Light-dependent changes in the higher-order DNA structure of the cyanobacterium Synechocystis sp. PCC6803

Abstract Chromosome spatial organization plays critical roles in transcriptional regulation and DNA protection. In cyanobacteria—photosynthetic bacteria that experience dramatic fluctuations in light intensity—chromosome reorganization could facilitate rapid transcriptional reprogramming and protect DNA from photodamage. However, chromosome organization in these polyploid organisms has remained technically challenging to observe, leaving light-dependent responses unexplored. Here, we show that higher-order chromosome organization in Synechocystis sp. PCC 6803 is associated with light intensity, revealing a previously unrecognized light-dependent adaptation in cyanobacteria. We established fluorescence in situ hybridization (FISH) methods for this model cyanobacterium carrying multi-copy genomes, together with a computational pipeline to assign paired FISH signals to individual genome copies. The slope relating genomic and spatial distance was steeper under standard conditions (β = 0.972 nm/kbp, R² = 0.12) than under high-light conditions (β = 0.450 nm/kbp, R² = 0.02), indicating that local chromosome organization is substantially disrupted by elevated light intensity. The spatial distribution of the multiple genome copies also differed between conditions, independently supporting condition-dependent chromosome reorganization. Hi-C analysis corroborated these findings, revealing reduced chromosomal interactions within the 10–100 kbp range under high-light conditions. Together, these results demonstrate that light intensity is a previously unrecognized determinant of higher-order chromosome organization in a photosynthetic bacterium. Competing Interest Statement The authors have declared no competing interest. Footnotes Abstract, introduction, and discussion have been revised.