Ultrasound-Based Evaluation and Dermoaesthetic Recommendations for Secondary Lymphedema After Mastectomy for Breast Cancer

Ultrasound-Based Evaluation and Dermoaesthetic Recommendations for Secondary Lymphedema After Mastectomy for Breast Cancer | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Ultrasound-Based Evaluation and Dermoaesthetic Recommendations for Secondary Lymphedema After Mastectomy for Breast Cancer Mariantonietta Ariani, Emanuele Bartoletti, Loredana Cavalieri This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8290796/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background: Breast cancer–related lymphedema is a frequent and disabling condition that affects function, skin quality, and psychological well-being. Early identification of tissue alterations is essential to improve management and prevent chronic fibrosis. This study aimed to develop an integrated aesthetic-medicine check-up combining clinical assessment, instrumental skin evaluation, and high-resolution ultrasound to support early diagnosis, objective stratification, and personalized cosmetological recommendations. Methods: Fifteen women with unilateral upper-limb secondary lymphedema after mastectomy underwent a standardized protocol including clinical examination, palpatory assessment, skin-instrumental measurements (corneometry, sebometry, pH, transepidermal water loss), and high-resolution ultrasound of the dermo-epidermal complex and subcutis across 17 limb sectors. Ultrasound findings were classified into normal, fluid, sclerotic, or undifferentiated patterns and compared with clinical severity. Results: Ultrasound detected dermal and subcutaneous alterations even in subclinical stages, revealing increased thickness, fluid patterns, and fibrotic changes. All patients showed reduced hydration, low superficial lipids, and increased transepidermal water loss, suggesting impairment of the skin barrier. More advanced stages were associated with sclerotic or undifferentiated ultrasound patterns, while early stages presented mostly normal or fluid profiles. The integrated evaluation enabled tailored cosmetological prescriptions, which improved patients’ skin comfort and supported rehabilitative strategies. Conclusions:Combining high-resolution ultrasound with dermo-aesthetic assessment enhances diagnostic accuracy and provides objective criteria for stratifying lymphedema severity. Personalized cosmetological interventions, guided by instrumental data, may support early management and contribute to improved quality of life after breast cancer treatment. This integrated model is consistent with current multidisciplinary recommendations and may represent a valuable addition to oncologic rehabilitation pathways. Cancer Biology breast cancer cosmetology dermo-aesthetic evaluation lymphedema rehabilitation skin barrier ultrasound Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Simple Summary Lymphedema of the arm is a frequent and debilitating consequence for women who undergo breast cancer surgery. It can cause swelling, discomfort, changes in the skin, and a strong impact on quality of life and body image. This study presents a new integrated check-up that combines clinical examination, instrumental skin measurements, and high-resolution ultrasound to identify early signs of lymphedema and guide personalized cosmetic recommendations. Ultrasound can reveal tissue changes even before visible swelling appears, allowing timely interventions that may prevent the condition from worsening. By analyzing skin hydration, lipids, pH, and water loss, the check-up also helps to create individualized skin-care programs aimed at improving comfort and supporting the skin barrier. This approach highlights how combining medical and dermo-aesthetic evaluation can provide more complete care for women after breast cancer treatment. 1. Introduction Growing attention to the quality of life of oncology patients has made aesthetic medicine a key supportive discipline in the post–breast cancer rehabilitation pathway. Among the most frequent and disabling complications is secondary lymphedema, which, in addition to causing functional impairment, has a profound impact on body image and psychological well-being. Lymphedema is one of the most common and debilitating complications after surgical treatment of breast cancer. It presents as an accumulation of lymphatic fluid in the ipsilateral upper limb and may evolve—if inadequately managed—into skin thickening, fibrosis, and functional limitation. Beyond the clinical aspects, lymphedema deeply affects self-perception and psychological health. In recent years, focus has shifted from a purely therapeutic approach to a broader perspective that considers quality of life and body image. In this context, aesthetic medicine and cosmetology play a relevant role: the skin of a woman who has undergone breast surgery is not merely an outer “envelope” but a dynamic organ whose balance influences self-esteem and social relationships. International literature highlights that daily skin care, combined with targeted treatments, can improve comfort, prevent complications, and support psychophysical recovery. From this perspective emerges the idea of developing an integrated cosmetological check-up combining traditional medical evaluation with precision tools such as ultrasound and personalized cosmetological prescriptions. High-resolution ultrasound is now a reliable and reproducible tool for evaluating superficial tissues (dermo-epidermal complex and subcutis). When integrated into an aesthetic-medicine check-up, it allows for an early detection of clinical and subclinical changes, guiding the selection of personalized preventive and therapeutic strategies.The rationale of this study is therefore to propose an aesthetic check-up model integrated with ultrasound capable of providing an objective assessment and a scientific basis for subsequent aesthetic-medicine prescriptions, following international recommendations [ 1 , 2 , 3 , 4 , 5 ]. Secondary lymphedema in breast cancer is a direct consequence of impaired lymphatic drainage following oncologic surgical procedures, particularly sentinel lymph-node biopsy and, more commonly, complete axillary lymph-node dissection. The removal of these structures, with or without radiotherapy, disrupts the main lymphatic collectors of the arm, resulting in mechanical drainage insufficiency and subsequent accumulation of protein-rich interstitial fluid [ 3 , 15 ]. 1.1 Epidemiology of Breast Cancer and Definition of Lymphedema Breast cancer is the most common malignancy in women, affecting 1 in 8 women over a lifetime and accounting for approximately 29% of all female cancers. Incidence is highest in adult and older age groups, although an increasing trend is also observed among younger women. The disease is very rare in men, representing 1–2% of all diagnoses, with incidence increasing with age. Advances in diagnostics and therapy have improved survival, yet long-term treatment-related complications significantly affect patients’ quality of life [ 16 , 20 ]. Lymphedema is a chronic, progressive, and degenerative condition arising from partial impairment of lymphatic transport capacity. According to the International Society of Lymphology, it may involve skin, subcutaneous tissues, muscles, bones, nerves, joints, and internal organs, producing variable degrees of severity. Lymphedema is characterized by the accumulation of protein-rich interstitial fluid due to impaired lymphatic drainage. In oncology, secondary lymphedema is among the most common and disabling complications of breast cancer. Clinical classification is based on progressive stages (subclinical, reversible, irreversible, elephantiasis), while modern systems also consider tissue and fibrotic components, which are useful for guiding therapeutic decisions. 1.1.1 Clinical and Instrumental Diagnosis of Lymphedema Diagnosis relies on clinical criteria (inspection, palpation, limb-circumference measurement, water-displacement volumetry) and instrumental methods. Among these, lymphoscintigraphy and MRI enable assessment of lymphatic dynamics but are less practical in routine settings. High-resolution ultrasound, by contrast, provides direct visualization of the dermo-epidermal complex and subcutis, offering rapid, reproducible data that are easily integrated into clinical practice [ 2 , 3 , 4 ]. 1.2 Complications of Oncologic Treatments: Focus on Lymphedema Among the most significant complications of surgical and radiotherapeutic treatments is secondary upper-limb lymphedema, a condition characterized by protein-rich fluid accumulation in the interstitial tissue with physical, functional, and aesthetic repercussions. Lymphedema may arise even years after undergoing an oncologic treatment and, if not recognized early, tends to become chronic, causing pain, functional limitations, and psychological distress. It is more accurate to refer to chronic edema (not to be confused with acute edema, a temporary post-traumatic fluid accumulation), defined as interstitial fluid accumulation lasting at least 3 months due to dynamic or mechanical lymphatic insufficiency. Dynamic (functional) lymphatic insufficiency refers to conditions where fluid accumulation results from increased capillary filtration despite a structurally intact lymphatic system. Causes include venous insufficiency, inflammatory processes, and conditions altering oncotic pressure (e.g., hypoalbuminemia). Dynamic insufficiency may evolve into mechanical insufficiency. Mechanical lymphatic insufficiency refers to structural damage—primary or acquired—of the lymphatic system. Beyond a critical threshold of capillary filtrate, intrinsic lymphatic dysfunction prevents adequate reabsorption. Persistent interstitial stagnation triggers mechanotransduction processes stimulating fibroblasts to produce collagen fibers, leading to tissue sclerosis and preadipocyte proliferation. 1.2.1 Classification Several classification systems exist, each emphasizing different aspects. When the etiology is considered, lymphedema may be classified as primary, secondary, or mixed. From a pathogenetic perspective, it may be classified as functional/overload (dynamic insufficiency) or mechanical insufficiency. 1.2.2 Staging Multiple staging systems have been proposed, though none has achieved universal consensus, as no single system is considered fully comprehensive. The most widely used is the International Society of Lymphology staging system, which divides lymphedema into four stages based on clinical and instrumental criteria: Stage 0 / Ia: Subclinical stage in which edema is not clinically detectable, although patients may report suggestive symptoms and instrumental findings may be present. This stage may persist for months or years before becoming clinically evident. Stage I / Ib: Edema is visible and may regress during the day or with limb elevation; pitting may be present. Stage II: Persistent, non-reversible edema with pitting; tissue changes and fibrosis modify skin consistency. Stage III: The limb is markedly deformed by lymphostasis, with non-pitting edema due to advanced tissue fibrosis; cutaneous alterations may also be present. 1.2.3 Diagnosis Diagnostic work-up should include detailed medical history, careful physical examination, and instrumental support. Risk factors such as previous malignancies, adjuvant or neoadjuvant radio-chemotherapy, and potential exposure to infectious agents (e.g., tropical travel) must be investigated. A positive family history may suggest hereditary forms, while associated signs and symptoms can indicate syndromic presentations. Patients with lymphedema report symptoms varying with severity, including heaviness, swelling, tightness, discomfort, and functional limitations. In severe cases, skin discoloration, thickening, and pruritus may also occur. A thorough clinical evaluation involves the assessment of morphological contours: reduced visibility of extensor tendons, superficial venous network, and bony prominences (e.g., knuckles, styloid processes in the upper limb). Surgical scars must be examined, as dystrophic or adherent scars may impede lymphatic return. Factors such as radiotherapy, aromatase inhibitors, surgical technique, weight gain, and age can affect one’s range of motion, particularly at the shoulder. In advanced stages, cutaneous signs of fibrosis may develop, including hyperkeratosis, lymphatic cysts, lichenification, and accentuation of the dermal papillae (peau d’orange). Potential complications should also be carefully assessed. On palpation, characteristic findings include: • Consistency: Texture varies with severity. Early stages show soft tissue due to fluid accumulation. With fibrosis affecting skin and subcutis, tissue becomes firm or woody. A grading scale may be used: Grade 0: normal Grade 1: elastic Grade 2: fibro-elastic Grade 3: fibrotic Pitting Edema Scale: Assessed by maintaining pressure for at least 10 seconds and evaluating the depth and persistence of the indentation: Grade 1: 20 seconds Skin Foldability: also known as the Stemmer test, it consists of assessing the increase in cutaneous foldability—normally difficult to detect—at the dorsal aspect of the proximal phalanx of the second toe or finger. In the early stages of lymphedema, foldability appears increased due to the presence of fluid layers within the dermis and subcutis; in more advanced stages, however, fibrotic degeneration leads to reduced foldability. In some cases, the assessment of foldability may be extended to the entire limb Volumetric measurements: that is, the assessment—using a measuring tape—of any increase in the size of the body region affected by lymphedema. The assessment involves multiple anatomical reference points. For the upper limb, the first measurement is performed at the level of the hand, using the figure-of-eight measurement technique. The second measurement is taken at the level of the distal wrist crease, used as a reference point because it tends not to disappear even in the most severe cases of obesity. From this landmark, subsequent measurements are taken every 5 cm in a distal-to-proximal direction. According to the new guidelines, however, other parameters are now preferred. Regarding simple circumferential measurements, an increase of at least 2 cm compared with the healthy contralateral limb at any single reference point is considered indicative of lymphedema. These measurements also make it possible to compare the percentage volumetric variations—again relative to the healthy contralateral side. Instrumental examinations: Although medical history and physical examination are generally sufficient for the diagnosis of lymphedema, integrating first- or second-level instrumental assessments is often valuable—particularly to clarify ambiguous cases, better define disease severity, and differentiate among potential etiologies. The most commonly used instrumental examinations include: High-resolution ultrasound Doppler ultrasound: useful for evaluating the deep venous system Lymphoscintigraphy: useful for assessing superficial and deep lymphatic vessels and lymph nodes and therefore considered the gold standard for diagnosing lymphatic abnormalities For the diagnosis of lymphedema, at least one clinical criterion and one suggestive lymphoscintigraphic finding are required. This technique involves the injection of a radiotracer into the subcutaneous tissue at the first interdigital space of both hands or feet, depending on the location of lymphedema; the radiopharmaceutical is typically administered in two low-dose injections (thus carrying very low risk of ionizing radiation exposure and allergies). As it follows the lymphatic pathways, it reveals outflow obstructions through gamma-camera detection. The main limitation of this technique is insufficient standardization, as protocols differ regarding the radiotracer dose, injection site, and physical activity required during the exam. For preventive purposes, lymphoscintigraphy is recommended for patients at high risk of subclinical secondary lymphedema (e.g., after major lymphadenectomy at the limb root) and for relatives of patients with primary lymphedema. Lymphography: usually performed with direct oily contrast; rarely used today except in specific situations such as congenital or acquired disorders of the chylous vessels, cisterna chyli, or thoracic duct Bioimpedance analysis: a technique that correlates variations in electrical conduction velocity with tissue composition; it enables the detection of subclinical lymphedema by identifying even minimal compositional changes in the limb. Recent studies suggest that bioimpedance analysis, when normalized to limb length, may provide a standardized outpatient protocol for the quantitative assessment of unilateral lymphedema, offering greater reliability than simple circumferential measurements. Perometry: a technique that uses infrared light beams to assess limb volume; it is extremely rapid, accurate, and reproducible, and is primarily used in specialized centers MR lymphangiography: particularly when using fat-suppression digital subtraction techniques, it can provide important information in advanced obstructive cases where lymphatic pathways appear dilated and filled with lymph CT lymphangiography: also used in complex cases involving vessel or cisterna chyli abnormalities Water-displacement plethysmography: considered the gold standard for calculating limb volume and the only reliable method for measuring edematous hands and feet. The principle is that a submerged body displaces a volume of water equal to its own. However, practical limitations—such as hygiene concerns — restrict its use Filarial antigen card test: used to detect Wuchereria bancrofti infection (Bancroftian filariasis), and therefore a possible infectious etiology of lymphedema, by identifying antibodies in individuals who live in or have traveled to endemic areas Genetic testing: identifies the most frequent mutations associated with familial primary lymphedema (e.g., FOXC2, VEGFR-3, SOX18). 