Relationship between blood Lead status and anemia in Ugandan children with malaria infection

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Vuzi, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-52787/v3 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 14 Nov, 2020 Read the published version in BMC Pediatrics → Version 3 posted 2 You are reading this latest preprint version Show more versions Abstract Background In Uganda, childhood anemia remains a health challenge and is associated with malaria infection as well as iron deficiency. Iron deficiency is intertwined with nutritional status, age and other comorbidities including helminths and Lead toxicity. Environmental Lead levels accounts for one’s blood Lead (BL) levels. Blood Lead competitively blocks iron absorption, inhibits hemoglobin (Hb) biosynthesis and elevates free erythrocyte protoporphyrin (FEP) levels. Lead toxicity’s contribution towards anemia pathogenesis, especially during malaria infection has not been studied. Concomitant exposure to both malaria infection and Lead pollution, exacerbates the anemia status. This study therefore aimed at expounding the anemia status of these Ugandan children aged under 5years who are exposed to both malaria infection and environmental Lead pollution. Methods Briefly, venous blood samples from 198 children were microscopically assayed for malaria parasite density (PD), and hemoglobin (Hb) concentrations using the cyanmethemoglobin method, while BL and FEP levels were determined by the standard atomic absorption spectrophotometric and fluorometric methods respectively. Results One hundred and fifty-one (76.3%) of the children analyzed had moderate anemia (Hb 5 g/dL) with Means of BLL=8.6 µg/dL, Hb =7.5 g/dL, FEP/Hb =8.3 µg/g and PD =3.21×103 parasites / µL, while eight (4%) were severely anemic (<5 g/dL). Regression analysis and statistical correlation between PD and Hb (r = -0.231, R 2 = 0.15 P-value < 0.001) was negative and weak as compared to that between FEP/Hb and Hb (r = -0.6, R 2 =0.572 P-value=0.001). Conclusion Based on the study’s findings, we conclude that BL significantly contributes to the pathogenesis of anemia and therefore its co-existence with malaria infection in the host exacerbates the anemia status. Trial registration Not applicable Pediatrics Blood Lead Malaria parasites Anemia Iron deficiency Free erythrocyte protoporphyrins Figures Figure 1 Figure 2 Figure 3 Background Like many malaria holoendemic developing countries, Uganda is faced with health-threatening diseases including anemia. Majority (60%) of the Uganda’s urban poor live in social disadvantaged slums like Katanga, Kampala [1, 2]. In such areas people live next to industries, workshops, motor garages, metal crafts yards, battery recycling plants, mosquito breeding grounds, and landfills predisposing them to frequent malaria infections as well as Lead intoxication. Several studies report elevated environmental Lead levels in water sources, soils, foodstuffs and air around Kampala city [3]. Malaria and Lead pollution geographically overlap, with water acting as sinks to Lead contaminated runoffs as well as bleeding ground for mosquitos. According to a study by [4], malaria infection accounts for up to 40% of all hospital outpatient visits, 25% of all hospital admissions, and 14% of all hospital deaths [4, 5] despite government efforts to curb its transmission [6]. Already fifty-three percent of children under the age of 5 years in Katanga area are reported to be anemic [6-9] this being attributed to high malaria prevalence. Although, malaria infections remain the key cause of anemia in Uganda, other neglected yet significant confounders contributing to its pathogenesis include Lead poisoning, nutritional status, and helminths among others [6-9]. Lead exposure accounts for an individual’s blood Lead level (BLL) and is more evident in developing fetus where it directly affects the hematopoietic system. Ninety-nine percent of all the BL sink in the erythrocytes and 80% of it bind the δ-aminolevulinic acid dehydratase (δ-ALAD) enzyme. This enzyme is believed to catalyze the formation of porphobilinogen from δ-aminolevulinic acid (ALA) [10,]. During Lead intoxication, BL inhibits activity(s) of ALAD and limit the transfer of iron from endosomes to the cytoplasm resulting into fragile cell membrane which in turn shorten the lifespan of the circulating erythrocytes [11-13]. Blood Lead further triggers a reduction in red blood cells (RBC) production by specifically inhibiting Ferrochelatase a mitochondrial enzyme that catalyzes the insertion of iron into protoporphyrin during heme formation. Aminolevulinic acid synthetase (ALAS) a mitochondrial enzyme that catalyzes the formation of aminolevulinic acid (ALA) from succinyl CoA and glycine is also affected by BL [13-16]. In a normal heme synthesis system, the rate of iron formation and utilization is well balanced, however, this equilibrium is disturbed with insufficiency of iron. High malaria burden, overproduction of protoporphyrin, iron deficiency, inhibited ALAD and impaired ferro chelatase activity accounts for anemia pathogenesis. During malaria infection, intrinsic and extrinsic challenges are elevated and they induce iron deficiency anemia whose persistence elevates the rate at which Lead is absorbed [17-19]. In addition to other symptoms, malaria infection becomes fatal with severe anemia. Coupled with severe anemia, malaria is a result of massive erythrocyte s lysis because of raising parasite density which in turn causes rupture of parasitized and non-parasitized red cells. Its persistence activates the splenic and other macrophages activities for phagocytosis [20] resulting into severe anemia. Reduced cellular iron concentrations on the other hand enhances Lead’s effects on major synthesis reactions. For example, ferrochelatase enzyme which is specifically sensitive to low iron levels is affected by lead toxicity (21). Also zinc instead of iron is incorporated into protoporphyrin (PPN) resulting in elevated levels of zinc protoporphyrin (ZPP) [22]. Zinc protoporphyrin or FEP concentrations are key biomarkers of heme synthesis status, and therefore, their elevation in concentration is associated with iron deficiency due to heme synthesis disorders [23, 24]. Since red cells are at the centre of anemia pathogenesis, as well as home to both the malaria parasites and BL, their co-existence propagates the host cell’s survival challenges. The severity of anemia is dependent on the host's age and nutrition status [25, 26]. In addition, the combined aftermath of malaria parasites and BL among individuals living in malaria-endemic regions heighten the progression to severe anemia. This study therefore aimed at expounding the anemia status of these Ugandan children who are exposed to both malaria infection and environmental lead pollution. In this study, hemoglobin (Hb) concentration was used as a measure of one’s anemia status, and FEP/Hb ratio for heme synthesis status. Methods This was a cross-sectional study on children aged 6 -60months living in Katanga area- Kampala city. The children were first screened for Plasmodium parasite infection before recruitment using rapid diagnostic kits. Children with malaria negative test, HIV positive test, blood transfusion history and signs of malnutrition were excluded from the study. Five (5) mls of venous blood from 198 malaria positive children were collected into EDTA by Qualified Nurses and Technicians. The samples were then transported on ice to Makerere University, Biochemistry Department L a boratory, stratified according to malaria parasite density and kept at 4 0 C awaiting various analysis and determinations. Determination of Parasite density by thick smear method Thick smears were prepared as described by [27], air dried, stained with 10% Leishman without fixing and examined under a CX 21 Olympus microscope. Five hundred leukocytes plus the number of malaria parasites seen in the same field were recorded. The number of malaria parasites per microliter (parasite/µL) of blood was expressed as the reciprocal of the mean counts in the three slides divided by the leukocyte counts, multiplied by a factor of 8000 i.e. Determination of blood Lead levels by atomic absorption spectrometry Blood Lead levels were determined following a method described by [28] using an atomic absorption spectrophotometer (Agilent 2000 series) equipped with a graphite tube atomizer and deuterium background correction facility. A hollow-cathode Lead lamp with a working current of 5mA, 283.3 nm spectral line and 0.5 nm bandwidth was used. Aliquots (500 µl) of whole blood melted with 1.2 ml of 0.5% Triton X-100 and 1% (NH 4 ) 2 HPO 4 solution were added to 1.8 ml of deionized water and 1.5 ml of 20% Trichloroacetic acid (TCA) and vortex mixed. The samples were centrifuged for 20 min at 5000 rpm and 10 µl of the supernatant injected onto the graphite tube. The calibration curve was drawn using a standard addition method as described in M-572 of INSPQ’s Toxicology Laboratory method. Fluorospectrophotometric quantification of free erythrocyte protoporphyrins The FEP was measured following a method described by [29] using a fluorospectrophotometer set at 405nm excitation 610nm emissions. The porphyrins were extracted by adding 20 µl aliquots of whole blood to a solution containing 100 µL of 10% ammonium sulfate and 5% celite and vortex mixed for 10sec. 400 µl of 95% ethanol was then added and vortex mixed for more 20 seconds. This was followed by addition of 600 µl of acetone and further vortex mixing for 20 seconds. All the samples were put on an ice bath for 20 min, vortex mixed for 20 sec. and centrifuged at 4°C for 10 min. After which the supernatants were harvested into small borosilicate tubes and aliquots of 300 µl mixed with 300 µl of a solution containing propylene glycol and 1.5 N HCI, (4:1), let to stand for 20 min before reading at 405 nm excitation and 610 emissions. The FEP blood concentration was calculated using the following formulae; Where Fz is the sample fluorescence, Cs concentration of the standard, Fs is the fluorescence of the standard, 2.7 is the final volume of HCl phase, 100 is the conversion factor to 100ml of extract, 1.1 conversion factor for protoporphyrin measured against a coproporphyrin standard and 0.2 (20µl) is the original blood volume measured. The FEP/Hb ratio expressed as microgram Hb per gram Hb was calculated by dividing the FEP/dL RBC by gram Hb/dL. Colorimetric determination of hemoglobin levels by blood cyanmethemoglobin reaction Hemoglobin levels were determined by cyanmethemoglobin reaction method [30]. Aliquots 100µl of samples were made to a total volume of 1000µl with reaction solution containing 200mg of hexacyanoferrate III, 50mg of potassium cyanide, 140mg of potassium hydrogen phosphate and 1ml of Triton X-100 in a liter of distilled water. Then incubated for 15 min at room temperature before reading at 540 nm, with the blank being the reaction reagent. Then 500 µl of standard hemoglobin standard (0.7mg/ml) was diluted with 500 µl of the same reagent, treated as above and readings taken. The Hb concentration in g/dl was calculated using formula; Results Of the 198 children enrolled in the study, 8/198 (4%) were