Repair effect analysis of mesenchymal stem cell conditioned media from multiple sources on HUVECs damaged by high glucose

Repair effect analysis of mesenchymal stem cell conditioned media from multiple sources on HUVECs damaged by high glucose | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Repair effect analysis of mesenchymal stem cell conditioned media from multiple sources on HUVECs damaged by high glucose Xueyan Guo, Junyan Wang, Rong Su, Dan Luo, Keli Zhao, Yan Li This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4520607/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 30 Dec, 2024 Read the published version in Clinical Proteomics → Version 1 posted 9 You are reading this latest preprint version Abstract Background The therapeutic potential of mesenchymal stem cells (MSCs) may be partly attributed to their secretion growth factors, cytokines and chemokines. In various preclinical studies, the use of MSC-conditioned media (CM) has demonstrated promising potential for promoting vascular repair. Methods To gain a comprehensive understanding of the variations in conditioned media derived from different sources of mesenchymal stem cells (MSCs) including umbilical cord, adipose and bone marrow, we investigated their reparative effects on human umbilical vein endothelial cells (HUVECs) subjected to damage induced by high glucose. Initially, the secreted proteins from the three types of MSCs were assessed using the bicinchoninic acid (BCA) method. Subsequently, we examined the influence of different type of MSC secreted proteins on the proliferation of HUVECs under high glucose conditions. Following this, transwell migration experiments were conducted to evaluate the impact of MSC source on the migration of HUVECs damaged by high glucose. We further compared the effects of adding secreted proteins from the three types of MSCs on the tube formation ability of HUVECs subjected to high glucose damage. Finally, tandem mass tag (TMT) labeling quantitative proteomics was performed to analyze differently expressed proteins in the secreted proteins of three type MSC by using LC-MS/MS. Results In this study, we observed a significantly higher secretion of proteins from umbilical cord mesenchymal stem cells (UMSCs) compared to adipose-derived stem cells (ADSCs). Subsequently, we found that the of proliferation HUVECs was significantly improved with supplementing the three MSCs secreted proteins under high glucose medium. Notably, the reparative effects of bone marrow mesenchymal stem cells (BMSCs) and UMSCs were superior to those of ADSCs. Afterwards, UMSCs exhibited the strongest ability to repair cell migration when HUVECs damaged by high glucose. Moreover, all three MSCs' secreted proteins exhibited the ability to enhance tube formation. Importantly, the UMSCs' secretome showed the most pronounced improvement in tube formation, as evidenced by the evaluation of parameters such as the number of nodes, the number of branches, and total length. These findings suggest that the UMSCs' secretome plays a crucial role in biological processes such as vasculature development, cell adhesion, and tissue remodeling. Additionally, the BMSCs' secretome was found to promote vascular development. The results collectively indicate the diverse therapeutic potential of MSC secretomes in influencing various aspects of cellular function and tissue repair. Conclusion In conclusion, this study offers a valuable reference for the selection of more suitable sources of mesenchymal stem cells (MSCs) in the treatment of diabetic cardiovascular disease. mesenchymal stem cells conditioned media high glucose human umbilical vein endothelial cells repair effect proteomics Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 30 Dec, 2024 Read the published version in Clinical Proteomics → Version 1 posted Editorial decision: Revision requested 19 Sep, 2024 Reviews received at journal 02 Sep, 2024 Reviews received at journal 27 Aug, 2024 Reviewers agreed at journal 21 Aug, 2024 Reviewers agreed at journal 19 Aug, 2024 Reviewers invited by journal 19 Aug, 2024 Editor assigned by journal 03 Jun, 2024 Submission checks completed at journal 03 Jun, 2024 First submitted to journal 03 Jun, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4520607","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":313313238,"identity":"c3b6e40c-1e05-4e7a-bb8d-126ca75d41a9","order_by":0,"name":"Xueyan Guo","email":"","orcid":"","institution":"Foshan Hospital