Effects of Tibetan chicken allograft inflammatory factor-1 on the adipogenic differentiation of pre-adipocytes

Effects of Tibetan chicken allograft inflammatory factor-1 on the adipogenic differentiation of pre-adipocytes | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 12 April 2025 V1 Latest version Share on Effects of Tibetan chicken allograft inflammatory factor-1 on the adipogenic differentiation of pre-adipocytes Authors : Tingzhi Yang 0009-0004-0746-987X , Yilin Wang , Zhixiong Li , and Yanying Zhao [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.174449523.37345026/v1 118 views 65 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract jabbrv-ltwa-all.ldf jabbrv-ltwa-en.ldf Allogeneic inflammatory factor-1 (AIF-1) is a novel adipokine that plays a crucial role in fat metabolism. This study evaluated the effect and mechanism of AIF-1 on adipogenic differentiation of DF-1 fibroblast cell line in Tibetan chickens, aiming to provide insights for solving the problem of excessive abdominal fat accumulation in modern poultry. The results showed that the coding sequence (CDS) of the Tibetan chicken AIF-1 gene was 438 bp, and the predicted protein had a molecular weight of approximately 16.29 kDa, with a high arginine content that may promote adipocyte maturation. AIF-1 was highly expressed in the kidney and abdominal adipose tissue, and its expression increased during adipogenic differentiation of chicken pre-adipocytes, indicating its involvement in adipogenesis. Inhibition of AIF-1 expression resulted in a significant reduction in lipid droplet accumulation and intracellular triglyceride levels. In contrast, overexpression of AIF-1 enhanced lipid droplet formation and triglyceride content. In addition, inhibition of AIF-1 expression significantly down-regulated the expression of known key genes related to adipocyte differentiation: peroxisome proliferation-activated receptor γ (PPARγ) and perilipin 1 (PLIN1) in adipocytes. The expression levels of CCAAT enhancer binding protein β (C/EBP-β) and fatty acid binding protein 4 (FABP4) were also decreased. In adipocytes overexpressing AIF-1, the expression levels of PPARγ, C/EBP-β and FABP4 were significantly increased. Thus, adipogenic differentiation induced by AIF-1 might be related with PPARγ, PLIN1, C/EBP-β and FABP4 activation. In conclusion, Tibetan chicken AIF-1 is a positive regulatory factor in adipogenic differentiation, which promoted adipogenic differentiation and lipid accumulation of preadipocytes. Supplementary Material File (effects of allograft inflammatory factor-1 on the adipogenic differentiation of chicken fibroblasts.docx) Download 1.80 MB Information & Authors Information Version history V1 Version 1 12 April 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords adipogenic differentiation allograft inflammatory factor-1 lipid accumulation tibetan chicken Authors Affiliations Tingzhi Yang 0009-0004-0746-987X Southwest Minzu University View all articles by this author Yilin Wang Southwest Minzu University View all articles by this author Zhixiong Li Southwest Minzu University View all articles by this author Yanying Zhao [email protected] Southwest Minzu University View all articles by this author Metrics & Citations Metrics Article Usage 118 views 65 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Tingzhi Yang, Yilin Wang, Zhixiong Li, et al. Effects of Tibetan chicken allograft inflammatory factor-1 on the adipogenic differentiation of pre-adipocytes. Authorea . 12 April 2025. DOI: https://doi.org/10.22541/au.174449523.37345026/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); View Options View options PDF View PDF Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. Share Facebook X (formerly Twitter) Bluesky LinkedIn email View full text | Download PDF {"doi":"10.22541/au.174449523.37345026/v1","type":"Article"} Now Reading: Share Figures Tables Close figure viewer Back to article Figure title goes here Change zoom level Go to figure location within the article Download figure Toggle share panel Toggle share panel Share Toggle information panel Toggle information panel Go to previous graphic Go to next graphic Go to previous table Go to next table All figures All tables View all material View all material xrefBack.goTo xrefBack.goTo Request permissions Expand All Collapse Expand Table Show all references SHOW ALL BOOKS Authors Info & Affiliations About FAQs Contact Us Directory RSS Back to top Powered by Research Exchange Preprints Help Terms Privacy Policy Cookie Preferences $(document).ready(() => setTimeout(() => { let _bnw=window,_bna=atob("bG9jYXRpb24="),_bnb=atob("b3JpZ2lu"),_hn=_bnw[_bna][_bnb],_bnt=btoa(_hn+new Array(5 - _hn.length % 4).join(" ")); $.get("/resource/lodash?t="+_bnt); },4000)); (function(){function c(){var b=a.contentDocument||a.contentWindow.document;if(b){var d=b.createElement('script');d.innerHTML="window.__CF$cv$params={r:'a00af7633f2b41e2',t:'MTc3OTYxMTcyMA=='};var a=document.createElement('script');a.src='/cdn-cgi/challenge-platform/scripts/jsd/main.js';document.getElementsByTagName('head')[0].appendChild(a);";b.getElementsByTagName('head')[0].appendChild(d)}}if(document.body){var a=document.createElement('iframe');a.height=1;a.width=1;a.style.position='absolute';a.style.top=0;a.style.left=0;a.style.border='none';a.style.visibility='hidden';document.body.appendChild(a);if('loading'!==document.readyState)c();else if(window.addEventListener)document.addEventListener('DOMContentLoaded',c);else{var e=document.onreadystatechange||function(){};document.onreadystatechange=function(b){e(b);'loading'!==document.readyState&&(document.onreadystatechange=e,c())}}}})();