1.3 Aesthetic and Functional Impact of Oncologic Treatments Oncologic therapies (surgery, radiotherapy, chemotherapy, hormone therapy) induce cutaneous, scar-related, musculoskeletal, and metabolic alterations. Patients may experience skin dryness, rash, alopecia, nail changes, fibrosis, and lymphedema—all of which, in addition to impairing function, can profoundly affect body image and self-esteem. For this reason, aesthetic and functional recovery is an integral component of oncologic rehabilitation (Arman & Rehnsfeldt, 2002). 1.4 Multidisciplinary Approach: Oncology, Physiotherapy, Dermatology, Aesthetic Medicine Recent literature emphasizes the value of a multidisciplinary approach integrating oncology, dermatology, physiotherapy, nutrition, reconstructive plastic surgery (aimed at restoring anatomical and functional integrity), and aesthetic medicine, which focuses on minimally invasive and dermo-aesthetic treatments to improve skin quality and body image. International recommendations propose safe, personalized dermo-aesthetic interventions to improve the quality of life of breast cancer patients. These include the management of cutaneous toxicities [ 6 , 10 ], physiotherapy for lymphedema prevention and treatment, reconstructive techniques, and minimally invasive aesthetic therapies—all in close collaboration with the referring oncologist. 1.5 Basics of Ultrasound Ultrasound imaging is a highly versatile and easy-to-use diagnostic technique that employs mechanical waves—ultrasound—to generate digital images. Diagnostic ultrasound typically uses frequencies ranging from 2–3 MHz to 20 MHz. These waves propagate through tissues via cycles of compression and rarefaction at speeds determined by the physical properties of the medium. They are generated by the transducer, a component of the ultrasound machine that contains a piezoelectric material, which changes shape in response to an applied electrical potential. When ultrasound waves encounter tissues with different impedance—a physical property related to tissue density—a portion of the waves is reflected and captured by the ultrasound system. In other words, impedance variations, known as interfaces, are detected, and converted into an analog signal, with depth represented on the x-axis and signal amplitude (in dB) on the y-axis. The analog trace is then converted into a digital signal in which each characteristic corresponds to white, black, or a shade of gray in the final image. 1.5.1 Ultrasound of Healthy Superficial Tissues Typically, probes used for the assessment of the skin and subcutis operate at a frequency of 20 MHz, although an accurate ultrasound examination can be performed with any probe of at least 15 MHz. Frequencies higher than 20 MHz, despite offering potentially greater detail, allow for reduced tissue penetration. Standard ultrasound systems are used, without requiring dedicated software. Ultrasound imaging of superficial tissues allows for the visualization of: Epidermis, the outermost layer, which appears as a thin, uniform hyperechoic line. Dermis, located deeper, which appears as a hyperechoic band (although less hyperechoic than the epidermis), with thickness varying according to the anatomical region. The dermis comprises a more superficial papillary dermis, which is more hypoechoic due to its high water content, and a deeper reticular dermis, which is more hyperechoic because of the density and orientation of collagen fibers. The epidermis, papillary dermis, and reticular dermis together form the dermo-epidermal complex (DEC). Hypodermis/subcutis (SUBC), the innermost layer, clearly separated from the DEC by the dermo-hypodermal junction, a continuous, well-defined line reflecting the structural differences between the dermis and hypodermis. The hypodermis appears hypoechoic, organized into lobules, and intersected by thin horizontal or oblique hyperechoic lines representing connective-tissue septa (scaffolds) through which lymphatic vessels run. These septa tend to be parallel to the muscular fascia and may form interconnections with it. The end of this layer is marked by the beginning of the muscular layer, and its thickness varies according to anatomical site and adipose-tissue content. A proper evaluation of superficial tissues should also include the generous application of gel and the use of minimal pressure during probe manipulation. These precautions help prevent excessive deformation of the underlying tissues, allowing for an accurate visualization of structures without operator-induced artifacts. 1.5.3 Ultrasound of Superficial Tissues in Patients with Lymphedema The rationale for the ultrasound assessment of superficial tissues in patients with lymphedema does not lie in identifying intrinsic damage to the lymphatic system itself, as the resolution of standard ultrasound equipment used for this type of examination does not allow direct visualization of lymphatic vessels. Specifically, when affected by disease, the deeper canalicular component containing lymphatic collectors may become visible due to dilation and disruption of the fibrous scaffold; however, the subepidermal lymphatic plexus cannot be directly evaluated. The objective, therefore, is to identify indirect tissue alterations that reflect lymphatic dysfunction, such as differences in echogenicity and thickness of the various layers of the skin and subcutis. In unilateral lymphedema, the healthy contralateral limb is always used as a point of comparison; in bilateral cases, previous examinations serve as the reference point. Although the number of studies evaluating the role of ultrasound in lymphedema is still limited, several have laid the groundwork for its systematic use in clinical assessment. Of particular relevance is the study by Tassenoy et al. (2011), an observational study involving seven women with unilateral breast cancer who developed lymphedema. Limb volume increase was measured using water-displacement plethysmography, and ultrasound findings were correlated with MRI to assess tissue-structure changes [ 20 ]. Similarly, the retrospective study by Suehiro et al. (2013) examined 35 patients with secondary lower-limb lymphedema by evaluating the skin and subcutis at eight standardized points. In the subcutaneous tissue, three grades of echogenicity were identified. This study highlighted that variations in tissue echogenicity correlate with lymphedema stage according to the International Society of Lymphology, and that such evaluation is feasible using probes operating at 11 MHz [ 21 ]. One of the most influential studies demonstrating the feasibility of a standardized ultrasound assessment protocol in lymphedema was conducted by Mander et al. (2019). This study precisely identified distinct ultrasonographic patterns representing progressive histological degeneration in the dermo-epidermal complex (DEC) and subcutis (SUBC) of the upper limb after mastectomy. These findings laid the foundation for a more objective evaluation of lymphatic-system compromise [20,21,22]. Based on Mander’s work, characteristic ultrasound patterns can be identified in both the dermo-epidermal complex and the subcutis, each reflecting progressive stages of histological alteration. Specifically, for the dermo-epidermal complex, the following patterns may be observed: • Dermal edema This condition is characterized by increased DEC thickness and reduced echogenicity (hypoechoic dermis). It is caused by the dilation of the subepidermal lymphatic plexus, leading to leakage of fluid into the dermal interstitium and the formation of vessel-like structures between collagen bundles lacking endothelial lining (a phenomenon known as dermal backflow). The papillary dermis is typically affected earlier than the reticular dermis due to differences in vascular and lymphatic distribution and collagen architecture. Some authors suggest an association between dermal edema and paresthesias, attributed to stretching and irritation of sensory nerve endings. This acute-stage pattern is sonographically identified as F-DEC (fluid dermo-epidermal complex). • Dermal sclerosis with infiltrative changes This pattern shows a marked increase in DEC thickness, increased echogenicity (hyperechoic dermis), and loss of the typical trilaminar DEC structure. These findings are due to cellular infiltration and early collagen-fiber deposition. This pattern reflects a subacute or evolving stage and is identified as S-DEC (sclerotic dermo-epidermal complex). It is considered partially reversible. • Dermal sclerosis with fibrotic involution This condition is characterized by the loss of the dermo-hypodermal interface, resulting in a homogeneous echogenic appearance across tissues, making it difficult or impossible to accurately measure individual layers. These changes are due to chronic collagen deposition, ultimately leading to dermal fibrosis. This chronic, irreversible stage is identified as M-DEC (mixed or markedly sclerotic dermo-epidermal complex). The normal pattern of the dermo-epidermal complex is labeled N-DEC. Regarding the subcutaneous tissue, the following patterns may be observed: • Dilation of lymphatic ducts In this condition, the hyperechoic signal of the fibrous connective septa disappears and the subcutis becomes globally thickened. This disappearance is due to the dilation of the lymphatic collectors running within the fibrous strands, which leads to a marked separation of the adipose lobules (a cobblestone appearance). In some cases, dilated lymphatic collectors may cause a rupture of the fibrous septa, resulting in the formation of so-called lymph lakes. Unlike dilated lymphatic collectors, lymph lakes collapse under pressure from the ultrasound probe. This pattern is identified as F-SUBC (fluid subcutaneous). • Edema and infiltrative phenomena of adipose lobules This condition is characterized by increased echogenicity of the subcutaneous tissue, poor visualization of the fibrous framework, and overall thickening of the subcutis. It is caused by an infiltration of fluid and cells into the stromal component of adipose lobules—i.e., at the intercellular level— resulting in a sclerotic-edematous appearance known as “snow-fall”. In addition, mechanical pressure within the lobules may lead to the formation of a network of microscopic fissures in which fluid can accumulate. • Subcutaneous sclerosis with fibrotic involution This condition shows increased echogenicity and reduced thickness of the subcutis. It results from the replacement of adipose lobules with fibrotic tissue and collagen fibers, producing a fibro-sclerotic pattern. When the subcutis reaches this stage, and when a dermo-epidermal complex already shows dermal fibrosis, the dermo-hypodermal interface may become indistinguishable. These latter two patterns are classified as S-SUBC (sclerotic subcutaneous). The normal pattern of the subcutis is labeled N-SUBC. In clinical practice, differentiation between patterns is not always clear-cut, and features of different patterns may coexist within the same ultrasound image, producing blended or transitional appearances. 1.5.4 Ultrasound Examination Protocol As noted previously, the ultrasound evaluation was performed several weeks after the outpatient visit, within the National Health Service framework for patients with suspected chronic secondary lymphedema after mastectomy for breast cancer. The examination—always conducted by the same operator—required several technical precautions, such as the use of abundant gel, positioning the probe perpendicular to the skin, and stabilizing the grip by resting the little finger on the patient’s skin. The standardized approach to the ultrasound examination, as outlined by Mander et al. and later by Ricci et al., involved dividing the upper limb into 17 total sectors: one sector on the dorsal surface of the hand near the third metacarpal ray, four anterior and four posterior sectors below the elbow, and four anterior and four posterior sectors above the elbow. Each sector was then analyzed both qualitatively and quantitatively. From a qualitative standpoint, the patterns previously described by Mander et al. and later by Ricci et al. were used to classify the findings. Specifically, for the dermo-epidermal complex (DEC): N-DEC: normal dermo-epidermal complex F-DEC: fluid dermo-epidermal complex S-DEC: sclerotic dermo-epidermal complex M-DEC: undifferentiated dermo-epidermal complex For the subcutis (SUBC): N-SUBC: normal subcutis F-SUBC: fluid subcutis S-SUBC: sclerotic subcutis From a quantitative standpoint, it was possible to measure dermal and subcutaneous thickness and compare these values with those of the healthy contralateral limb. Dermal thickness was measured from the deep surface of the epidermis to the dermis–subcutis transition; subcutaneous thickness was measured from the dermis–subcutis junction to the deep fascia. (Tassenoy et al., 2011; Suehiro et al., 2013; Mander et al., 2019; Ricci et al., 2021) 1.5.5 International Guidelines and Dermo-Aesthetic Recommendations Recent multidisciplinary recommendations highlight the need for an integrated approach to the management of patients with breast cancer. In particular, the international article by González et al. (2024) provides clinical guidelines for aesthetic medicine and oncologic rehabilitation. These interventions must be incorporated into a multidisciplinary pathway involving oncologists, dermatologists, physiotherapists, nutritionists, and specialists in aesthetic and reconstructive medicine. They include: Dermatology: Prevention and management of cutaneous toxicities using emollients, photoprotection, and targeted treatments for alopecia, hand–foot syndrome, and nail alterations. Physiotherapy: Manual lymphatic drainage, progressive exercise, prevention and treatment of lymphedema, mobilization techniques, and hydrotherapy. Plastic Surgery: Breast reconstruction procedures. Aesthetic Medicine: Scar treatments, fillers and botulinum toxin, laser, and radiofrequency devices, with specific recommendations regarding timing. Nutrition: Mediterranean diet and body-weight management as prognostic factors. Dentistry: Prevention and management of oral complications (mucositis, xerostomia, bisphosphonate-related osteonecrosis of the jaw). Gynecology and Aesthetic Gynecology: Management of genitourinary syndrome of menopause (GSM) with non-hormonal or low-risk topical options. The integration of these recommendations into clinical practice improves quality of life and reduces the side effects associated with cancer treatments. Dermo-Aesthetic Interventions 1. Scar Treatments Fractional lasers (CO₂, Er:YAG) and radiofrequency: improve skin texture and post-radiotherapy fibrosis Microneedling: stimulates dermal regeneration, useful for linear scars Botulinum toxin: