Yield Improvement of Gracilaria tentuistipitata by Optimizing Different aspects in Coast of Cox’s Bazar, Bangladesh

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M. Bokhtair, M. A. Ali, M . A. Z. Chowdhury, K. U. Ahmed, M. K. Hassan, and 5 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-567666/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 9 You are reading this latest preprint version Abstract This research was designed to find out the effect of different factors such as influence of lunar cycle, harvesting interval, rope type and seeding gap on the production of G. tenuistipitata in coast of Cox’s Bazar. Duration of these experiments were sixty days and all the parameters were recorded fortnightly. Monitoring of water quality parameters indicated that salinity, temperature, transparency, pH and DO were suitable for seaweed cultivation. In determining lunar cycle effect, results envisaged that fresh yield was 14.43% increased when seeding and harvesting time was selected considering the moon cycle. Regarding the selection of harvesting interval, it was found that T 3 (30 days interval) was the best to harvest the seaweed whereas T 4 (40 days interval) showed decreasing trend in production. Our study also found that semi floating single line showed better yield performance compared to semi floating double line system. In case of influence on seeding gap, it has been found that 20 cm gap between two seed showed the highest yields followed by 10 cm, 30 cm and 40 cm, respectively. Overall, it can be concluded that yield of G. tenuistipitata in coast of Cox’s Bazar could be improved considering those factors. Plant Physiology and Morphology Agroecology Oceanography Seaweed Gracilaria tenuistipitata Cox’s Bazar Bangladesh Figures Figure 1 Figure 2 Introduction Seaweed is a colloquial term for the common name of mostly macroscopic and multicellular marine algae, which do not have root systems or flowers, leaves, stems, fruits and seeds and generally grow and live attached to rock or other hard substrata below the high-water mark or remain drifted in the oceans[ 1 – 4 ]. Seaweeds is considered as a high profile commercial marine biota for its variety of uses, like raw materials of bio-chemicals (agar, agarose, algin, and carrageenan), dyes, food, feed, enzymes and drugs [ 5 ]. Bangladesh is thought to be in the transitional zone for the flora and fauna of the Indian subcontinent and Southeast Asia and is part of the Indo-Burma biodiversity hotspot [ 6 ]. It has 710-km-long coastline and a 25,000-sq-km coastal area which is supported by a variety of land use practices. The seacoast of Bangladesh is considered as one of the unreached areas of the world in the field of phycology. This coastal area, with both sandy and muddy beaches, estuaries and mangrove swamps, provides substrates and habitats for the cultivation of various kinds of seaweeds, according to experts. Gracilaria species are notable for their economic importance as various products like agar, human food, animal feed, fertilizer, drugs, and biofuel are produced from it [ 7 ]. With a cosmopolitan distribution and high growth rates, this species is considered as good candidates for cultivation in various parts of the world [ 8 ]. Seaweed culture in Bangladesh is still in an initial stage. However, a few researchers are involved in seaweed cultivation in the south-eastern and south-western coasts of Bangladesh and the methods of cultivation of seaweeds using indigenous materials like bamboo and rope [ 6 ]. There are a few number of experiment conducted on culture of seaweed in Bangladesh where all of them either focused on the adaptation of culture technique based on environmental parameters or identifying potential sites for seaweed [ 9 – 11 ]. No experiment conducted regarding other possible factors which has influence on the yield performance of the seaweed culture in Bangladesh. Harrison and Hurd [ 12 ] reported that success in cultivating seaweed depends on both of the knowledge of ecophysiological characteristics of the species and on the important factors for seaweed growth that are responsible for better yields performance. Hence, the aim of this study was to observe the effect of some crucial factors such as lunar cycle, harvesting interval, planting system and seeding distance on the production of seaweed Gracilaria tenuistipitata in Nuniarchara coast, Cox’s Bazar. Results Water Quality Parameters Seaweed culture needs suitable physico-chemical parameters. The mean (±SD) values of different water quality parameters during the experimental period are presented in Table 1. Table 1. Mean values of water quality parameters during experimental period of 60 days Average water quality parameters Parameters Salinity (ppt) Temp. ( o C) Transparency (cm) pH DO (mg/L) 32±0.3 22±0.1 74.5±1.4 8.0±0.1 7.2±0.1 Nutrient parameters Nutrient parameters of water and soil in the seaweed beds of Nuniachara coast of Cox’s Bazar are presented in the Table 2. Table 2. Water and soil nutrients (mean±Sd) of seaweed bed in Nuniachara coast of Cox’s Bazar Water Parameters Unit Nitrate (NO 3 ) (mg/L) 0.632±0.2 Nitrite (NO 2 ) (mg/L) 0.443±0.11 Sulphate (S) (mg/L) 10,234.25±125.3 Calcium (Ca) (ppm) 437.30±7.1 Soil parameters Soil calcium (Ca) (ppm) 16,387.32±103.5 Effect of lunar cycle on the yields of Gracilaria tenuistipitata In this experiment in T 0, seeds of seaweeds were transplanted three days after full moon and harvested three days before next full moon. On the other hand in T 1, seaweeds were harvested and transplanted in between two consecutive full moons. It is found that, full moon has impact on the yield performance of Gracilaria tenuistipitata (Table 3) . Table 3. Effect of lunar cycle on the yield performance of Gracilaria tenuistipitata Treatments Full moon during experiment Fresh yield (t ha -1 ) Dry yield (t ha -1 ) T 0 12 December and 11 January 9.12 ± 0.66 1.55 ±0.19 T 1 7.97± 0.80 1.35±0.14 ∆T= T 0- T 1 1.15 0.20 % increase 14.43 14.81 The yields found higher in T 0 (where seeds were transplanted after full moon and harvested before the next full moon) than T 1 (in between two consecutive full moons). Effect of harvesting interval on the yields of Gracilaria tenuistipitata In this case, the impact of harvesting interval (10, 20, 30, 40 days) on the yield performance of Gracillaria tenuistipitata presented in figure 1. It is found that the production increased in T 1 , T 2 and in T 3 where the yields were highest in T 3 and the production decreased in T 4. These results indicate that 30 days of interval are the peak period to harvest the seaweed. Effect of planting system on the yield Two methods of planting system were followed to see the yield performance of Gracilaria species. It is found that the yield performance is better in semi floating single line (T 1 ) than semi floating double line (T 2 ) (Table 4 and 5). Table 4 A. Yield Performance of Gracilaria t enuistipitata in semi floating single line (T 1 ) No. of row Fresh weight (ton/ha) Dry weight (ton/ha) 1 12.78 2.03 2 13.63 2.18 3 11.99 1.92 4 13.52 2.16 5 11.79 1.87 Mean ± SD 12.74 ±0.85 A 2.03 ±0.14 A Table 4B. Yield Performance of Gracilaria t enuistipitata species in semi floating double line (T 2 ) No. of row Fresh weight (ton/ha) Dry weight (ton/ha) 1 7.49 1.29 2 6.92 1.15 3 7.91 1.19 4 6.75 1.09 5 7.31 1.24 Mean ± SD 7.28±0.46 B 1.19 ±0.08 B Table 5. Yield Performance of Gracilaria tenuistipitata species in semi floating double line (T 2 ) The fresh yield of in semi floating single line was 12.74±0.8 t ha -1 that was significantly higher than the yield in semi floating double line system (7.28±0.46 t ha -1 ). The 20 cm length of planting distance showed significantly higher amount of yields (2.30 t ha -1 ) than the other three treatments. T 4 which denotes the planting distance 40 cm showed the lowest yield performance (1.5 t ha -1 ). Effect of seeding distance on the yields of Gracilaria tenuistipitata The yield of Gracilaria tenuistipitata seedlings in each treatments during the cultivation periods (30 days) is graphed in figure 2. It shows that the planting distances influence the yields of Gracilaria tenuistipitata (p<0.05). Discussion Seaweeds are not only source of food, feed and medicine but also a source of bioactive compounds which has nutritional and biomedical applications [ 13 ]. In recent years, seaweed cultivation has been an issue of much importance all over the world. Gracilaria tenuistipitata is an important seaweed species which has tolerant to a wide range of environments and an economically important raw material for agar production [ 14 ]. Most of the researches on seaweed farming is limited to searching the suitable location, selection of farming method and suitability of seaweed seed [ 10 , 11 , 15 ]. The success of seaweed farming does not only depend on ecological suitability of the site, but also to a large extent on some factors that also have impact on the production performance of seaweed. In this study, we evaluated influences of some other factors like effect of lunar cycle, rope type, harvesting interval and seeding gap on the improvement of production performances. In these experiments, the water quality parameters and nutrient parameters of soil and water can be said suitable for seaweed culture according to other research findings [ 16 – 18 ]. Gracilaria has a high tolerance of environmental parameters [ 19 – 21 ]. The optimum environment could improve nutrient absorption process so that it could improve the growth rate of seaweeds.