Unveiling Key Olfactory Receptors in Rhynchophorus ferrugineus Red Palm Weevil: A High-Throughput Structural Bioinformatics Approach

Unveiling Key Olfactory Receptors in Rhynchophorus ferrugineus Red Palm Weevil: A High-Throughput Structural Bioinformatics Approach | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 18 June 2025 V1 Latest version Share on Unveiling Key Olfactory Receptors in Rhynchophorus ferrugineus Red Palm Weevil: A High-Throughput Structural Bioinformatics Approach Authors : Rajeswari Kalepu , Maizom Hassan , Azzmer Azzar Abdul Hamid , Norfarhan Mohd- Assaad , and Nor Azlan Nor Muhammad 0000-0003-4401-0385 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.175028961.14336860/v1 253 views 105 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Olfactory receptor (OR) proteins play a crucial role in insect olfactory systems, including Rhynchophorus ferrugineus (red palm weevil), a significant global pest of palm trees. A scarcity of structural data on its chemosensory proteins, especially ORs, complicates the development of targeted control strategies. In the rapidly evolving field of protein structure analysis, identifying reliable reference proteins and obtaining precise structural data remain major challenges. This study utilised high-throughput structural bioinformatics to identify and characterize OR proteins in Rhynchophorus ferrugineus. Analysis of 110 OR proteins revealed that RferOR18148 and RferOrco were significant targets. A comparison of the modelled OR structures with experimental crystal structures confirmed their accuracy. Motif analysis indicated high conservation among the OR proteins, reflecting their evolutionary significance. Molecular dynamics (MD) simulations confirmed the structural stability of RferOR18148 and RferOrco , which maintain their native conformations in a lipid membrane. The literature study further confirmed that proteins are expressed in chemosensory systems, reinforcing their roles as reference structures. These protein structures have been successfully identified and validated for future research on OR and chemosensory mechanisms. The findings offer new insights into the molecular mechanisms of chemosensory signalling in R. ferrugineus , laying the groundwork for pest management strategies through the modulation of chemosensory pathways. Additionally, our research presents a systematic approach for identifying target proteins involved in olfactory communication, serving as a basis for future virtual screening studies of potential inhibitors. Supplementary Material File (or_manuscript 18 jun 2025.docx) Download 5.47 MB Information & Authors Information Version history V1 Version 1 18 June 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords rhynchophorus ferrugineus chemosensory proteins olfactory receptors red palm weevil reference structures structural bioinformatics target protein identification Authors Affiliations Rajeswari Kalepu Universiti Kebangsaan Malaysia Institut Biologi Sistem View all articles by this author Maizom Hassan Universiti Kebangsaan Malaysia Institut Biologi Sistem View all articles by this author Azzmer Azzar Abdul Hamid International Islamic University Malaysia Kulliyyah of Science View all articles by this author Norfarhan Mohd- Assaad Universiti Kebangsaan Malaysia Fakulti Sains dan Teknologi View all articles by this author Nor Azlan Nor Muhammad 0000-0003-4401-0385 [email protected] Universiti Kebangsaan Malaysia Institut Biologi Sistem View all articles by this author Metrics & Citations Metrics Article Usage 253 views 105 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Rajeswari Kalepu, Maizom Hassan, Azzmer Azzar Abdul Hamid, et al. Unveiling Key Olfactory Receptors in Rhynchophorus ferrugineus Red Palm Weevil: A High-Throughput Structural Bioinformatics Approach. Authorea . 18 June 2025. DOI: https://doi.org/10.22541/au.175028961.14336860/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); Cited by Rajeswari Kalepu, Azzmer Hamid, Maizom Hassan, Norfarhan Mohd-Assaad, Nor Muhammad, A Step-by-Step Computational Protocol for Functional Annotation and Structural Modelling of Insect Chemosensory Proteins, BIO-PROTOCOL, 15 , 1384, (2025). https://doi.org/10.21769/BioProtoc.5523 Crossref Loading... View Options View options PDF View PDF Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. 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