perform multiple sequence alignment with the chloroplast genomes of 10 species selected from NCBI-GenBank database, and constructed a maximum-likelihood (ML) phylogenetic tree (Fig. 1 ). The nucleotide substitution model is General Time Reversible (GTR, nst = 6) here. The phylogenic result demonstrated that C. sinuosa was closely related with Pleurocladia lacustris (Fig. 2 ) . 4. CONCLUSION In this study, we successfully assembled and analyzed the complete mitochondrial genome of C. sinuosa . This provides valuable genomic data for further research into evolutionary history and phylogenetic relationships among different species of Macrocystis as well as serving as a reference point for the development and utilization of marine algal resources. Declarations DATAAVAILABILITY STATEMENT The genome sequence data that support the findings of this study are openly available in GenBank of NCBI at [ https://www.ncbi.nlm.nih.gov ] under the accession no. PP460011. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA979970, SRR24825412, and SAMN35616936 respectively. FUNDING This study was supported by the National Key Research and Development Program of China (2023YFD2100604), Natural Science Foundation of China (42176130), Basic Scientific Fund for National Public Research Institutes of China (2022Q10), Natural Science Foundation of Shandong (ZR2021MD044), and Jinan “20 new colleges and universities” Funding Project (202228041). Author Contribution Yiwen Fu wrote the main manuscript text . All authors reviewed the manuscript. References Martins NT, Cassano V, Gurgel CFD (2022) Phylogeography of Colpomenia sinuosa (Ectocarpales, Phaeophyceae) along the Brazilian coast[J]. J Phycol 58(4):543–554 Leyton A, Lienqueo ME, Shene C (2020) Macrocystis pyrifera : substrate for the production of bioactive compounds[J]. J Appl Phycol 32(4):2335–2341 Gascuel O (2010) New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0. Syst Biol 59(3):307–321 Kumar S, Stecher G, Tamura K (2016) MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol 33(7):1870–1874 Tillich M, Lehwark P, Pellizzer T et al (2017) GeSeq – versatile and accurate annotation of organelle genomes[J]. Nucleic Acids Res 45(W1):W6–W11 Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4604492","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":320710642,"identity":"5dcd3a99-07a7-4f6f-ac0b-08f3d5e32f7f","order_by":0,"name":"Y iwen Fu","email":"","orcid":"","institution":"Qingdao University of Science and Technology","correspondingAuthor":false,"prefix":"","firstName":"Y","middleName":"iwen","lastName":"Fu","suffix":""},{"id":320710643,"identity":"7be44df0-bb22-48cf-8baa-4e2a0dba9fff","order_by":1,"name":"Jinlai Miao","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA5ElEQVRIiWNgGAWjYDACZiCWMGCQYWBmPvAhgRQtPAzMbIkziNMCBTxAZDiDKKW67cwPH1gU3OHhb+f52PCggkHOvH8B4+cCPFrMDrMZG0gYPOOROMy7sSHhDIOxzI0HzNL47DM7zGAmIWFwmIfhMO/2B4ltDIkzJA6wMfPg1cL+DaxF/jDPwwYitfBAbAEiRogW/gaCWooNQFoMD7MZAv0iYSwhwdgsjVfL+eMbH0v8OSwnd/7ww8YfFTZyEvyHD37GpwUEmCUQbCBTIrGBgAYGBsYPKFz+AwR1jIJRMApGwcgCANQnRNUJH5VIAAAAAElFTkSuQmCC","orcid":"","institution":"Ministry of Natural Resources","correspondingAuthor":true,"prefix":"","firstName":"Jinlai","middleName":"","lastName":"Miao","suffix":""},{"id":320710644,"identity":"0c9929c3-789c-4ce1-9829-08605c673cfc","order_by":2,"name":"Yuanyuan Zhang","email":"","orcid":"","institution":"Qingdao University of Science and Technology","correspondingAuthor":false,"prefix":"","firstName":"Yuanyuan","middleName":"","lastName":"Zhang","suffix":""}],"badges":[],"createdAt":"2024-06-19 08:47:37","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4604492/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4604492/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":60126478,"identity":"1755aaed-c804-404d-b088-f3b88e5b5157","added_by":"auto","created_at":"2024-07-12 06:06:14","extension":"jpg","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":129620,"visible":true,"origin":"","legend":"\u003cp\u003eGenome Map of \u003cem\u003eC. sinuosa\u003c/em\u003e mitochondrial genome (The inner circle indicates the GC content, and the external circle indicates the genes having different colors based on their functions. The different colored legends in the bottom left corner represent genes with different functions).\u003c/p\u003e","description":"","filename":"Figure1.jpg","url":"https://assets-eu.researchsquare.com/files/rs-4604492/v1/2b84157d5eb76b3be75bc995.jpg"},{"id":60126479,"identity":"73f6153a-af01-4a07-92a5-fb24c2c79aaf","added_by":"auto","created_at":"2024-07-12 06:06:14","extension":"jpg","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":172641,"visible":true,"origin":"","legend":"\u003cp\u003ePhylogenetic tree of \u003cem\u003eC. sinuosa\u003c/em\u003e .