[ 10 , 22 , 23 ] Regarding the effect of lunar cycle, seeding and harvesting was carried considering lunar cycle as tidal effect showed strong influence on the productivity of seaweed farming. Results showed that full moon has clear influential effect on the yield of Gracilaria tenuistipitata since during the day of full moon and three days before and after the full moon, the wave pressure remains higher than usual. Mulford [ 24 ] reported that when the moon is full, this causes a stronger pull-on ocean as the Earth and Moon are aligned, which causes a more pronounced tide. In present study, reduced yield performance recorded when seaweeds were harvested and transplanted in between two consecutive full moons. On the other hand, better yield performance recorded when the seeds were transplanted three days after full moon and harvested three days before next full moon. This was happened because during full moon in the middle or late of cultivation cycle, highest high tide and waves occur. Stronger high tide reduced the yield by storming off the long crop rope of Gracilaria tenuistipitata. Yield may be increased by 10 to 15 percent if we seed the crop three days before the full moon and harvest it three days after the next full moon (24 days field duration), instead of seeding at first day of the month and harvesting at last day (30 days field duration). Thus, we can save 6 days which can be used to dry and processing the harvest and also to take preparations for the next seeding. It has been found that harvesting duration also another factor which may influence on the productivity of seaweed farming. Timely harvesting of any crops ensures good crop quality and high market value. Another experiment also carried out to search out the suitable harvesting interval for obtaining better yield. In our present study, it is found that harvesting seaweeds after 30 days of seeding showed maximum yield performance. Reduced yield performance found when seaweeds were harvested after 40 days of seeding. The reason behind this could be rotting of the bunch of ropes of seaweed after 30 days of period and loss of the yield of seaweed has been occurred. Although no research findings is available in the literature regarding this factor, our study revealed that 30 days of harvesting interval is the peak period to harvest. Harvesting needs to be carried out in such a way as to maintain peak productivity. Rope type has significant impact on the yield performance of the Gracilaria tenuistipitata. In our study, it has been seen that semi floating single line rope method was found better over semi-floating double line rope method and the yield was significantly higher in semi floating single line rope method. The higher yield was found due to fact that seeds of double line floating system were more submerged than the single line floating system and it happened to decompose the seeds of seaweeds and reduced the yield. Additionally, penetration of sunlight was less due to double line floating system which ultimately hampers the photosynthesis. Most of the researchers mainly focused on methodological approaches and main cultivation methods and discusses different problems arising during the application of these methods [ 25 , 26 ] but no experiment found to be conducted regarding the planting method on the yield performance of Gracilaria tenuistipitata. Seeding gap is a crucial factor for seaweed production. Neish [ 27 ] reported that the suitable planting distance will provide wider water circulation which take nutrients leading to enhance diffusion process in upgrading the metabolic and growth rate. In our present study, 20 cm length of seeding gap resulted significantly higher amount of yields. In this study, the 20cm-seeding gap is assumed to be suitable for receiving the intensity of sunlight in all thalli parts of Gracilaria , while in the other planting distance (10,30 & 40 cm) might be too narrow or too wide that indicates thalli’s surface cover each other and also covered by filamentous epiphyte algae that impede sunlight absorption. These findings are in line with a report of Reddy et al. [ 28 ] and Aslin et al. [ 29 ] who found similar result in their experiment. Taking all those factors into account, these studies revealed that Gracilaria tenuistipitata species could be cultured in our coast particularly in Nuniachara coast of Cox’s Bazar. However, for getting maximum production, some effects such as lunar cycle, rope type, harvesting intervals of the seaweed and seeding gap need to be considered as our studies finds that there are influential impact of this factors on the yield performance of Gracilaria species. So, further research is needed to find out the effect of various factors on the yield performance of seaweed. Methods Study area: Study was conducted from December 2019 to January 2020 in Nuniachara of Cox’s Bazar sadar. The cultivation of seaweeds in open sea is being carried out using one-step seed production method which requires healthy seaweeds. Taking the above considerations into account, experiment site has been primarily selected at Nuniachara coast of Cox’s Bazar sadar. Collection of seaweed seed: Wild G. tenuistipitata as seed was collected from Saint Martin’s Island (92º28ʹ40.12ʺE and 20º65ʹ51.43ʺN) of Bay of Bengal of Bangladesh. Seed collection from seabed was permitted from the local government and authenticated the botanical identification of seaweed species as the voucher specimen has been previously deposited at BFRI herbarium. Production of seeds: The farming/cultivation of seaweeds in open sea was carried out using one-step seed production method. Seeds for seaweed farming was produced by one-step (directly using cut pieces and tied with the ropes) and multi-step (inducing spore release by a particular seaweed in the green house and allowing spores to grow on ropes and nets) methods. Seaweed culture method: The seeded ropes were arranged in single or double lines in the open sea. Each line was kept attached with two bamboo poles so that it could not be washed away. Plastic floats @ 5 pieces per 25 m long rope were used to keep the ropes moving up and downwards during tides. No fertilizer, insecticides and pesticides were used during seaweed cultivation. Water parameters: Water temperature, salinity, transparency, pH and dissolved oxygen (DO) were checked every 15 days at the cultivation sites. Transparency was measured using Secci disc. A digital DO meter (HACH, USA) was used to determine the dissolved oxygen content of water. pH was measured using a digital pH meter (HACH, USA). Salinity was determined using refractometer. Collection and analysis of soil: Soil samples from the sites were collected and after collection, samples were brought back to the laboratory within 6 h for analysis. In the laboratory, the samples were dried and powdered, sieved and kept in a desiccator until further analysis. Optimization of different factors Experiment 1: Effect of lunar cycle This study was conducted to find out the effect of lunar cycle on the yield performance of Gracilaria tenuistipitata. In this experiment, T 0 indicates control where the seeds were transplanted three days after full moon and harvested three days before next full moon. On the other hand, T 1 indicates that seaweeds were harvested and transplanted in between two consecutive full moons where one highest high tide occurred. Experiment 2: Effect of harvesting interval In this experiment, different harvesting intervals (T 1 = 10 days, T 2 = 20 days, T 3 = 30 days, T 4 = 40 days) were taken to determine the best harvesting time with Gracilaria tenuistipitata by harvesting after 10 days, 20 days, 30 days and 40 days of seeding, respectively for getting maximum production. Experiment 3: Effect of rope type Two types of rope (where T 1 denotes semi-floating single line method and T 2 denotes semi-floating double line method) were followed to evaluate the effect of this method on the yield performance of Gracilaria tenuistipitata. Experiment 4:Effect of seeding gap This experiment was carried out for determining the effect of seeding gap where T 1 ,T 2 ,T 3 and T 4 indicates 10 cm, 20 cm, 30 cm and 40 cm seeding gap, respectively on the yield performance of Gracilaria tenuistipitata. Statistical analysis: Data were statistically analyzed by statistical package SPSS version 16.0 (SPSS Inc., Chicago, IL, USA). Before all analysis data were analyzed for normality by probability plots and for homogeneity of variances by Levene's test. One way ANOVA was used to determine the significance of each parameter among different treatments. If a main effect was significant, the ANOVA was followed by Tukey's test. Level of significance was made at 95% probability level. Declarations Acknowledgements: This research was funded by Krishi Gobeshona Foundation (KGF), Ministry of Agriculture, Bangladesh. Author’s Contribution: Bokhtiar, Ali, Chowdhury, Ahmed designed and executed this research. Hassan and Ahmed reviewed this manuscript. Bhuiyan, Mashuk, and Rahman performed research design, data compilation, manuscript preparation and draft manuscript preparation. Rafiquzzaman supervised research activities. References Chapman, V. J. Seaweeds and Their Uses. Soil Sci. 74 ( 6 ), 299 (1973). Okazaki, A. Seaweeds and Their Uses in Japan; Tokai University Press: [Tokyo], (1971). Round, F. E. 