severely anemic with 10g Hb/dL (WHO cut off reference), mean parasite density =1.7×10 3 parasites/ µL, mean BLL < 2µg/dL, Mean FEP/Hb =7.4±2.9 μg /g. The details of the distribution are shown in Table 1 below Table.1 Distribution of parasite density, BLL, FEP/Hb and hemoglobin levels among 198 study participants. No. of samples, N Parasite density/ µL (×103) Mean BLL (µg/dL) Mean FEP/Hb (μg /g) Mean Hb (g/ dL) 16 0 .1–1.1 10.3±1.9 7.9±3 7.0± 3.2 39 1.2–2.2 < 2 0.6 10.4±2.7 42 2.3–3.3 10.3±1.7 2.9±3.2 8.5±3.1 35 3.4–4.4 7.9±2.1 3.9±2.8 9.2±3.0 34 4.5–5.5 7.9±1.8 8.4±3.2 9.3±4.1 23 5.6–6.6 6.4±1.8 4.9±2.7 6.8±3.3 8 6.7–7.7 9.7±5.2 7.4±2.0 4.9±2.9 1 7.8–8.8 5.1 5.5 6.7 N=198 Mean= 1.7×10 3 Mean= 8.6 Mean= 4.4 Mean= 8.7 Hb=Hemoglobin, BLL = Blood Lead levels, FEP=Free erythrocyte protoporphyrin Table 2. Correlation coefficients r and p-values of different interacting variables Discussion Several cellular biomarkers are used to measure the extent and effects of blood Lead (BL) on various biochemical systems. This study explored the levels of Free erythrocyte porphyrins (FEP) as a biomarker of heme biosynthesis disorder among the study participants. The heme biosynthesis process is inactivated by either iron deficiency, failed iron regulatory system or inhibited δ-aminolevulinic acid dehydratase (δ-ALAD) enzyme [31]. Free erythrocyte porphyrin levels of ˃50µg/ dL are important indicator of heme biosynthesis disorder. Common causes of this disorder include iron deficiency due to malnutrition and poor iron absorption, elevated hepcidin levels, and ALAD inhibition by blood Lead [16-18,20, 22, 29]. Findings of this study (Table 1) indicate that 88.2% (n=175) of all study participants were moderately anemic Hb ˃5g/dL < 10g/dL. Of the 175 participants, 111 (56.6%) had normal functioning biosynthesis system (FEP levels ˃50µg/ dL), although with moderate anemia. Therefore, the observed low levels of Hb, cannot be associated with iron deficiency. The candid cofounding factors for the observed low Hb levels therefore are oxidative stress and accumulated erythrocytic pyrimidine nucleotides. Oxidative may be caused by both BL and parasite density while the accumulated erythrocytic pyrimidine nucleotides are caused by inhibition of pyrimidine 5 nucleotidase enzyme (P5N) by BL. Enhanced oxidative challenges brought about by both parasites and Lead ions induces eryptosis, while the accumulated nucleotides cause cellular hemolysis. The study on oxidative challenges and pyrimidine nucleotide levels during both malaria infection and Lead toxicity was outside the scope of this work. Considering the group of 39 participants (Table 1) with parasite density (PD) (1.2-2.2×10 3 parasites/µL of blood), no detectible BLL, perfectly functioning heme biosynthesis system FEP ˃10µg/ dL, and no anemia (mean Hb=10.4 g/ dL). It is likely that low PD by its own may not induce anemia especially to people living in malaria endemic areas. However, coexistence with another confounding factor like BL heightens the anemia pathogenesis. We can therefore hypothesize that progression to a severe anemia status is multifactorial and is exacerbated by having both malaria infection and blood Lead simultaneously. Table-1, further shows a 4% (n=8) severe anemic group of participants (Hb < 5g/dL), with a fairly functioning heme synthesis system (FEP = 36.2 µg/ dL) still under 50µg/ dL but with elevated PD. This gives an indication that the rate at which red blood cells (RBCs) were being destroyed by parasites surpassed their rate of synthesis hence the observed severe anemia status. Indiscriminate destruction of RBCs cause imbalances in the mediators of inflammation, Interleukin 6 cytokines (IL6) levels and this may affect the hepcidin expression [32, 33]. Interferon (IFN)-γ) known to induce the production of TRAIL (TNF-related apoptosis-inducing ligand) is also activated by elevated PD (34). Again, the reported BLL (9.7 µg/ dL) of this group was high enough to induce oxidative stress. As well as inhibition of P5N enzyme hence eryptosis and hemolysis respectively, likely associated with the observed low Hb status. It is further observed (Table 1) that 16/198 participants that had low PD and elevated BLL were anemic (Hb=7.0g/dL) and suffered from iron deficiency anemia as indicated by the heme biosynthesis disorder (FEP ˃50µg/ dL). The group’s low mean Hb levels could be attributed to elevated PD, BLL and other cofounding factors like iron deficiency due to malnutrition, inhibited iron absorption and inhibited (δ-ALAD) enzyme activity. This study reports a lower mean BLL (=8.6µg/dL) compared to literature [35-37]. Furthermore, an uneven distribution of BLL among the study participants was observed. This is attributed to the fact that BL binds the RBC’s enzyme δ-ALAD which is polymorphic with two major alleles; δ-ALAD-1 and δ-ALAD-2 that differ in electronegativity [38]. Having high frequency of δ-ALAD-1 translates into higher BL burden than those with δ-ALAD-2 [39]. A moderate interaction between the three variables (FEP/Hb, Parasite density and hemoglobin levels) after a multivariate analysis (Table 2) is reported. In addition, parasite density and BLL significantly correlated (r =0.377, P = ˂0.001) to affect the anemia status of the host Table 2. Regression analysis (Pearson’s) models show that FEP: Hb µg /g ratio increases exponentially with the blood Pb while Hb decreased with increasing FEP (Fig. 1). Again, a negative and strong Pearson correlation between FEP: Hb µg /g and Hb levels (Table 2 and Fig.1) as compared to that of parasite density and hemoglobin levels (Fig.2) were observed. This seem to concur with the argument that the etiology of severe anemia is multifactorial and therefore, there is need to study each of these contributing factors. It can therefore be speculated that progression to severe anemia during malaria infection and Lead toxicity involves destruction of erythrocytes, inhibition of heme synthesis inhibition (ineffective erythropoiesis) and interference of hepcidin iron regulatory system. The increased erythrocytic clearance due to extrinsic and intrinsic challenges increase the susceptibility to phagocytosis and hence anemia. Reduced serum iron (substrate) availability further complicates the heme synthesis mechanism by disturbing the enzyme /substrate enzymatic reaction equation. However, this study did not find a direct relationship between high blood Lead concentrations with parasite density as previously reported. It is likely that as BLL increases, hepcidin expression is upregulated decreasing the available serum iron important for parasite survival. Blood Lead levels and malaria parasites (Table 2 and Fig.3) in the study population seems to support the argument that BLL had little or no direct effect on parasite density levels. Children who participated in the study had no co-morbid diseases like HIV, sickle cell traits which are known confounding factors of anemia pathogenesis. This study therefore represents the first reported significant association between malaria and Lead poisoning during the anemia pathogenesis among the pediatric population in Uganda. Study limitations This study did not asses the nutritional status of the participants which is an important confounding factor. We therefore recommend that future studies incorporate nutritional status of participants for a better understanding of the relationship between blood Lead levels and anemia during malaria infection. Conclusion Based on this study findings, low blood Lead is a key confounding factor of anemia pathogenesis especially in children infected with malaria. Since both blood Lead and plasmodium infection geographically overlap with similar hematological consequences, their co–existence heightens the anemia status of the host. We recommend a detailed study involving a bigger sample size to properly understand the effects and anemia pathogenesis during Lead exposure and Plasmodium malaria infection over a long period of time. Abbreviations ALA: Aminolevulinic acid ALAD: Aminolevulinic acid dehydrogenase BL: Blood lead BLL: Blood lead levels FEP: Free erythrocyte protoporphyrin PD: Parasite density PPN: Protoporphyrin ZPP: Zinc protoporphyrin Declarations Ethics approval and consent to participate This study was approved by Gulu University Research Ethics Committee No. (GUREC-048). Parents/guardians were duly explained in a local language, the purpose and benefit of the study to their community and signed consent forms on behalf of their children. Consent for publication Not Applicable. Availability of data and materials The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request. Competing interests No competing interests in this study. Funding Not applicable. Authors’ contributions AM conceived the study idea, participated in data acquisition and manuscript preparation; DK participated in data analysis and manuscript preparation; CA participated in data acquisition and analysis; PCV participated in study design and data acquisition; JK participated in the study design, data interpretation, manuscript revision and submission. All authors have read and approved the manuscript for publication. Acknowledgements Acknowledgement goes to all those that participated in this study, most especially the children and parents/guardians of Katanga, Kampala, Uganda. Special thanks go to Mr. Paul Ojambo and Mr. Geofrey Mugenyi of the Department of biochemistry, Makerere University for their invaluable laboratory technical support to this study. References SLUMS IN UGANDA, The case of Katanga Slum in Kawempe division, Kampala district Uganda. https://www.academia.edu/.../SLUMS_IN_UGANDA_The_case_of_Katanga_Slum_ Worrall, E., Basu, S., & Hanson, K. (2005). Is malaria a disease of poverty? A review of the literature. Tropical Medicine & International Health , 10 (10), 1047-1059. Mghweno, L. R., Makokha, A. O., Magoha, H. S., Wekesa, J. M., & Nakajugo, A. (2008). Environmental lead pollution and food safety around Kampala City in Uganda. J Applied Biosci , 12 , 642-649. Idro, R., Aloyo, J., Mayende, L., Bitarakwate, E., John, C. C., & Kivumbi, G. W. (2006). Severe malaria in children in areas with low, moderate and high transmission intensity in Uganda. Tropical Medicine & International Health, 11(1), 115-124 Kamau, A., Mtanje, G., Mataza, C., Mwambingu, G., Mturi, N., Mohammed, S., & Snow, R. W. (2020). Malaria infection, disease and mortality among children and adults on the coast of Kenya. Malaria journal , 19 (1), 1-12. Yeka, A., Gasasira, A., Mpimbaza, A., Achan, J., Nankabirwa, J., Nsobya, S., Staedke, S. G., Donnelly, M. J., Wabwire-Mangen, F., Talisuna, A., Dorsey, G., Kamya, M. R., & Rosenthal, P. J. (2012). Malaria in Uganda: challenges to control on the long road to elimination: I. Epidemiology and current control efforts. Acta tropica , 121 (3), 184–195. https://doi.org/10.1016/j.actatropica.2011.03.004 Mpimbaza A, Ndeezi G, Katahoire A, Rosenthal PJ, Karamagi C. Demographic, Socioeconomic, and Geographic Factors Leading to Severe Malaria and Delayed Care Seeking in Ugandan Children: A Case-Control Study. The American Journal of Tropical Medicine and Hygiene. 