\u003c/p\u003e","description":"","filename":"Figure2.jpg","url":"https://assets-eu.researchsquare.com/files/rs-4604492/v1/2a94fc80f383edc922df646e.jpg"},{"id":67611850,"identity":"c0d15e6e-23ed-47bf-8147-f8a54a7a2404","added_by":"auto","created_at":"2024-10-28 05:55:48","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":487871,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4604492/v1/362d1785-be7b-4514-8417-d361a7f8b23e.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"The complete mitochondrion genome of Colpomenia sinuosa and phylogenetic analysis","fulltext":[{"header":"1. INTRODUCTION","content":"\u003cp\u003e \u003cem\u003eColpomenia sinuosa\u003c/em\u003e belongs to Eukaryota; Sar; Stramenopiles; Ochrophyta; PX clade; Phaeophyceae; Ectocarpales; Scytosiphonaceae; Colpomenia\u003csup\u003e[\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e]\u003c/sup\u003e, and is a representative species of this genus. \u003cem\u003eC. sinuosa\u003c/em\u003e is a cosmopolitan brown macroalgal species complex and hence a great candidate for evolutionary studies in the marine environment. Since 2009, three major \u003cem\u003eC. sinuosa\u003c/em\u003e phylogenetic lineages, subdivided into eight subgroups, have been identified based on cox3 DNA sequences from worldwide collections. It can adapt to complex intertidal habitats and maintain basal metabolism in extremely harsh environments. The biological characteristics and distribution of \u003cem\u003eC. sinuosa\u003c/em\u003e are unique, making it be an excellent object of phylogeography\u003csup\u003e[\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e]\u003c/sup\u003e. In recent years, \u003cem\u003eC. sinuosa\u003c/em\u003e has aroused widespread attention and exploration of scientific researchers in the development of marine ecosystems and active substances.\u003c/p\u003e"},{"header":"2. MATERIALS AND METHODS","content":"\u003cp\u003eThe research objects used in this experiment were collected from Sanya Luhuitou (18\u0026deg;14\u0026prime; N, 109\u0026deg;31\u0026prime; E) of Hainan Province. These specimens have been meticulously preserved under voucher number FIO2023031209 at the First Institute of Oceanography, Ministry of Natural Resources. (\u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://www.fio.org.cn/\u003c/span\u003e\u003cspan address=\"https://www.fio.org.cn/\" targettype=\"URL\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e, Jinlai Miao, [email protected]).\u003c/p\u003e \u003cp\u003eShanghai Biozeron Biological Technology Co. Ltd implemented the construction of the whole genome library of \u003cem\u003eC. sinuosa\u003c/em\u003e mitochondrion. The genomic paired-end sequencing was carried out on the Illumina NovaSeq 6000 platform. Since the original sequencing data of Illumina had some low-quality data, in order to make the subsequent assembly more accurate, the original data (8124 Mb) were filtered and evaluated. Then, we employed the Illumina NovaSeq 6000 platform for paired-end sequencing\u003csup\u003e[\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e]\u003c/sup\u003e. The mitochondrial genes were meticulously annotated through the utilization of the GeSeq online tool, employing default parameters for the prediction of protein-coding genes, tRNA, and rRNA. The precise positioning of each coding gene was ascertained through thorough BLAST searches against reference mitochondrial genes. NOVOPlasty v4.2 software was utilized to assemble the mitochondrial genome of M. pyrifera\u003csup\u003e[\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e]\u003c/sup\u003e. By enhancing the quality of the original data through quality filtering, we achieved a more accurate assembly, which enabled the prediction of functional genes\u003csup\u003e[\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e]\u003c/sup\u003e.\u003c/p\u003e"},{"header":"3. RESULTS AND DISCUSSION","content":"\u003cp\u003eThe results showed that the mitochondrion genome size was 39,055 bp, and the predicted number of coding genes was 38. The total length of coding genes was 28,317 bp, accounting for 72.51% of the total length. The mitochondrion genome composition was 31.53% A, 36.46% T, 18.03% G, 13.98% C, with the G\u0026thinsp;+\u0026thinsp;C content accounts for 32.01% (Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e). Among the coding genes, there were 38 protein-coding genes, 25 tRNA genes and 3 rRNA genes.\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003eTo further investigate the phylogenetic relationship of \u003cem\u003eC. sinuosa\u003c/em\u003e, we used MEGA 7.0 to perform multiple sequence alignment with the chloroplast genomes of 10 species selected from NCBI-GenBank database, and constructed a maximum-likelihood (ML) phylogenetic tree (Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e). The nucleotide substitution model is General Time Reversible (GTR, nst\u0026thinsp;=\u0026thinsp;6) here. The phylogenic result demonstrated that \u003cem\u003eC. sinuosa\u003c/em\u003e was closely related with \u003cem\u003ePleurocladia lacustris\u003c/em\u003e (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003e) .