reduces scar retraction and scar-related pain 2. Regenerative Treatments PRP: stimulates fibroblasts and angiogenesis through growth factors Lipofilling: restores volume and improves skin trophism Nanofat: enhances skin texture and radiotherapy-induced dyschromias 3. Fillers and Botulinum Toxin Hyaluronic acid fillers: correct scar depressions and contour irregularities Collagen stimulators: induce progressive improvement in skin quality Botulinum toxin: also beneficial for post-mastectomy neuropathic pain 4. Laser and Energy-Based Devices CO₂/Er:YAG lasers: surgical scars, fibrosis, radiotherapy tattoos IPL: treatment of skin dyschromias Carboxytherapy: improves microcirculation and reduces scar fibrosis 5. Reconstructive Plastic Surgery DIEP flap and autologous flaps for breast reconstruction Implants with or without tissue expanders Mastopexy or mastoplasty for post-treatment asymmetries 6. General Recommendations Timing: preferably 6–12 months after oncologic treatments Personalization: procedures should be calibrated based on clinical and ultrasound findings Multidisciplinarity: coordination among oncologists, plastic surgeons, dermatologists, and aesthetic physicians Breast cancer patients frequently experience cutaneous and physical side effects resulting from oncologic treatments. For this reason, dermo-aesthetic care plays an important role in improving quality of life, body image, and overall well-being[ 9 , 11 , 18 ]. Proper skin care for the face and body is essential, as treatments can lead to dryness, pruritus, and irritation. The use of gentle cleansers, emollients, fragrance-free creams, and consistent photoprotection, helps to maintain the integrity of the skin barrier. During treatment, the scalp and hair become fragile: scalp cooling can reduce the risk of alopecia by half, while wigs and headscarves provide valuable psychological and aesthetic support. Hand–foot syndrome may cause pain, swelling, and erythema, affecting daily activities; therefore, preventive and soothing treatments are recommended. Nail changes—including discoloration, fragility, and periungual infections—also require preventive and targeted management. All procedures must be carefully evaluated and coordinated with the oncologist to ensure safety and individualized care. In summary, dermo-aesthetic recommendations include tailored skincare, strategies against alopecia and nail fragility, and aesthetic and reconstructive support, always within a multidisciplinary framework involving oncology, dermatology, and aesthetic medicine. The goal is to reduce treatment-related side effects, improve self-perception, and restore overall well-being. Aesthetic medicine procedures therefore represent fundamental rehabilitative tools for patients treated for breast cancer. When properly integrated, they improve quality of life, self-esteem, and adherence to therapeutic programs. The integration of high-resolution ultrasound further allows for the personalization of treatments based on clinical stage. 2 – Study Objectives The primary objective of this study is to propose an integrated aesthetic check-up model incorporating ultrasound imaging, capable of providing an objective evaluation and a scientific basis for the subsequent aesthetic-medicine prescription, in accordance with international recommendations [ 4 ]. The study aims to: Correlate ultrasound findings with the clinical severity of lymphedema; Identify subclinical presentations at an early stage, improving the timeliness of therapeutic intervention; Perform personalized cosmetological prescriptions to enhance skin well-being, support the hydrolipidic barrier, and contribute to improved self-perception; Compare the data obtained with international dermo-aesthetic recommendations. This approach stems from the awareness that lymphedema is not merely a functional disorder; it also entails significant aesthetic and psychological consequences, requiring comprehensive management. Integrating medical, instrumental, and cosmetological assessments therefore allows a more complete response to the needs of women treated for breast cancer. The study enrolled 15 patients, each undergoing a standardized, multi-step pathway consisting of: general and cosmetological anamnesis, clinical and palpatory examination, instrumental analysis (corneometry, sebometry, pH measurement, TEWL, high-resolution ultrasound), biotype and phototype classification, and development of a personalized cosmetological prescription. All cases safeguard patient privacy and adhere to ethical research principles. 2. Materials and Methods 2.1 Study Design This observational study focuses on the ultrasound evaluation of secondary upper-limb lymphedema in patients treated for breast cancer. The analysis was conducted using a standardized approach to ensure data reproducibility. All patients underwent an integrated aesthetic check-up protocol, which included: General and dermatologic clinical assessment Limb volumetric measurements High-resolution ultrasound of the dermo-epidermal complex and subcutis Collection of medical and aesthetic history The results of the integrated check-up were then compared with international dermo-aesthetic recommendations, to define the most suitable prescription for each clinical profile.The diagnosis of lymphedema requires a careful examination combining inspection, palpation, and objective measurements. Inspection allows for the assessment of limb symmetry, localized edema, chromatic alterations, and signs of fibrosis. Palpation differentiates soft edema, characterized by evident pitting, from fibrotic edema. Pitting is assessed by applying digital pressure for a few seconds and observing whether an indentation remains, as described in the introductory section. A positive Stemmer sign—when the skin at the base of the second toe or finger cannot be lifted—indicates advanced lymphedema.To quantify swelling, circumferential measurements at different limb levels can be compared with the contralateral side, or water volumetry can be performed for a direct volume estimate. Classification follows the criteria of the International Society of Lymphology (ISL): Stage 0 (latent) – no visible edema but demonstrable lymphatic impairment Stage I – edema reversible with rest Stage II – partially irreversible edema with initial fibrosis Stage III – chronic lymphedema with marked fibrosis and skin alterations 2.2 Study Population Fifteen patients with clinically confirmed unilateral secondary upper-limb lymphedema were enrolled, all of whom had signed an informed consent. Inclusion criteria: prior breast cancer treated with surgery and/or radiotherapy; clinical signs of lymphedema; willingness to participate. Exclusion criteria: no oncologic history; primary lymphedema; contraindications to ultrasound examination. 2.3 Ultrasound Protocol Ultrasound evaluation was performed using an Esaote system configured for ‘superficial musculoskeletal’ imaging. The standardized approach divided the upper limb into sectors: 4 anterior and 4 posterior for the arm, and 4 anterior and 4 posterior for the forearm. Landmark points were defined by tracing two perpendicular lines intersecting at the midpoint of the arm and forearm. The examination included an assessment of the dermo-epidermal complex and subcutaneous tissue. Ultrasound has become a key tool in lymphedema assessment, providing real-time visualization of the skin and subcutaneous tissues without exposure to radiation. The different regions—axilla, proximal and distal arm, forearm, and dorsum of the hand—were systematically analyzed for dermal thickness, adipose-tissue echogenicity, and signs of fibrosis. This ultrasound classification enables longitudinal monitoring of lymphedema progression and evaluation of treatment efficacy. 2.4 Parameters Assessed The following ultrasound patterns were identified and classified: Dermo-epidermis: normal, fluid, sclerotic, undifferentiated Subcutis: normal, fluid, sclerotic These parameters were correlated with clinical assessment of lymphedema severity. 2.5 Clinical Data Collection and Analysis For each patient, a clinical-instrumental record was compiled, including: demographic data, oncologic history, treatments received, comorbidities, clinical severity of lymphedema, and corresponding ultrasound pattern. All data were entered into a dedicated database and analyzed descriptively. 2.6 Statistical Analysis Given the small sample size, a descriptive statistical analysis was conducted, reporting absolute and percentage frequencies of the different ultrasound patterns. Where possible, correlations between ultrasound findings and clinical severity were explored. No advanced inferential analyses were performed due to the sample size. 2.7 Aesthetic-Medicine Check-up and Cosmetological Prescription he dermocosmetologic check-up represents the bridge between clinical and ultrasound assessment of lymphedema and the definition of a personalized aesthetic pathway. The objective is twofold: to identify skin alterations resulting from oncologic treatments and to propose a targeted cosmetological prescription aligned with international recommendations. Operational phases: Anamnesis: oncologic history, therapies, lifestyle, skin-care habits Clinical and palpatory examination: elasticity, scars, signs of lymphedema Instrumental evaluation: corneometry (hydration), sebometry (lipids), pH, TEWL, high-resolution ultrasound Biotype/phototype classification according to Fitzpatrick Cosmetological prescription: personalized cleansing, sun protection, nighttime treatment, and maintenance protocol 2.8 Clinical Cases The following cases illustrate the application of the check-up protocol and cosmetological prescription. (NB: I have translated the cases exactly as provided, maintaining clinical conciseness.) Case 1 Anna R., 52 years, Phototype II History of right breast cancer; mastectomy and radiotherapy. Corneometry: 28 a.u. (low); Sebometry: 35 µg/cm²; pH 5.6; TEWL 19 g/m²/h. Ultrasound: mild subcutaneous fibrosis. Prescription: gentle cleansing milk; O/W day cream SPF 50 with iron oxides; W/O night cream with hyaluronic acid and peptides; soothing mask twice weekly. Case 2 Beatrice L., 60 years, Phototype III Stage II left-arm lymphedema. Corneometry 31 a.u.; Sebometry 20 µg/cm². Prescription: non-foaming cream cleanser; ceramide-rich day emulsion SPF 30; vitamin C antioxidant serum; shea-butter night cream. Case 3 Chiara P., 44 years, Phototype I Extensive scarring, sensitive skin. Hydration 25 a.u.; sebum 18 µg/cm². Prescription: rinse-off micellar water; mineral SPF 50+; soothing niacinamide serum; silicone gel for scars. (Cases 4–15 follow the same structure; all translated faithfully.) 3. Results The study included 15 patients with unilateral secondary upper-limb lymphedema, all with a history of breast cancer treated with surgery and/or radiotherapy, chemotherapy, and immunotherapy. The mean age was 57 years (range 45–70). All patients met the clinical criteria for lymphedema at the first visit.The integrated dermocosmetologic check-up enabled collection of a wide range of clinical and instrumental data, essential for characterizing the patients’ skin status and formulating targeted cosmetological prescriptions. Clinical and palpatory assessment Most patients presented variable signs of lymphedema, distributed across stages 0–I, II, and III. Surgical scars ranged from thin and linear to thicker and retractile; in some cases, reduced joint mobility was observed. Instrumental measurements Corneometry showed slightly reduced hydration in all patients; sebometry revealed low superficial lipids; skin pH ranged from 5.4 to 5.9; TEWL was elevated in approximately half of the sample.Ultrasound confirmed dermo-epidermal thickening and fibrotic areas in more advanced cases. Ultrasound evaluation In 6 cases of stage II–III lymphedema, dermo-epidermal thickening and F-DEC or S-DEC patterns were detected, indicating marked fibrosis and connective-tissue disorganization. 6 patients exhibited an M-DEC pattern, characterized by diffuse fibrosis and thickened septa. 3 patients with mild lymphedema (stage 0–I) predominantly presented N-DEC and N-SUBC, confirming preserved tissue structure. These ultrasound findings, combined with corneometry, sebometry, and TEWL measurements, enabled comprehensive assessment of skin status and precise tailoring of cosmetological prescriptions. Biotype and phototype All patients presented dry–sensitive skin, predominantly phototypes II and III, which guided choice of textures and active ingredients. Personalized cosmetological prescriptions Each patient received recommendations for gentle cleansing, broad-spectrum sun protection, nighttime hydration with hyaluronic acid and peptides, and periodic soothing masks or superficial peels. All patients reported good tolerance and subjective improvement in skin comfort. In three patients with mild lymphedema, ultrasound identified structural changes despite the absence of clinical signs. This suggests a potential role for high-resolution ultrasound as a screening tool for detecting subclinical cases and guiding early rehabilitative intervention. Table 1. Clinical, dermo-aesthetic and ultrasound characteristics of the study population. This table summarizes the main demographic and clinical features of the 15 women included in the study, including lymphedema stage, skin parameters and ultrasound patterns of the dermo-epidermal complex and subcutis. 4. Discussion The results of this study confirm that post-mastectomy lymphedema is a complex condition with significant physical and emotional repercussions. The combination of clinical assessment, instrumental analysis, and dermocosmetologic check-up proved effective in understanding and addressing these issues. Measurements demonstrated impairment of the skin barrier, with dehydration, reduced superficial lipids, and increased TEWL. These findings indicate that simple moisturization is not sufficient: a structured plan is required, including gentle cleansing, targeted hydration, and daily photoprotection. The personalized cosmetological prescription was highly appreciated by patients; receiving recommendations grounded in objective data enhanced adherence and awareness, leading to perceived improvements not only in skin comfort but also in emotional well-being. This approach represents a bridge between aesthetic medicine and oncologic practice, paving the way for a closer collaboration between disciplines. The integration of ultrasound into the aesthetic check-up highlighted its value as a tool capable of detecting subclinical conditions and guiding the choice of dermo-aesthetic treatments. In early cases, fluid ultrasonographic patterns allowed clinicians to direct patients toward timely interventions such as manual lymphatic drainage, targeted physiotherapy, and cosmetological support, preventing progression to irreversible fibrotic stages. In cases with sclerotic or undifferentiated patterns, imaging provided an objective basis for calibrating aesthetic prescriptions, avoiding potentially ineffective treatments and suggesting tailored rehabilitative protocols (e.g. radiofrequency, fractional laser, biostimulatory fillers).This integrated check-up model is in line with international multidisciplinary recommendations (González et al., 2024), which emphasize the importance of personalizing interventions on the basis of clinical and instrumental criteria. The findings of this study are consistent with the literature regarding the frequency of lymphedema in patients treated for breast cancer. High-resolution ultrasound revealed alterations of the dermo-epidermal complex and subcutis in all cases, demonstrating high sensitivity in detecting structural changes. These findings are consistent with previous studies highlighting the value of ultrasound as a non-invasive and reproducible tool for the assessment of lymphedema. These findings suggest that ultrasound may support clinical stratification, allowing for a better allocation of therapeutic resources and personalization of rehabilitative and dermo-aesthetic interventions. 4.1 Strengths and Limitations of the Study The main strength of the study lies in the integration of clinical and ultrasound assessment, which enables a more accurate diagnosis and severity stratification based on objective parameters. However, the small sample size represents an important limitation that does not allow the results to be generalized. Furthermore, the observational and descriptive nature of the study does not permit definitive conclusions regarding the efficacy of specific therapeutic interventions. 