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Bhuiyan","email":"","orcid":"","institution":"Bangladesh Agricultural Research Institute","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"M.","middleName":"S.","lastName":"Bhuiyan","suffix":""},{"id":31340981,"identity":"78dccf92-e172-4038-99d6-77a24c05b55a","order_by":7,"name":"O. F. Mashuk","email":"","orcid":"","institution":"Bangladesh Agricultural Research Institute","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"O.","middleName":"F.","lastName":"Mashuk","suffix":""},{"id":31340982,"identity":"d2287bc9-1467-4c3a-93a6-767cdd0af655","order_by":8,"name":"M M Rahman","email":"","orcid":"","institution":"Bangladesh Agricultural Research Institute","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"M","middleName":"M","lastName":"Rahman","suffix":""},{"id":31340983,"identity":"66f5ed15-6f00-4c3a-a96c-47142631636d","order_by":9,"name":"S. M. Rafiquzzaman","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABDUlEQVRIiWNgGAWjYFACxgcH4OyPDTAWGwODAUMCDi3MBnAtjDOJ1YJg8hKjxbz9MOOBt2119vzTDj97bLujLtq8/YwBw4eywwzm7Ni1yJxJZjg4t42NWeJ2mrlx7pnDuXPO5Bgwzjh3mMGy5wFWLRIM+QcO87bxsDHcTjCTzm07kDuDIceAmbftMIPBDey2SPA/ZgBqkeCRv53+TdqyrS53Bv8bA+a/+LRIJIO0GEgY3M4xk2ZsY86dIQG0hRGvlscMB+ecSzAwvJ1TJtkL9MsMiWcFB3vOpfPg9At/MvOHN2V19nK307dJ/NwBcljyxgc/yqzlcIUYGPCgCxzAJohfyygYBaNgFIwCZAAAErZfBOjNDOAAAAAASUVORK5CYII=","orcid":"","institution":"Bangabandhu Sheikh Mujibur Rahman Agricultural University","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"S.","middleName":"M.","lastName":"Rafiquzzaman","suffix":""}],"badges":[],"createdAt":"2021-05-27 14:14:10","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-567666/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-567666/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":10093519,"identity":"ec2daefc-23fb-4cd1-9ca2-e4a82179c2a6","added_by":"auto","created_at":"2021-06-07 21:42:47","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":7884,"visible":true,"origin":"","legend":"Effect of harvesting interval on the yields of Gracilllaria tenuistipitata","description":"","filename":"drawingimage1.png","url":"https://assets-eu.researchsquare.com/files/rs-567666/v1/b00735d2ab3077b84750fc01.png"},{"id":10093769,"identity":"ede5dcdb-6934-4046-8bd7-31fed8ece8aa","added_by":"auto","created_at":"2021-06-07 21:46:05","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":5214,"visible":true,"origin":"","legend":"Dry yields in different seeding distance. Bars with different letters were significantly (p\u003c0.05) different from each other","description":"","filename":"drawingimage2.png","url":"https://assets-eu.researchsquare.com/files/rs-567666/v1/c4df3ca4c6a85920a1aa21cf.png"},{"id":15673415,"identity":"aaa36b46-99d4-4d3f-a802-d4b6533565b6","added_by":"auto","created_at":"2021-11-18 14:17:51","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":478303,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-567666/v1/e9049bd0-e4d0-4f02-9d6d-dbbd33db2e6c.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"\u003cp\u003eYield Improvement of \u003cem\u003eGracilaria tentuistipitata \u003c/em\u003eby Optimizing Different aspects in Coast of Cox’s Bazar, Bangladesh\u003c/p\u003e","fulltext":[{"header":"Introduction","content":" \u003cp\u003eSeaweed is a colloquial term for the common name of mostly macroscopic and multicellular marine algae, which do not have root systems or flowers, leaves, stems, fruits and seeds and generally grow and live attached to rock or other hard substrata below the high-water mark or remain drifted in the oceans[\u003cspan additionalcitationids=\"CR2 CR3\" citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e]. Seaweeds is considered as a high profile commercial marine biota for its variety of uses, like raw materials of bio-chemicals (agar, agarose, algin, and carrageenan), dyes, food, feed, enzymes and drugs [\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e]. Bangladesh is thought to be in the transitional zone for the flora and fauna of the Indian subcontinent and Southeast Asia and is part of the Indo-Burma biodiversity hotspot [\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e]. It has 710-km-long coastline and a 25,000-sq-km coastal area which is supported by a variety of land use practices. The seacoast of Bangladesh is considered as one of the unreached areas of the world in the field of phycology. This coastal area, with both sandy and muddy beaches, estuaries and mangrove swamps, provides substrates and habitats for the cultivation of various kinds of seaweeds, according to experts. \u003cem\u003eGracilaria\u003c/em\u003e species are notable for their economic importance as various products like agar, human food, animal feed, fertilizer, drugs, and biofuel are produced from it [\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e]. With a cosmopolitan distribution and high growth rates, this species is considered as good candidates for cultivation in various parts of the world [\u003cspan citationid=\"CR8\" class=\"CitationRef\"\u003e8\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eSeaweed culture in Bangladesh is still in an initial stage. However, a few researchers are involved in seaweed cultivation in the south-eastern and south-western coasts of Bangladesh and the methods of cultivation of seaweeds using indigenous materials like bamboo and rope [\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e]. There are a few number of experiment conducted on culture of seaweed in Bangladesh where all of them either focused on the adaptation of culture technique based on environmental parameters or identifying potential sites for seaweed [\u003cspan additionalcitationids=\"CR10\" citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e]. No experiment conducted regarding other possible factors which has influence on the yield performance of the seaweed culture in Bangladesh. Harrison and Hurd [\u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e12\u003c/span\u003e] reported that success in cultivating seaweed depends on both of the knowledge of ecophysiological characteristics of the species and on the important factors for seaweed growth that are responsible for better yields performance.\u003c/p\u003e \u003cp\u003eHence, the aim of this study was to observe the effect of some crucial factors such as lunar cycle, harvesting interval, planting system and seeding distance on the production of seaweed \u003cem\u003eGracilaria tenuistipitata\u003c/em\u003e in Nuniarchara coast, Cox\u0026rsquo;s Bazar.\u003c/p\u003e "},{"header":"Results","content":"\u003cp\u003e\u003cstrong\u003eWater Quality Parameters\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eSeaweed culture needs suitable physico-chemical parameters. The mean (\u0026plusmn;SD) values of different water quality parameters during the experimental period are presented in Table 1.\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003eTable 1.\u003c/span\u003e\u003c/strong\u003e\u003cspan style='font-size:16px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e\u0026nbsp;\u003c/span\u003e\u003cspan style='font-size:16px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003eMean values of water quality parameters during experimental period of 60 days\u003c/span\u003e\u003c/p\u003e\n\u003ctable style=\"border-collapse:collapse;border:none;\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd colspan=\"6\" style=\"width: 441.5pt;border-top: 1pt solid windowtext;border-left: none;border-bottom: 1pt solid windowtext;border-right: none;padding: 0in;height: 32.7pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:8.85pt;margin-right:86.85pt;margin-bottom:.0001pt;margin-left:-.5in;text-align:center;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;line-height: 200%;\"\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;line-height:200%;\"\u003e\u0026nbsp;Average water quality parameters\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 77.95pt;border: none;padding: 0in;height: 42.5pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:7.55pt;margin-right:0in;margin-bottom:.0001pt;margin-left:5.35pt;text-align:center;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;line-height:200%;\"\u003eParameters\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 87.4pt;border: none;padding: 0in;height: 42.5pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:7.55pt;margin-right: 0in;margin-bottom:.0001pt;margin-left:15.9pt;text-align: left;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cspan style=\"font-size:16px;line-height:200%;\"\u003eSalinity (ppt)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 59.8pt;border: none;padding: 0in;height: 42.5pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:7.55pt;margin-right:7.1pt;margin-bottom:.0001pt;margin-left:0in;text-align:center;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cspan style=\"font-size:16px;line-height: 200%;\"\u003eTemp. (\u003csup\u003eo\u003c/sup\u003eC)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 1.15in;border: none;padding: 0in;height: 42.5pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:7.55pt;margin-right:7.05pt;margin-bottom:.0001pt;margin-left:6.95pt;text-align:center;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cspan style=\"font-size:16px;line-height: 200%;\"\u003eTransparency (cm)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 55.2pt;border: none;padding: 0in;height: 42.5pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:7.55pt;margin-right: 7.05pt;margin-bottom:.0001pt;margin-left:6.8pt;text-align: left;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cspan style=\"font-size:16px;line-height:200%;\"\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; pH \u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 78.35pt;border: none;padding: 0in;height: 42.5pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:7.55pt;margin-right:7.05pt;margin-bottom:.0001pt;margin-left:6.8pt;text-align:center;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cspan style=\"font-size:16px;line-height: 200%;\"\u003eDO (mg/L)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 77.95pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in;height: 28.65pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;text-align:center;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cspan style=\"font-size:16px;line-height: 200%;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 87.4pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in;height: 28.65pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:.05in;margin-right:11.75pt;margin-bottom:.0001pt;margin-left:0in;text-align:center;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cspan style=\"font-size:16px;line-height: 200%;\"\u003e32\u0026plusmn;0.3\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 59.8pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in;height: 28.65pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:.05in;margin-right:7.1pt;margin-bottom:.0001pt;margin-left:7.0pt;text-align:center;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cspan style=\"font-size:16px;line-height: 200%;\"\u003e22\u0026plusmn;0.1\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 1.15in;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in;height: 28.65pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:.05in;margin-right:7.05pt;margin-bottom:.0001pt;margin-left:7.05pt;text-align:center;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cspan style=\"font-size:16px;line-height: 200%;\"\u003e74.5\u0026plusmn;1.4\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 55.2pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in;height: 28.65pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:.05in;margin-right:7.05pt;margin-bottom:.0001pt;margin-left:6.85pt;text-align:center;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cspan style=\"font-size:16px;line-height: 200%;\"\u003e8.0\u0026plusmn;0.1\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 78.35pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in;height: 28.65pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:.05in;margin-right:7.05pt;margin-bottom:.0001pt;margin-left:6.85pt;text-align:center;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cspan style=\"font-size:16px;line-height: 200%;\"\u003e7.2\u0026plusmn;0.1\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003cstrong\u003eNutrient parameters\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNutrient parameters of water and soil in the seaweed beds of Nuniachara coast of Cox\u0026rsquo;s Bazar are presented in the Table 2.