Study on Induction of Apoptosis by Fugongning in Endometriotic Cells

Objective:To explore the mechanism of chinese drugs Fugongning on cures of Endometriosis at the molecular level, and to investigate whether apoptosis is induced in endometriosis by the medicine. Methods: After making EMT animal model the rats were treated with Fugongning. Danazol and both medicines. Then apoptotic cells were detected by flow cytometry (FCM).Result:1. On gross examination the size of transplant in treated groups was significantly decresed as compared with that of control ( P 0 05). The growth of Endometriotic cells were inhibited. 2.The apoptosis index in Fugongning, Danazol and the both medicine group was significantly higher than that of control ( P 0 )1).3.After treatment with Fugongning, Danazol and both medicines, there were apoptotic peaks in cell cycle analysis on FCM. The cells in S phase were obvionsly increased ( P 0 01),which indicated that cell cycles were hindered in S phase. 4. The serum E 2 level in Fugongning, Danazol and the both medicine group was significantly lower that that of control ( P 0 05). Conclusion: This study indicates that Fugongning can induce apoptosis of Endometriotic cells. The possible mechanism is from decreasing the serum E 2 level to suppress bc1 2 expression in endometriotic cells.