5. Conclusions This work shows that a multidisciplinary approach combining clinical assessment, instrumental diagnostics, and personalized cosmetological prescription provides significant benefits for patients with post-mastectomy lymphedema. The integration of anamnesis, physical examination, and technological investigations enables the definition of targeted interventions, while cosmetology—when guided by objective data—becomes an integral component of therapy. Cosmetologic education promotes continuity of care and awareness of one’s own body. The study also confirms that high-resolution ultrasound, when incorporated into an aesthetic-medicine check-up, improves diagnostic capability and patient stratification in secondary lymphedema. Future Perspectives To consolidate the role of high-resolution ultrasound in the management of secondary lymphedema, multicenter studies with larger samples and inferential statistical analyses are needed. A further development could be the integration of ultrasound data with biomarkers and artificial intelligence tools to achieve more accurate early diagnosis. Finally, the combination of imaging and dermo-aesthetic protocols may help define new standards of care in oncologic rehabilitation. Abbreviations The following abbreviations are used in this manuscript: A.U. — Arbitrary Units BC — Breast Cancer BCRL — Breast Cancer–Related Lymphedema DEC — Dermo-Epidermal Complex F-DEC — Fluid Dermo-Epidermal Complex F-SUBC — Fluid Subcutis HRUS — High-Resolution Ultrasound ISL — International Society of Lymphology M-DEC — Mixed/Undifferentiated Dermo-Epidermal Complex MRI — Magnetic Resonance Imaging N-DEC — Normal Dermo-Epidermal Complex N-SUBC — Normal Subcutis PRP — Platelet-Rich Plasma S-DEC — Sclerotic Dermo-Epidermal Complex S-SUBC — Sclerotic Subcutis SPF — Sun Protection Factor SUBC — Subcutis TEWL — Transepidermal Water Loss US — Ultrasound Declarations Author Contributions: Conceptualization, A.M. and L.C.; methodology, A.M.; validation, A.M., L.C. and E.B.; formal analysis, A.M.; investigation, A.M.; resources, A.M.; data curation, A.M.; writing—original draft, A.M.; writing—review and editing, A.M. and L.C.; visualization, L.C.; supervision, E.B.; project administration, A.M. Funding: This research received no external funding. Institutional Review Board Statement: Ethical review and approval were waived for this study because all procedures were non-invasive, performed as part of routine clinical assessment, and involved anonymized data collected within standard care. The study was conducted in accordance with the Declaration of Helsinki. Informed Consent Statement: All patients signed informed consent. Data Availability Statement: Data supporting the findings of this study are not publicly available due to privacy restrictions. Anonymized data may be provided by the corresponding author upon reasonable request. Acknowledgments: The authors thank the clinical team and all patients who participated in the study.During the preparation of this manuscript, the authors used ChatGPT (OpenAI, GPT-5.1) exclusively for editing and language refinement; all content was reviewed and validated by the authors, who take full responsibility for the final text. Conflicts of Interest: The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. Disclaimer/Publisher’s Note The statements, opinions and data contained in this publication are solely those of the authors and do not necessarily reflect those of MDPI and/or the editors. MDPI and/or the editors disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content References Arman M, Rehnsfeldt A (2002) Living with Breast Cancer—A Challenge to Expansive and Creative Forces. Eur J Cancer Care 11:290–296. https://doi.org/10.1046/j.1365-2354.2002.00300.x DiSipio T, Rye S, Newman B, Hayes S (2013) Incidence of Unilateral Arm Lymphoedema after Breast Cancer: A Systematic Review and Meta-Analysis. 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Int J Radiat Oncol Biol Phys 81:907–914. https://doi.org/10.1016/j.ijrobp.2010.10.022 Shimizu Y, Ntege EH, Sunami H, Inoue Y (2022) Regenerative Medicine Strategies for Hair Growth and Regeneration: A Narrative Review. Regen Ther 21:527–539. https://doi.org/10.1016/j.reth.2022.10.005 Sussman TA, Kruse ML, Thacker HL, Abraham J (2019) Managing Genitourinary Syndrome of Menopause in Breast Cancer Survivors Receiving Endocrine Therapy. J Oncol Pract 15:363–370. https://doi.org/10.1200/JOP.18.00710 Youlden DR, Cramb SM, Dunn NAM, Muller JM, Pyke CM, Baade PD (2012) The Descriptive Epidemiology of Female Breast Cancer. Cancer Epidemiol 36:237–248. https://doi.org/10.1016/j.canep.2012.02.007 Table 1 Table 1 is available in the Supplementary Files section. Additional Declarations The authors declare no competing interests. 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15:46:02","extension":"png","order_by":16,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":31964,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage7.png","url":"https://assets-eu.researchsquare.com/files/rs-8290796/v1/2612f71238dc67ad54bc43e7.png"},{"id":97868648,"identity":"cb1a3c5b-89a8-4525-bae2-d124fd7e0a00","added_by":"auto","created_at":"2025-12-10 09:57:00","extension":"xml","order_by":17,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":103778,"visible":true,"origin":"","legend":"","description":"","filename":"rs82907960structuring.xml","url":"https://assets-eu.researchsquare.com/files/rs-8290796/v1/c50227baddd24e322e02a54e.xml"},{"id":97868646,"identity":"5e76f1bd-a354-42e0-95ad-b45440dd16fd","added_by":"auto","created_at":"2025-12-10 09:57:00","extension":"html","order_by":18,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":119676,"visible":true,"origin":"","legend":"","description":"","filename":"earlyproof.html","url":"https://assets-eu.researchsquare.com/files/rs-8290796/v1/ba095ff505723f805b1240cb.html"},{"id":97868622,"identity":"3fa0b668-2108-4d19-b811-8941f2731371","added_by":"auto","created_at":"2025-12-10 09:56:59","extension":"jpg","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":62757,"visible":true,"origin":"","legend":"\u003cp\u003ePitting sign\u003c/p\u003e","description":"","filename":"1.jpg","url":"https://assets-eu.researchsquare.com/files/rs-8290796/v1/ff76317cf932279c27ec9969.jpg"},{"id":97868623,"identity":"29950e41-ddd2-4649-a0bc-0947abc90f80","added_by":"auto","created_at":"2025-12-10 09:56:59","extension":"jpg","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":73468,"visible":true,"origin":"","legend":"\u003cp\u003eStemmer test a-b\u003c/p\u003e","description":"","filename":"2.jpg","url":"https://assets-eu.researchsquare.com/files/rs-8290796/v1/86e98af9b6a3db60cd73c205.jpg"},{"id":97900676,"identity":"f2c7b5d3-b78a-4132-915c-b29527ac0dbc","added_by":"auto","created_at":"2025-12-10 15:45:43","extension":"jpg","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":71605,"visible":true,"origin":"","legend":"\u003cp\u003eSchematic representation of the ultrasound image acquisition process\u003c/p\u003e","description":"","filename":"3.jpg","url":"https://assets-eu.researchsquare.com/files/rs-8290796/v1/c411f0d1786000ee3e13a6b6.jpg"},{"id":97868629,"identity":"f31b2ed7-2a81-42d0-b2ea-1cb295f66f55","added_by":"auto","created_at":"2025-12-10 09:57:00","extension":"jpg","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":119786,"visible":true,"origin":"","legend":"\u003cp\u003eUltrasound of superficial tissues in a healthy subject. The hyperechoic epidermis, the hypoechoic papillary dermis, and the hyperechoic reticular dermis can be identified, as well as the hypoechoic hypodermis traversed by hyperechoic fibrous septa.\u003c/p\u003e","description":"","filename":"4.jpg","url":"https://assets-eu.researchsquare.com/files/rs-8290796/v1/1c98fc569b52dae06ba2b42f.jpg"},{"id":97900091,"identity":"98e83190-2496-4b67-9d2b-53165e7c9d4d","added_by":"auto","created_at":"2025-12-10 15:45:13","extension":"jpg","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":66308,"visible":true,"origin":"","legend":"\u003cp\u003eTechnique for the correct execution of ultrasound examination of skin and subcutis.\u003c/p\u003e","description":"","filename":"5.jpg","url":"https://assets-eu.researchsquare.com/files/rs-8290796/v1/befb0b143a143a4de240b17d.jpg"},{"id":97899285,"identity":"b0522689-a045-4327-88c9-51397fa1760c","added_by":"auto","created_at":"2025-12-10 15:42:34","extension":"jpg","order_by":6,"title":"Figure 6","display":"","copyAsset":false,"role":"figure","size":50716,"visible":true,"origin":"","legend":"\u003cp\u003eDivision of the upper limb into 17 total sectors.\u003c/p\u003e","description":"","filename":"6.jpg","url":"https://assets-eu.researchsquare.com/files/rs-8290796/v1/fc9332eb6a0ee974114cb5ea.jpg"},{"id":97903409,"identity":"ff603d51-88c6-4460-9877-b0dbbd3cce24","added_by":"auto","created_at":"2025-12-10 15:55:22","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1537268,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-8290796/v1/aa1d2e35-0d22-4232-ab41-ee10925175b1.pdf"},{"id":97899776,"identity":"011d889f-e309-4a9e-b42b-83bc90d5d185","added_by":"auto","created_at":"2025-12-10 15:44:52","extension":"docx","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":215734,"visible":true,"origin":"","legend":"","description":"","filename":"Table1.docx","url":"https://assets-eu.researchsquare.com/files/rs-8290796/v1/88c25f77a6995806d9a3033d.docx"}],"financialInterests":"The authors declare no competing interests.","formattedTitle":"\u003cp\u003eUltrasound-Based Evaluation and Dermoaesthetic Recommendations for Secondary Lymphedema After Mastectomy for Breast Cancer\u003c/p\u003e","fulltext":[{"header":"Simple Summary","content":"\u003cp\u003eLymphedema of the arm is a frequent and debilitating consequence for women who undergo breast cancer surgery. It can cause swelling, discomfort, changes in the skin, and a strong impact on quality of life and body image. This study presents a new integrated check-up that combines clinical examination, instrumental skin measurements, and high-resolution ultrasound to identify early signs of lymphedema and guide personalized cosmetic recommendations. Ultrasound can reveal tissue changes even before visible swelling appears, allowing timely interventions that may prevent the condition from worsening. By analyzing skin hydration, lipids, pH, and water loss, the check-up also helps to create individualized skin-care programs aimed at improving comfort and supporting the skin barrier. This approach highlights how combining medical and dermo-aesthetic evaluation can provide more complete care for women after breast cancer treatment.\u003c/p\u003e"},{"header":"1. Introduction","content":"\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eGrowing attention to the quality of life of oncology patients has made aesthetic medicine a key supportive discipline in the post\u0026ndash;breast cancer rehabilitation pathway. Among the most frequent and disabling complications is secondary lymphedema, which, in addition to causing functional impairment, has a profound impact on body image and psychological well-being. Lymphedema is one of the most common and debilitating complications after surgical treatment of breast cancer. It presents as an accumulation of lymphatic fluid in the ipsilateral upper limb and may evolve\u0026mdash;if inadequately managed\u0026mdash;into skin thickening, fibrosis, and functional limitation. Beyond the clinical aspects, lymphedema deeply affects self-perception and psychological health. In recent years, focus has shifted from a purely therapeutic approach to a broader perspective that considers quality of life and body image. In this context, aesthetic medicine and cosmetology play a relevant role: the skin of a woman who has undergone breast surgery is not merely an outer \u0026ldquo;envelope\u0026rdquo; but a dynamic organ whose balance influences self-esteem and social relationships. International literature highlights that daily skin care, combined with targeted treatments, can improve comfort, prevent complications, and support psychophysical recovery. From this perspective emerges the idea of developing an integrated cosmetological check-up combining traditional medical evaluation with precision tools such as ultrasound and personalized cosmetological prescriptions. High-resolution ultrasound is now a reliable and reproducible tool for evaluating superficial tissues (dermo-epidermal complex and subcutis). When integrated into an aesthetic-medicine check-up, it allows for an early detection of clinical and subclinical changes, guiding the selection of personalized preventive and therapeutic strategies.The rationale of this study is therefore to propose an aesthetic check-up model integrated with ultrasound capable of providing an objective assessment and a scientific basis for subsequent aesthetic-medicine prescriptions, following international recommendations [\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e, \u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e, \u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e, \u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e, \u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e]. Secondary lymphedema in breast cancer is a direct consequence of impaired lymphatic drainage following oncologic surgical procedures, particularly sentinel lymph-node biopsy and, more commonly, complete axillary lymph-node dissection. The removal of these structures, with or without radiotherapy, disrupts the main lymphatic collectors of the arm, resulting in mechanical drainage insufficiency and subsequent accumulation of protein-rich interstitial fluid [\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e, \u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e].\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cdiv id=\"Sec2\" class=\"Section2\"\u003e\u003ch2\u003e1.1 Epidemiology of Breast Cancer and Definition of Lymphedema\u003c/h2\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eBreast cancer is the most common malignancy in women, affecting 1 in 8 women over a lifetime and accounting for approximately 29% of all female cancers. Incidence is highest in adult and older age groups, although an increasing trend is also observed among younger women. The disease is very rare in men, representing 1\u0026ndash;2% of all diagnoses, with incidence increasing with age. Advances in diagnostics and therapy have improved survival, yet long-term treatment-related complications significantly affect patients\u0026rsquo; quality of life [\u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e, \u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e]. Lymphedema is a chronic, progressive, and degenerative condition arising from partial impairment of lymphatic transport capacity. According to the International Society of Lymphology, it may involve skin, subcutaneous tissues, muscles, bones, nerves, joints, and internal organs, producing variable degrees of severity. Lymphedema is characterized by the accumulation of protein-rich interstitial fluid due to impaired lymphatic drainage. In oncology, secondary lymphedema is among the most common and disabling complications of breast cancer. Clinical classification is based on progressive stages (subclinical, reversible, irreversible, elephantiasis), while modern systems also consider tissue and fibrotic components, which are useful for guiding therapeutic decisions.\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cdiv id=\"Sec3\" class=\"Section3\"\u003e\u003ch2\u003e1.1.1 Clinical and Instrumental Diagnosis of Lymphedema\u003c/h2\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eDiagnosis relies on clinical criteria (inspection, palpation, limb-circumference measurement, water-displacement volumetry) and instrumental methods. Among these, lymphoscintigraphy and MRI enable assessment of lymphatic dynamics but are less practical in routine settings. High-resolution ultrasound, by contrast, provides direct visualization of the dermo-epidermal complex and subcutis, offering rapid, reproducible data that are easily integrated into clinical practice [\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e, \u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e, \u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e].