Antimicrobial Effect of Chitosan-Aloe vera Gel Extract Composited with Organic Oils

Antimicrobial Effect of Chitosan-Aloe vera Gel Extract Composited with Organic Oils | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Antimicrobial Effect of Chitosan-Aloe vera Gel Extract Composited with Organic Oils Ossamulu I. Famous, Peter Joseph Shaba, Evans C. Egwim, Abdulkadir Abubakar This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6177893/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 4 You are reading this latest preprint version Abstract This study evaluated the antimicrobial effects of chitosan-Aloe vera extract gel composite with organic oils (watermelon seed oil and orange seed oil) against microorganisms: Staphylococcus aureus, Pseudomonas aeruginosa, Aspergillus niger, and Aspergillus flavus. The phytochemical and antioxidant properties of the extract and the physicochemical properties of the oils were assessed. The antimicrobial activity was determined by the Minimum Fungicidal Concentration (MFC), Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC). The phytochemical analysis of Aloe vera extract revealed concentrations of alkaloids (29.72±0.65 mg/100g), tannins (142.03±0.99 mg/100g), flavonoids (113.82±1.83 mg/100g), phenols (565.68±1.36 mg/100g), and saponins (106.59±1.09 mg/100g). Antioxidant screening showed 61.38% for DPPH and 64.85% for FRAP, while ascorbic acid showed 98.75% at 500 μg/ml. Physicochemical properties of the oils showed watermelon seed oil had a higher iodine value (171.07±1.514 mgl2/g) and acid value (6.59±0.01 mg/KOH/g), while orange seed oil had the highest peroxide value (1.82±0.01 mgl2/g). The composite gel showed viscosity of 86.10%, swelling capacity of 1.07%, and water absorbing capacity of 68.03%. A. flavus was more sensitive than A. niger, while P. aeruginosa was more sensitive than S. aureus. The study suggests that chitosan-Aloe vera extract gel with organic oils is an effective antimicrobial agent against pathogenic microorganisms. Chitosan-Aloe vera extract Organic oils antibacterial effects composite Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Under Review Version 1 posted Editorial decision: Revision requested 18 Mar, 2025 Editor assigned by journal 11 Mar, 2025 Submission checks completed at journal 11 Mar, 2025 First submitted to journal 07 Mar, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6177893","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":427125168,"identity":"b2a5d1bf-7e18-4ad3-a813-d06055c6c394","order_by":0,"name":"Ossamulu I. 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The phytochemical and antioxidant properties of the extract and the physicochemical properties of the oils were assessed. The antimicrobial activity was determined by the Minimum Fungicidal Concentration (MFC), Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC). The phytochemical analysis of Aloe vera extract revealed concentrations of alkaloids (29.72±0.65 mg/100g), tannins (142.03±0.99 mg/100g), flavonoids (113.82±1.83 mg/100g), phenols (565.68±1.36 mg/100g), and saponins (106.59±1.09 mg/100g). Antioxidant screening showed 61.38% for DPPH and 64.85% for FRAP, while ascorbic acid showed 98.75% at 500 μg/ml. Physicochemical properties of the oils showed watermelon seed oil had a higher iodine value (171.07±1.514 mgl2/g) and acid value (6.59±0.01 mg/KOH/g), while orange seed oil had the highest peroxide value (1.82±0.01 mgl2/g). The composite gel showed viscosity of 86.10%, swelling capacity of 1.07%, and water absorbing capacity of 68.03%. A. flavus was more sensitive than A. niger, while P. aeruginosa was more sensitive than S. aureus. 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