2017 Nov; 97(5):1513-1523. DOI: 10.4269/ajtmh.17-0056. Green, H. K., Sousa-Figueiredo, J. C., Basanez, M. G., Betson, M., Kabatereine, N. B., Fenwick, A., & Stothard, J. R. (2011). Anaemia in Ugandan preschool-aged children: the relative contribution of intestinal parasites and malaria. Parasitology , 138 (12), 1534-1545. Yeka, A., Nankabirwa, J., Mpimbaza, A., Kigozi, R., Arinaitwe, E., Drakeley, C., & Staedke, S. G. (2015). Factors associated with malaria parasitemia, anemia and serological responses in a spectrum of epidemiological settings in Uganda. PLoS One , 10 (3), e0118901. La-Llave-León, O., Méndez-Hernández, E. M., Castellanos-Juárez, F. X., Esquivel-Rodríguez, E., Vázquez-Alaniz, F., Sandoval-Carrillo, A., & Salas-Pacheco, J. M. (2017). Association between blood lead levels and delta-aminolevulinic acid dehydratase in pregnant women. International journal of environmental research and public health , 14 (4), 432. Bergdahl, I. A., Grubb, A., Schütz, A., Desnick, R. J., Wetmur, J. G., Sassa, S., & Skerfving, S. (1997). Lead binding to δ‐aminolevulinic acid dehydratase (ALAD) in human erythrocytes. Pharmacology & toxicology, 81(4), 153-158 Schwartz, J., Landrigan, P. J., Baker Jr, E. L., Orenstein, W. A., & Von Lindern, I. H. (1990). Lead-induced anemia: dose-response relationships and evidence for a threshold. American journal of public health , 80 (2), 165-168. 13. Apostoli, P., Romeo, L., De Matteis, M. C., Menegazzi, M., Faggionato, G., & Vettore, L. (1988). Effects of lead on red blood cell membrane proteins. International archives of occupational and environmental health , 61 (1-2), 71-75. Oskarsson, A., & Fowler, B. A. (1985). Effects of lead on the heme biosynthetic pathway in rat kidney. Experimental and Molecular Pathology , 43 (3), 409-417. Sassa, S. (1978). Toxic effects of lead, with particular reference to porphyrin and heme metabolism. In Heme and hemoproteins (pp. 333-371). Springer, Berlin, Heidelberg. Marsh Jr, W. L., Nelson, D. P., & Koenig, H. M. (1983). Free erythrocyte protoporphyrin (FEP) I. Normal values for adults and evaluation of the hematofluorometer. American journal of clinical pathology , 79 (6), 655-660. Osterbauer, B., Kapisi, J., Bigira, V., Mwangwa, F., Kinara, S., Kamya, M. R., & Dorsey, G. (2012). Factors associated with malaria parasitaemia, malnutrition, and anaemia among HIV-exposed and unexposed Ugandan infants: a cross-sectional survey. Malaria journal, 11(1), 1-6. Rahman, M. S., Mushfiquee, M., Masud, M. S., & Howlader, T. (2019). Association between malnutrition and anemia in under-five children and women of reproductive age: Evidence from Bangladesh Demographic and Health Survey 2011. PloS one, 14(7). Wasserman, G., Graziano, J. H., Factor-Litvak, P., Popovac, D., Morina, N., Musabegovic, A., & Hadzialjevic, S. (1992). Independent effects of Lead exposure and iron deficiency anemia on developmental outcome at age 2 years. The Journal of pediatrics , 121 (5), 695-703. Wang, L., Johnson, E. E., Shi, H. N., Walker, W. A., Wessling-Resnick, M., & Cherayil, B. J. (2008). Attenuated inflammatory responses in hemochromatosis reveal a role for iron in the regulation of macrophage cytokine translation. The Journal of Immunology , 181 (4), 2723-2731 Mahaffey, K. R., & Annest, J. L. (1986). Association of erythrocyte protoporphyrin with blood Lead level and iron status in the Second National Health and Nutrition Examination Survey, 1976–1980. Environmental Research, 41(1), 327-338. Verschoor, M., Herber, R., Zielhuis, R., & Wibowo, A. (1987). Zinc protoporphyrin as an indicator of lead exposure: precision of zinc protoporphyrin measurements. International archives of occupational and environmental health , 59 (6), 613-621. Ghosh, K., & Ghosh, K. (2007). Pathogenesis of anemia in malaria: a concise review. Parasitology research , 101 (6), 1463-1469. Oppenheimer, S. J. (1989). Iron and infection: the clinical evidence. Acta Paediatrica , 78 , 53-62. Levander, O. A. (1979). Lead toxicity and nutritional deficiencies. Environmental health perspectives , 29 , 115-125. Flora, G., Gupta, D., & Tiwari, A. (2012). Toxicity of Lead: a review with recent updates. Interdisciplinary toxicology, 5(2), 47-58. Trape, J. F. (1985). Rapid evaluation of malaria parasite density and standardization of thick smear examination for epidemiological investigations. Transactions of the Royal Society of Tropical Medicine and Hygiene, 79(2), 181- Navarro, J. A., Granadillo, V. A., Parra, O. E., & Romero, R. A. (1989). Determination of lead in whole blood by graphite furnace atomic absorption spectrometry with matrix modification. Journal of Analytical Atomic Spectrometry, 4(5), 401-406. Piomelli, S. (1973). A micro method for free erythrocyte porphyrins: the FEP test. The Journal of laboratory and clinical medicine, 81(6), 932-940. Balasubramaniam, P., & Malathi, A. (1992). Comparative study of hemoglobin estimated by Drabkin's and Sahli's methods. Journal of postgraduate medicine, 38(1), 8. Wright, R. O., Tsaih, S. W., Schwartz, J., Wright, R. J., & Hu, H. (2003). Association between iron deficiency and blood lead level in a longitudinal analysis of children followed in an urban primary care clinic. The Journal of pediatrics, 142(1), 9-14. Cheria[1] n, S., Forbes, D. A., Cook, A. G., Sanfilippo, F. M., Kemna, E. H., Swinkels, D. W., & Burgner, D. P. (2008). An insight into the relationships between hepcidin, anemia, infections and inflammatory cytokines in pediatric refugees: a cross-sectional study. PloS one , 3 (12), e4030. Prakash, D., Fesel, C., Jain, R., Cazenave, P. A., Mishra, G. C., & Pied, S. (2006). Clusters of cytokines determine malaria severity in Plasmodium falciparum–infected patients from endemic areas of Central India. The Journal of infectious diseases , 194 (2), 198-207. Liu, M., Dickinson-Copeland, C., Hassana, S., & Stiles, J. K. (2016). Plasmodium-infected erythrocytes (pRBC) induce endothelial cell apoptosis via a heme-mediated signaling pathway. Drug design, development and therapy , 10 , 1009. Cusick, S. E., Jaramillo, E. G., Moody, E. C., Ssemata, A. S., Bitwayi, D., Lund, T. C., & Mupere, E. (2018). Assessment of blood levels of heavy metals including Lead and manganese in healthy children living in the Katanga settlement of Kampala, Uganda. BMC public health, 18(1), 717. Nabulo, G., Oryem-Origa, H., & Diamond, M. (2006). Assessment of lead, cadmium, and zinc contamination of roadside soils, surface films, and vegetables in Kampala City, Uganda. Environmental Research, 101(1), 42-52. Graber, L. K., Asher, D., Anandaraja, N., Bopp, R. F., Merrill, K., Cullen, M. R. & Trasande, L. (2010). Childhood lead exposure after the phaseout of Leaded gasoline: an ecological study of school-age children in Kampala, Uganda. Environmental health perspectives, 118(6), 884-889. Shaik, A. P., & Jamil, K. (2008). A study on the ALAD gene polymorphisms associated with lead exposure. Toxicology and industrial health , 24 (7), 501-506. da Cunha Martins Jr, A., Mazzaron Barcelos, G. R., Jacob Ferreira, A. L. B., de Souza, M. F., de Syllos Cólus, I. M., Greggi Antunes, L. M., ... & Barbosa Jr, F. (2015). Effects of lead exposure and genetic polymorphisms on ALAD and GPx activities in Brazilian battery workers. Journal of Toxicology and Environmental Health, Part A , 78 (16), 1073-1081. Supplementary Files Formulas.pdf Formulas 1 - 3 Cite Share Download PDF Status: Published Journal Publication published 14 Nov, 2020 Read the published version in BMC Pediatrics → Version 3 posted Submission checks completed at journal 30 Oct, 2020 Editorial decision: Accept 30 Oct, 2020 You are reading this latest preprint version Show more versions Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-52787","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research article","associatedPublications":[],"authors":[{"id":4147152,"identity":"acd7ce4d-e716-431b-9ef6-d166481a2574","order_by":0,"name":"Ambrose K Mukisa","email":"","orcid":"https://orcid.org/0000-0002-6570-1102","institution":"Makerere University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Ambrose","middleName":"K","lastName":"Mukisa","suffix":""},{"id":4147153,"identity":"2d1de2ad-2d3f-4342-a129-1b8006ca440c","order_by":1,"name":"Denis M Kasozi","email":"","orcid":"","institution":"Makerere University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Denis","middleName":"M","lastName":"Kasozi","suffix":""},{"id":4147154,"identity":"b66a73e0-b28b-4fea-92da-0b20b7f99339","order_by":2,"name":"Claire Aguttu","email":"","orcid":"","institution":"Makerere University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Claire","middleName":"","lastName":"Aguttu","suffix":""},{"id":4147155,"identity":"e4b706e0-1d1d-4b9c-bce0-8b0fbf894b28","order_by":3,"name":"Peter C. Vuzi","email":"","orcid":"","institution":"Makerere University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Peter","middleName":"C.","lastName":"Vuzi","suffix":""},{"id":4147156,"identity":"bb5c2dc2-872e-4f95-b2df-883ea4b82f7d","order_by":4,"name":"Joseph Kyambadde","email":"data:image/png;base64,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","orcid":"https://orcid.org/0000-0001-9128-2063","institution":"Makerere University","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Joseph","middleName":"","lastName":"Kyambadde","suffix":""}],"badges":[],"createdAt":"2020-08-03 10:37:44","currentVersionCode":3,"declarations":"","doi":"10.21203/rs.3.rs-52787/v3","doiUrl":"https://doi.org/10.21203/rs.3.rs-52787/v3","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1186/s12887-020-02412-2","type":"published","date":"2020-11-14T15:02:09+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":3382543,"identity":"45a132f5-031b-4a73-bd02-9ad77d110e7b","added_by":"auto","created_at":"2020-11-04 19:49:30","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":157586,"visible":true,"origin":"","legend":"Scatter graph showing how FEP/Hb levels relate with hemoglobin levels of malaria infected children. ","description":"","filename":"F1.png","url":"https://assets-eu.researchsquare.com/files/rs-52787/v3/68fed082551e110cff1f13fc.png"},{"id":3382545,"identity":"ab40abe4-fae3-403c-a6df-ca805e207954","added_by":"auto","created_at":"2020-11-04 19:49:30","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":58436,"visible":true,"origin":"","legend":"Scatter graph showing relationship between parasite density and hemoglobin levels among malaria infected children ","description":"","filename":"F2.png","url":"https://assets-eu.researchsquare.com/files/rs-52787/v3/31605ccc0fb2e093410fdacb.png"},{"id":3382546,"identity":"047999e8-f6bc-4b1a-97ae-cc9e6305ac5c","added_by":"auto","created_at":"2020-11-04 19:49:31","extension":"png","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":77948,"visible":true,"origin":"","legend":"Scatter graph showing relationship between Blood Lead levels and hemoglobin levels among malaria infected children ","description":"","filename":"F3.png","url":"https://assets-eu.researchsquare.com/files/rs-52787/v3/3cccb0a0b827b84bd8cf7ed6.png"},{"id":13609330,"identity":"b2e205e3-afcf-4a60-9166-ccfb9e5e81f9","added_by":"auto","created_at":"2021-09-17 06:19:52","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":648085,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-52787/v3/1522805b-052e-44ac-bf98-7804f7ae7259.pdf"},{"id":3382544,"identity":"e59e28c0-70c1-47a0-aa83-cc903430e35b","added_by":"auto","created_at":"2020-11-04 19:49:30","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":36467,"visible":true,"origin":"","legend":"Formulas 1 - 3","description":"","filename":"Formulas.pdf","url":"https://assets-eu.researchsquare.com/files/rs-52787/v3/a82a417e11a8d05d421804ca.pdf"}],"financialInterests":"","formattedTitle":"\u003cp\u003eRelationship between blood Lead status and anemia in Ugandan children with malaria infection\u003c/p\u003e","fulltext":[{"header":"Background","content":"\u003cp\u003eLike many malaria holoendemic developing countries, Uganda is faced with health-threatening diseases including anemia. Majority (60%) of the Uganda\u0026rsquo;s urban poor live in social disadvantaged slums like Katanga, Kampala [1, 2]. In such areas people live next to industries, workshops, motor garages, metal crafts yards, battery recycling plants, mosquito breeding grounds, and landfills predisposing them to frequent malaria infections as well as Lead intoxication. Several studies report elevated environmental Lead levels in water sources, soils, foodstuffs and air around Kampala city [3].