Mechanisms of Atractylodes macrocephala Koidz. on therapy to diarrheal disease: A systematical review and meta-analysis of preclinical evidence

Mechanisms of Atractylodes macrocephala Koidz. on therapy to diarrheal disease: A systematical review and meta-analysis of preclinical evidence | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Mechanisms of Atractylodes macrocephala Koidz. on therapy to diarrheal disease: A systematical review and meta-analysis of preclinical evidence Pancen Ran, Fengxian Jiang, Jingjiang Lai, Yang Shu, Jing Zhao, and 5 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3847612/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background Atractylodes macrocephala Koidz. (AMK) is a traditional Chinese medicine used for the treatment of various diseases. Atractylenolide and polysaccharide from Atractylodes macrocephala Koidz. (PAMK) are the representative bioactive components in AMK. There have been many in vivo studies on AMK for the treatment of diarrhea, the most common gastrointestinal disorder worldwide and the high mortality rate. The aim of this study is to assess the effectiveness of AMK in treating diarrhea using preclinical evidence and meta-analysis. Additionally, the primary mechanisms by which AMK acts against diarrhea are summarized. Methods We systematically searched PubMed, WOS, Embase, Cochrane Library, WanFang, CNKI, Cqvip and CBM databases and other databases from January 1, 2000, to March 10, 2023, to evaluate the animal experiments of AMK for the treatment of diarrhea. Finally, 23 animal experiments were included. Thirteen outcome measures, including overall condition(body weight, diarrhea score, disease activity index), immune-related indicators (colon length, spleen index, histopathological score, SIgA), pro-inflammatory factors(IL-1β, IL-6, IL-17,TNF-α), anti-inflammatory factors(IL-10) and MPO were obtained by using Review Manager 5.4 software for meta-analysis. Results Except for the indicator of spleen index, which was not significant difference, it was significantly decreased in the indicator of diarrhea score, disease activity index, histopathological score and significantly elevated in the indicator of body weight, colon length. It showed that AMK could improve immune function in diarrhea. Meanwhile AMK significantly down-regulated pro-inflammatory factors, IL-1β, IL-6, IL-17, TNF-α and MPO, up-regulated anti-inflammatory factors IL-10, indicating that AMK may have anti-inflammation and anti-oxidation effects. Conclusion AMK exhibits protective effects against diarrhea through immunoregulation, anti-inflammation, anti-oxidation, and regulation of intestinal flora. These findings provide preclinical evidence supporting the application of AMK in diarrhea treatment. Atractylodes macrocephala Koidz. Diarrhea Mechanisms Systematic review Meta-analysis Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3847612","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":266406426,"identity":"d4a4371f-1c08-47ce-a993-5edc9aa02ce7","order_by":0,"name":"Pancen Ran","email":"","orcid":"","institution":"Shandong Provincial Hospital Affiliated to Shandong First Medical University","correspondingAuthor":false,"prefix":"","firstName":"Pancen","middleName":"","lastName":"Ran","suffix":""},{"id":266406427,"identity":"10762e90-55f6-4fcb-b46c-ec4d79e896a1","order_by":1,"name":"Fengxian Jiang","email":"","orcid":"","institution":"Shandong Provincial 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therapy to diarrheal disease: A systematical review and meta-analysis of preclinical evidence","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Atractylodes macrocephala Koidz., Diarrhea, Mechanisms, Systematic review, Meta-analysis","lastPublishedDoi":"10.21203/rs.3.rs-3847612/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-3847612/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eBackground\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cem\u003eAtractylodes macrocephala\u003c/em\u003e Koidz. (AMK) is a traditional Chinese medicine used for the treatment of various diseases. Atractylenolide and polysaccharide from \u003cem\u003eAtractylodes macrocephala\u003c/em\u003e Koidz. (PAMK) are the representative bioactive components in AMK. There have been many in vivo studies on AMK for the treatment of diarrhea, the most common gastrointestinal disorder worldwide and the high mortality rate. The aim of this study is to assess the effectiveness of AMK in treating diarrhea using preclinical evidence and meta-analysis. Additionally, the primary mechanisms by which AMK acts against diarrhea are summarized.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethods\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eWe systematically searched PubMed, WOS, Embase, Cochrane Library, WanFang, CNKI, Cqvip and CBM databases and other databases from January 1, 2000, to March 10, 2023, to evaluate the animal experiments of AMK for the treatment of diarrhea. Finally, 23 animal experiments were included. Thirteen outcome measures, including overall condition(body weight, diarrhea score, disease activity index), immune-related indicators (colon length, spleen index, histopathological score, SIgA), pro-inflammatory factors(IL-1β, IL-6, IL-17,TNF-α), anti-inflammatory factors(IL-10) and MPO were obtained by using Review Manager 5.4 software for meta-analysis.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eExcept for the indicator of spleen index, which was not significant difference, it was significantly decreased in the indicator of diarrhea score, disease activity index, histopathological score and significantly elevated in the indicator of body weight, colon length. It showed that AMK could improve immune function in diarrhea. Meanwhile AMK significantly down-regulated pro-inflammatory factors, IL-1β, IL-6, IL-17, TNF-α and MPO, up-regulated anti-inflammatory factors IL-10, indicating that AMK may have anti-inflammation and anti-oxidation effects.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConclusion\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAMK exhibits protective effects against diarrhea through immunoregulation, anti-inflammation, anti-oxidation, and regulation of intestinal flora. These findings provide preclinical evidence supporting the application of AMK in diarrhea treatment.\u003c/p\u003e","manuscriptTitle":"Mechanisms of Atractylodes macrocephala Koidz. on therapy to diarrheal disease: A systematical review and meta-analysis of preclinical evidence","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-01-12 10:59:52","doi":"10.21203/rs.3.rs-3847612/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"f1fd5c10-ce95-418b-bda7-5e62b3a94c37","owner":[],"postedDate":"January 12th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2024-01-31T09:01:52+00:00","versionOfRecord":[],"versionCreatedAt":"2024-01-12 10:59:52","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-3847612","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-3847612","identity":"rs-3847612","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}