\u0026nbsp;\u003c/p\u003e\n\u003ch1 style='margin-top:4.55pt;margin-right:32.0pt;margin-bottom:.0001pt;margin-left:0in;text-align:justify;font-size:13px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cspan style=\"font-size:16px;line-height:200%;\"\u003eTable 2.\u0026nbsp;\u003c/span\u003e\u003cspan style=\"font-size:16px;line-height:200%;font-weight:normal;\"\u003eWater and soil nutrients (mean\u0026plusmn;Sd) of seaweed bed in Nuniachara coast of \u0026nbsp;\u003cbr\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Cox\u0026rsquo;s Bazar\u003c/span\u003e\u003c/h1\u003e\n\u003ctable style=\"margin-left:22.25pt;border-collapse:collapse;border:none;\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:2.75in;border:none;border-top:solid windowtext 1.0pt;padding:0in 0in 0in 0in;height:9.85pt;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;text-align:center;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;line-height:200%;\"\u003eWater Parameters\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:229.5pt;border:none;border-top:solid windowtext 1.0pt;padding:0in 0in 0in 0in;height:9.85pt;\"\u003e\n \u003cp style='margin-top:0in;margin-right:5.35pt;margin-bottom:.0001pt;margin-left:0in;text-align:center;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;line-height:200%;\"\u003eUnit\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 2.75in;border: none;padding: 0in;height: 19.15pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:3.5pt;margin-right:0in;margin-bottom:.0001pt;margin-left:5.75pt;text-align:center;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cspan style=\"font-size:16px;line-height:200%;\"\u003eNitrate (NO\u003csub\u003e3\u003c/sub\u003e) (mg/L)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 229.5pt;border: none;padding: 0in;height: 19.15pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:3.5pt;margin-right:5.35pt;margin-bottom:.0001pt;margin-left:9.65pt;text-align:center;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cspan style=\"font-size:16px;line-height:200%;\"\u003e0.632\u0026plusmn;0.2\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 2.75in;border: none;padding: 0in;height: 18.5pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:2.9pt;margin-right:0in;margin-bottom:.0001pt;margin-left:5.75pt;text-align:center;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cspan style=\"font-size:16px;line-height:200%;\"\u003eNitrite (NO\u003csub\u003e2\u003c/sub\u003e) (mg/L)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 229.5pt;border: none;padding: 0in;height: 18.5pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:2.9pt;margin-right:5.35pt;margin-bottom:.0001pt;margin-left:9.5pt;text-align:center;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cspan style=\"font-size:16px;line-height:200%;\"\u003e0.443\u0026plusmn;0.11\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 2.75in;border: none;padding: 0in;height: 18.1pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:2.85pt;margin-right:0in;margin-bottom:.0001pt;margin-left:5.75pt;text-align:center;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cspan style=\"font-size:16px;line-height:200%;\"\u003eSulphate (S) (mg/L)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 229.5pt;border: none;padding: 0in;height: 18.1pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:2.85pt;margin-right:5.35pt;margin-bottom:.0001pt;margin-left:9.65pt;text-align:center;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cspan style=\"font-size:16px;line-height:200%;\"\u003e10,234.25\u0026plusmn;125.3\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 2.75in;border: none;padding: 0in;height: 18.3pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:3.3pt;margin-right:0in;margin-bottom:.0001pt;margin-left:5.75pt;text-align:center;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cspan style=\"font-size:16px;line-height:200%;\"\u003eCalcium (Ca) (ppm)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 229.5pt;border: none;padding: 0in;height: 18.3pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:3.3pt;margin-right:5.35pt;margin-bottom:.0001pt;margin-left:9.65pt;text-align:center;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cspan style=\"font-size:16px;line-height:200%;\"\u003e437.30\u0026plusmn;7.1\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 2.75in;border: none;padding: 0in;height: 18.55pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:.05in;margin-right:0in;margin-bottom:.0001pt;margin-left:5.75pt;text-align:center;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;line-height: 200%;\"\u003eSoil parameters\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 229.5pt;border: none;padding: 0in;height: 18.55pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;text-align:left;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cspan style=\"font-size:16px;line-height:200%;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 2.75in;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in;height: 18.55pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:.05in;margin-right:0in;margin-bottom:.0001pt;margin-left:5.75pt;text-align:center;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cspan style=\"font-size:16px;line-height:200%;\"\u003eSoil calcium (Ca) (ppm)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 229.5pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in;height: 18.55pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:.05in;margin-right:5.35pt;margin-bottom:.0001pt;margin-left:9.65pt;text-align:center;font-size:15px;font-family:\"Times New Roman\",serif;line-height:200%;'\u003e\u003cspan style=\"font-size:16px;line-height:200%;\"\u003e16,387.32\u0026plusmn;103.5\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003cstrong\u003eEffect of lunar cycle on the yields of\u003c/strong\u003e \u003cstrong\u003e\u003cem\u003eGracilaria tenuistipitata\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eIn this experiment in T\u003csub\u003e0,\u003c/sub\u003e seeds of seaweeds were transplanted three days after full moon and harvested three days before next full moon. On the other hand in T\u003csub\u003e1,\u0026nbsp;\u003c/sub\u003eseaweeds were harvested and transplanted in between two consecutive full moons. It is found that, full moon has impact on the yield performance of \u003cem\u003eGracilaria tenuistipitata\u0026nbsp;\u003c/em\u003e(Table 3)\u003cstrong\u003e\u003cem\u003e.\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp style='margin-top:12.0pt;margin-right:0in;margin-bottom:8.0pt;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003eTable 3.\u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003cspan style='font-size:16px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003eEffect of lunar cycle on the yield performance of\u003c/span\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003cem\u003e\u003cspan style='font-size:16px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003eGracilaria tenuistipitata\u003c/span\u003e\u003c/em\u003e\u003cspan style='font-size:16px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cdiv align=\"center\" style='margin-top:0in;margin-right:0in;margin-bottom:8.0pt;margin-left:0in;line-height:107%;font-size:15px;font-family:\"Calibri\",sans-serif;'\u003e\n \u003ctable style=\"width:95.44%;border-collapse:collapse;border:none;\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 16.04%;border: 1pt solid black;padding: 0in 5.4pt;height: 18.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;text-indent:-.9pt;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003eTreatments\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 27.92%;border-top: 1pt solid black;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid windowtext;padding: 0in 5.4pt;height: 18.