\u003c/p\u003e\n\u003cp\u003eMalaria and Lead pollution geographically overlap, with water acting as sinks to Lead contaminated runoffs as well as bleeding ground for mosquitos. According to a study by [4], malaria infection accounts for up to 40% of all hospital outpatient visits, 25% of all hospital admissions, and 14% of all hospital deaths [4, 5] despite government efforts to curb its transmission [6]. Already fifty-three percent of children under the age of 5 years in Katanga area are reported to be anemic [6-9] this being attributed to high malaria prevalence.\u003c/p\u003e\n\u003cp\u003eAlthough, malaria infections remain the key cause of anemia in Uganda, other neglected yet significant confounders contributing to its pathogenesis include Lead poisoning, nutritional status, and helminths among others [6-9]. Lead exposure accounts for an individual\u0026rsquo;s blood Lead level (BLL) and is more evident in developing fetus where it directly affects the hematopoietic system. Ninety-nine percent of all the BL sink in the erythrocytes and 80% of it bind the \u0026delta;-aminolevulinic acid dehydratase (\u0026delta;-ALAD) enzyme. This enzyme is believed to catalyze the formation of porphobilinogen from \u0026delta;-aminolevulinic acid (ALA) [10,]. \u0026nbsp;During Lead intoxication, BL inhibits activity(s) of ALAD and limit the transfer of iron from endosomes to the cytoplasm resulting into fragile cell membrane which in turn shorten the lifespan of the circulating erythrocytes [11-13].\u003c/p\u003e\n\u003cp\u003eBlood Lead further triggers a reduction in red blood cells (RBC) production by specifically inhibiting Ferrochelatase a mitochondrial enzyme that catalyzes the insertion of iron into protoporphyrin during heme formation. Aminolevulinic acid synthetase (ALAS) a mitochondrial enzyme that catalyzes the formation of aminolevulinic acid (ALA) from succinyl CoA and glycine is also affected by BL [13-16]. In a normal heme synthesis system, the rate of iron formation and utilization is well balanced, however, this equilibrium is disturbed with insufficiency of iron. \u0026nbsp;\u003c/p\u003e\n\u003cp\u003eHigh malaria burden, overproduction of protoporphyrin, iron deficiency, inhibited ALAD and impaired ferro chelatase activity accounts for anemia pathogenesis. During malaria infection, intrinsic and extrinsic challenges are elevated and they induce iron deficiency anemia whose persistence elevates the rate at which Lead is absorbed [17-19]. In addition to other symptoms, malaria infection becomes fatal with severe anemia. Coupled with severe anemia, malaria is a result of massive erythrocyte\u003cspan style=\"text-decoration: line-through;\"\u003es\u003c/span\u003e lysis because of raising parasite density which in turn causes rupture of parasitized and non-parasitized red cells. Its persistence activates the splenic and other macrophages activities for phagocytosis [20] resulting into severe anemia.\u003c/p\u003e\n\u003cp\u003eReduced cellular iron concentrations on the other hand enhances Lead\u0026rsquo;s effects on major synthesis reactions. For example, ferrochelatase enzyme which is specifically sensitive to low iron levels is affected by lead toxicity (21). Also zinc instead of iron is incorporated into protoporphyrin (PPN) resulting in elevated levels of zinc protoporphyrin (ZPP) [22]. Zinc protoporphyrin or FEP concentrations are key biomarkers of heme synthesis status, and therefore, their elevation in concentration is associated with iron deficiency due to heme synthesis disorders [23, 24].\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;Since red cells are at the centre of anemia pathogenesis, as well as home to both the malaria parasites and BL, their co-existence propagates the host cell\u0026rsquo;s survival challenges.\u0026nbsp; The severity of anemia is dependent on the host's age and nutrition status [25, 26]. In addition, the combined aftermath of malaria parasites and BL among individuals living in malaria-endemic regions heighten the progression to severe anemia. This study therefore aimed at expounding the anemia status of these Ugandan children who are exposed to both malaria infection and environmental lead pollution. In this study, hemoglobin (Hb) concentration was used as a measure of one\u0026rsquo;s anemia status, and FEP/Hb ratio for heme synthesis status.\u003c/p\u003e"},{"header":"Methods","content":"\u003cp\u003eThis was a cross-sectional study on children aged 6 -60months living in Katanga area- Kampala city. The children were first screened for Plasmodium parasite infection before recruitment using rapid diagnostic kits. Children with malaria negative test, HIV positive test, blood transfusion history and signs of malnutrition were excluded from the study. Five (5) mls of venous blood from 198 malaria positive children were collected into EDTA by Qualified Nurses and Technicians. The samples were then transported on ice to Makerere University, Biochemistry Department L\u003cspan style=\"text-decoration: line-through;\"\u003ea\u003c/span\u003eboratory, stratified according to malaria parasite density and kept at 4\u003csup\u003e0\u003c/sup\u003eC awaiting various analysis and determinations.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetermination of Parasite density by thick smear method\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThick smears were prepared as described by [27], air dried, stained with 10% Leishman without fixing and examined under a CX 21 Olympus microscope. Five hundred leukocytes plus the number of malaria parasites seen in the same field were recorded. The number of malaria parasites per microliter (parasite/\u0026micro;L) of blood was expressed as the reciprocal of the mean counts in the three slides divided by the leukocyte counts, multiplied by a factor of 8000 i.e.\u003c/p\u003e\n\u003cp\u003e\u003cimg style=\"width: 555px;\" 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\" alt=\"\" /\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetermination of blood Lead levels by atomic absorption spectrometry\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eBlood Lead levels were determined following a method described by [28] using an atomic absorption spectrophotometer (Agilent 2000 series) equipped with a graphite tube atomizer and deuterium background correction facility. A hollow-cathode Lead lamp with a working current of 5mA, 283.3 nm spectral line and 0.5 nm bandwidth was used. Aliquots (500 \u0026micro;l) of whole blood melted with 1.2 ml of 0.5% Triton X-100 and 1% (NH\u003csub\u003e4\u003c/sub\u003e)\u003csub\u003e2\u003c/sub\u003eHPO\u003csub\u003e4\u003c/sub\u003e solution were added to 1.8 ml of deionized water and 1.5 ml of 20% Trichloroacetic acid (TCA) and vortex mixed. The samples were centrifuged for 20 min at 5000 rpm and 10 \u0026micro;l of the supernatant injected onto the graphite tube. The calibration curve was drawn using a standard addition method as described in M-572 of INSPQ\u0026rsquo;s Toxicology Laboratory method.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFluorospectrophotometric quantification of free erythrocyte protoporphyrins\u0026nbsp; \u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe FEP was measured following a method described by [29] using a fluorospectrophotometer set at 405nm excitation 610nm emissions.\u0026nbsp; The porphyrins were extracted by adding 20 \u0026micro;l aliquots of whole blood to a solution containing 100 \u0026micro;L of 10% ammonium sulfate and 5% celite and vortex mixed for 10sec.\u0026nbsp; 400 \u0026micro;l of 95% ethanol was then added and vortex mixed for more 20 seconds. This was followed by addition of 600 \u0026micro;l of acetone and further vortex mixing for 20 seconds. All the samples were put on an ice bath for 20 min, vortex mixed for 20 sec. and centrifuged at 4\u0026deg;C for 10 min. After which the supernatants were harvested into small borosilicate tubes and aliquots of 300 \u0026micro;l mixed with 300 \u0026micro;l of a solution containing propylene glycol and 1.5 N HCI, (4:1), let to stand for 20 min before reading at 405 nm excitation and 610 emissions. The FEP blood concentration was calculated using the following formulae;\u003c/p\u003e\n\u003cp\u003e\u003cimg style=\"width: 495px;\" 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\" alt=\"\" /\u003e\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eWhere Fz is the sample fluorescence, Cs\u0026nbsp;\u0026nbsp; concentration of the standard, Fs is the fluorescence of the standard, 2.7 is the final volume of HCl phase, 100 is the conversion factor to 100ml of extract, 1.1 conversion factor for protoporphyrin measured against a coproporphyrin standard and 0.2 (20\u0026micro;l) is the original blood volume measured.\u0026nbsp; The FEP/Hb ratio expressed as microgram Hb per gram Hb was calculated by dividing the FEP/dL RBC by gram Hb/dL.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eColorimetric determination of hemoglobin levels by blood cyanmethemoglobin reaction \u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eHemoglobin levels were determined by cyanmethemoglobin reaction method [30]. Aliquots 100\u0026micro;l of samples were made to a total volume of 1000\u0026micro;l with reaction solution containing 200mg of hexacyanoferrate III, 50mg of potassium cyanide, 140mg of potassium hydrogen phosphate and 1ml of Triton X-100 in a liter of distilled water. Then incubated for 15 min at room temperature before reading at 540 nm, with the blank being the reaction reagent. Then 500 \u0026micro;l of standard hemoglobin standard (0.7mg/ml) was diluted with 500 \u0026micro;l of the same reagent, treated as above and readings taken. The Hb concentration in g/dl was calculated using formula;\u0026nbsp; \u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cimg style=\"width: 488px;\" 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\" alt=\"\" /\u003e\u003c/p\u003e"},{"header":"Results","content":"\u003cp\u003eOf the 198 children enrolled in the study, 8/198 (4%) were severely anemic with \u0026lt; 5g/dL, mean parasite density =7.4\u0026times;10\u003csup\u003e3 \u003c/sup\u003eparasites/ \u0026micro;L, mean BLL =9.2\u0026plusmn;4.3 \u0026micro;g/dL, an FEP/Hb =7.4\u0026plusmn;2.9\u0026mu;g/g. Thirty nine (19.7%) of the study population \u0026nbsp;were not anemic with a mean value of Hb \u0026gt;10g Hb/dL (WHO cut off reference), mean parasite density =1.7\u0026times;10\u003csup\u003e3\u003c/sup\u003e parasites/ \u0026micro;L, mean BLL \u0026lt; 2\u0026micro;g/dL, Mean FEP/Hb =7.4\u0026plusmn;2.9 \u0026mu;g /g. The details of the distribution are shown in Table 1 below\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable.1 Distribution of parasite density, BLL, FEP/Hb and hemoglobin levels among 198 study participants.