\u003c/p\u003e \u003cp\u003e \u003c/p\u003e"},{"header":"4. CONCLUSION","content":"\u003cp\u003eIn this study, we successfully assembled and analyzed the complete mitochondrial genome of \u003cem\u003eC. sinuosa\u003c/em\u003e. This provides valuable genomic data for further research into evolutionary history and phylogenetic relationships among different species of Macrocystis as well as serving as a reference point for the development and utilization of marine algal resources.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e \u003ch2\u003eDATAAVAILABILITY STATEMENT\u003c/h2\u003e \u003cp\u003eThe genome sequence data that support the findings of this study are openly available in GenBank of NCBI at [\u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://www.ncbi.nlm.nih.gov\u003c/span\u003e\u003cspan address=\"https://www.ncbi.nlm.nih.gov\" targettype=\"URL\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e] under the accession no. PP460011. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA979970, SRR24825412, and SAMN35616936 respectively.\u003c/p\u003e \u003c/p\u003e\u003ch2\u003eFUNDING\u003c/h2\u003e \u003cp\u003eThis study was supported by the National Key Research and Development Program of China (2023YFD2100604), Natural Science Foundation of China (42176130), Basic Scientific Fund for National Public Research Institutes of China (2022Q10), Natural Science Foundation of Shandong (ZR2021MD044), and Jinan \u0026ldquo;20 new colleges and universities\u0026rdquo; Funding Project (202228041).\u003c/p\u003e\u003ch2\u003eAuthor Contribution\u003c/h2\u003e\u003cp\u003eYiwen Fu wrote the main manuscript text . All authors reviewed the manuscript.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\u003cli\u003e\u003cspan\u003eMartins NT, Cassano V, Gurgel CFD (2022) Phylogeography of \u003cem\u003eColpomenia sinuosa\u003c/em\u003e (Ectocarpales, Phaeophyceae) along the Brazilian coast[J]. J Phycol 58(4):543\u0026ndash;554\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eLeyton A, Lienqueo ME, Shene C (2020) \u003cem\u003eMacrocystis pyrifera\u003c/em\u003e: substrate for the production of bioactive compounds[J]. J Appl Phycol 32(4):2335\u0026ndash;2341\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eGascuel O (2010) New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0. Syst Biol 59(3):307\u0026ndash;321\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eKumar S, Stecher G, Tamura K (2016) MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol 33(7):1870\u0026ndash;1874\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eTillich M, Lehwark P, Pellizzer T et al (2017) GeSeq \u0026ndash; versatile and accurate annotation of organelle genomes[J]. Nucleic Acids Res 45(W1):W6\u0026ndash;W11\u003c/span\u003e\u003c/li\u003e\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Colpomenia sinuosa, mitochondrion genome, phylogenetic analysis","lastPublishedDoi":"10.21203/rs.3.rs-4604492/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4604492/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e \u003cem\u003eColpomenia sinuosa\u003c/em\u003e plays an important role in marine ecosystem and biological resource utilization. The total length of mitochondrion genome was 39,055 bp in length and consists of a circular DNA molecule. The circular genome is composed of 66 genes, including 38 protein coding genes, 25 tRNA genes, and 3 rRNA genes.The total content of G\u0026thinsp;+\u0026thinsp;C is 32.01%, lower than that of A\u0026thinsp;+\u0026thinsp;T. Phylogenetic analysis confirmed the position of \u003cem\u003eC. sinuosa\u003c/em\u003e within \u003cem\u003ePleurocladia lacustris\u003c/em\u003e, which offer valuable insights into the phylogeny and gene functions within the family Stramenopiles and promote novel references for the genetic and phylogenetic research of \u003cem\u003eC. sinuosa\u003c/em\u003e through the provision of these data.\u003c/p\u003e","manuscriptTitle":"The complete mitochondrion genome of Colpomenia sinuosa and phylogenetic analysis","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-07-12 06:06:09","doi":"10.21203/rs.3.rs-4604492/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"8c98f467-0fbb-4845-8938-2ec90f6df99e","owner":[],"postedDate":"July 12th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2024-10-28T05:54:19+00:00","versionOfRecord":[],"versionCreatedAt":"2024-07-12 06:06:09","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-4604492","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4604492","identity":"rs-4604492","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}