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003eFull moon during experiment\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 26.1417%; border-top: 1pt solid black; border-right: 1pt solid black; border-bottom: 1pt solid black; border-image: initial; border-left: none; padding: 0in 5.4pt; height: 18.4pt; vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003eFresh yield (t ha\u003csup\u003e-1\u003c/sup\u003e)\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 29.9213%; border-top: 1pt solid black; border-right: 1pt solid black; border-bottom: 1pt solid black; border-image: initial; border-left: none; padding: 0in 5.4pt; height: 18.4pt; vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;text-indent:-.9pt;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003eDry yield (t ha\u003csup\u003e-1\u003c/sup\u003e)\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 16.04%;border-right: 1pt solid black;border-bottom: 1pt solid black;border-left: 1pt solid black;border-image: initial;border-top: none;padding: 0in 5.4pt;height: 27.1pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;text-indent:-.9pt;'\u003e\u003cspan style='font-size:16px;line-height: 200%;font-family:\"Times New Roman\",serif;'\u003eT\u003csub\u003e0\u003c/sub\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd rowspan=\"4\" style=\"width:27.92%;border-top:none;border-left: none;border-bottom:solid black 1.0pt;border-right:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;height:27.1pt;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:200%;font-family: \"Times New Roman\",serif;'\u003e12 December\u003c/span\u003e\u003c/p\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:200%;font-family: \"Times New Roman\",serif;'\u003eand\u003c/span\u003e\u003c/p\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:200%;font-family: \"Times New Roman\",serif;'\u003e11 January\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 26.1417%; border-top: none; border-left: none; border-bottom: 1pt solid black; border-right: 1pt solid black; padding: 0in 5.4pt; height: 27.1pt; vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:200%;font-family: \"Times New Roman\",serif;'\u003e9.12 \u0026plusmn; 0.66\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 29.9213%; border-top: none; border-left: none; border-bottom: 1pt solid black; border-right: 1pt solid black; padding: 0in 5.4pt; height: 27.1pt; vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;text-indent:-.9pt;'\u003e\u003cspan style='font-size:16px;line-height: 200%;font-family:\"Times New Roman\",serif;'\u003e1.55 \u0026plusmn;0.19\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 16.04%;border-right: 1pt solid black;border-bottom: 1pt solid black;border-left: 1pt solid black;border-image: initial;border-top: none;padding: 0in 5.4pt;height: 26.35pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;text-indent:-.9pt;'\u003e\u003cspan style='font-size:16px;line-height: 200%;font-family:\"Times New Roman\",serif;'\u003eT\u003csub\u003e1\u003c/sub\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 26.1417%; border-top: none; border-left: none; border-bottom: 1pt solid black; border-right: 1pt solid black; padding: 0in 5.4pt; height: 26.35pt; vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:200%;font-family: \"Times New Roman\",serif;'\u003e7.97\u0026plusmn; 0.80\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 29.9213%; border-top: none; border-left: none; border-bottom: 1pt solid black; border-right: 1pt solid black; padding: 0in 5.4pt; height: 26.35pt; vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;text-indent:-.9pt;'\u003e\u003cspan style='font-size:16px;line-height: 200%;font-family:\"Times New Roman\",serif;'\u003e1.35\u0026plusmn;0.14\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 16.04%;border-right: 1pt solid black;border-bottom: 1pt solid black;border-left: 1pt solid black;border-image: initial;border-top: none;padding: 0in 5.4pt;height: 17.95pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;text-indent:-.9pt;'\u003e\u003cspan style='font-size:16px;line-height: 200%;font-family:\"Times New Roman\",serif;color:black;background:white;'\u003e∆T=\u003c/span\u003e\u003cspan style='font-size:16px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e\u0026nbsp;T\u003csub\u003e0-\u003c/sub\u003eT\u003csub\u003e1\u003c/sub\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 26.1417%; border-top: none; border-left: none; border-bottom: 1pt solid black; border-right: 1pt solid black; padding: 0in 5.4pt; height: 17.95pt; vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:200%;font-family: \"Times New Roman\",serif;'\u003e1.15\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 29.9213%; border-top: none; border-left: none; border-bottom: 1pt solid black; border-right: 1pt solid black; padding: 0in 5.4pt; height: 17.95pt; vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;text-indent:-.9pt;'\u003e\u003cspan style='font-size:16px;line-height: 200%;font-family:\"Times New Roman\",serif;'\u003e0.20\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 16.04%;border-right: 1pt solid black;border-bottom: 1pt solid black;border-left: 1pt solid black;border-image: initial;border-top: none;padding: 0in 5.4pt;height: 20.15pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;text-indent:-.9pt;'\u003e\u003cspan style='font-size:16px;line-height: 200%;font-family:\"Times New Roman\",serif;color:black;background:white;'\u003e% increase\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 26.1417%; border-top: none; border-left: none; border-bottom: 1pt solid black; border-right: 1pt solid black; padding: 0in 5.4pt; height: 20.15pt; vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:200%;font-family: \"Times New Roman\",serif;'\u003e14.43\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 29.9213%; border-top: none; border-left: none; border-bottom: 1pt solid black; border-right: 1pt solid black; padding: 0in 5.4pt; height: 20.15pt; vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;text-indent:-.9pt;'\u003e\u003cspan style='font-size:16px;line-height: 200%;font-family:\"Times New Roman\",serif;'\u003e14.81\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003c/table\u003e\n\u003c/div\u003e\n\u003cp\u003eThe yields found higher in T\u003csub\u003e0\u003c/sub\u003e (where seeds were transplanted after full moon and harvested before the next full moon) than T\u003csub\u003e1\u0026nbsp;\u003c/sub\u003e(in between two consecutive full moons).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eEffect of harvesting interval on the yields of \u003cem\u003eGracilaria tenuistipitata\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eIn this case, the impact of harvesting interval (10, 20, 30, 40 days) on the yield performance of Gracillaria\u003cem\u003e\u0026nbsp;tenuistipitata\u0026nbsp;\u003c/em\u003epresented in figure 1.\u003c/p\u003e\n\u003cp\u003eIt is found that the production increased in T\u003csub\u003e1\u003c/sub\u003e, T\u003csub\u003e2\u003c/sub\u003e and in T\u003csub\u003e3\u003c/sub\u003e where the yields were highest in T\u003csub\u003e3\u0026nbsp;\u003c/sub\u003eand the production decreased in T\u003csub\u003e4.\u0026nbsp;\u003c/sub\u003eThese results indicate that 30 days of interval are the peak period to harvest the seaweed.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eEffect of planting system on the yield\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eTwo methods of planting system were followed to see the yield performance of\u003cem\u003e\u0026nbsp;Gracilaria\u003c/em\u003e species. It is found that the yield performance is better in semi floating single line (T\u003csub\u003e1\u003c/sub\u003e) than semi floating double line (T\u003csub\u003e2\u003c/sub\u003e) (Table 4 and 5).\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:8.0pt;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003eTable 4 A. Yield \u0026nbsp; Performance of\u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003cstrong\u003e\u003cem\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003eGracilaria\u003c/span\u003e\u003c/em\u003e\u003c/strong\u003e\u003cstrong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003cem\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003et\u003cstrong\u003eenuistipitata\u0026nbsp;\u003c/strong\u003e\u003c/span\u003e\u003c/em\u003e\u003cstrong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003ein semi floating single line (T\u003csub\u003e1\u003c/sub\u003e)\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003ctable style=\"border-collapse:collapse;border:none;\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 150.25pt;border-top: 1pt solid windowtext;border-left: none;border-bottom: 1pt solid windowtext;border-right: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003eNo. of row\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.25pt;border-top: 1pt solid windowtext;border-left: none;border-bottom: 1pt solid windowtext;border-right: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003eFresh weight (ton/ha)\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.3pt;border-top: 1pt solid windowtext;border-left: none;border-bottom: 1pt solid windowtext;border-right: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003eDry weight (ton/ha)\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 150.25pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e1\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.25pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e12.78\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.3pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e2.03\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 150.25pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e2\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.25pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e13.63\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.3pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e2.18\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 150.25pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e3\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.25pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e11.99\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.3pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e1.92\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 150.25pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e4\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.25pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e13.52\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.3pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e2.16\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 150.25pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e5\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.25pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e11.79\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.3pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e1.87\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 150.25pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003eMean\u003c/span\u003e\u003c/strong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e\u0026plusmn;\u003cstrong\u003eSD\u003c/strong\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.25pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e12.74\u003c/span\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e\u0026plusmn;0.85\u003csup\u003eA\u003c/sup\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.3pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e2.03\u003c/span\u003e\u003cspan