Expression of DNMT1 and DNMT3B in blood and peritoneal fluid of endometriosis patients

Objective To study the relationship between DNA methyltransferase(DNMT)1,DNMT 3B and the development of endometriosis(EMS)by detecting the level of DNMT1 and DNMT3Bin both blood and peritoneal fluid in EMS.Methods The levels of DNMT1 and DNMT 3Bin 56 EMS patients(35withⅠ～Ⅱstages and 21 with Ⅲ～Ⅳ stages)and 25 control patients were measured by enzyme linked immunoabsorbent assay(ELISA).Results The blood level of DNMT1,DNMT 3Band the peritoneal level of DNMT3 Bin EMS group were higher than that in control group(P 0.05).The blood levels of DNMT1 and 3Bin patients withⅢ～Ⅳstages were higher than that in patients withⅠ～Ⅱstages and controls,and DNMT3BinⅠ～Ⅱstages was also higher than controls(P0.05).The peritoneal fluid levels of DNMT1 and 3Bin patients withⅠ～Ⅱstages were higher than that in patients withⅢ～Ⅳstages and controls,and DNMT3 Blevel among the three groups had statistically significance(P0.05),while DNMT1 level in the three groups had no obvious difference(P0.05).Peritoneal fluid DNMT1 level in proliferative phrase was higher than that in secretory phase(P=0.007).DNMT1 and DNMT3Blevel had positive correlation in blood,as well as in peritoneal fluid(rserum=0.252,Pserum=0.023;rPF=0.379,PPF=0.000).DNMT3 Blevel in blood and peritoneal fluid had positive correlation,while this relationship was not found in terms of DNMT1.Conclusion The levels of DNMT1 and DNMT3Bin EMS were increased,and different stages of EMS had different variation both in blood and in local peritoneal cavity,which indicate that DNA methylation may play an important role in the genesis and development of EMS.