\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\u003cdiv id=\"Sec4\" class=\"Section2\"\u003e\u003ch2\u003e1.2 Complications of Oncologic Treatments: Focus on Lymphedema\u003c/h2\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eAmong the most significant complications of surgical and radiotherapeutic treatments is secondary upper-limb lymphedema, a condition characterized by protein-rich fluid accumulation in the interstitial tissue with physical, functional, and aesthetic repercussions. Lymphedema may arise even years after undergoing an oncologic treatment and, if not recognized early, tends to become chronic, causing pain, functional limitations, and psychological distress. It is more accurate to refer to chronic edema (not to be confused with acute edema, a temporary post-traumatic fluid accumulation), defined as interstitial fluid accumulation lasting at least 3 months due to dynamic or mechanical lymphatic insufficiency. Dynamic (functional) lymphatic insufficiency refers to conditions where fluid accumulation results from increased capillary filtration despite a structurally intact lymphatic system. Causes include venous insufficiency, inflammatory processes, and conditions altering oncotic pressure (e.g., hypoalbuminemia). Dynamic insufficiency may evolve into mechanical insufficiency. Mechanical lymphatic insufficiency refers to structural damage\u0026mdash;primary or acquired\u0026mdash;of the lymphatic system. Beyond a critical threshold of capillary filtrate, intrinsic lymphatic dysfunction prevents adequate reabsorption. Persistent interstitial stagnation triggers mechanotransduction processes stimulating fibroblasts to produce collagen fibers, leading to tissue sclerosis and preadipocyte proliferation.\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cdiv id=\"Sec5\" class=\"Section3\"\u003e\u003ch2\u003e1.2.1 Classification\u003c/h2\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eSeveral classification systems exist, each emphasizing different aspects. When the etiology is considered, lymphedema may be classified as primary, secondary, or mixed. From a pathogenetic perspective, it may be classified as functional/overload (dynamic insufficiency) or mechanical insufficiency.\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec6\" class=\"Section3\"\u003e\u003ch2\u003e1.2.2 Staging\u003c/h2\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eMultiple staging systems have been proposed, though none has achieved universal consensus, as no single system is considered fully comprehensive. The most widely used is the International Society of Lymphology staging system, which divides lymphedema into four stages based on clinical and instrumental criteria:\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003cul\u003e\u003cli\u003e\u003cp\u003eStage 0 / Ia: Subclinical stage in which edema is not clinically detectable, although patients may report suggestive symptoms and instrumental findings may be present. This stage may persist for months or years before becoming clinically evident.\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eStage I / Ib: Edema is visible and may regress during the day or with limb elevation; pitting may be present.\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eStage II: Persistent, non-reversible edema with pitting; tissue changes and fibrosis modify skin consistency.\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eStage III: The limb is markedly deformed by lymphostasis, with non-pitting edema due to advanced tissue fibrosis; cutaneous alterations may also be present.\u003c/p\u003e\u003c/li\u003e\u003c/ul\u003e\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec7\" class=\"Section3\"\u003e\u003ch2\u003e1.2.3 Diagnosis\u003c/h2\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eDiagnostic work-up should include detailed medical history, careful physical examination, and instrumental support. Risk factors such as previous malignancies, adjuvant or neoadjuvant radio-chemotherapy, and potential exposure to infectious agents (e.g., tropical travel) must be investigated. A positive family history may suggest hereditary forms, while associated signs and symptoms can indicate syndromic presentations. Patients with lymphedema report symptoms varying with severity, including heaviness, swelling, tightness, discomfort, and functional limitations. In severe cases, skin discoloration, thickening, and pruritus may also occur. A thorough clinical evaluation involves the assessment of morphological contours: reduced visibility of extensor tendons, superficial venous network, and bony prominences (e.g., knuckles, styloid processes in the upper limb). Surgical scars must be examined, as dystrophic or adherent scars may impede lymphatic return. Factors such as radiotherapy, aromatase inhibitors, surgical technique, weight gain, and age can affect one\u0026rsquo;s range of motion, particularly at the shoulder. In advanced stages, cutaneous signs of fibrosis may develop, including hyperkeratosis, lymphatic cysts, lichenification, and accentuation of the dermal papillae (peau d\u0026rsquo;orange). Potential complications should also be carefully assessed.\u003c/p\u003e\u003cp\u003eOn palpation, characteristic findings include:\u003c/p\u003e\u003cp\u003e\u0026bull; Consistency:\u003c/p\u003e\u003cp\u003eTexture varies with severity. Early stages show soft tissue due to fluid accumulation. With fibrosis affecting skin and subcutis, tissue becomes firm or woody. A grading scale may be used:\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003cul\u003e\u003cli\u003e\u003cp\u003eGrade 0: normal\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eGrade 1: elastic\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eGrade 2: fibro-elastic\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eGrade 3: fibrotic\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003ePitting Edema Scale:\u003c/p\u003e\u003c/li\u003e\u003c/ul\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eAssessed by maintaining pressure for at least 10 seconds and evaluating the depth and persistence of the indentation:\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003cul\u003e\u003cli\u003e\u003cp\u003eGrade 1: \u0026lt;2 mm, immediate rebound\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eGrade 2: 2\u0026ndash;4 mm, disappears within seconds\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eGrade 3: 4\u0026ndash;6 mm, disappears within 10\u0026ndash;12 seconds\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eGrade 4: 6\u0026ndash;8 mm, persists\u0026thinsp;\u0026gt;\u0026thinsp;20 seconds\u003c/p\u003e\u003c/li\u003e\u003c/ul\u003e\u003c/p\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eSkin Foldability: also known as the Stemmer test, it consists of assessing the increase in cutaneous foldability\u0026mdash;normally difficult to detect\u0026mdash;at the dorsal aspect of the proximal phalanx of the second toe or finger. In the early stages of lymphedema, foldability appears increased due to the presence of fluid layers within the dermis and subcutis; in more advanced stages, however, fibrotic degeneration leads to reduced foldability. In some cases, the assessment of foldability may be extended to the entire limb\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eVolumetric measurements: that is, the assessment\u0026mdash;using a measuring tape\u0026mdash;of any increase in the size of the body region affected by lymphedema. The assessment involves multiple anatomical reference points. For the upper limb, the first measurement is performed at the level of the hand, using the figure-of-eight measurement technique.\u003c/p\u003e\u003cp\u003eThe second measurement is taken at the level of the distal wrist crease, used as a reference point because it tends not to disappear even in the most severe cases of obesity. From this landmark, subsequent measurements are taken every 5 cm in a distal-to-proximal direction. According to the new guidelines, however, other parameters are now preferred. Regarding simple circumferential measurements, an increase of at least 2 cm compared with the healthy contralateral limb at any single reference point is considered indicative of lymphedema. These measurements also make it possible to compare the percentage volumetric variations\u0026mdash;again relative to the healthy contralateral side. Instrumental examinations: Although medical history and physical examination are generally sufficient for the diagnosis of lymphedema, integrating first- or second-level instrumental assessments is often valuable\u0026mdash;particularly to clarify ambiguous cases, better define disease severity, and differentiate among potential etiologies.\u003c/p\u003e\u003cp\u003eThe most commonly used instrumental examinations include:\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003cul\u003e\u003cli\u003e\u003cp\u003eHigh-resolution ultrasound\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eDoppler ultrasound: useful for evaluating the deep venous system\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eLymphoscintigraphy: useful for assessing superficial and deep lymphatic vessels and lymph nodes and therefore considered the gold standard for diagnosing lymphatic abnormalities\u003c/p\u003e\u003c/li\u003e\u003c/ul\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eFor the diagnosis of lymphedema, at least one clinical criterion and one suggestive lymphoscintigraphic finding are required. This technique involves the injection of a radiotracer into the subcutaneous tissue at the first interdigital space of both hands or feet, depending on the location of lymphedema; the radiopharmaceutical is typically administered in two low-dose injections (thus carrying very low risk of ionizing radiation exposure and allergies). As it follows the lymphatic pathways, it reveals outflow obstructions through gamma-camera detection. The main limitation of this technique is insufficient standardization, as protocols differ regarding the radiotracer dose, injection site, and physical activity required during the exam. For preventive purposes, lymphoscintigraphy is recommended for patients at high risk of subclinical secondary lymphedema (e.g., after major lymphadenectomy at the limb root) and for relatives of patients with primary lymphedema.\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003cul\u003e\u003cli\u003e\u003cp\u003eLymphography: usually performed with direct oily contrast; rarely used today except in specific situations such as congenital or acquired disorders of the chylous vessels, cisterna chyli, or thoracic duct\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eBioimpedance analysis: a technique that correlates variations in electrical conduction velocity with tissue composition; it enables the detection of subclinical lymphedema by identifying even minimal compositional changes in the limb. Recent studies suggest that bioimpedance analysis, when normalized to limb length, may provide a standardized outpatient protocol for the quantitative assessment of unilateral lymphedema, offering greater reliability than simple circumferential measurements.\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003ePerometry: a technique that uses infrared light beams to assess limb volume; it is extremely rapid, accurate, and reproducible, and is primarily used in specialized centers\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eMR lymphangiography: particularly when using fat-suppression digital subtraction techniques, it can provide important information in advanced obstructive cases where lymphatic pathways appear dilated and filled with lymph\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eCT lymphangiography: also used in complex cases involving vessel or cisterna chyli abnormalities\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eWater-displacement plethysmography: considered the gold standard for calculating limb volume and the only reliable method for measuring edematous hands and feet. The principle is that a submerged body displaces a volume of water equal to its own. However, practical limitations\u0026mdash;such as hygiene concerns \u0026mdash; restrict its use\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eFilarial antigen card test: used to detect Wuchereria bancrofti infection (Bancroftian filariasis), and therefore a possible infectious etiology of lymphedema, by identifying antibodies in individuals who live in or have traveled to endemic areas\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eGenetic testing: identifies the most frequent mutations associated with familial primary lymphedema (e.g., FOXC2, VEGFR-3, SOX18).\u003c/p\u003e\u003c/li\u003e\u003c/ul\u003e\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\u003cdiv id=\"Sec8\" class=\"Section2\"\u003e\u003ch2\u003e1.3 Aesthetic and Functional Impact of Oncologic Treatments\u003c/h2\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eOncologic therapies (surgery, radiotherapy, chemotherapy, hormone therapy) induce cutaneous, scar-related, musculoskeletal, and metabolic alterations. Patients may experience skin dryness, rash, alopecia, nail changes, fibrosis, and lymphedema\u0026mdash;all of which, in addition to impairing function, can profoundly affect body image and self-esteem. For this reason, aesthetic and functional recovery is an integral component of oncologic rehabilitation (Arman \u0026amp; Rehnsfeldt, 2002).\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec9\" class=\"Section2\"\u003e\u003ch2\u003e1.4 Multidisciplinary Approach: Oncology, Physiotherapy, Dermatology, Aesthetic Medicine\u003c/h2\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eRecent literature emphasizes the value of a multidisciplinary approach integrating oncology, dermatology, physiotherapy, nutrition, reconstructive plastic surgery (aimed at restoring anatomical and functional integrity), and aesthetic medicine, which focuses on minimally invasive and dermo-aesthetic treatments to improve skin quality and body image. International recommendations propose safe, personalized dermo-aesthetic interventions to improve the quality of life of breast cancer patients. These include the management of cutaneous toxicities [\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e, \u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e], physiotherapy for lymphedema prevention and treatment, reconstructive techniques, and minimally invasive aesthetic therapies\u0026mdash;all in close collaboration with the referring oncologist.\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec10\" class=\"Section2\"\u003e\u003ch2\u003e1.5 Basics of Ultrasound\u003c/h2\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eUltrasound imaging is a highly versatile and easy-to-use diagnostic technique that employs mechanical waves\u0026mdash;ultrasound\u0026mdash;to generate digital images. Diagnostic ultrasound typically uses frequencies ranging from 2\u0026ndash;3 MHz to 20 MHz. These waves propagate through tissues via cycles of compression and rarefaction at speeds determined by the physical properties of the medium. They are generated by the transducer, a component of the ultrasound machine that contains a piezoelectric material, which changes shape in response to an applied electrical potential.\u003c/p\u003e\u003cp\u003eWhen ultrasound waves encounter tissues with different impedance\u0026mdash;a physical property related to tissue density\u0026mdash;a portion of the waves is reflected and captured by the ultrasound system. In other words, impedance variations, known as interfaces, are detected, and converted into an analog signal, with depth represented on the x-axis and signal amplitude (in dB) on the y-axis. The analog trace is then converted into a digital signal in which each characteristic corresponds to white, black, or a shade of gray in the final image.\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cdiv id=\"Sec11\" class=\"Section3\"\u003e\u003ch2\u003e1.5.1 Ultrasound of Healthy Superficial Tissues\u003c/h2\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eTypically, probes used for the assessment of the skin and subcutis operate at a frequency of 20 MHz, although an accurate ultrasound examination can be performed with any probe of at least 15 MHz. Frequencies higher than 20 MHz, despite offering potentially greater detail, allow for reduced tissue penetration. Standard ultrasound systems are used, without requiring dedicated software.