of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine","correspondingAuthor":false,"prefix":"","firstName":"Xueyan","middleName":"","lastName":"Guo","suffix":""},{"id":313313239,"identity":"5c832b87-3e2b-4a2b-b1fd-30c21996b9f2","order_by":1,"name":"Junyan Wang","email":"","orcid":"","institution":"Institute of Biophysics","correspondingAuthor":false,"prefix":"","firstName":"Junyan","middleName":"","lastName":"Wang","suffix":""},{"id":313313242,"identity":"99b5f538-7de0-4537-b6c2-d0aeeb81aaeb","order_by":2,"name":"Rong Su","email":"","orcid":"","institution":"Foshan Hospital of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine","correspondingAuthor":false,"prefix":"","firstName":"Rong","middleName":"","lastName":"Su","suffix":""},{"id":313313245,"identity":"753b9b00-f589-489b-b6c4-3d88ae00fad5","order_by":3,"name":"Dan Luo","email":"","orcid":"","institution":"Western Institute of Health Data Science","correspondingAuthor":false,"prefix":"","firstName":"Dan","middleName":"","lastName":"Luo","suffix":""},{"id":313313246,"identity":"1dfd412c-5b84-4439-9c58-01ed8da3e14d","order_by":4,"name":"Keli Zhao","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAz0lEQVRIie3RsQqCUBTG8SOC0ynXT4h6BUOQhrBXMRxc2loaHAShKWoV7CHyDYTgttTe0OB9g6ZGSaGhIcy2hvufz2/4OEQq1T8GIi2mCZs6/0bAVvIrIbvoSkZZImW6wsA59URJkUdmFrcTbS+c8eEMdo/90CYREG5FO9Hhu5ZcN4RdkFGQDb+dGAgflqzATtKQqgNhLFwrj8G2XhNt3YEAi+U4FWA0W+bbgHH9QkZpmMtNNJ2Zu4so7w9vaKZfyHsG1cedH/oiKpVKpfrUE4vWN4zJq4MXAAAAAElFTkSuQmCC","orcid":"","institution":"Western Institute of Health Data Science","correspondingAuthor":true,"prefix":"","firstName":"Keli","middleName":"","lastName":"Zhao","suffix":""},{"id":313313248,"identity":"af4402e4-7611-4218-9038-f1e5571966c2","order_by":5,"name":"Yan Li","email":"","orcid":"","institution":"Institute of Biophysics","correspondingAuthor":false,"prefix":"","firstName":"Yan","middleName":"","lastName":"Li","suffix":""}],"badges":[],"createdAt":"2024-06-03 09:08:20","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4520607/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4520607/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1186/s12014-024-09521-5","type":"published","date":"2024-12-30T15:57:33+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":73093293,"identity":"00da38f2-4b1b-4fa0-be48-069110992196","added_by":"auto","created_at":"2025-01-06 16:12:59","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":612784,"visible":true,"origin":"","legend":"","description":"","filename":"paper20240603.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4520607/v1_covered_c158bfb8-7184-4d19-9cbf-1c564f81d40d.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Repair effect analysis of mesenchymal stem cell conditioned media from multiple sources on HUVECs damaged by high glucose","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"clinical-proteomics","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"clip","sideBox":"Learn more about [Clinical Proteomics](http://clinicalproteomicsjournal.biomedcentral.com/)","snPcode":"12014","submissionUrl":"https://submission.nature.com/new-submission/12014/3","title":"Clinical Proteomics","twitterHandle":"@BioMedCentral","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"BMC/SO AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"mesenchymal stem cells, conditioned media, high glucose, human umbilical vein endothelial cells, repair effect, proteomics","lastPublishedDoi":"10.21203/rs.3.rs-4520607/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4520607/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eBackground\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eThe therapeutic potential of mesenchymal stem cells (MSCs) may be partly attributed to their secretion growth factors, cytokines and chemokines. In various preclinical studies, the use of MSC-conditioned media (CM) has demonstrated promising potential for promoting vascular repair.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eMethods\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eTo gain a comprehensive understanding of the variations in conditioned media derived from different sources of mesenchymal stem cells (MSCs) including umbilical cord, adipose and bone marrow, we investigated their reparative effects on human umbilical vein endothelial cells (HUVECs) subjected to damage induced by high glucose. Initially, the secreted proteins from the three types of MSCs were assessed using the bicinchoninic acid (BCA) method. Subsequently, we examined the influence of different type of MSC secreted proteins on the proliferation of HUVECs under high glucose conditions. Following this, transwell migration experiments were conducted to evaluate the impact of MSC source on the migration of HUVECs damaged by high glucose. We further compared the effects of adding secreted proteins from the three types of MSCs on the tube formation ability of HUVECs subjected to high glucose damage. Finally, tandem mass tag (TMT) labeling quantitative proteomics was performed to analyze differently expressed proteins in the secreted proteins of three type MSC by using LC-MS/MS.