Virus-Specific  Alterations in Placental Structure and Transporter Expression Following  Maternal Arbovirus Infection            

Virus-Specific Alterations in Placental Structure and Transporter Expression Following Maternal Arbovirus Infection | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 17 December 2025 V2 Latest version Share on Virus-Specific Alterations in Placental Structure and Transporter Expression Following Maternal Arbovirus Infection Authors : Cherley B.V. Andrade 0000-0002-4320-4252 , Enrrico Bloise , Natália L. Silva , Jair R.S. Braga , Sharton Vinicius Coelho 0000-0003-0794-7160 , Guinever E. Imperio , Camila O.S. Nunes , Antonio Jose Cunha , Luciana B. Arruda , Arnaldo Prata-Barbosa , Kristin L. Connor , and Tania M. Ortiga-Carvalho [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.176249358.83389670/v2 228 views 143 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Gestational arboviral infections may compromise fetal brain development. We hypothesized that arboviral infections during pregnancy impair placental histomorphometry and key transporter systems implicated with fetal brain development. Placental samples (n=54) were collected from singleton pregnancies affected by chikungunya (CHIKV; n=14), dengue (DENV; n=10), zika (ZIKV; n=15), or healthy controls (HC, n=15). Histomorphometry and immunohistochemistry were performed, with quantification of xenobiotic, nutrient, thyroid hormone transporters, and cell turnover markers. Data were analyzed using ANOVA (p<0.05) and principal component analysis (PCA). CHIKV was associated with reduced syncytiotrophoblast and syncytial knot (SK) areas compared with HC, and reduced SK area versus ZIKV. DENV showed decreased SK area versus HC and ZIKV, reduced connective tissue area compared with HC and CHIKV, and increased fetal blood vessel (FBV) area versus HC. ZIKV was linked to reduced syncytiotrophoblast and increased FBV area versus HC. All arboviral infections showed reduced immunoreactive (ir) expression of the protective transporters P-gp and BCRP. Nutrient transporter expression was altered across groups: CHIKV exhibited reduced ABCA1, SNAT2, and GLUT1; DENV exhibited reduced ABCA1 and SNAT2, whereas ZIKV exhibited reduced ABCA1, SNAT2 and GLUT1 with increased SNAT4 versus HC. MCT8 and Ki67 ir-expression were elevated in CHIKV and ZIKV compared with HC (all p<0.05). PCA distinguished infected from uninfected groups based on placental functional markers related to fetal brain development, but not on histomorphometry. Arboviral-infected placentae showed distinct structural changes and reduced nutrient, hormone, and xenobiotic transporter expression, potentially increasing fetal brain vulnerability and long-term health risks even when neonatal symptoms are minimal. Supplementary Material File (table 1.docx) Download 17.54 KB File (virus-specific alterations in placental structure and transporter expression following maternal arbovirus infection.docx) Download 2.28 MB Information & Authors Information Version history V1 Version 1 07 November 2025 V2 Version 2 17 December 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords arboviruses fetal brain development nutrient transport thyroid hormone transporter trophoblast xenobiotic transporter Authors Affiliations Cherley B.V. Andrade 0000-0002-4320-4252 Universidade Federal do Rio de Janeiro Instituto de Biofisica Carlos Chagas Filho View all articles by this author Enrrico Bloise Universidade Federal de Minas Gerais View all articles by this author Natália L. Silva Universidade Federal do Rio de Janeiro Instituto de Biofisica Carlos Chagas Filho View all articles by this author Jair R.S. Braga Universidade Federal do Rio de Janeiro Instituto de Biofisica Carlos Chagas Filho View all articles by this author Sharton Vinicius Coelho 0000-0003-0794-7160 Universidade Federal do Rio de Janeiro Instituto de Microbiologia Professor Paulo de Goes View all articles by this author Guinever E. Imperio Universidade Federal do Rio de Janeiro Instituto de Biofisica Carlos Chagas Filho View all articles by this author Camila O.S. Nunes Universidade Federal do Rio de Janeiro Instituto de Biofisica Carlos Chagas Filho View all articles by this author Antonio Jose Cunha Universidade Federal do Rio de Janeiro Faculdade de Medicina View all articles by this author Luciana B. Arruda Universidade Federal do Rio de Janeiro Instituto de Microbiologia Professor Paulo de Goes View all articles by this author Arnaldo Prata-Barbosa Instituto D'Or de Pesquisa e Ensino View all articles by this author Kristin L. Connor Carleton University Department of Health Sciences View all articles by this author Tania M. Ortiga-Carvalho [email protected] Universidade Federal do Rio de Janeiro Instituto de Biofisica Carlos Chagas Filho View all articles by this author Metrics & Citations Metrics Article Usage 228 views 143 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Cherley B.V. Andrade, Enrrico Bloise, Natália L. Silva, et al. Virus-Specific Alterations in Placental Structure and Transporter Expression Following Maternal Arbovirus Infection . Authorea . 17 December 2025. DOI: https://doi.org/10.22541/au.176249358.83389670/v2 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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