\u003c/strong\u003e\u003c/p\u003e\n\u003ctable border=\"1\" width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"21%\"\u003e\n\u003cp\u003eNo. of samples, N\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"22%\"\u003e\n\u003cp\u003eParasite density/ \u0026micro;L\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;(\u0026times;103)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"0%\"\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"23%\"\u003e\n\u003cp\u003eMean BLL (\u0026micro;g/dL)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"17%\"\u003e\n\u003cp\u003eMean FEP/Hb\u003c/p\u003e\n\u003cp\u003e(\u0026mu;g /g)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"13%\"\u003e\n\u003cp\u003eMean Hb\u003c/p\u003e\n\u003cp\u003e(g/ dL)\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"21%\"\u003e\n\u003cp\u003e16\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"22%\"\u003e\n\u003cp\u003e0 .1\u0026ndash;1.1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"0%\"\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"23%\"\u003e\n\u003cp\u003e10.3\u0026plusmn;1.9\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"17%\"\u003e\n\u003cp\u003e7.9\u0026plusmn;3\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"13%\"\u003e\n\u003cp\u003e7.0\u0026plusmn; 3.2\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"21%\"\u003e\n\u003cp\u003e39\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"22%\"\u003e\n\u003cp\u003e1.2\u0026ndash;2.2\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"0%\"\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"23%\"\u003e\n\u003cp\u003e\u0026lt; 2\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"17%\"\u003e\n\u003cp\u003e0.6\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"13%\"\u003e\n\u003cp\u003e10.4\u0026plusmn;2.7\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"21%\"\u003e\n\u003cp\u003e42\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"22%\"\u003e\n\u003cp\u003e2.3\u0026ndash;3.3\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"0%\"\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"23%\"\u003e\n\u003cp\u003e10.3\u0026plusmn;1.7\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"17%\"\u003e\n\u003cp\u003e2.9\u0026plusmn;3.2\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"13%\"\u003e\n\u003cp\u003e8.5\u0026plusmn;3.1\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"21%\"\u003e\n\u003cp\u003e35\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"22%\"\u003e\n\u003cp\u003e3.4\u0026ndash;4.4\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"0%\"\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"23%\"\u003e\n\u003cp\u003e7.9\u0026plusmn;2.1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"17%\"\u003e\n\u003cp\u003e3.9\u0026plusmn;2.8\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"13%\"\u003e\n\u003cp\u003e9.2\u0026plusmn;3.0\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"21%\"\u003e\n\u003cp\u003e34\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"22%\"\u003e\n\u003cp\u003e4.5\u0026ndash;5.5\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"0%\"\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"23%\"\u003e\n\u003cp\u003e7.9\u0026plusmn;1.8\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"17%\"\u003e\n\u003cp\u003e8.4\u0026plusmn;3.2\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"13%\"\u003e\n\u003cp\u003e9.3\u0026plusmn;4.1\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"21%\"\u003e\n\u003cp\u003e23\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"22%\"\u003e\n\u003cp\u003e5.6\u0026ndash;6.6\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"0%\"\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"23%\"\u003e\n\u003cp\u003e6.4\u0026plusmn;1.8\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"17%\"\u003e\n\u003cp\u003e4.9\u0026plusmn;2.7\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"13%\"\u003e\n\u003cp\u003e6.8\u0026plusmn;3.3\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"21%\"\u003e\n\u003cp\u003e8\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"22%\"\u003e\n\u003cp\u003e6.7\u0026ndash;7.7\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"0%\"\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"23%\"\u003e\n\u003cp\u003e9.7\u0026plusmn;5.2\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"17%\"\u003e\n\u003cp\u003e7.4\u0026plusmn;2.0\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"13%\"\u003e\n\u003cp\u003e4.9\u0026plusmn;2.9\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"21%\"\u003e\n\u003cp\u003e1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"22%\"\u003e\n\u003cp\u003e7.8\u0026ndash;8.8\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"0%\"\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"23%\"\u003e\n\u003cp\u003e5.1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"17%\"\u003e\n\u003cp\u003e5.5\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"13%\"\u003e\n\u003cp\u003e6.7\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"21%\"\u003e\n\u003cp\u003eN=198\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"22%\"\u003e\n\u003cp\u003eMean= 1.7\u0026times;10\u003csup\u003e3\u003c/sup\u003e\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"0%\"\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"23%\"\u003e\n\u003cp\u003eMean= 8.6\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"17%\"\u003e\n\u003cp\u003eMean= 4.4\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd width=\"13%\"\u003e\n\u003cp\u003eMean= 8.7\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003eHb=Hemoglobin, BLL = Blood Lead levels, FEP=Free erythrocyte protoporphyrin\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable 2. Correlation coefficients r and p-values of different interacting variables\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cimg 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\" alt=\"\" /\u003e\u003c/p\u003e"},{"header":"Discussion","content":"\u003cp\u003eSeveral cellular biomarkers are used to measure the extent and effects of blood Lead (BL) on various biochemical systems. \u0026nbsp;This study explored the levels of Free erythrocyte porphyrins (FEP) as a biomarker of heme biosynthesis disorder among the study participants. The heme biosynthesis process is inactivated by either iron deficiency, failed iron regulatory system or inhibited \u0026delta;-aminolevulinic acid dehydratase (\u0026delta;-ALAD) enzyme [31].\u003c/p\u003e\n\u003cp\u003eFree erythrocyte porphyrin levels of ˃50\u0026micro;g/ dL are important indicator of heme biosynthesis disorder. Common causes of this disorder include iron deficiency due to malnutrition and poor iron absorption, elevated hepcidin levels, and ALAD inhibition by blood Lead [16-18,20, 22, 29]. Findings of this study (Table 1) indicate that 88.2% (n=175) of all study participants were moderately anemic Hb ˃5g/dL \u0026lt; 10g/dL.\u0026nbsp; Of the 175 participants, 111 (56.6%) had normal functioning biosynthesis system (FEP levels ˃50\u0026micro;g/ dL), although with moderate anemia. Therefore, the observed low levels of Hb, cannot be associated with iron deficiency. The candid cofounding factors for the observed low Hb levels therefore are oxidative stress and accumulated erythrocytic pyrimidine nucleotides. \u0026nbsp;Oxidative may be caused by both BL and parasite density while the accumulated erythrocytic pyrimidine nucleotides are caused by inhibition of pyrimidine 5 nucleotidase enzyme (P5N) by BL. Enhanced oxidative challenges brought about by both parasites and Lead ions induces eryptosis, while the accumulated nucleotides cause cellular hemolysis. The study on oxidative challenges and pyrimidine nucleotide levels during both malaria infection and Lead toxicity was outside the scope of this work.\u003c/p\u003e\n\u003cp\u003eConsidering the group of 39 participants (Table 1) with parasite density (PD) (1.2-2.2\u0026times;10\u003csup\u003e3\u003c/sup\u003e parasites/\u0026micro;L of blood), no detectible BLL, perfectly functioning heme biosynthesis system FEP ˃10\u0026micro;g/ dL, and no anemia (mean Hb=10.4 g/ dL). It is likely that low PD by its own may not induce anemia especially to people living in malaria endemic areas. However, coexistence with another confounding factor like BL heightens the anemia pathogenesis. We can therefore hypothesize that progression to a severe anemia status is multifactorial and is exacerbated by having both malaria infection and blood Lead simultaneously.\u003c/p\u003e\n\u003cp\u003eTable-1, further shows a 4% (n=8) severe anemic group of participants (Hb \u0026lt; 5g/dL), with a fairly functioning heme synthesis system (FEP = 36.2 \u0026micro;g/ dL) still under 50\u0026micro;g/ dL but with elevated PD. This gives an indication that the rate at which red blood cells (RBCs) were being destroyed by parasites surpassed their rate of synthesis hence the observed severe anemia status. Indiscriminate destruction of RBCs cause imbalances in the mediators of inflammation, Interleukin 6 cytokines (IL6) levels and this may affect the hepcidin expression [32, 33]. Interferon (IFN)-\u0026gamma;) known to induce the production of TRAIL (TNF-related apoptosis-inducing ligand) is also activated by elevated PD (34). \u0026nbsp;Again, the reported BLL (9.7 \u0026micro;g/ dL) of this group was high enough to induce oxidative stress. As well as inhibition of P5N enzyme hence eryptosis and hemolysis respectively, likely associated with the observed low Hb status. It is further observed (Table 1) that 16/198 participants that had low PD and elevated BLL were anemic (Hb=7.0g/dL) and suffered from iron deficiency anemia as indicated by the heme biosynthesis disorder (FEP ˃50\u0026micro;g/ dL). The group\u0026rsquo;s low mean Hb levels could be attributed to elevated PD, BLL and other cofounding factors like iron deficiency due to malnutrition, inhibited iron absorption and inhibited (\u0026delta;-ALAD) enzyme activity.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eThis study reports a lower mean BLL (=8.6\u0026micro;g/dL) compared to literature [35-37]. \u0026nbsp;Furthermore, an uneven distribution of BLL among the study participants was observed. This is attributed to the fact that BL binds the RBC\u0026rsquo;s enzyme \u0026delta;-ALAD which is polymorphic with two major alleles; \u0026delta;-ALAD-1 and \u0026delta;-ALAD-2 that differ in electronegativity [38].\u0026nbsp; Having high frequency of \u0026delta;-ALAD-1 translates into higher BL burden than those with \u0026delta;-ALAD-2 [39].