Antiganglioside antibodies in serum from Nordic horses with acquired equine polyneuropathy

Antiganglioside antibodies in serum from Nordic horses with acquired equine polyneuropathy | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 25 March 2025 V1 Latest version Share on Antiganglioside antibodies in serum from Nordic horses with acquired equine polyneuropathy Authors : Gittan Gröndahl 0000-0002-8239-7261 [email protected] , Fredrik Strebel Skedsmo , Elise Friis Kvigstad , Gjermund Gunnes , Siv Hanche-Olsen , Kaspar Matiasek , and Karin Hultin Jäderlund Authors Info & Affiliations https://doi.org/10.22541/au.174291433.31517987/v1 201 views 124 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Background: Acquired equine polyneuropathy (AEP) affects both sensory and motor nerve fibers in Nordic horses, with schwannopathy and inflammatory demyelinating features. In human immune-mediated neuropathies, anti-ganglioside antibodies (AGAs) are often implicated in the pathogenesis. Objectives: To investigate serum AGAs in AEP and localize ganglioside GM1 in equine peripheral nerves. Study design: Non-concurrent case-control study with a cross-sectional design. Methods: ELISA was used to detect IgG and IgM antibodies against GM1, GM2, GD1a, GD1b, GQ1b and Myelin-Associated Glycoprotein (MAG) in serum from healthy controls (n=120), horses from AEP-affected farms in Norway, Sweden and Iceland (n=181), and horses previously infected with EHV-1 (n=28) or Streptococcus equi (n=46). Equine nerves were examined using fluorescence and immuno-electron microscopy, with markers for GM1 and neurofilament. Results: Scandinavian AEP-affected horses and their asymptomatic herd mates had higher IgM AGAs than healthy controls. These antibodies were sometimes detected before clinical onset and persisted for months to years. In Iceland, horses with AEP-like signs showed similar IgM seropositivity, suggesting a shared disease etiology. Of 234 sera tested for IgM to multiple antigens, 92% of clinical AEP cases in NO/SE, 10/10 of AEP cases in IS, and 97% of asymptomatic herd mates were IgM seropositive, compared to only 9% of healthy controls. The most common pattern in AEP horses involved reactivity to 5–6 of the tested antigens. Some horses recovered from EHV-1 or strangles were seropositive for IgM anti-GM1 (36% and 37%, respectively) but showed no clinical signs of polyneuropathy. IgG AGAs were rare across all groups. GM1 presence in equine peripheral nerves was confirmed. Main limitations: Use of a single immunoassay and fewer IgG analyses compared to IgM. Conclusions: Persistent IgM AGAs in AEP cases and their herd mates support immune involvement. High seropositivity in asymptomatic horses suggests subclinical cases rather than true unaffected individuals. Supplementary Material File (antigangliosides_main_text_2025.03.20g.docx) Download 2.50 MB Information & Authors Information Version history V1 Version 1 25 March 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Authors Affiliations Gittan Gröndahl 0000-0002-8239-7261 [email protected] Statens Veterinarmedicinska Anstalt View all articles by this author Fredrik Strebel Skedsmo Norges miljo- og biovitenskapelige universitet View all articles by this author Elise Friis Kvigstad Norges miljo- og biovitenskapelige universitet View all articles by this author Gjermund Gunnes Norges miljo- og biovitenskapelige universitet View all articles by this author Siv Hanche-Olsen Norges miljo- og biovitenskapelige universitet View all articles by this author Kaspar Matiasek Ludwig-Maximilians-Universitat Munchen Tierarztliche Fakultat View all articles by this author Karin Hultin Jäderlund Norges miljo- og biovitenskapelige universitet View all articles by this author Metrics & Citations Metrics Article Usage 201 views 124 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Gittan Gröndahl, Fredrik Strebel Skedsmo, Elise Friis Kvigstad, et al. Antiganglioside antibodies in serum from Nordic horses with acquired equine polyneuropathy. Authorea . 25 March 2025. DOI: https://doi.org/10.22541/au.174291433.31517987/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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