The Effect of post Chlamydia Trachomatis Infection treatment on Reactive Oxygen Species and Sperm Parameters of Infertile Men

ABSTRACT Background Chlamydia trachomatis (CT) infection is often mentioned as a silent disease. Reactive oxygen species (ROS) can also cause Sperm apoptosis and have negative impact on Sperm parameters. The objectives of this study were to elucidate the association between Sperm parameters and ROS caused by CT infections resulting in male Infertility as well as evaluating the role of antibiotic therapy. Materials and methods A total of 848 infertile males having normal and abnormal Sperm parameters were included. After Semen sampling, the CT IgA were measured by Elisa and confirmed by Nested PCR. ROS was determined by Chemiluminescence. After treatment under the direct supervision of the private urologists. Then, the second Semen samples were taken and subjected to tests on Sperm parameters and ROS levels as assessed again. Results The levels of ROS and morphology were improved following the treatments (P<0.05). Antibiotic therapy due to CT infection, could reduce ROS, improve normal morphology and recover some of Semen parameters. Conclusions Our findings indicate that CT infection and Sperm parameters were associated with the rate of ROS in infertile men. However, after treatment, ROS value dropped allowing the recovery of certain Sperm parameters. Antibiotic therapy can improve some Semen quality parameters and treat the male Infertility. Reza Azmakan, [email protected] , Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran, https://orcid.org/0000-0001-6718-3348 .