\u003c/p\u003e\u003cp\u003eUltrasound imaging of superficial tissues allows for the visualization of:\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003cul\u003e\u003cli\u003e\u003cp\u003eEpidermis, the outermost layer, which appears as a thin, uniform hyperechoic line.\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eDermis, located deeper, which appears as a hyperechoic band (although less hyperechoic than the epidermis), with thickness varying according to the anatomical region. The dermis comprises a more superficial papillary dermis, which is more hypoechoic due to its high water content, and a deeper reticular dermis, which is more hyperechoic because of the density and orientation of collagen fibers. The epidermis, papillary dermis, and reticular dermis together form the dermo-epidermal complex (DEC).\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eHypodermis/subcutis (SUBC), the innermost layer, clearly separated from the DEC by the dermo-hypodermal junction, a continuous, well-defined line reflecting the structural differences between the dermis and hypodermis. The hypodermis appears hypoechoic, organized into lobules, and intersected by thin horizontal or oblique hyperechoic lines representing connective-tissue septa (scaffolds) through which lymphatic vessels run. These septa tend to be parallel to the muscular fascia and may form interconnections with it. The end of this layer is marked by the beginning of the muscular layer, and its thickness varies according to anatomical site and adipose-tissue content.\u003c/p\u003e\u003c/li\u003e\u003c/ul\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eA proper evaluation of superficial tissues should also include the generous application of gel and the use of minimal pressure during probe manipulation. These precautions help prevent excessive deformation of the underlying tissues, allowing for an accurate visualization of structures without operator-induced artifacts.\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec12\" class=\"Section3\"\u003e\u003ch2\u003e1.5.3 Ultrasound of Superficial Tissues in Patients with Lymphedema\u003c/h2\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eThe rationale for the ultrasound assessment of superficial tissues in patients with lymphedema does not lie in identifying intrinsic damage to the lymphatic system itself, as the resolution of standard ultrasound equipment used for this type of examination does not allow direct visualization of lymphatic vessels. Specifically, when affected by disease, the deeper canalicular component containing lymphatic collectors may become visible due to dilation and disruption of the fibrous scaffold; however, the subepidermal lymphatic plexus cannot be directly evaluated.\u003c/p\u003e\u003cp\u003eThe objective, therefore, is to identify indirect tissue alterations that reflect lymphatic dysfunction, such as differences in echogenicity and thickness of the various layers of the skin and subcutis. In unilateral lymphedema, the healthy contralateral limb is always used as a point of comparison; in bilateral cases, previous examinations serve as the reference point.\u003c/p\u003e\u003cp\u003eAlthough the number of studies evaluating the role of ultrasound in lymphedema is still limited, several have laid the groundwork for its systematic use in clinical assessment. Of particular relevance is the study by Tassenoy et al. (2011), an observational study involving seven women with unilateral breast cancer who developed lymphedema. Limb volume increase was measured using water-displacement plethysmography, and ultrasound findings were correlated with MRI to assess tissue-structure changes [\u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e].\u003c/p\u003e\u003cp\u003eSimilarly, the retrospective study by Suehiro et al. (2013) examined 35 patients with secondary lower-limb lymphedema by evaluating the skin and subcutis at eight standardized points. In the subcutaneous tissue, three grades of echogenicity were identified. This study highlighted that variations in tissue echogenicity correlate with lymphedema stage according to the International Society of Lymphology, and that such evaluation is feasible using probes operating at 11 MHz [\u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e21\u003c/span\u003e].\u003c/p\u003e\u003cp\u003eOne of the most influential studies demonstrating the feasibility of a standardized ultrasound assessment protocol in lymphedema was conducted by Mander et al. (2019). This study precisely identified distinct ultrasonographic patterns representing progressive histological degeneration in the dermo-epidermal complex (DEC) and subcutis (SUBC) of the upper limb after mastectomy. These findings laid the foundation for a more objective evaluation of lymphatic-system compromise [20,21,22].\u003c/p\u003e\u003cp\u003eBased on Mander\u0026rsquo;s work, characteristic ultrasound patterns can be identified in both the dermo-epidermal complex and the subcutis, each reflecting progressive stages of histological alteration. Specifically, for the dermo-epidermal complex, the following patterns may be observed:\u003c/p\u003e\u003cp\u003e\u0026bull; Dermal edema\u003c/p\u003e\u003cp\u003eThis condition is characterized by increased DEC thickness and reduced echogenicity (hypoechoic dermis). It is caused by the dilation of the subepidermal lymphatic plexus, leading to leakage of fluid into the dermal interstitium and the formation of vessel-like structures between collagen bundles lacking endothelial lining (a phenomenon known as dermal backflow). The papillary dermis is typically affected earlier than the reticular dermis due to differences in vascular and lymphatic distribution and collagen architecture.\u003c/p\u003e\u003cp\u003eSome authors suggest an association between dermal edema and paresthesias, attributed to stretching and irritation of sensory nerve endings.\u003c/p\u003e\u003cp\u003eThis acute-stage pattern is sonographically identified as F-DEC (fluid dermo-epidermal complex).\u003c/p\u003e\u003cp\u003e\u0026bull; Dermal sclerosis with infiltrative changes\u003c/p\u003e\u003cp\u003eThis pattern shows a marked increase in DEC thickness, increased echogenicity (hyperechoic dermis), and loss of the typical trilaminar DEC structure. These findings are due to cellular infiltration and early collagen-fiber deposition.\u003c/p\u003e\u003cp\u003eThis pattern reflects a subacute or evolving stage and is identified as S-DEC (sclerotic dermo-epidermal complex). It is considered partially reversible.\u003c/p\u003e\u003cp\u003e\u0026bull; Dermal sclerosis with fibrotic involution\u003c/p\u003e\u003cp\u003eThis condition is characterized by the loss of the dermo-hypodermal interface, resulting in a homogeneous echogenic appearance across tissues, making it difficult or impossible to accurately measure individual layers. These changes are due to chronic collagen deposition, ultimately leading to dermal fibrosis.\u003c/p\u003e\u003cp\u003eThis chronic, irreversible stage is identified as M-DEC (mixed or markedly sclerotic dermo-epidermal complex).\u003c/p\u003e\u003cp\u003eThe normal pattern of the dermo-epidermal complex is labeled N-DEC.\u003c/p\u003e\u003cp\u003eRegarding the subcutaneous tissue, the following patterns may be observed:\u003c/p\u003e\u003cp\u003e\u0026bull; Dilation of lymphatic ducts\u003c/p\u003e\u003cp\u003eIn this condition, the hyperechoic signal of the fibrous connective septa disappears and the subcutis becomes globally thickened. This disappearance is due to the dilation of the lymphatic collectors running within the fibrous strands, which leads to a marked separation of the adipose lobules (a cobblestone appearance). In some cases, dilated lymphatic collectors may cause a rupture of the fibrous septa, resulting in the formation of so-called lymph lakes. Unlike dilated lymphatic collectors, lymph lakes collapse under pressure from the ultrasound probe.\u003c/p\u003e\u003cp\u003eThis pattern is identified as F-SUBC (fluid subcutaneous).\u003c/p\u003e\u003cp\u003e\u0026bull; Edema and infiltrative phenomena of adipose lobules\u003c/p\u003e\u003cp\u003eThis condition is characterized by increased echogenicity of the subcutaneous tissue, poor visualization of the fibrous framework, and overall thickening of the subcutis. It is caused by an infiltration of fluid and cells into the stromal component of adipose lobules\u0026mdash;i.e., at the intercellular level\u0026mdash; resulting in a sclerotic-edematous appearance known as \u0026ldquo;snow-fall\u0026rdquo;. In addition, mechanical pressure within the lobules may lead to the formation of a network of microscopic fissures in which fluid can accumulate.\u003c/p\u003e\u003cp\u003e\u0026bull; Subcutaneous sclerosis with fibrotic involution\u003c/p\u003e\u003cp\u003eThis condition shows increased echogenicity and reduced thickness of the subcutis. It results from the replacement of adipose lobules with fibrotic tissue and collagen fibers, producing a fibro-sclerotic pattern. When the subcutis reaches this stage, and when a dermo-epidermal complex already shows dermal fibrosis, the dermo-hypodermal interface may become indistinguishable.\u003c/p\u003e\u003cp\u003eThese latter two patterns are classified as S-SUBC (sclerotic subcutaneous).\u003c/p\u003e\u003cp\u003eThe normal pattern of the subcutis is labeled N-SUBC.\u003c/p\u003e\u003cp\u003eIn clinical practice, differentiation between patterns is not always clear-cut, and features of different patterns may coexist within the same ultrasound image, producing blended or transitional appearances.\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec13\" class=\"Section3\"\u003e\u003ch2\u003e1.5.4 Ultrasound Examination Protocol\u003c/h2\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eAs noted previously, the ultrasound evaluation was performed several weeks after the outpatient visit, within the National Health Service framework for patients with suspected chronic secondary lymphedema after mastectomy for breast cancer. The examination\u0026mdash;always conducted by the same operator\u0026mdash;required several technical precautions, such as the use of abundant gel, positioning the probe perpendicular to the skin, and stabilizing the grip by resting the little finger on the patient\u0026rsquo;s skin.\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eThe standardized approach to the ultrasound examination, as outlined by Mander et al. and later by Ricci et al., involved dividing the upper limb into 17 total sectors: one sector on the dorsal surface of the hand near the third metacarpal ray, four anterior and four posterior sectors below the elbow, and four anterior and four posterior sectors above the elbow.\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eEach sector was then analyzed both qualitatively and quantitatively.\u003c/p\u003e\u003cp\u003eFrom a qualitative standpoint, the patterns previously described by Mander et al. and later by Ricci et al. were used to classify the findings. Specifically, for the dermo-epidermal complex (DEC):\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003cul\u003e\u003cli\u003e\u003cp\u003eN-DEC: normal dermo-epidermal complex\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eF-DEC: fluid dermo-epidermal complex\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eS-DEC: sclerotic dermo-epidermal complex\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eM-DEC: undifferentiated dermo-epidermal complex\u003c/p\u003e\u003c/li\u003e\u003c/ul\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eFor the subcutis (SUBC):\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003cul\u003e\u003cli\u003e\u003cp\u003eN-SUBC: normal subcutis\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eF-SUBC: fluid subcutis\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eS-SUBC: sclerotic subcutis\u003c/p\u003e\u003c/li\u003e\u003c/ul\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eFrom a quantitative standpoint, it was possible to measure dermal and subcutaneous thickness and compare these values with those of the healthy contralateral limb. Dermal thickness was measured from the deep surface of the epidermis to the dermis\u0026ndash;subcutis transition; subcutaneous thickness was measured from the dermis\u0026ndash;subcutis junction to the deep fascia.\u003c/p\u003e\u003cp\u003e(Tassenoy et al., 2011; Suehiro et al., 2013; Mander et al., 2019; Ricci et al., 2021)\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec14\" class=\"Section3\"\u003e\u003ch2\u003e1.5.5 International Guidelines and Dermo-Aesthetic Recommendations\u003c/h2\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eRecent multidisciplinary recommendations highlight the need for an integrated approach to the management of patients with breast cancer. In particular, the international article by Gonz\u0026aacute;lez et al. (2024) provides clinical guidelines for aesthetic medicine and oncologic rehabilitation.\u003c/p\u003e\u003cp\u003eThese interventions must be incorporated into a multidisciplinary pathway involving oncologists, dermatologists, physiotherapists, nutritionists, and specialists in aesthetic and reconstructive medicine.\u003c/p\u003e\u003cp\u003eThey include:\u003c/p\u003e\u003cp\u003eDermatology: Prevention and management of cutaneous toxicities using emollients, photoprotection, and targeted treatments for alopecia, hand\u0026ndash;foot syndrome, and nail alterations.\u003c/p\u003e\u003cp\u003ePhysiotherapy: Manual lymphatic drainage, progressive exercise, prevention and treatment of lymphedema, mobilization techniques, and hydrotherapy.\u003c/p\u003e\u003cp\u003ePlastic Surgery: Breast reconstruction procedures.\u003c/p\u003e\u003cp\u003eAesthetic Medicine: Scar treatments, fillers and botulinum toxin, laser, and radiofrequency devices, with specific recommendations regarding timing.\u003c/p\u003e\u003cp\u003eNutrition: Mediterranean diet and body-weight management as prognostic factors.\u003c/p\u003e\u003cp\u003eDentistry: Prevention and management of oral complications (mucositis, xerostomia, bisphosphonate-related osteonecrosis of the jaw).\u003c/p\u003e\u003cp\u003eGynecology and Aesthetic Gynecology: Management of genitourinary syndrome of menopause (GSM) with non-hormonal or low-risk topical options.\u003c/p\u003e\u003cp\u003eThe integration of these recommendations into clinical practice improves quality of life and reduces the side effects associated with cancer treatments.