Green Synthesis and Characterization of Zinc OxideNanoparticles using Iphiona scabra DC. ex Decne Leaf Extract

Green Synthesis and Characterization of Zinc OxideNanoparticles using Iphiona scabra DC. ex Decne Leaf Extract | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Green Synthesis and Characterization of Zinc OxideNanoparticles using Iphiona scabra DC. ex Decne Leaf Extract mahmood saleh, hana AL-Quhbi This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8961552/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 14 You are reading this latest preprint version Abstract Yemeni Iphiona scabra (Y IS ) is widely employed in traditional medicine for the treatment of various diseases and is abundant available in Yemen. In this study, the aqueous leaf extract of Iphiona scabra ( IS .L) was utilized for the green synthesis of zinc oxide nanoparticles (IS-ZnONPs), following a sustainable and environmentally friendly approach. The plant extract acted as both a stabilizing agent and a reducing agent. During synthesis, noticeable color changes were observed in the reaction mixture, shifting from light blue to light brown and finally to dark brown, indicating the successful formation of nanoparticles. The obtained (IS-ZnONPs) were thoroughly characterized vai UV–Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), and X-ray diffraction (XRD). The UV–Vis spectrum revealed a distinct absorption peak at approximately 373 nm for the I. scabra ZnO NPs. Additionally, the band gap energy was found to be 5.6 V. FTIR analysis revealed the functional phytochemical groups responsible for the reduction and capping of the nanoparticles. SEM analysis revealed quasispherical nanoparticles with a homogeneous morphology and a strong tendency to form aggregated clusters, whereas XRD confirmed their crystalline structure and high purity, with an average crystallite size of 14 nm. Green synthesis Iphiona scabra leaves ZnO NPs Full Text Additional Declarations No competing interests reported. Supplementary Files ResponsetoReviewers.pdf Cite Share Download PDF Status: Under Review Version 1 posted Reviews received at journal 18 May, 2026 Reviewers agreed at journal 18 May, 2026 Reviews received at journal 24 Apr, 2026 Reviewers agreed at journal 21 Apr, 2026 Reviewers agreed at journal 20 Apr, 2026 Reviewers agreed at journal 24 Mar, 2026 Reviewers agreed at journal 21 Mar, 2026 Reviewers agreed at journal 20 Mar, 2026 Reviewers agreed at journal 19 Mar, 2026 Reviewers invited by journal 19 Mar, 2026 Editor invited by journal 16 Mar, 2026 Editor assigned by journal 16 Mar, 2026 Submission checks completed at journal 14 Mar, 2026 First submitted to journal 13 Mar, 2026 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8961552","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":609946659,"identity":"992b25bb-1b79-4eb5-8541-d7e14c752423","order_by":0,"name":"mahmood saleh","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA2ElEQVRIiWNgGAWjYFACHhDBzMPG3nzwAQPDAeK1yPHzHEs2IEmLseSMHDMJorTotp89uuFDhXXihgMJZtU8NXfk+BmYHz66gUeL2Zm8tJszzqQDtRxIu81z7JmxZAObsXEOPi0Hcsxu87YdTtxwsOHYbR42IOMAD5s0Xi3n30C1HGZsK+b5R4yWGxBbjCXbmNmYwXoJa3ljBvILMJDZmCXn9gH1NhPyy/kcsxvAEONhk3//8cObb4fl+NmbHz7GpwUFMEHiiFjlIMD4gxTVo2AUjIJRMGIAALmCVIFtpvB6AAAAAElFTkSuQmCC","orcid":"","institution":"University of Aden","correspondingAuthor":true,"prefix":"","firstName":"mahmood","middleName":"","lastName":"saleh","suffix":""},{"id":609946660,"identity":"7f25f131-3863-4efd-b21a-47c40b77d352","order_by":1,"name":"hana AL-Quhbi","email":"","orcid":"","institution":"University of Aden","correspondingAuthor":false,"prefix":"","firstName":"hana","middleName":"","lastName":"AL-Quhbi","suffix":""}],"badges":[],"createdAt":"2026-02-24 23:24:17","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-8961552/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-8961552/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":105564111,"identity":"99f4e349-ae43-4302-98cd-280962788e4a","added_by":"auto","created_at":"2026-03-27 12:48:45","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":945981,"visible":true,"origin":"","legend":"","description":"","filename":"MainManuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-8961552/v1_covered_0ec30765-1d41-48b2-a40e-719ff613814e.pdf"},{"id":105242494,"identity":"3877a730-ecec-498c-b0b9-c4e76508c18b","added_by":"auto","created_at":"2026-03-23 23:31:42","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"supplement","size":279495,"visible":true,"origin":"","legend":"","description":"","filename":"ResponsetoReviewers.pdf","url":"https://assets-eu.researchsquare.com/files/rs-8961552/v1/936d0ab4eae3490dbb37b6a2.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Green Synthesis and Characterization of Zinc OxideNanoparticles using Iphiona scabra DC. ex Decne Leaf Extract","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"discover-applied-sciences","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"","sideBox":"Learn more about [Discover Applied Sciences](https://link.springer.com/journal/42452)","snPcode":"42452","submissionUrl":"https://submission.springernature.com/new-submission/42452/3","title":"Discover Applied Sciences","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Discover Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Green synthesis, Iphiona scabra leaves, ZnO NPs","lastPublishedDoi":"10.21203/rs.3.rs-8961552/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-8961552/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eYemeni \u003cem\u003eIphiona scabra\u003c/em\u003e (Y\u003cem\u003eIS\u003c/em\u003e) is widely employed in traditional medicine for the treatment of various diseases and is abundant available in Yemen. 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