style='font-size:13px;line-height:200%;font-family: \"Times New Roman\",serif;'\u003e\u0026plusmn;0.14\u003csup\u003eA\u003c/sup\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:8.0pt;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:8.0pt;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003eTable 4B. \u0026nbsp; Yield Performance of\u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003cstrong\u003e\u003cem\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003eGracilaria\u0026nbsp;\u003c/span\u003e\u003c/em\u003e\u003c/strong\u003e\u003cem\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003et\u003cstrong\u003eenuistipitata\u003c/strong\u003e\u003c/span\u003e\u003c/em\u003e\u003cstrong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e\u0026nbsp;species in semi floating double line (T\u003csub\u003e2\u003c/sub\u003e)\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003ctable style=\"border-collapse:collapse;border:none;\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 150.25pt;border-top: 1pt solid windowtext;border-left: none;border-bottom: 1pt solid windowtext;border-right: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003eNo. of row\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.25pt;border-top: 1pt solid windowtext;border-left: none;border-bottom: 1pt solid windowtext;border-right: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003eFresh weight (ton/ha)\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.3pt;border-top: 1pt solid windowtext;border-left: none;border-bottom: 1pt solid windowtext;border-right: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003eDry weight (ton/ha)\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 150.25pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e1\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.25pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e7.49\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.3pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e1.29\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 150.25pt;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e2\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.25pt;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e6.92\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.3pt;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e1.15\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 150.25pt;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e3\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.25pt;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e7.91\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.3pt;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e1.19\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 150.25pt;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e4\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.25pt;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e6.75\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.3pt;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e1.09\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 150.25pt;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e5\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.25pt;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e7.31\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.3pt;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e1.24\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 150.25pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003eMean\u003c/span\u003e\u003c/strong\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e\u0026plusmn;\u003cstrong\u003eSD\u003c/strong\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.25pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e7.28\u0026plusmn;0.46\u003csup\u003eB\u003c/sup\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 150.3pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:13px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e1.19\u003c/span\u003e\u003cspan style='font-size:13px;line-height:200%;font-family: \"Times New Roman\",serif;'\u003e\u0026plusmn;0.08\u003csup\u003eB\u003c/sup\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003ctable style=\"border-collapse:collapse;border:none;\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:8.0pt;margin-left:0in;line-height:200%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003eTable 5. Yield Performance of\u0026nbsp;\u003c/span\u003e\u003cem\u003e\u003cspan style='font-size:16px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003eGracilaria\u0026nbsp;\u003c/span\u003e\u003c/em\u003e\u003cem\u003e\u003cspan style='font-size:16px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003etenuistipitata\u003c/span\u003e\u003c/em\u003e\u003cspan style='font-size:16px;line-height:200%;font-family:\"Times New Roman\",serif;'\u003e\u0026nbsp;species in semi floating double line \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003cbr\u003e (T\u003csub\u003e2\u003c/sub\u003e)\u003c/span\u003e\u003c/p\u003e\n\n\u003cp\u003eThe fresh yield of in semi floating single line was 12.74\u0026plusmn;0.8\u0026nbsp;t ha\u003csup\u003e-1\u003c/sup\u003e that was significantly higher than the yield in semi floating double line system (7.28\u0026plusmn;0.46 t ha\u003csup\u003e-1\u003c/sup\u003e).\u003c/p\u003e\n\u003cp\u003eThe 20 cm length of planting distance showed significantly higher amount of yields (2.30 t ha\u003csup\u003e-1\u003c/sup\u003e) than the other three treatments. T\u003csub\u003e4\u0026nbsp;\u003c/sub\u003ewhich denotes the planting distance 40 cm showed the lowest yield performance (1.5 t ha\u003csup\u003e-1\u003c/sup\u003e).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eEffect of seeding distance on the yields of\u0026nbsp;\u003c/strong\u003e\u003cstrong\u003e\u003cem\u003eGracilaria tenuistipitata\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe yield of \u003cem\u003eGracilaria tenuistipitata\u003c/em\u003e seedlings in each treatments during the cultivation periods (30 days) is graphed in figure 2. \u0026nbsp;It shows that the planting distances influence the yields of\u003cem\u003e\u0026nbsp;Gracilaria tenuistipitata\u003c/em\u003e (p\u0026lt;0.05).\u003c/p\u003e"},{"header":"Discussion","content":" \u003cp\u003eSeaweeds are not only source of food, feed and medicine but also a source of bioactive compounds which has nutritional and biomedical applications [\u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e13\u003c/span\u003e]. In recent years, seaweed cultivation has been an issue of much importance all over the world. \u003cem\u003eGracilaria tenuistipitata\u003c/em\u003e is an important seaweed species which has tolerant to a wide range of environments and an economically important raw material for agar production [\u003cspan citationid=\"CR14\" class=\"CitationRef\"\u003e14\u003c/span\u003e]. Most of the researches on seaweed farming is limited to searching the suitable location, selection of farming method and suitability of seaweed seed [\u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e, \u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e, \u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e]. The success of seaweed farming does not only depend on ecological suitability of the site, but also to a large extent on some factors that also have impact on the production performance of seaweed. In this study, we evaluated influences of some other factors like effect of lunar cycle, rope type, harvesting interval and seeding gap on the improvement of production performances.\u003c/p\u003e \u003cp\u003eIn these experiments, the water quality parameters and nutrient parameters of soil and water can be said suitable for seaweed culture according to other research findings [\u003cspan additionalcitationids=\"CR17\" citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR18\" class=\"CitationRef\"\u003e18\u003c/span\u003e]. \u003cem\u003eGracilaria\u003c/em\u003e has a high tolerance of environmental parameters [\u003cspan additionalcitationids=\"CR20\" citationid=\"CR19\" class=\"CitationRef\"\u003e19\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e21\u003c/span\u003e]. The optimum environment could improve nutrient absorption process so that it could improve the growth rate of seaweeds.[\u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e, \u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e, \u003cspan citationid=\"CR23\" class=\"CitationRef\"\u003e23\u003c/span\u003e]\u003c/p\u003e \u003cp\u003eRegarding the effect of lunar cycle, seeding and harvesting was carried considering lunar cycle as tidal effect showed strong influence on the productivity of seaweed farming. Results showed that full moon has clear influential effect on the yield of \u003cem\u003eGracilaria tenuistipitata\u003c/em\u003e since during the day of full moon and three days before and after the full moon, the wave pressure remains higher than usual. Mulford [\u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e24\u003c/span\u003e] reported that when the moon is full, this causes a stronger pull-on ocean as the Earth and Moon are aligned, which causes a more pronounced tide. In present study, reduced yield performance recorded when seaweeds were harvested and transplanted in between two consecutive full moons. On the other hand, better yield performance recorded when the seeds were transplanted three days after full moon and harvested three days before next full moon. This was happened because during full moon in the middle or late of cultivation cycle, highest high tide and waves occur. Stronger high tide reduced the yield by storming off the long crop rope of \u003cem\u003eGracilaria tenuistipitata.