Occurrence of Antimicrobial Resistance, Integrons and Virulence Genes in Escherichia coli Isolated From Drinking Water in Van, Turkiye: A Cross-Sectional Study

Occurrence of Antimicrobial Resistance, Integrons and Virulence Genes in Escherichia coli Isolated From Drinking Water in Van, Turkiye: A Cross-Sectional Study | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Occurrence of Antimicrobial Resistance, Integrons and Virulence Genes in Escherichia coli Isolated From Drinking Water in Van, Turkiye: A Cross-Sectional Study Erva RAKICI, Elif AYDIN, Elif Seren TANRIVERDI, Osman Birol OZGUMUS This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5537694/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 31 May, 2025 Read the published version in BMC Microbiology → Version 1 posted 9 You are reading this latest preprint version Abstract Background Escherichia coli is one of the recommended freshwater indicator organisms for faecal contamination and can be associated with the pool of genes encoding antibiotic resistance. The aim of this study was to investigate the molecular characterisation of antibiotic resistance and to identify the virulence genes in E. coli isolated from drinking water samples collected for the purpose of monitoring microbial drinking water quality. Methods The E. coli isolates (n=244) from municipal tap water samples collected in Van province in Turkiye over a five-month period in 2018 were examined in this study. Antimicrobial susceptibility testing was performed using the standard disc diffusion method with 12 antibiotics. Integron content, the resistance genes such as blaTEM, blaSHV, blaCTX-M groups, tetA-E genes, qnrA, qnrB, qnrS, qepA, and aac(6’)-Ib-cr were screened and the virulence genes (afa, sfa, fimA, hly, iroN, aer, cnf, iutA and pap) were assessed by specific PCRs. The fingerprinting of the isolates harbouring the integron was evaluated using dendogram analysis of the band patterns obtained from the enterobacterial repetitive intergenic consensus (ERIC) PCR. Results Ampicillin, tetracycline, streptomycin, nalidixic acid and trimethoprim/sulfamethoxazole were the most common resistances. MAR index of 18.03% of the isolates was greater than 0.2. The two isolates produced ESBL. Thirty seven (15.16%) isolates carried the class 1 or class 2 integrons harboured the gene cassettes such as aadA, sat, estX and psp. Thirty two (21.47%) isolates were found to carry blaTEM, 5 (3.33%) isolates carried blaSHV and 2 (1.3%) isolates carried the blaCTX-M group genes. tetA (81.25%) and tetB (91.66%) were present in tetracycline-resistant isolates. qnrS (29.92%), qnrB and aac(6’)-Ib-cr (3.84%) were present in the nalidixic acid resistant isolates. Most of the isolates carried the virulence genes such as fimA, iutA, iroN, aer and sfa. Isolates belonged to 65.57% B1, 15.98% A, 9.42% D and 9.01% B2 phylogroups. A statistically significant difference was found between the phylogenetic groups of the isolates and the integron content (p<0.001). Fingerprinting showed the 25 different phylotypes in four clusters. Conclusions Molecular epidemiological findings in such a five-month period in the 11 sampled districts in the city of Van appearently showed us that the tap waters in public use was continuously contaminated by the antibiotic resistant and virulent E. coli isolates. This could pose a serious public health risk and, thus need the serious precautions for the infrastructures. Escherichia coli MAR index Virulence gene Integron Phylogenetic analysis Full Text Additional Declarations No competing interests reported. Supplementary Files Supplementarymaterial240325.pdf Cite Share Download PDF Status: Published Journal Publication published 31 May, 2025 Read the published version in BMC Microbiology → Version 1 posted Editorial decision: Revision requested 28 Apr, 2025 Editor assigned by journal 28 Apr, 2025 Reviews received at journal 15 Apr, 2025 Reviews received at journal 05 Apr, 2025 Reviewers agreed at journal 01 Apr, 2025 Reviewers agreed at journal 31 Mar, 2025 Reviewers invited by journal 30 Mar, 2025 Submission checks completed at journal 26 Mar, 2025 First submitted to journal 24 Mar, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5537694","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":436527036,"identity":"4c91a051-77b7-4f0c-9a0e-8d4baf506b7a","order_by":0,"name":"Erva 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The aim of this study was to investigate the molecular characterisation of antibiotic resistance and to identify the virulence genes in E. coli isolated from drinking water samples collected for the purpose of monitoring microbial drinking water quality.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethods\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe E. coli isolates (n=244) from municipal tap water samples collected in Van province in Turkiye over a five-month period in 2018 were examined in this study. Antimicrobial susceptibility testing was performed using the standard disc diffusion method with 12 antibiotics. Integron content, the resistance genes such as blaTEM, blaSHV, blaCTX-M groups, tetA-E genes, qnrA, qnrB, qnrS, qepA, and aac(6’)-Ib-cr were screened and the virulence genes (afa, sfa, fimA, hly, iroN, aer, cnf, iutA and pap) were assessed by specific PCRs. The fingerprinting of the isolates harbouring the integron was evaluated using dendogram analysis of the band patterns obtained from the enterobacterial repetitive intergenic consensus (ERIC) PCR.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAmpicillin, tetracycline, streptomycin, nalidixic acid and trimethoprim/sulfamethoxazole were the most common resistances. MAR index of 18.03% of the isolates was greater than 0.2. The two isolates produced ESBL. Thirty seven (15.16%) isolates carried the class 1 or class 2 integrons harboured the gene cassettes such as aadA, sat, estX and psp. Thirty two (21.47%) isolates were found to carry blaTEM, 5 (3.33%) isolates carried blaSHV and 2 (1.3%) isolates carried the blaCTX-M group genes. tetA (81.25%) and tetB (91.66%) were present in tetracycline-resistant isolates. qnrS (29.92%), qnrB and aac(6’)-Ib-cr (3.84%) were present in the nalidixic acid resistant isolates. 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