\u003c/p\u003e\n\u003cp\u003eA moderate interaction between the three variables (FEP/Hb, Parasite density and hemoglobin levels) after a multivariate analysis (Table 2) is reported. In addition, parasite density and BLL significantly correlated (r =0.377, P = ˂0.001) to affect the anemia status of the host Table 2. Regression analysis (Pearson\u0026rsquo;s) models show that FEP: Hb \u0026micro;g /g ratio increases exponentially with the blood Pb while Hb decreased with increasing FEP (Fig. 1). Again, a negative and strong Pearson correlation between FEP: Hb \u0026micro;g /g and Hb levels (Table 2 and Fig.1) as compared to that of parasite density and hemoglobin levels (Fig.2) were observed. This seem to concur with the argument that the etiology of severe anemia is multifactorial and therefore, there is need to study each of these contributing factors.\u003c/p\u003e\n\u003cp\u003eIt can therefore be speculated that progression to severe anemia during malaria infection and Lead toxicity involves destruction of erythrocytes, inhibition of heme synthesis inhibition (ineffective erythropoiesis) and interference of hepcidin iron regulatory system. The increased erythrocytic clearance due to extrinsic and intrinsic challenges increase the susceptibility to phagocytosis and hence anemia. Reduced serum iron (substrate) availability further complicates the heme synthesis mechanism by disturbing the enzyme /substrate enzymatic reaction equation.\u003c/p\u003e\n\u003cp\u003eHowever, this study did not find a direct relationship between high blood Lead concentrations with parasite density as previously reported. \u0026nbsp;It is likely that as BLL increases, hepcidin expression is upregulated decreasing the available serum iron important for parasite survival. Blood Lead levels and malaria parasites (Table 2 and Fig.3) in the study population seems to support the argument that BLL had little or no direct effect on parasite density levels.\u003c/p\u003e\n\u003cp\u003eChildren who participated in the study had no co-morbid diseases like HIV, sickle cell traits which are known confounding factors of anemia pathogenesis. This study therefore represents the first reported significant association between malaria and Lead poisoning during the anemia pathogenesis among the pediatric population in Uganda.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eStudy limitations\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThis study did not asses the nutritional status of the participants which is an important confounding factor. We therefore recommend that future studies incorporate nutritional status of participants for a better understanding of the relationship between blood Lead levels and anemia during malaria infection.\u003c/p\u003e"},{"header":"Conclusion","content":"\u003cp\u003eBased on this study findings, low blood Lead is a key confounding factor of anemia pathogenesis especially in children infected with malaria. Since both blood Lead and plasmodium infection geographically overlap with similar hematological consequences, their co\u0026ndash;existence heightens the anemia status of the host.\u003c/p\u003e\n\u003cp\u003eWe recommend a detailed study involving a bigger sample size to properly understand the effects and anemia pathogenesis during Lead exposure and Plasmodium malaria infection over a long period of time.\u003c/p\u003e"},{"header":"Abbreviations","content":"\u003cp\u003eALA:\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp; Aminolevulinic acid\u003c/p\u003e\n\u003cp\u003eALAD:\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp; Aminolevulinic acid dehydrogenase\u003c/p\u003e\n\u003cp\u003eBL:\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp; Blood lead\u003c/p\u003e\n\u003cp\u003eBLL:\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp; Blood lead levels\u003c/p\u003e\n\u003cp\u003eFEP:\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp; Free erythrocyte protoporphyrin\u003c/p\u003e\n\u003cp\u003ePD:\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp; Parasite density\u003c/p\u003e\n\u003cp\u003ePPN:\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp; Protoporphyrin\u003c/p\u003e\n\u003cp\u003eZPP:\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp; Zinc protoporphyrin\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eEthics approval and consent to participate\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThis study was approved by Gulu University Research Ethics Committee No. (GUREC-048). Parents/guardians were duly explained in a local language, the purpose and benefit of the study to their community and signed consent forms on behalf of their children.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConsent for publication\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNot Applicable.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAvailability of data and materials\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompeting interests\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNo competing interests in this study.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding \u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;Not applicable.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthors\u0026rsquo; contributions \u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAM conceived the study idea, participated in data acquisition and manuscript preparation; DK participated in data analysis and manuscript preparation; CA participated in data acquisition and analysis; PCV participated in study design and data acquisition; JK participated in the study design, data interpretation, manuscript revision and submission. All authors have read and approved the manuscript for publication.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAcknowledgements\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAcknowledgement goes to all those that participated in this study, most especially the children and parents/guardians of Katanga, Kampala, Uganda. Special thanks go to Mr. Paul Ojambo and Mr. Geofrey Mugenyi of the Department of biochemistry, Makerere University for their invaluable laboratory technical support to this study. \u0026nbsp;\u0026nbsp;\u0026nbsp;\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\n\u003cli\u003eSLUMS IN UGANDA, The case of Katanga Slum in Kawempe division, Kampala district Uganda. https://www.academia.edu/.../SLUMS_IN_UGANDA_The_case_of_Katanga_Slum_\u003c/li\u003e\n\u003cli\u003eWorrall, E., Basu, S., \u0026amp; Hanson, K. (2005). Is malaria a disease of poverty? A review of the literature.\u0026nbsp;\u003cem\u003eTropical Medicine \u0026amp; International Health\u003c/em\u003e,\u0026nbsp;\u003cem\u003e10\u003c/em\u003e(10), 1047-1059.\u003c/li\u003e\n\u003cli\u003eMghweno, L. R., Makokha, A. O., Magoha, H. S., Wekesa, J. M., \u0026amp; Nakajugo, A. (2008). Environmental lead pollution and food safety around Kampala City in Uganda.\u0026nbsp;\u003cem\u003eJ Applied Biosci\u003c/em\u003e,\u0026nbsp;\u003cem\u003e12\u003c/em\u003e, 642-649.\u003c/li\u003e\n\u003cli\u003eIdro, R., Aloyo, J., Mayende, L., Bitarakwate, E., John, C. C., \u0026amp; Kivumbi, G. W. (2006). Severe malaria in children in areas with low, moderate and high transmission intensity in Uganda. Tropical Medicine \u0026amp; International Health, 11(1), 115-124\u003c/li\u003e\n\u003cli\u003eKamau, A., Mtanje, G., Mataza, C., Mwambingu, G., Mturi, N., Mohammed, S., \u0026amp; Snow, R. W. (2020). Malaria infection, disease and mortality among children and adults on the coast of Kenya.\u0026nbsp;\u003cem\u003eMalaria journal\u003c/em\u003e,\u0026nbsp;\u003cem\u003e19\u003c/em\u003e(1), 1-12.\u003c/li\u003e\n\u003cli\u003eYeka, A., Gasasira, A., Mpimbaza, A., Achan, J., Nankabirwa, J., Nsobya, S., Staedke, S. G., Donnelly, M. J., Wabwire-Mangen, F., Talisuna, A., Dorsey, G., Kamya, M. R., \u0026amp; Rosenthal, P. J. (2012). Malaria in Uganda: challenges to control on the long road to elimination: I. Epidemiology and current control efforts.\u0026nbsp;\u003cem\u003eActa tropica\u003c/em\u003e,\u0026nbsp;\u003cem\u003e121\u003c/em\u003e(3), 184\u0026ndash;195. https://doi.org/10.1016/j.actatropica.2011.03.004\u003c/li\u003e\n\u003cli\u003eMpimbaza A, Ndeezi G, Katahoire A, Rosenthal PJ, Karamagi C. Demographic, Socioeconomic, and Geographic Factors Leading to Severe Malaria and Delayed Care Seeking in Ugandan Children: A Case-Control Study. The American Journal of Tropical Medicine and Hygiene. 2017 Nov; 97(5):1513-1523. DOI: 10.4269/ajtmh.17-0056.\u003c/li\u003e\n\u003cli\u003eGreen, H. K., Sousa-Figueiredo, J. C., Basanez, M. G., Betson, M., Kabatereine, N. B., Fenwick, A., \u0026amp; Stothard, J. R. (2011). Anaemia in Ugandan preschool-aged children: the relative contribution of intestinal parasites and malaria.\u0026nbsp;\u003cem\u003eParasitology\u003c/em\u003e,\u0026nbsp;\u003cem\u003e138\u003c/em\u003e(12), 1534-1545.\u003c/li\u003e\n\u003cli\u003eYeka, A., Nankabirwa, J., Mpimbaza, A., Kigozi, R., Arinaitwe, E., Drakeley, C., \u0026amp; Staedke, S. G. (2015). Factors associated with malaria parasitemia, anemia and serological responses in a spectrum of epidemiological settings in Uganda.\u0026nbsp;\u003cem\u003ePLoS One\u003c/em\u003e,\u0026nbsp;\u003cem\u003e10\u003c/em\u003e(3), e0118901.\u003c/li\u003e\n\u003cli\u003eLa-Llave-Le\u0026oacute;n, O., M\u0026eacute;ndez-Hern\u0026aacute;ndez, E. M., Castellanos-Ju\u0026aacute;rez, F. X., Esquivel-Rodr\u0026iacute;guez, E., V\u0026aacute;zquez-Alaniz, F., Sandoval-Carrillo, A., \u0026amp; Salas-Pacheco, J. M. (2017). Association between blood lead levels and delta-aminolevulinic acid dehydratase in pregnant women.\u0026nbsp;\u003cem\u003eInternational journal of environmental research and public health\u003c/em\u003e,\u0026nbsp;\u003cem\u003e14\u003c/em\u003e(4), 432.\u003c/li\u003e\n\u003cli\u003eBergdahl, I. A., Grubb, A., Sch\u0026uuml;tz, A., Desnick, R. J., Wetmur, J. G., Sassa, S., \u0026amp; Skerfving, S. (1997). Lead binding to \u0026delta;‐aminolevulinic acid dehydratase (ALAD) in human erythrocytes. Pharmacology \u0026amp; toxicology, 81(4), 153-158\u003c/li\u003e\n\u003cli\u003eSchwartz, J., Landrigan, P. J., Baker Jr, E. L., Orenstein, W. A., \u0026amp; Von Lindern, I. H. (1990). Lead-induced anemia: dose-response relationships and evidence for a threshold.\u0026nbsp;\u003cem\u003eAmerican journal of public health\u003c/em\u003e,\u0026nbsp;\u003cem\u003e80\u003c/em\u003e(2), 165-168.\u003c/li\u003e\n\u003cli\u003e13. Apostoli, P., Romeo, L., De Matteis, M. C., Menegazzi, M., Faggionato, G., \u0026amp; Vettore, L. (1988). Effects of lead on red blood cell membrane proteins.\u0026nbsp;\u003cem\u003eInternational archives of occupational and environmental health\u003c/em\u003e,\u0026nbsp;\u003cem\u003e61\u003c/em\u003e(1-2), 71-75.\u003c/li\u003e\n\u003cli\u003eOskarsson, A., \u0026amp; Fowler, B. A. (1985). Effects of lead on the heme biosynthetic pathway in rat kidney.\u0026nbsp;\u003cem\u003eExperimental and Molecular Pathology\u003c/em\u003e,\u0026nbsp;\u003cem\u003e43\u003c/em\u003e(3), 409-417.\u003c/li\u003e\n\u003cli\u003eSassa, S. (1978). Toxic effects of lead, with particular reference to porphyrin and heme metabolism. In Heme and hemoproteins (pp. 333-371). Springer, Berlin, Heidelberg.