\u003c/em\u003e Yield may be increased by 10 to 15 percent if we seed the crop three days before the full moon and harvest it three days after the next full moon (24 days field duration), instead of seeding at first day of the month and harvesting at last day (30 days field duration). Thus, we can save 6 days which can be used to dry and processing the harvest and also to take preparations for the next seeding. It has been found that harvesting duration also another factor which may influence on the productivity of seaweed farming. Timely harvesting of any crops ensures good crop quality and high market value. Another experiment also carried out to search out the suitable harvesting interval for obtaining better yield. In our present study, it is found that harvesting seaweeds after 30 days of seeding showed maximum yield performance. Reduced yield performance found when seaweeds were harvested after 40 days of seeding. The reason behind this could be rotting of the bunch of ropes of seaweed after 30 days of period and loss of the yield of seaweed has been occurred. Although no research findings is available in the literature regarding this factor, our study revealed that 30 days of harvesting interval is the peak period to harvest. Harvesting needs to be carried out in such a way as to maintain \u003cem\u003epeak\u003c/em\u003e productivity.\u003c/p\u003e \u003cp\u003eRope type has significant impact on the yield performance of the \u003cem\u003eGracilaria tenuistipitata.\u003c/em\u003e In our study, it has been seen that semi floating single line rope method was found better over semi-floating double line rope method and the yield was significantly higher in semi floating single line rope method. The higher yield was found due to fact that seeds of double line floating system were more submerged than the single line floating system and it happened to decompose the seeds of seaweeds and reduced the yield. Additionally, penetration of sunlight was less due to double line floating system which ultimately hampers the photosynthesis. Most of the researchers mainly focused on methodological approaches and main cultivation methods and discusses different problems arising during the application of these methods [\u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e25\u003c/span\u003e, \u003cspan citationid=\"CR26\" class=\"CitationRef\"\u003e26\u003c/span\u003e] but no experiment found to be conducted regarding the planting method on the yield performance of \u003cem\u003eGracilaria tenuistipitata.\u003c/em\u003e\u003c/p\u003e \u003cp\u003eSeeding gap is a crucial factor for seaweed production. Neish [\u003cspan citationid=\"CR27\" class=\"CitationRef\"\u003e27\u003c/span\u003e] reported that the suitable planting distance will provide wider water circulation which take nutrients leading to enhance diffusion process in upgrading the metabolic and growth rate. In our present study, 20 cm length of seeding gap resulted significantly higher amount of yields. In this study, the 20cm-seeding gap is assumed to be suitable for receiving the intensity of sunlight in all thalli parts of \u003cem\u003eGracilaria\u003c/em\u003e, while in the other planting distance (10,30 \u0026amp; 40 cm) might be too narrow or too wide that indicates thalli\u0026rsquo;s surface cover each other and also covered by filamentous epiphyte algae that impede sunlight absorption. These findings are in line with a report of Reddy \u003cem\u003eet al.\u003c/em\u003e [\u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e28\u003c/span\u003e] and Aslin \u003cem\u003eet al.\u003c/em\u003e [\u003cspan citationid=\"CR29\" class=\"CitationRef\"\u003e29\u003c/span\u003e] who found similar result in their experiment.\u003c/p\u003e \u003cp\u003eTaking all those factors into account, these studies revealed that \u003cem\u003eGracilaria tenuistipitata\u003c/em\u003e species could be cultured in our coast particularly in Nuniachara coast of Cox\u0026rsquo;s Bazar. However, for getting maximum production, some effects such as lunar cycle, rope type, harvesting intervals of the seaweed and seeding gap need to be considered as our studies finds that there are influential impact of this factors on the yield performance of \u003cem\u003eGracilaria\u003c/em\u003e species. So, further research is needed to find out the effect of various factors on the yield performance of seaweed.\u003c/p\u003e "},{"header":"Methods","content":"\u003cp\u003e\u003cstrong\u003eStudy area:\u003c/strong\u003e Study was conducted from December 2019 to January 2020 in Nuniachara of Cox\u0026rsquo;s Bazar sadar. \u0026nbsp;The cultivation of seaweeds in open sea is being carried out using one-step seed production method which requires healthy seaweeds. Taking the above considerations into account, experiment site has been primarily selected at Nuniachara coast of Cox\u0026rsquo;s Bazar sadar.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCollection of seaweed seed: \u003c/strong\u003eWild \u003cem\u003eG. tenuistipitata \u003c/em\u003eas seed was collected from Saint Martin\u0026rsquo;s Island (92\u0026ordm;28ʹ40.12ʺE and 20\u0026ordm;65ʹ51.43ʺN) of Bay of Bengal of Bangladesh. Seed collection from seabed was permitted from the local government and authenticated the botanical identification of seaweed species as the voucher specimen has been previously deposited at BFRI herbarium.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u0026nbsp;Production of seeds:\u003c/strong\u003e The farming/cultivation of seaweeds in open sea was carried out using one-step seed production method. Seeds for seaweed farming was produced by one-step (directly using cut pieces and tied with the ropes) and multi-step (inducing spore release by a particular seaweed in the green house and allowing spores to grow on ropes and nets) methods.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eSeaweed culture method: \u003c/strong\u003eThe seeded ropes were arranged in single or double lines in the open sea.\u0026nbsp; Each line was kept attached with two bamboo poles so that it could not be washed away. Plastic floats @ 5 pieces per 25 m long rope were used to keep the ropes moving up and downwards during tides. No fertilizer, insecticides and pesticides were used during seaweed cultivation.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eWater parameters:\u003c/strong\u003e Water temperature, salinity, transparency, pH and dissolved oxygen (DO) were checked every 15 days at the cultivation sites. Transparency was measured using Secci disc. A digital DO meter (HACH, USA) was used to determine the dissolved oxygen content of water. pH was measured using a digital pH meter (HACH, USA). Salinity was determined using refractometer.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u0026nbsp;Collection and analysis of soil: \u003c/strong\u003eSoil samples from the sites were collected and after collection, samples were brought back to the laboratory within 6 h for analysis. In the laboratory, the samples were dried and powdered, sieved and kept in a desiccator until further analysis.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eOptimization of different factors\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eExperiment 1: Effect of lunar cycle\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThis study was conducted to find out the effect of lunar cycle on the yield performance of \u003cem\u003eGracilaria \u003c/em\u003e\u003cem\u003etenuistipitata. \u003c/em\u003eIn this experiment, T\u003csub\u003e0\u0026nbsp; \u003c/sub\u003eindicates control where the seeds were transplanted three days after full moon and harvested three days before next full moon. On the other hand, T\u003csub\u003e1\u0026nbsp; \u003c/sub\u003e\u0026nbsp;indicates that seaweeds were harvested and transplanted in between two consecutive full moons where one highest high tide occurred.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eExperiment 2: Effect of harvesting interval\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eIn this experiment, different harvesting intervals (T\u003csub\u003e1 = \u003c/sub\u003e10 days, T\u003csub\u003e2 = \u003c/sub\u003e20 days, T\u003csub\u003e3 =\u003c/sub\u003e 30 days, T\u003csub\u003e4 =\u003c/sub\u003e40 days) were taken to determine the best harvesting time with \u003cem\u003eGracilaria \u003c/em\u003e\u003cem\u003etenuistipitata \u003c/em\u003eby harvesting after 10 days, 20 days, 30 days and 40 days of seeding, respectively for getting maximum production.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003e\u0026nbsp;\u003c/em\u003e\u003c/strong\u003e\u003cstrong\u003e\u003cem\u003eExperiment 3: Effect of rope type\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eTwo types of rope (where T\u003csub\u003e1 \u003c/sub\u003edenotes semi-floating single line method and T\u003csub\u003e2 \u003c/sub\u003e\u0026nbsp;denotes semi-floating double line method) were followed to evaluate the effect of this method on the yield performance of \u003cem\u003eGracilaria \u003c/em\u003e\u003cem\u003etenuistipitata.\u003c/em\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eExperiment 4:Effect of seeding gap\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThis experiment was carried out for determining the effect of seeding gap where T\u003csub\u003e1\u003c/sub\u003e,T\u003csub\u003e2\u003c/sub\u003e,T\u003csub\u003e3\u003c/sub\u003e and T\u003csub\u003e4\u003c/sub\u003e indicates 10 cm, 20 cm, 30 cm and 40 cm seeding gap, respectively on the yield performance of \u003cem\u003eGracilaria \u003c/em\u003e\u003cem\u003etenuistipitata.\u003c/em\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u0026nbsp;Statistical analysis:\u003c/strong\u003e Data were statistically analyzed by statistical package SPSS version 16.0 (SPSS Inc., Chicago, IL, USA). Before all analysis data were analyzed for normality by probability plots and for homogeneity of variances by Levene's test. One way ANOVA was used to determine the significance of each parameter among different treatments. If a main effect was significant, the ANOVA was followed by Tukey's test. Level of significance was made at 95% probability level.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eAcknowledgements: \u003c/strong\u003eThis research was funded by Krishi Gobeshona Foundation (KGF), Ministry of Agriculture, Bangladesh.