Investigating Mycoplasma wenyonii and Candidatus Mycoplasma haematobovis coinfection patterns and risk factors in cattle from southwestern France reveals strain-specific traits

Investigating Mycoplasma wenyonii and Candidatus Mycoplasma haematobovis coinfection patterns and risk factors in cattle from southwestern France reveals strain-specific traits | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Investigating Mycoplasma wenyonii and Candidatus Mycoplasma haematobovis coinfection patterns and risk factors in cattle from southwestern France reveals strain-specific traits Chloé Saada, Renaud Maillard, Claire Pelletier, Marie-Claude Hygonenq, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9096969/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Hemotropic mycoplasmas (hemoplasmas) infecting cattle were first detected in France in 2019 during a clinical outbreak, but their prevalence both at the species and strain levels remains unknown. This study therefore aimed to determine the prevalence of hemoplasmas and their coinfections, and to identify associated risk factors using a fixed-effect logistic regression model. Detection was performed using 16S rRNA qPCR, as well as three strain-specific rnpB qPCR assays targeting Candidatus Mycoplasma haematobovis (CMh, formerly Ca . M. haemobos), Mycoplasma wenyonii strain Mexico (Mex), and Mycoplasma wenyonii strain Massachusetts (Mass). A total of 1011 cattle were sampled, of which 88.7% (897/1011) tested positive by 16S rRNA qPCR. Prevalence rates for CMh, Mex, and Mass were 67.7% (684/1011), 65.9% (666/1011), and 19.8% (200/1011), respectively. Concordance between 16S rRNA qPCR and strain-specific rnpB qPCR was 92% (931/1011). Coinfections were detected in 67.8% (565/833) of infected animals, with the CMh + Mex combination being the most frequent. The Mass strain was predominantly found in triple infections. Risk factors for infection included coinfections, age (except for Mass), and climatic conditions for CMh and Mass. Further studies are needed to elucidate transmission routes and pathogenicity mechanisms of hemoplasmas. Mycoplasma wenyonii Candidatus Mycoplasma haematobovis haemoplasma coinfections cattle France Full Text Additional Declarations No competing interests reported. Supplementary Files Supplementarydata.docx Hemoplasmadatabase.xlsx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-9096969","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":606646738,"identity":"172e88e3-cd8a-4b07-9a54-63999c7983ee","order_by":0,"name":"Chloé Saada","email":"","orcid":"","institution":"Univ Toulouse, ENVT, INRAE, IHAP","correspondingAuthor":false,"prefix":"","firstName":"Chloé","middleName":"","lastName":"Saada","suffix":""},{"id":606646739,"identity":"cfb600e3-3222-4379-9b85-34ff6d76975d","order_by":1,"name":"Renaud Maillard","email":"","orcid":"","institution":"Univ Toulouse, ENVT, INRAE, 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