\u003c/li\u003e\n\u003cli\u003eMarsh Jr, W. L., Nelson, D. P., \u0026amp; Koenig, H. M. (1983). Free erythrocyte protoporphyrin (FEP) I. Normal values for adults and evaluation of the hematofluorometer.\u0026nbsp;\u003cem\u003eAmerican journal of clinical pathology\u003c/em\u003e,\u0026nbsp;\u003cem\u003e79\u003c/em\u003e(6), 655-660.\u003c/li\u003e\n\u003cli\u003eOsterbauer, B., Kapisi, J., Bigira, V., Mwangwa, F., Kinara, S., Kamya, M. R., \u0026amp; Dorsey, G. (2012). Factors associated with malaria parasitaemia, malnutrition, and anaemia among HIV-exposed and unexposed Ugandan infants: a cross-sectional survey. Malaria journal, 11(1), 1-6.\u003c/li\u003e\n\u003cli\u003eRahman, M. S., Mushfiquee, M., Masud, M. S., \u0026amp; Howlader, T. (2019). Association between malnutrition and anemia in under-five children and women of reproductive age: Evidence from Bangladesh Demographic and Health Survey 2011. PloS one, 14(7).\u003c/li\u003e\n\u003cli\u003eWasserman, G., Graziano, J. H., Factor-Litvak, P., Popovac, D., Morina, N., Musabegovic, A., \u0026amp; Hadzialjevic, S. (1992). Independent effects of Lead exposure and iron deficiency anemia on developmental outcome at age 2 years.\u0026nbsp;\u003cem\u003eThe Journal of pediatrics\u003c/em\u003e,\u0026nbsp;\u003cem\u003e121\u003c/em\u003e(5), 695-703.\u003c/li\u003e\n\u003cli\u003eWang, L., Johnson, E. E., Shi, H. N., Walker, W. A., Wessling-Resnick, M., \u0026amp; Cherayil, B. J. (2008). Attenuated inflammatory responses in hemochromatosis reveal a role for iron in the regulation of macrophage cytokine translation.\u0026nbsp;\u003cem\u003eThe Journal of Immunology\u003c/em\u003e,\u0026nbsp;\u003cem\u003e181\u003c/em\u003e(4), 2723-2731\u003c/li\u003e\n\u003cli\u003eMahaffey, K. R., \u0026amp; Annest, J. L. (1986). Association of erythrocyte protoporphyrin with blood Lead level and iron status in the Second National Health and Nutrition Examination Survey, 1976\u0026ndash;1980. Environmental Research, 41(1), 327-338.\u003c/li\u003e\n\u003cli\u003eVerschoor, M., Herber, R., Zielhuis, R., \u0026amp; Wibowo, A. (1987). Zinc protoporphyrin as an indicator of lead exposure: precision of zinc protoporphyrin measurements.\u0026nbsp;\u003cem\u003eInternational archives of occupational and environmental health\u003c/em\u003e,\u0026nbsp;\u003cem\u003e59\u003c/em\u003e(6), 613-621.\u003c/li\u003e\n\u003cli\u003eGhosh, K., \u0026amp; Ghosh, K. (2007). Pathogenesis of anemia in malaria: a concise review.\u0026nbsp;\u003cem\u003eParasitology research\u003c/em\u003e,\u0026nbsp;\u003cem\u003e101\u003c/em\u003e(6), 1463-1469.\u003c/li\u003e\n\u003cli\u003eOppenheimer, S. J. (1989). Iron and infection: the clinical evidence.\u0026nbsp;\u003cem\u003eActa Paediatrica\u003c/em\u003e,\u0026nbsp;\u003cem\u003e78\u003c/em\u003e, 53-62.\u003c/li\u003e\n\u003cli\u003eLevander, O. A. (1979). Lead toxicity and nutritional deficiencies.\u0026nbsp;\u003cem\u003eEnvironmental health perspectives\u003c/em\u003e,\u0026nbsp;\u003cem\u003e29\u003c/em\u003e, 115-125.\u003c/li\u003e\n\u003cli\u003eFlora, G., Gupta, D., \u0026amp; Tiwari, A. (2012). Toxicity of Lead: a review with recent updates. Interdisciplinary toxicology, 5(2), 47-58.\u003c/li\u003e\n\u003cli\u003eTrape, J. F. (1985). Rapid evaluation of malaria parasite density and standardization of thick smear examination for epidemiological investigations. Transactions of the Royal Society of Tropical Medicine and Hygiene, 79(2), 181-\u003c/li\u003e\n\u003cli\u003eNavarro, J. A., Granadillo, V. A., Parra, O. E., \u0026amp; Romero, R. A. (1989). Determination of lead in whole blood by graphite furnace atomic absorption spectrometry with matrix modification. Journal of Analytical Atomic Spectrometry, 4(5), 401-406.\u003c/li\u003e\n\u003cli\u003ePiomelli, S. (1973). A micro method for free erythrocyte porphyrins: the FEP test. The Journal of laboratory and clinical medicine, 81(6), 932-940.\u003c/li\u003e\n\u003cli\u003eBalasubramaniam, P., \u0026amp; Malathi, A. (1992). Comparative study of hemoglobin estimated by Drabkin's and Sahli's methods. Journal of postgraduate medicine, 38(1), 8.\u003c/li\u003e\n\u003cli\u003eWright, R. O., Tsaih, S. W., Schwartz, J., Wright, R. J., \u0026amp; Hu, H. (2003). Association between iron deficiency and blood lead level in a longitudinal analysis of children followed in an urban primary care clinic. The Journal of pediatrics, 142(1), 9-14.\u003c/li\u003e\n\u003cli\u003eCheria[1] n, S., Forbes, D. A., Cook, A. G., Sanfilippo, F. M., Kemna, E. H., Swinkels, D. W., \u0026amp; Burgner, D. P. (2008). An insight into the relationships between hepcidin, anemia, infections and inflammatory cytokines in pediatric refugees: a cross-sectional study.\u0026nbsp;\u003cem\u003ePloS one\u003c/em\u003e,\u0026nbsp;\u003cem\u003e3\u003c/em\u003e(12), e4030.\u003c/li\u003e\n\u003cli\u003ePrakash, D., Fesel, C., Jain, R., Cazenave, P. A., Mishra, G. C., \u0026amp; Pied, S. (2006). Clusters of cytokines determine malaria severity in Plasmodium falciparum\u0026ndash;infected patients from endemic areas of Central India.\u0026nbsp;\u003cem\u003eThe Journal of infectious diseases\u003c/em\u003e,\u0026nbsp;\u003cem\u003e194\u003c/em\u003e(2), 198-207.\u003c/li\u003e\n\u003cli\u003eLiu, M., Dickinson-Copeland, C., Hassana, S., \u0026amp; Stiles, J. K. (2016). Plasmodium-infected erythrocytes (pRBC) induce endothelial cell apoptosis via a heme-mediated signaling pathway.\u0026nbsp;\u003cem\u003eDrug design, development and therapy\u003c/em\u003e,\u0026nbsp;\u003cem\u003e10\u003c/em\u003e, 1009.\u003c/li\u003e\n\u003cli\u003eCusick, S. E., Jaramillo, E. G., Moody, E. C., Ssemata, A. S., Bitwayi, D., Lund, T. C., \u0026amp; Mupere, E. (2018). Assessment of blood levels of heavy metals including Lead and manganese in healthy children living in the Katanga settlement of Kampala, Uganda. BMC public health, 18(1), 717.\u003c/li\u003e\n\u003cli\u003eNabulo, G., Oryem-Origa, H., \u0026amp; Diamond, M. (2006). Assessment of lead, cadmium, and zinc contamination of roadside soils, surface films, and vegetables in Kampala City, Uganda. Environmental Research, 101(1), 42-52.\u003c/li\u003e\n\u003cli\u003eGraber, L. K., Asher, D., Anandaraja, N., Bopp, R. F., Merrill, K., Cullen, M. R. \u0026amp; Trasande, L. (2010). Childhood lead exposure after the phaseout of Leaded gasoline: an ecological study of school-age children in Kampala, Uganda. Environmental health perspectives, 118(6), 884-889.\u003c/li\u003e\n\u003cli\u003eShaik, A. P., \u0026amp; Jamil, K. (2008). A study on the ALAD gene polymorphisms associated with lead exposure.\u0026nbsp;\u003cem\u003eToxicology and industrial health\u003c/em\u003e,\u0026nbsp;\u003cem\u003e24\u003c/em\u003e(7), 501-506.\u003c/li\u003e\n\u003cli\u003eda Cunha Martins Jr, A., Mazzaron Barcelos, G. R., Jacob Ferreira, A. L. B., de Souza, M. F., de Syllos C\u0026oacute;lus, I. M., Greggi Antunes, L. M., ... \u0026amp; Barbosa Jr, F. (2015). Effects of lead exposure and genetic polymorphisms on ALAD and GPx activities in Brazilian battery workers.\u0026nbsp;\u003cem\u003eJournal of Toxicology and Environmental Health, Part A\u003c/em\u003e,\u0026nbsp;\u003cem\u003e78\u003c/em\u003e(16), 1073-1081.\u003c/li\u003e\n\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"bmc-pediatrics","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"bped","sideBox":"Learn more about [BMC Pediatrics](http://bmcpediatr.biomedcentral.com/)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/bped/default.aspx","title":"BMC Pediatrics","twitterHandle":"BMC_series","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"em","reportingPortfolio":"BMC Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Blood Lead, Malaria parasites, Anemia, Iron deficiency, Free erythrocyte protoporphyrins","lastPublishedDoi":"10.21203/rs.3.rs-52787/v3","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-52787/v3","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eBackground\u003c/strong\u003e\u003c/p\u003e\u003cp\u003eIn Uganda, childhood anemia remains a health challenge and is associated with malaria infection as well as iron deficiency. Iron deficiency is intertwined with nutritional status, age and other comorbidities including helminths and Lead toxicity. Environmental Lead levels accounts for one’s blood Lead (BL) levels. Blood Lead competitively blocks iron absorption, inhibits hemoglobin (Hb) biosynthesis and elevates free erythrocyte protoporphyrin (FEP) levels. Lead toxicity’s contribution towards anemia pathogenesis, especially during malaria infection has not been studied. Concomitant exposure to both malaria infection and Lead pollution, exacerbates the anemia status. This study therefore aimed at expounding the anemia status of these Ugandan children aged under 5years who are exposed to both malaria infection and environmental Lead pollution.\u003c/p\u003e\u003cp\u003e\u003cstrong\u003eMethods\u003c/strong\u003e\u003c/p\u003e\u003cp\u003eBriefly, venous blood samples from 198 children were microscopically assayed for malaria parasite density (PD), and hemoglobin (Hb) concentrations using the cyanmethemoglobin method, while BL and FEP levels were determined by the standard atomic absorption spectrophotometric and fluorometric methods respectively.\u003c/p\u003e\u003cp\u003e\u003cstrong\u003eResults\u003c/strong\u003e\u003c/p\u003e\u003cp\u003eOne hundred and fifty-one (76.3%) of the children analyzed had moderate anemia (Hb \u0026lt;10\u0026gt;5 g/dL) with Means of BLL=8.6 µg/dL, Hb =7.5 g/dL, FEP/Hb =8.3 µg/g and PD =3.21×103 parasites / µL, while eight (4%) were severely anemic (\u0026lt;5 g/dL). Regression analysis and statistical correlation between PD and Hb (r = -0.231, R\u003csup\u003e2\u003c/sup\u003e= 0.15 P-value \u0026lt; 0.001) was negative and weak as compared to that between FEP/Hb and Hb (r = -0.6, R\u003csup\u003e2\u003c/sup\u003e=0.572 P-value=0.001).\u003c/p\u003e\u003cp\u003e\u003cstrong\u003eConclusion\u003c/strong\u003e\u003c/p\u003e\u003cp\u003eBased on the study’s findings, we conclude that BL significantly contributes to the pathogenesis of anemia and therefore its co-existence with malaria infection in the host exacerbates the anemia status.