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthor\u0026rsquo;s Contribution: \u003c/strong\u003eBokhtiar, Ali, Chowdhury, Ahmed designed and executed this research. Hassan and Ahmed reviewed this manuscript. Bhuiyan, Mashuk, and Rahman performed research design, data compilation, manuscript preparation and draft manuscript preparation. Rafiquzzaman supervised research activities.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\u003cli\u003e\u003cspan\u003eChapman, V. J. Seaweeds and Their Uses. Soil Sci.\u003cb\u003e74\u003c/b\u003e (\u003cb\u003e6\u003c/b\u003e), 299 (1973).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eOkazaki, A. Seaweeds and Their Uses in Japan; Tokai University Press: [Tokyo], (1971).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eRound, F. E. The Biology of the Algae, 2nd ed.; Hodder: London, England, (1976).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eMcHugh, D. J. A guide to the seaweed industry FAO Fisheries Technical Paper. Foodand Agriculture Organization of the United Nations, Rome.\u003cb\u003e441pp\u003c/b\u003e (2003)\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eAthithan, S. Growth performance of a seaweed, \u003cem\u003eKappaphycus alvarezii\u003c/em\u003e under lined earthen pond condition in Tharuvaikulam of Thoothukudi coast, South East of India.Res J Anim Vet Fish Sci. \u003cb\u003e2\u003c/b\u003e, 6\u0026ndash;10 (2014).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eAl Mamun Siddiqui, A.; Kashem, M. A.; Mondal, M. A. I.; Shafiuddin, M. Commercially Important Seaweed Cultivation and Its Potentials for the Coastal Areas of Cox\u0026rsquo;s Bazar, Bangladesh. Int. j. fish. aquat. stud. \u003cb\u003e7\u003c/b\u003e (\u003cb\u003e5\u003c/b\u003e), 463\u0026ndash;470 (2019).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eBuchholz, C. M.; Krause, G.; Buck, B. H. Seaweed and Man. In Ecological Studies; Springer Berlin Heidelberg: Berlin, Heidelberg, pp\u0026nbsp;471\u0026ndash;493 (2012).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003ePereira, R.; Yarish, C. Mass Production of Marine Macroalgae. In Encyclopedia of Ecology; Elsevier,pp\u0026nbsp;2236\u0026ndash;2247 (2008).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eIslam, M. M.; Khan, M. S. K.; Hasan, J.; Mallick, D.; Hoq, M. E. Seaweed Hypnea Sp. Culture in Cox\u0026rsquo;s Bazar Coast, Bangladesh. Bangladesh J. Zool. \u003cb\u003e45\u003c/b\u003e (\u003cb\u003e1\u003c/b\u003e), 37\u0026ndash;46 (2017).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eHoq, M. E.; Haque, M. A.; Islam, M. M. Feasibility of Seaweed Culture in Inani and Bakkhali Coast of Cox\u0026rsquo;s Bazar, Bangladesh. Pak. J. Mar. Sci.\u003cb\u003e25\u003c/b\u003e (\u003cb\u003e1\u003c/b\u003e \u0026amp; \u003cb\u003e2\u003c/b\u003e), 27\u0026ndash;36 (2016).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eAbdullah Al, M.; Akhtar, A.; Rahman, M. F.; Kamal, A. H. M.; Karim, N. U.; Hassan, M. L. Habitat Structure and Diversity Patterns of Seaweeds in the Coastal Waters of Saint Martin\u0026rsquo;s Island, Bay of Bengal, Bangladesh. Reg. Stud. Mar. Sci. \u003cb\u003e33\u003c/b\u003e (100959), 100959 (2020).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eHarrison, P. J.; Hurd, C. L. Nutrient Physiology of Seaweeds: Application of Concepts to Aquaculture. Cah. Biol. Mar.\u003cb\u003e42\u003c/b\u003e (\u003cb\u003e1\u0026ndash;2\u003c/b\u003e), 71\u0026ndash;82 (2001).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eKumar, C. S.; Ganesan, P.; Suresh, P. V.; Bhaskar, N. Seaweeds as a Source of Nutritionally Beneficial Compounds - A Review. J. Food Sci. Technol.45 (1), 1\u0026ndash;13 (2008).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eYarnpakdee, S.; Benjakul, S.; Kingwascharapong, P. Physico-Chemical and Gel Properties of Agar from Gracilaria Tenuistipitata from the Lake of Songkhla, Thailand. Food Hydrocoll. \u003cb\u003e51\u003c/b\u003e, 217\u0026ndash;226 (2015).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eImran, M.H., Islam, M.S., Kabir, M.H., Meghla, N.T. and Islam, M.T. SURFACE WATER QUALITIES IN COASTAL MOHESHKHALI FISHING ZONES OF BANGLADESH. Bangladesh J.38,1\u0026ndash;12 (2020).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eKim, J. K.; Yarish, C.; Pereira, R. Tolerances to Hypo-Osmotic and Temperature Stresses in Native and Invasive Species Of Gracilaria(Rhodophyta). Phycologia, \u003cb\u003e55 (3\u003c/b\u003e), 257\u0026ndash;264 (2016).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eZafar, M. Seaweed culture in Bangladesh holds promise. Infofish International, 8\u0026ndash;10 (2005).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eZafar, M. Seaweed culture (\u003cem\u003eHypnea\u003c/em\u003e sp.) in Bangladesh-Seaweed Culture Manual-1,Institute of Marine Science and Fisheries, University of Chittagong, Chittagong, Bangladesh (2007).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eYokoyama, S. Molecular Bases of Color Vision in Vertebrates. Genes Genet. Syst.\u003cb\u003e74\u003c/b\u003e (\u003cb\u003e5\u003c/b\u003e), 189\u0026ndash;199 (1999).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eRaikar, S. V.; Iima, M.; Fujita, Y. Effect of Temperature, Salinity and Light Intensity on the Growth of \u003cem\u003eGracilaria spp\u003c/em\u003e (Gracilariales, Rhodophyta) from Japan, Malaysia and India. Indian J. Mar. Sci. (2001).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eAbreu, M. H.; Pereira, R.; Buschmann, A. H.; Sousa-Pinto, I.; Yarish, C. Nitrogen Uptake Responses of Gracilaria Vermiculophylla (Ohmi) Papenfuss under Combined and Single Addition of Nitrate and Ammonium. J. 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Biol.\u003cb\u003e36\u003c/b\u003e (\u003cb\u003e4\u003c/b\u003e), 227\u0026ndash;242 (2010).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eValderrama, D. Social and Economic Dimensions of Seaweed Farming: A Global Review (2012).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eNeish, I. C. The Eucheuma Seaplant Handbook; Vol I: Agronomy, Biology and Culture System. Seaplantnet Technical Monograph. 36 pp (2005).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eReddy, C. R. K.; Kumar, G. R. K.; Siddhanta, A. K.; Tewari, A.; Eswaran, K. In Vitro Somatic Embryogenesis and Regeneration of Somatic Embryos from Pigmented Callus of Kappaphycus Alvarezii (Doty) Doty (Rhodophyta, Gigartinales)1. J. Phycol.\u003cb\u003e39\u003c/b\u003e (\u003cb\u003e3\u003c/b\u003e), 610\u0026ndash;616 (2003).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eAslin, L. O., Supryiono, E., Nirmala, K., Nurjanah, D., \u0026amp; Soelistyowati, T. The effects of planting distances and seedling sources on Kappaphycus alvarezii growth.In IOP Conference Series: Earth and Environmental Science, 278 pp (2019).\u003c/span\u003e\u003c/li\u003e\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Seaweed, Gracilaria tenuistipitata, Cox’s Bazar, Bangladesh","lastPublishedDoi":"10.21203/rs.3.rs-567666/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-567666/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThis research was designed to find out the effect of different factors such as influence of lunar cycle, harvesting interval, rope type and seeding gap on the production of \u003cem\u003eG. tenuistipitata\u003c/em\u003e in coast of Cox\u0026rsquo;s Bazar. Duration of these experiments were sixty days and all the parameters were recorded fortnightly. Monitoring of water quality parameters indicated that salinity, temperature, transparency, pH and DO were suitable for seaweed cultivation. In determining lunar cycle effect, results envisaged that fresh yield was 14.43% increased when seeding and harvesting time was selected considering the moon cycle. Regarding the selection of harvesting interval, it was found that T\u003csub\u003e3\u003c/sub\u003e (30 days interval) was the best to harvest the seaweed whereas T\u003csub\u003e4\u003c/sub\u003e (40 days interval) showed decreasing trend in production. Our study also found that semi floating single line showed better yield performance compared to semi floating double line system. In case of influence on seeding gap, it has been found that 20 cm gap between two seed showed the highest yields followed by 10 cm, 30 cm and 40 cm, respectively. Overall, it can be concluded that yield of \u003cem\u003eG. tenuistipitata\u003c/em\u003e in coast of Cox\u0026rsquo;s Bazar could be improved considering those factors.\u003c/p\u003e","manuscriptTitle":"Yield Improvement of Gracilaria tentuistipitata by Optimizing Different aspects in Coast of Cox’s Bazar, Bangladesh","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2021-06-07 21:42:45","doi":"10.21203/rs.3.rs-567666/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Major revision","date":"2021-10-11T04:36:11+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2021-09-01T12:12:58+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"e566a3bc-aac3-43d1-aece-86751c684212","date":"2021-08-17T12:00:32+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"cd5409bb-90a4-4231-b084-54fabf949d63","date":"2021-08-17T10:33:32+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2021-08-08T16:07:51+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2021-08-08T11:32:01+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2021-06-02T09:26:31+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2021-06-01T04:23:53+00:00","index":"","fulltext":""},{"type":"submitted","content":"Scientific Reports","date":"2021-05-27T14:08:36+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"b0009ecd-e4d0-477b-ba81-e4b37dc815f8","owner":[],"postedDate":"June 7th, 2021","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"under-review","subjectAreas":[{"id":4861142,"name":"Plant Physiology and Morphology"},{"id":4861143,"name":"Agroecology"},{"id":4861144,"name":"Oceanography"}],"tags":[],"updatedAt":"2022-01-04T11:14:15+00:00","versionOfRecord":[],"versionCreatedAt":"2021-06-07 21:42:45","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-567666","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-567666","identity":"rs-567666","version":["v1"]},"buildId":"7rjqhiLT3MXkJMwkYKINL","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}