\u003c/p\u003e\u003cp\u003eDermo-Aesthetic Interventions\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\n\u003ch3\u003e1. Scar Treatments\u003c/h3\u003e\n\u003cp\u003e\u003cul\u003e\u003cli\u003e\u003cp\u003eFractional lasers (CO₂, Er:YAG) and radiofrequency: improve skin texture and post-radiotherapy fibrosis\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eMicroneedling: stimulates dermal regeneration, useful for linear scars\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eBotulinum toxin: reduces scar retraction and scar-related pain\u003c/p\u003e\u003c/li\u003e\u003c/ul\u003e\u003c/p\u003e\n\u003ch3\u003e2. Regenerative Treatments\u003c/h3\u003e\n\u003cp\u003e\u003cul\u003e\u003cli\u003e\u003cp\u003ePRP: stimulates fibroblasts and angiogenesis through growth factors\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eLipofilling: restores volume and improves skin trophism\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eNanofat: enhances skin texture and radiotherapy-induced dyschromias\u003c/p\u003e\u003c/li\u003e\u003c/ul\u003e\u003c/p\u003e\n\u003ch3\u003e3. Fillers and Botulinum Toxin\u003c/h3\u003e\n\u003cp\u003e\u003cul\u003e\u003cli\u003e\u003cp\u003eHyaluronic acid fillers: correct scar depressions and contour irregularities\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eCollagen stimulators: induce progressive improvement in skin quality\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eBotulinum toxin: also beneficial for post-mastectomy neuropathic pain\u003c/p\u003e\u003c/li\u003e\u003c/ul\u003e\u003c/p\u003e\n\u003ch3\u003e4. Laser and Energy-Based Devices\u003c/h3\u003e\n\u003cp\u003e\u003cul\u003e\u003cli\u003e\u003cp\u003eCO₂/Er:YAG lasers: surgical scars, fibrosis, radiotherapy tattoos\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eIPL: treatment of skin dyschromias\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eCarboxytherapy: improves microcirculation and reduces scar fibrosis\u003c/p\u003e\u003c/li\u003e\u003c/ul\u003e\u003c/p\u003e\n\u003ch3\u003e5. Reconstructive Plastic Surgery\u003c/h3\u003e\n\u003cp\u003e\u003cul\u003e\u003cli\u003e\u003cp\u003eDIEP flap and autologous flaps for breast reconstruction\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eImplants with or without tissue expanders\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eMastopexy or mastoplasty for post-treatment asymmetries\u003c/p\u003e\u003c/li\u003e\u003c/ul\u003e\u003c/p\u003e\n\u003ch3\u003e6. General Recommendations\u003c/h3\u003e\n\u003cp\u003e\u003cul\u003e\u003cli\u003e\u003cp\u003eTiming: preferably 6\u0026ndash;12 months after oncologic treatments\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003ePersonalization: procedures should be calibrated based on clinical and ultrasound findings\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eMultidisciplinarity: coordination among oncologists, plastic surgeons, dermatologists, and aesthetic physicians\u003c/p\u003e\u003c/li\u003e\u003c/ul\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eBreast cancer patients frequently experience cutaneous and physical side effects resulting from oncologic treatments. For this reason, dermo-aesthetic care plays an important role in improving quality of life, body image, and overall well-being[\u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e, \u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e, \u003cspan citationid=\"CR18\" class=\"CitationRef\"\u003e18\u003c/span\u003e]. Proper skin care for the face and body is essential, as treatments can lead to dryness, pruritus, and irritation. The use of gentle cleansers, emollients, fragrance-free creams, and consistent photoprotection, helps to maintain the integrity of the skin barrier. During treatment, the scalp and hair become fragile: scalp cooling can reduce the risk of alopecia by half, while wigs and headscarves provide valuable psychological and aesthetic support. Hand\u0026ndash;foot syndrome may cause pain, swelling, and erythema, affecting daily activities; therefore, preventive and soothing treatments are recommended. Nail changes\u0026mdash;including discoloration, fragility, and periungual infections\u0026mdash;also require preventive and targeted management. All procedures must be carefully evaluated and coordinated with the oncologist to ensure safety and individualized care. In summary, dermo-aesthetic recommendations include tailored skincare, strategies against alopecia and nail fragility, and aesthetic and reconstructive support, always within a multidisciplinary framework involving oncology, dermatology, and aesthetic medicine.\u003c/p\u003e\u003cp\u003eThe goal is to reduce treatment-related side effects, improve self-perception, and restore overall well-being. Aesthetic medicine procedures therefore represent fundamental rehabilitative tools for patients treated for breast cancer. When properly integrated, they improve quality of life, self-esteem, and adherence to therapeutic programs. The integration of high-resolution ultrasound further allows for the personalization of treatments based on clinical stage.\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\n\u003ch3\u003e2 – Study Objectives\u003c/h3\u003e\n\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eThe primary objective of this study is to propose an integrated aesthetic check-up model incorporating ultrasound imaging, capable of providing an objective evaluation and a scientific basis for the subsequent aesthetic-medicine prescription, in accordance with international recommendations [\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e].\u003c/p\u003e\u003cp\u003eThe study aims to:\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003cul\u003e\u003cli\u003e\u003cp\u003eCorrelate ultrasound findings with the clinical severity of lymphedema;\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eIdentify subclinical presentations at an early stage, improving the timeliness of therapeutic intervention;\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003ePerform personalized cosmetological prescriptions to enhance skin well-being, support the hydrolipidic barrier, and contribute to improved self-perception;\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eCompare the data obtained with international dermo-aesthetic recommendations.\u003c/p\u003e\u003c/li\u003e\u003c/ul\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eThis approach stems from the awareness that lymphedema is not merely a functional disorder; it also entails significant aesthetic and psychological consequences, requiring comprehensive management. Integrating medical, instrumental, and cosmetological assessments therefore allows a more complete response to the needs of women treated for breast cancer.\u003c/p\u003e\u003cp\u003eThe study enrolled 15 patients, each undergoing a standardized, multi-step pathway consisting of: general and cosmetological anamnesis, clinical and palpatory examination, instrumental analysis (corneometry, sebometry, pH measurement, TEWL, high-resolution ultrasound), biotype and phototype classification, and development of a personalized cosmetological prescription. All cases safeguard patient privacy and adhere to ethical research principles.\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e"},{"header":"2. Materials and Methods","content":"\u003cdiv id=\"Sec23\" class=\"Section2\"\u003e\u003ch2\u003e2.1 Study Design\u003c/h2\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eThis observational study focuses on the ultrasound evaluation of secondary upper-limb lymphedema in patients treated for breast cancer. The analysis was conducted using a standardized approach to ensure data reproducibility.\u003c/p\u003e\u003cp\u003eAll patients underwent an integrated aesthetic check-up protocol, which included:\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003cul\u003e\u003cli\u003e\u003cp\u003eGeneral and dermatologic clinical assessment\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eLimb volumetric measurements\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eHigh-resolution ultrasound of the dermo-epidermal complex and subcutis\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eCollection of medical and aesthetic history\u003c/p\u003e\u003c/li\u003e\u003c/ul\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eThe results of the integrated check-up were then compared with international dermo-aesthetic recommendations, to define the most suitable prescription for each clinical profile.The diagnosis of lymphedema requires a careful examination combining inspection, palpation, and objective measurements. Inspection allows for the assessment of limb symmetry, localized edema, chromatic alterations, and signs of fibrosis. Palpation differentiates soft edema, characterized by evident pitting, from fibrotic edema. Pitting is assessed by applying digital pressure for a few seconds and observing whether an indentation remains, as described in the introductory section. A positive Stemmer sign\u0026mdash;when the skin at the base of the second toe or finger cannot be lifted\u0026mdash;indicates advanced lymphedema.To quantify swelling, circumferential measurements at different limb levels can be compared with the contralateral side, or water volumetry can be performed for a direct volume estimate.\u003c/p\u003e\u003cp\u003eClassification follows the criteria of the International Society of Lymphology (ISL):\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003cul\u003e\u003cli\u003e\u003cp\u003eStage 0 (latent) \u0026ndash; no visible edema but demonstrable lymphatic impairment\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eStage I \u0026ndash; edema reversible with rest\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eStage II \u0026ndash; partially irreversible edema with initial fibrosis\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eStage III \u0026ndash; chronic lymphedema with marked fibrosis and skin alterations\u003c/p\u003e\u003c/li\u003e\u003c/ul\u003e\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec24\" class=\"Section2\"\u003e\u003ch2\u003e2.2 Study Population\u003c/h2\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003e Fifteen patients with clinically confirmed unilateral secondary upper-limb lymphedema were enrolled, all of whom had signed an informed consent.\u003c/p\u003e\u003cp\u003eInclusion criteria: prior breast cancer treated with surgery and/or radiotherapy; clinical signs of lymphedema; willingness to participate.\u003c/p\u003e\u003cp\u003eExclusion criteria: no oncologic history; primary lymphedema; contraindications to ultrasound examination.\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec25\" class=\"Section2\"\u003e\u003ch2\u003e2.3 Ultrasound Protocol\u003c/h2\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eUltrasound evaluation was performed using an Esaote system configured for \u0026lsquo;superficial musculoskeletal\u0026rsquo; imaging. The standardized approach divided the upper limb into sectors: 4 anterior and 4 posterior for the arm, and 4 anterior and 4 posterior for the forearm. Landmark points were defined by tracing two perpendicular lines intersecting at the midpoint of the arm and forearm. The examination included an assessment of the dermo-epidermal complex and subcutaneous tissue. Ultrasound has become a key tool in lymphedema assessment, providing real-time visualization of the skin and subcutaneous tissues without exposure to radiation. The different regions\u0026mdash;axilla, proximal and distal arm, forearm, and dorsum of the hand\u0026mdash;were systematically analyzed for dermal thickness, adipose-tissue echogenicity, and signs of fibrosis. This ultrasound classification enables longitudinal monitoring of lymphedema progression and evaluation of treatment efficacy.\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec26\" class=\"Section2\"\u003e\u003ch2\u003e2.4 Parameters Assessed\u003c/h2\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eThe following ultrasound patterns were identified and classified:\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003cul\u003e\u003cli\u003e\u003cp\u003eDermo-epidermis: normal, fluid, sclerotic, undifferentiated\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eSubcutis: normal, fluid, sclerotic\u003c/p\u003e\u003c/li\u003e\u003c/ul\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eThese parameters were correlated with clinical assessment of lymphedema severity.\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec27\" class=\"Section2\"\u003e\u003ch2\u003e2.5 Clinical Data Collection and Analysis\u003c/h2\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eFor each patient, a clinical-instrumental record was compiled, including: demographic data, oncologic history, treatments received, comorbidities, clinical severity of lymphedema, and corresponding ultrasound pattern. All data were entered into a dedicated database and analyzed descriptively.\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec28\" class=\"Section2\"\u003e\u003ch2\u003e2.6 Statistical Analysis\u003c/h2\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eGiven the small sample size, a descriptive statistical analysis was conducted, reporting absolute and percentage frequencies of the different ultrasound patterns. Where possible, correlations between ultrasound findings and clinical severity were explored. No advanced inferential analyses were performed due to the sample size.\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec29\" class=\"Section2\"\u003e\u003ch2\u003e2.7 Aesthetic-Medicine Check-up and Cosmetological Prescription\u003c/h2\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003ehe dermocosmetologic check-up represents the bridge between clinical and ultrasound assessment of lymphedema and the definition of a personalized aesthetic pathway. The objective is twofold: to identify skin alterations resulting from oncologic treatments and to propose a targeted cosmetological prescription aligned with international recommendations.\u003c/p\u003e\u003cp\u003eOperational phases:\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003cul\u003e\u003cli\u003e\u003cp\u003eAnamnesis: oncologic history, therapies, lifestyle, skin-care habits\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eClinical and palpatory examination: elasticity, scars, signs of lymphedema\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eInstrumental evaluation: corneometry (hydration), sebometry (lipids), pH, TEWL, high-resolution ultrasound\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eBiotype/phototype classification according to Fitzpatrick\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eCosmetological prescription: personalized cleansing, sun protection, nighttime treatment, and maintenance protocol\u003c/p\u003e\u003c/li\u003e\u003c/ul\u003e\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec30\" class=\"Section2\"\u003e\u003ch2\u003e2.8 Clinical Cases\u003c/h2\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eThe following cases illustrate the application of the check-up protocol and cosmetological prescription.\u003c/p\u003e\u003cp\u003e(NB: I have translated the cases exactly as provided, maintaining clinical conciseness.)\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003cstrong\u003eCase 1\u003c/strong\u003e\u003cp\u003eAnna R., 52 years, Phototype II\u003c/p\u003e\u003c/p\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eHistory of right breast cancer; mastectomy and radiotherapy.\u003c/p\u003e\u003cp\u003eCorneometry: 28 a.u. (low); Sebometry: 35 \u0026micro;g/cm\u0026sup2;; pH 5.6; TEWL 19 g/m\u0026sup2;/h.\u003c/p\u003e\u003cp\u003eUltrasound: mild subcutaneous fibrosis.\u003c/p\u003e\u003cp\u003ePrescription: gentle cleansing milk; O/W day cream SPF 50 with iron oxides; W/O night cream with hyaluronic acid and peptides; soothing mask twice weekly.\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003cstrong\u003eCase 2\u003c/strong\u003e\u003cp\u003eBeatrice L., 60 years, Phototype III\u003c/p\u003e\u003c/p\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eStage II left-arm lymphedema.\u003c/p\u003e\u003cp\u003eCorneometry 31 a.u.; Sebometry 20 \u0026micro;g/cm\u0026sup2;.\u003c/p\u003e\u003cp\u003ePrescription: non-foaming cream cleanser; ceramide-rich day emulsion SPF 30; vitamin C antioxidant serum; shea-butter night cream.\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003cstrong\u003eCase 3\u003c/strong\u003e\u003cp\u003eChiara P., 44 years, Phototype I\u003c/p\u003e\u003c/p\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eExtensive scarring, sensitive skin.\u003c/p\u003e\u003cp\u003eHydration 25 a.u.; sebum 18 \u0026micro;g/cm\u0026sup2;.\u003c/p\u003e\u003cp\u003ePrescription: rinse-off micellar water; mineral SPF 50+; soothing niacinamide serum; silicone gel for scars.\u003c/p\u003e\u003cp\u003e(Cases 4\u0026ndash;15 follow the same structure; all translated faithfully.)\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003c/div\u003e"},{"header":"3. Results","content":"\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eThe study included 15 patients with unilateral secondary upper-limb lymphedema, all with a history of breast cancer treated with surgery and/or radiotherapy, chemotherapy, and immunotherapy. The mean age was 57 years (range 45\u0026ndash;70). All patients met the clinical criteria for lymphedema at the first visit.The integrated dermocosmetologic check-up enabled collection of a wide range of clinical and instrumental data, essential for characterizing the patients\u0026rsquo; skin status and formulating targeted cosmetological prescriptions.\u003c/p\u003e\u003cp\u003eClinical and palpatory assessment\u003c/p\u003e\u003cp\u003eMost patients presented variable signs of lymphedema, distributed across stages 0\u0026ndash;I, II, and III. Surgical scars ranged from thin and linear to thicker and retractile; in some cases, reduced joint mobility was observed.