Gamma Radiation Assisted Green Synthesis of Silver Nanoparticles-Loaded (Polyacrylamide/Chitosan) Hydrogel Nanocomposites and their Using as Antifungal against Candida albicans and Anti-Cancer Modulator

Gamma Radiation Assisted Green Synthesis of Silver Nanoparticles-Loaded (Polyacrylamide/Chitosan) Hydrogel Nanocomposites and their Using as Antifungal against Candida albicans and Anti-Cancer Modulator | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Gamma Radiation Assisted Green Synthesis of Silver Nanoparticles-Loaded (Polyacrylamide/Chitosan) Hydrogel Nanocomposites and their Using as Antifungal against Candida albicans and Anti-Cancer Modulator Salwa A. Khalil, Ahmed Awadallah-F, Mervat R. Khaffaga, Rasha Mohammad Fathy, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4686117/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 28 Oct, 2024 Read the published version in Scientific Reports → Version 1 posted 11 You are reading this latest preprint version Abstract Silver nanoparticles-loaded hydrogel nanocomposites are employed for medicinal and pharmaceutical applications.Hydrogel nanocomposites were synthesized from acrylamide (Am), chitosan (CS) and AgNO 3 using gamma irradiation. Different parameters were exploited in synthesis of silver nanoparticles-laoded hydrogel nanocomposites of (PAm/CS)-AgNPs such as effect of irradiation dose and effect of CS concentration. Diverse techniques were used to characterize hydrogel nanocomposites; Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), X-ray diffraction (XRD), Transmission Electron Microscopy (TEM), Energy dispersive X-ray (EDX) and scanning electron microscopy SEM. Results confirmed the formation of silver nanoparticles-loaded hydrogel nanocomposites of (PAm/CS)-AgNPs. Antifungal activity of (PAm/CS)-AgNPs hydrogel nanocomposites on viability of C. albicans was assessed. Results showed the efficient microbial inhibition activity of treatment against C. albicans comparable to control. Also, (PAm/CS)-AgNPs hydrogel nanocomposite against cervical cancer HeLa cell line was studied. Cytotoxicity of (PAm/CS)-AgNPs hydrogel nanocomposites on prior cancer cell line led to the prohibition of the cell growth evalauted by the MTT test. The HeLa cancer cell is traeted by (PAm/CS)-AgNPs for 48h showed a possible apoptotic activity by noticeable up-regulation of p53 gene expression. In addition, anticancer activity was examined by down-regulation of platelet-based growth paramater receptor beta (PDGFR-β), Bcl2, Cathepsine, and MMP-2 gene expression. Physical sciences/Materials science Physical sciences/Materials science/Structural materials Physical sciences/Materials science/Structural materials/Composites Hydrogel Nanocomposite Gamma irradiation Antimicrobial Anti-cancer Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 28 Oct, 2024 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 29 Jul, 2024 Reviews received at journal 23 Jul, 2024 Reviews received at journal 12 Jul, 2024 Reviewers agreed at journal 12 Jul, 2024 Reviewers agreed at journal 12 Jul, 2024 Reviewers agreed at journal 12 Jul, 2024 Reviewers invited by journal 12 Jul, 2024 Editor assigned by journal 12 Jul, 2024 Editor invited by journal 11 Jul, 2024 Submission checks completed at journal 08 Jul, 2024 First submitted to journal 04 Jul, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4686117","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":332949265,"identity":"7b9606d0-932d-4869-afac-1e0d966b1232","order_by":0,"name":"Salwa A. 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Different parameters were exploited in synthesis of silver nanoparticles-laoded hydrogel nanocomposites of (PAm/CS)-AgNPs such as effect of irradiation dose and effect of CS concentration. Diverse techniques were used to characterize hydrogel nanocomposites; Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), X-ray diffraction (XRD), Transmission Electron Microscopy (TEM), Energy dispersive X-ray (EDX) and scanning electron microscopy SEM. Results confirmed the formation of silver nanoparticles-loaded hydrogel nanocomposites of (PAm/CS)-AgNPs. Antifungal activity of (PAm/CS)-AgNPs hydrogel nanocomposites on viability of \u003cem\u003eC. albicans\u003c/em\u003e was assessed. Results showed the efficient microbial inhibition activity of treatment against \u003cem\u003eC. albicans\u003c/em\u003e comparable to control. Also, (PAm/CS)-AgNPs hydrogel nanocomposite against cervical cancer HeLa cell line was studied. 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