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eResults\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eIn this study, we observed a significantly higher secretion of proteins from umbilical cord mesenchymal stem cells (UMSCs) compared to adipose-derived stem cells (ADSCs). Subsequently, we found that the of proliferation HUVECs was significantly improved with supplementing the three MSCs secreted proteins under high glucose medium. Notably, the reparative effects of bone marrow mesenchymal stem cells (BMSCs) and UMSCs were superior to those of ADSCs. Afterwards, UMSCs exhibited the strongest ability to repair cell migration when HUVECs damaged by high glucose. Moreover, all three MSCs' secreted proteins exhibited the ability to enhance tube formation. Importantly, the UMSCs' secretome showed the most pronounced improvement in tube formation, as evidenced by the evaluation of parameters such as the number of nodes, the number of branches, and total length. These findings suggest that the UMSCs' secretome plays a crucial role in biological processes such as vasculature development, cell adhesion, and tissue remodeling. Additionally, the BMSCs' secretome was found to promote vascular development. The results collectively indicate the diverse therapeutic potential of MSC secretomes in influencing various aspects of cellular function and tissue repair.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eConclusion\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eIn conclusion, this study offers a valuable reference for the selection of more suitable sources of mesenchymal stem cells (MSCs) in the treatment of diabetic cardiovascular disease.\u003c/p\u003e","manuscriptTitle":"Repair effect analysis of mesenchymal stem cell conditioned media from multiple sources on HUVECs damaged by high glucose","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-06-17 05:31:51","doi":"10.21203/rs.3.rs-4520607/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2024-09-19T06:22:30+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2024-09-03T02:38:40+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2024-08-27T20:41:25+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"100170679634676526112080947571939048583","date":"2024-08-21T18:58:41+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"184219175104027720686284487088534382963","date":"2024-08-19T23:46:51+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2024-08-19T23:16:38+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2024-06-03T14:28:41+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2024-06-03T14:28:40+00:00","index":"","fulltext":""},{"type":"submitted","content":"Clinical Proteomics","date":"2024-06-03T09:05:22+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"clinical-proteomics","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"clip","sideBox":"Learn more about [Clinical Proteomics](http://clinicalproteomicsjournal.biomedcentral.com/)","snPcode":"12014","submissionUrl":"https://submission.nature.com/new-submission/12014/3","title":"Clinical Proteomics","twitterHandle":"@BioMedCentral","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"BMC/SO AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"b81e319f-033e-4a30-abf9-610822c82d89","owner":[],"postedDate":"June 17th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[],"tags":[],"updatedAt":"2025-01-06T16:02:22+00:00","versionOfRecord":{"articleIdentity":"rs-4520607","link":"https://doi.org/10.1186/s12014-024-09521-5","journal":{"identity":"clinical-proteomics","isVorOnly":false,"title":"Clinical Proteomics"},"publishedOn":"2024-12-30 15:57:33","publishedOnDateReadable":"December 30th, 2024"},"versionCreatedAt":"2024-06-17 05:31:51","video":"","vorDoi":"10.1186/s12014-024-09521-5","vorDoiUrl":"https://doi.org/10.1186/s12014-024-09521-5","workflowStages":[]},"version":"v1","identity":"rs-4520607","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4520607","identity":"rs-4520607","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}