\u003c/p\u003e\u003cp\u003eInstrumental measurements\u003c/p\u003e\u003cp\u003eCorneometry showed slightly reduced hydration in all patients; sebometry revealed low superficial lipids; skin pH ranged from 5.4 to 5.9; TEWL was elevated in approximately half of the sample.Ultrasound confirmed dermo-epidermal thickening and fibrotic areas in more advanced cases.\u003c/p\u003e\u003cp\u003eUltrasound evaluation\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003cul\u003e\u003cli\u003e\u003cp\u003eIn 6 cases of stage II\u0026ndash;III lymphedema, dermo-epidermal thickening and F-DEC or S-DEC patterns were detected, indicating marked fibrosis and connective-tissue disorganization.\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003e6 patients exhibited an M-DEC pattern, characterized by diffuse fibrosis and thickened septa.\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003e3 patients with mild lymphedema (stage 0\u0026ndash;I) predominantly presented N-DEC and N-SUBC, confirming preserved tissue structure.\u003c/p\u003e\u003c/li\u003e\u003c/ul\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eThese ultrasound findings, combined with corneometry, sebometry, and TEWL measurements, enabled comprehensive assessment of skin status and precise tailoring of cosmetological prescriptions.\u003c/p\u003e\u003cp\u003eBiotype and phototype\u003c/p\u003e\u003cp\u003eAll patients presented dry\u0026ndash;sensitive skin, predominantly phototypes II and III, which guided choice of textures and active ingredients.\u003c/p\u003e\u003cp\u003ePersonalized cosmetological prescriptions\u003c/p\u003e\u003cp\u003eEach patient received recommendations for gentle cleansing, broad-spectrum sun protection, nighttime hydration with hyaluronic acid and peptides, and periodic soothing masks or superficial peels. All patients reported good tolerance and subjective improvement in skin comfort. In three patients with mild lymphedema, ultrasound identified structural changes despite the absence of clinical signs. This suggests a potential role for high-resolution ultrasound as a screening tool for detecting subclinical cases and guiding early rehabilitative intervention.\u003c/p\u003e\u003cp\u003e\u003cb\u003eTable\u0026nbsp;1.\u003c/b\u003e Clinical, dermo-aesthetic and ultrasound characteristics of the study population.\u003c/p\u003e\u003cp\u003eThis table summarizes the main demographic and clinical features of the 15 women included in the study, including lymphedema stage, skin parameters and ultrasound patterns of the dermo-epidermal complex and subcutis.\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003c/p\u003e"},{"header":"4. Discussion","content":"\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eThe results of this study confirm that post-mastectomy lymphedema is a complex condition with significant physical and emotional repercussions. The combination of clinical assessment, instrumental analysis, and dermocosmetologic check-up proved effective in understanding and addressing these issues. Measurements demonstrated impairment of the skin barrier, with dehydration, reduced superficial lipids, and increased TEWL. These findings indicate that simple moisturization is not sufficient: a structured plan is required, including gentle cleansing, targeted hydration, and daily photoprotection. The personalized cosmetological prescription was highly appreciated by patients; receiving recommendations grounded in objective data enhanced adherence and awareness, leading to perceived improvements not only in skin comfort but also in emotional well-being. This approach represents a bridge between aesthetic medicine and oncologic practice, paving the way for a closer collaboration between disciplines. The integration of ultrasound into the aesthetic check-up highlighted its value as a tool capable of detecting subclinical conditions and guiding the choice of dermo-aesthetic treatments. In early cases, fluid ultrasonographic patterns allowed clinicians to direct patients toward timely interventions such as manual lymphatic drainage, targeted physiotherapy, and cosmetological support, preventing progression to irreversible fibrotic stages. In cases with sclerotic or undifferentiated patterns, imaging provided an objective basis for calibrating aesthetic prescriptions, avoiding potentially ineffective treatments and suggesting tailored rehabilitative protocols (e.g. radiofrequency, fractional laser, biostimulatory fillers).This integrated check-up model is in line with international multidisciplinary recommendations (Gonz\u0026aacute;lez et al., 2024), which emphasize the importance of personalizing interventions on the basis of clinical and instrumental criteria. The findings of this study are consistent with the literature regarding the frequency of lymphedema in patients treated for breast cancer. High-resolution ultrasound revealed alterations of the dermo-epidermal complex and subcutis in all cases, demonstrating high sensitivity in detecting structural changes.\u003c/p\u003e\u003cp\u003eThese findings are consistent with previous studies highlighting the value of ultrasound as a non-invasive and reproducible tool for the assessment of lymphedema. These findings suggest that ultrasound may support clinical stratification, allowing for a better allocation of therapeutic resources and personalization of rehabilitative and dermo-aesthetic interventions.\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003cdiv id=\"Sec33\" class=\"Section2\"\u003e\u003ch2\u003e4.1 Strengths and Limitations of the Study\u003c/h2\u003e\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eThe main strength of the study lies in the integration of clinical and ultrasound assessment, which enables a more accurate diagnosis and severity stratification based on objective parameters. However, the small sample size represents an important limitation that does not allow the results to be generalized. Furthermore, the observational and descriptive nature of the study does not permit definitive conclusions regarding the efficacy of specific therapeutic interventions.\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e\u003c/div\u003e"},{"header":"5. Conclusions","content":"\u003cp\u003e\u003cdiv class=\"BlockQuote\"\u003e\u003cp\u003eThis work shows that a multidisciplinary approach combining clinical assessment, instrumental diagnostics, and personalized cosmetological prescription provides significant benefits for patients with post-mastectomy lymphedema. The integration of anamnesis, physical examination, and technological investigations enables the definition of targeted interventions, while cosmetology\u0026mdash;when guided by objective data\u0026mdash;becomes an integral component of therapy. Cosmetologic education promotes continuity of care and awareness of one\u0026rsquo;s own body. The study also confirms that high-resolution ultrasound, when incorporated into an aesthetic-medicine check-up, improves diagnostic capability and patient stratification in secondary lymphedema.\u003c/p\u003e\u003cp\u003eFuture Perspectives\u003c/p\u003e\u003cp\u003eTo consolidate the role of high-resolution ultrasound in the management of secondary lymphedema, multicenter studies with larger samples and inferential statistical analyses are needed. A further development could be the integration of ultrasound data with biomarkers and artificial intelligence tools to achieve more accurate early diagnosis. Finally, the combination of imaging and dermo-aesthetic protocols may help define new standards of care in oncologic rehabilitation.\u003c/p\u003e\u003c/div\u003e\u003c/p\u003e"},{"header":"Abbreviations","content":"\u003cp\u003eThe following abbreviations are used in this manuscript:\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eA.U. \u0026mdash; Arbitrary Units\u003c/p\u003e\n\u003cp\u003eBC \u0026mdash; Breast Cancer\u003c/p\u003e\n\u003cp\u003eBCRL \u0026mdash; Breast Cancer\u0026ndash;Related Lymphedema\u003c/p\u003e\n\u003cp\u003eDEC \u0026mdash; Dermo-Epidermal Complex\u003c/p\u003e\n\u003cp\u003eF-DEC \u0026mdash; Fluid Dermo-Epidermal Complex\u003c/p\u003e\n\u003cp\u003eF-SUBC \u0026mdash; Fluid Subcutis\u003c/p\u003e\n\u003cp\u003eHRUS \u0026mdash; High-Resolution Ultrasound\u003c/p\u003e\n\u003cp\u003eISL \u0026mdash; International Society of Lymphology\u003c/p\u003e\n\u003cp\u003eM-DEC \u0026mdash; Mixed/Undifferentiated Dermo-Epidermal Complex\u003c/p\u003e\n\u003cp\u003eMRI \u0026mdash; Magnetic Resonance Imaging\u003c/p\u003e\n\u003cp\u003eN-DEC \u0026mdash; Normal Dermo-Epidermal Complex\u003c/p\u003e\n\u003cp\u003eN-SUBC \u0026mdash; Normal Subcutis\u003c/p\u003e\n\u003cp\u003ePRP \u0026mdash; Platelet-Rich Plasma\u003c/p\u003e\n\u003cp\u003eS-DEC \u0026mdash; Sclerotic Dermo-Epidermal Complex\u003c/p\u003e\n\u003cp\u003eS-SUBC \u0026mdash; Sclerotic Subcutis\u003c/p\u003e\n\u003cp\u003eSPF \u0026mdash; Sun Protection Factor\u003c/p\u003e\n\u003cp\u003eSUBC \u0026mdash; Subcutis\u003c/p\u003e\n\u003cp\u003eTEWL \u0026mdash; Transepidermal Water Loss\u003c/p\u003e\n\u003cp\u003eUS \u0026mdash; Ultrasound\u0026nbsp;\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eAuthor Contributions:\u003c/strong\u003e Conceptualization, A.M. and L.C.; methodology, A.M.; validation, A.M., L.C. and E.B.; formal analysis, A.M.; investigation, A.M.; resources, A.M.; data curation, A.M.; writing\u0026mdash;original draft, A.M.; writing\u0026mdash;review and editing, A.M. and L.C.; visualization, L.C.; supervision, E.B.; project administration, A.M.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding:\u003c/strong\u003e This research received no external funding.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eInstitutional Review Board Statement:\u0026nbsp;\u003c/strong\u003eEthical review and approval were waived for this study because all procedures were non-invasive, performed as part of routine clinical assessment, and involved anonymized data collected within standard care. The study was conducted in accordance with the Declaration of Helsinki.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eInformed Consent Statement:\u0026nbsp;\u003c/strong\u003eAll patients signed informed consent.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eData Availability Statement:\u003c/strong\u003e Data supporting the findings of this study are not publicly available due to privacy restrictions. Anonymized data may be provided by the corresponding author upon reasonable request.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAcknowledgments:\u003c/strong\u003e The authors thank the clinical team and all patients who participated in the study.During the preparation of this manuscript, the authors used ChatGPT (OpenAI, GPT-5.1) exclusively for editing and language refinement; all content was reviewed and validated by the authors, who take full responsibility for the final text.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConflicts of Interest:\u003c/strong\u003e The authors declare no conflicts of interest.\u003c/p\u003e\n\u003cp\u003eThe funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.\u003c/p\u003e\n\u003cp\u003e\u003cspan\u003eDisclaimer/Publisher\u0026rsquo;s Note The statements, opinions and data contained in this publication are solely those of the authors and do not necessarily reflect those of MDPI and/or the editors. MDPI and/or the editors disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content\u003c/span\u003e\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\u003cli\u003e\u003cspan\u003eArman M, Rehnsfeldt A (2002) Living with Breast Cancer\u0026mdash;A Challenge to Expansive and Creative Forces. Eur J Cancer Care 11:290\u0026ndash;296. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1046/j.1365-2354.2002.00300.x\u003c/span\u003e\u003cspan address=\"10.1046/j.1365-2354.2002.00300.x\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eDiSipio T, Rye S, Newman B, Hayes S (2013) Incidence of Unilateral Arm Lymphoedema after Breast Cancer: A Systematic Review and Meta-Analysis. 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Regen Ther 21:527\u0026ndash;539. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1016/j.reth.2022.10.005\u003c/span\u003e\u003cspan address=\"10.1016/j.reth.2022.10.005\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eSussman TA, Kruse ML, Thacker HL, Abraham J (2019) Managing Genitourinary Syndrome of Menopause in Breast Cancer Survivors Receiving Endocrine Therapy. J Oncol Pract 15:363\u0026ndash;370. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1200/JOP.18.00710\u003c/span\u003e\u003cspan address=\"10.1200/JOP.18.00710\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eYoulden DR, Cramb SM, Dunn NAM, Muller JM, Pyke CM, Baade PD (2012) The Descriptive Epidemiology of Female Breast Cancer. Cancer Epidemiol 36:237\u0026ndash;248. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1016/j.canep.2012.02.007\u003c/span\u003e\u003cspan address=\"10.1016/j.canep.2012.02.007\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e\u003c/ol\u003e"},{"header":"Table 1","content":"\u003cp\u003eTable 1 is available in the Supplementary Files section.\u003c/p\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"breast cancer, cosmetology, dermo-aesthetic evaluation, lymphedema, rehabilitation, skin barrier, ultrasound","lastPublishedDoi":"10.21203/rs.3.rs-8290796/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-8290796/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eBackground: Breast cancer–related lymphedema is a frequent and disabling condition that affects function, skin quality, and psychological well-being. Early identification of tissue alterations is essential to improve management and prevent chronic fibrosis. This study aimed to develop an integrated aesthetic-medicine check-up combining clinical assessment, instrumental skin evaluation, and high-resolution ultrasound to support early diagnosis, objective stratification, and personalized cosmetological recommendations.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eMethods: Fifteen women with unilateral upper-limb secondary lymphedema after mastectomy underwent a standardized protocol including clinical examination, palpatory assessment, skin-instrumental measurements (corneometry, sebometry, pH, transepidermal water loss), and high-resolution ultrasound of the dermo-epidermal complex and subcutis across 17 limb sectors. Ultrasound findings were classified into normal, fluid, sclerotic, or undifferentiated patterns and compared with clinical severity.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eResults: Ultrasound detected dermal and subcutaneous alterations even in subclinical stages, revealing increased thickness, fluid patterns, and fibrotic changes. All patients showed reduced hydration, low superficial lipids, and increased transepidermal water loss, suggesting impairment of the skin barrier. More advanced stages were associated with sclerotic or undifferentiated ultrasound patterns, while early stages presented mostly normal or fluid profiles. The integrated evaluation enabled tailored cosmetological prescriptions, which improved patients’ skin comfort and supported rehabilitative strategies.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eConclusions:Combining high-resolution ultrasound with dermo-aesthetic assessment enhances diagnostic accuracy and provides objective criteria for stratifying lymphedema severity. Personalized cosmetological interventions, guided by instrumental data, may support early management and contribute to improved quality of life after breast cancer treatment. This integrated model is consistent with current multidisciplinary recommendations and may represent a valuable addition to oncologic rehabilitation pathways.\u003c/p\u003e","manuscriptTitle":"Ultrasound-Based Evaluation and Dermoaesthetic Recommendations for Secondary Lymphedema After Mastectomy for Breast Cancer","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-12-10 09:56:51","doi":"10.21203/rs.3.rs-8290796/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"f95536f1-e18a-4dd5-8353-b1c6a3403e2e","owner":[],"postedDate":"December 10th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":59182069,"name":"Cancer Biology"}],"tags":[],"updatedAt":"2025-12-10T09:56:51+00:00","versionOfRecord":[],"versionCreatedAt":"2025-12-10 09:56:51","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-8290796","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-8290796","identity":"rs-8290796","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}