\u003c/p\u003e\u003cp\u003e\u003cstrong\u003eTrial registration\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003eNot applicable\u003c/p\u003e","manuscriptTitle":"Relationship between blood Lead status and anemia in Ugandan children with malaria infection","msid":"","msnumber":"","nonDraftVersions":[{"code":3,"date":"2020-11-04 19:49:29","doi":"10.21203/rs.3.rs-52787/v3","editorialEvents":[{"type":"communityComments","content":0},{"type":"checksComplete","content":"","date":"2020-10-30T12:00:00+00:00","index":"","fulltext":""},{"type":"decision","content":"Accept","date":"2020-10-30T12:00:00+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"bmc-pediatrics","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"bped","sideBox":"Learn more about [BMC Pediatrics](http://bmcpediatr.biomedcentral.com/)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/bped/default.aspx","title":"BMC Pediatrics","twitterHandle":"BMC_series","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"em","reportingPortfolio":"BMC Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true}},{"code":2,"date":"2020-10-30 10:49:54","doi":"10.21203/rs.3.rs-52787/v2","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Minor revision","date":"2020-10-24T12:00:00+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2020-10-23T12:00:00+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2020-10-22T12:00:00+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2020-10-22T12:00:00+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"bmc-pediatrics","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"bped","sideBox":"Learn more about [BMC Pediatrics](http://bmcpediatr.biomedcentral.com/)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/bped/default.aspx","title":"BMC Pediatrics","twitterHandle":"BMC_series","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"em","reportingPortfolio":"BMC Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true}},{"code":1,"date":"2020-08-13 17:00:03","doi":"10.21203/rs.3.rs-52787/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Major revision","date":"2020-09-19T12:00:00+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2020-09-18T12:00:00+00:00","index":1,"fulltext":"Recommendation: Accept after minor essential revisions\nForm responses:\n---\n\nComments to Author:\n---\nPlease include all comments for the authors in this box rather than uploading your report as an attachment. Please only upload as attachments annotated versions of manuscripts, graphs, supporting materials or other aspects of your report which cannot be included in a text format.\n\nLet me commend the authors for doing a good job.\nMy general comment is that the manuscript has lots of grammatical errors which made the reading and proper interpretation of the manuscript a bit hectic. The authors have to correct these.\nThe abstract requires some corrections.\nThe Introduction: There are grammatical errors in this section that require correction also.\nMethods: The authors did not give the details of children who were excluded from the study. Authors only talked about blood malaria parasite positivity, meanwhile co-morbidities such as sickle cell anemia could affect the findings of the study and I think this and other confounders should the highlighted as exclusion criteria in the methods. It was mentioned towards the end of the discussion. Could the social class/ nutrition status of the children used in this study also have effect on their iron status maybe not necessarily because of lead poisoning alone? The anthropometric measurements and social classification could help shed more light on the findings.\nDiscussion: This section also has lots of grammatical errors. The authors should try and improve on the presentation of the discussion to make it clearer to the audience. FOA should be changed to FAO. Some portions of the attached manuscript are highlighted in green which the authors need to rewritten while the words in red should be deleted.\nThe authors need to improve on the grammar generally, hopefully then, the manuscript will be understood better.\n* Publons Reviewer Recognition. Springer Nature can send verification of this review directly to Publons (a subsidiary of Clarivate Analytics). If you would like to take advantage of this service, please click on the “Yes” option below. 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Please find the details of processing in Publons’ privacy policy https://publons.com/about/terms: **Yes**\n* Declaration of competing interests: **I declare that I have no competing interests.**\n* Reviewer Publication Consent. I agree for my report to be made available under an Open Access Creative Commons CC-BY License (http://creativecommons.org/licenses/by/4.0) if this manuscript is accepted for publication. Any comments that I do not wish to be included in the published report have been included as confidential comments to the editor, which will not be published.: **I agree to the terms of the CC-BY 4.0 license; please do not publish my name with my report. (default)**\n* Is the study design appropriate to answer the research question (including the use of appropriate controls), and are the conclusions supported by the evidence presented?: **Yes**\n* Are the methods sufficiently described to allow the study to be repeated?: **No**\n* Is the use of statistics and treatment of uncertainties appropriate?: **Yes**\n* Is the presentation of the work clear?: **No**\n* Are the images in this manuscript (including electrophoretic gels and blots) free from apparent manipulation?: **Yes**\n"},{"type":"editorInvitedReview","content":"","date":"2020-09-17T12:00:00+00:00","index":2,"fulltext":"Recommendation: Accept after minor essential revisions\nForm responses:\n---\n\nComments to Author:\n---\nReviews on Blood Lead alters the anemia status of malaria infected children in Katanga Kampala Uganda.\nDear Editor-in-chief,\nThank you for the privilege of reviewing this article. I have read through the article and below are my comments.\nGENERAL COMMENTS:\n1. This a good and publishable work but requires a few major and minor corrections.\n2. The \"L\" in Lead should be in capital throughout the text to maintain uniformity and to differentiate it from the English word \"lead\".\nTopic:\nCan be modified as \"Relationship between blood Lead status and anemia in Ugandan children with malaria infection.\nMethods:\nA major omission is that the authors failed to consider the nutritional status of this children which is a major contributor to anemia in children. Hence, the anthropometric measurements such as weight, height, mid arm circumference should be considered while interpreting the results.\nResults:\nTable1, ensure uniformity of decimal places. There is additional information in this table that would make the discussion more robust.\nDiscussion:\nThe mean BLL of those with higher parasitemia is similar to those with lower levels. Even lower than that recorded in some groups with lower parasitemia. Table1 showed that the mean BLL (9.7) in 8 children with severe anemia (Hb 4.9) is actually lower than the mean BLL in 82 children (10.4) with relatively higher Hb concentration despite having lower malaria parasite count. This suggests that the malaria infection rather than the blood Lead level could account for the severe anemia.\nAlso, a major confounder is the nutritional status of these children, which was not considered in the study. It will be good to know how the nutritional status of these children correlates with the degree of anemia as well. There is a need to state clearly the reason for this omission and include it as a limitation to the study findings.\nAlthough the study aimed to determine the contribution of BLL to anemia in children, are there other clinical symptoms of Lead poisoning identified in these children? If yes, it will be good to highlight some of them.\n\n\nPage 1\n\nLine 22/23, add \"In\" before \"Uganda\"\nLine 43, lead pollution, \"exacerbates\" not exerbates\n43-45, last sentence in the background could be rephrased as thus: \"This study therefore aimed at expounding the anaemia status of these Ugandan children who are exposed to both malaria infection and environmental lead pollution.\"\nLine 51, add comma (,) after \"Briefly\"\n\nPage 2\nLine 8-12, sentence can be rephrased thus; One hundred and fifty-one (76.3%) of the children analyzed had moderate anemia (Hb \u003c10\u003e5 g/dL) while eight (4%) had severe anemia (\u003c5 g/dL).\nLine 21-25, Basing on this study's findings, we conclude----- could be modified as \"Based on the study findings, we conclude that blood Lead is a significant contributor to the pathogenesis of anemia pathogenesis and therefore its co-existence with plasmodium malaria infection in the host exacerbates the anemia status.\"\nLine 34/35, Plasmodium malaria parasites. Note, Plasmodium malariae is a specie of plasmodium that causes malaria so writing \"Plasmodium malaria parasite\" may be confused as referring to this specie.\nLine 40 'Like many malaria \"halo endemic\" developing countries, should be \"holoendemic\"\nLine 52/53, Expunge \"Plasmodium\" as this imply a specie of plasmodium (Plasmodium malariae) which I presume isn't the message here. Sentence could be rephrased as \"According to the study done by (4), malaria accounts for----\nLine 56, \"53%\" should be written in words (Fifty-three percent) as it begins a sentence here.\n\nPage 3\nLine 6, delete \"are\" at the end of the line\nLine 8, \"confounders\" not cofounders. Are there other confounders considered e.g. Nutritional iron deficiency, hookworm infestation etc\nLine 19/20, Effect the changes in red\" ----of over 99% of the Lead present in the blood, and with over 80% delete is bound to δ-aminolevulinic acid \"\nLine 49/50, \"During malaria anemia, Lead becomes even more concentrated in red blood cells (Insert reference for this statement)\"\nLine 53-55, Expunge this sentence \"Malaria due to Plasmodium infections becomes fatal by causing severe anemia to the host though other symptoms may occur depending on the species\". Adds no value. Also, \"Malaria due to Plasmodium infections\" may be confused to mean malaria could be due to organisms other than plasmodium spp and there are other complications of malaria that can cause death, not only severe anemia.\nLine 58, \"parastemia\" correct spelling by adding \"I\" after \"s\" i.e parasitemia\n\nPage 4\nLine 59/60 page 3 and 6-8 page 4, recast sentence \"Because of increased parasite density in the host system, there are intrinsic and extrinsic changes to the red blood cells. This persistent situation in turn activates the splenic and other macrophages for phagocytosis [15] and resulting into anemia\". It can read thus: \"This occurs due to increased parasite density, which leads to intrinsic and extrinsic changes to the red blood cells. This in turn activates the splenic macrophages as well as other macrophages for phagocytosis [15] resulting in anemia.\"\nLine 22-26, recast as thus; \"Zinc protopophyrin or FEP concentrations have been used as a measure of heme synthesis inhibition. Increased levels are an indicator--------17\"\nLine 28/29, -----at the centre of anaemia----- \"c\" should be in small letter not capital.\nLine 56/57, Effect changes in red, thus; The samples were then kept at 40C while awaiting various analysis and determinations.\n\nPage 5\nProvide reference for determination of blood lead levels by atomic absorption spectrometry.\n\nPage 6\nProvide reference for colorimetric determination of hemoglobin levels by blood cyanmethemoglobin reaction\nLines 27/28 and 31/32, \"cyan methemoglobin\" should be written together as a compound word \"cyanmethemoglobin\"\n\nPage 7\nTable 1, ensure uniformity in decimal places\nLine 15/16, \"1\" is missing in the heading. i.e. Table 1\n\nPage 9\nFigure 3, Scatter graph showing how BLL (Blood lead levels) or BBL?------\n\nPage 9\nLine 42, BL and PD should be written in full first before abbreviation as they are appearing for the first time here in discussion i.e Blood Lead (BL) and parasite density (PD)\nLine47/48, Because the malaria parasites depends----- Delete \"s\".\nLine 53/54, ---this study, 80.3% had a mean BL level (9.3g/dL)---- This was not reported in table 1 or the prose before table 1. The mean BLL reported on page 6, Line 58 (mean BLL =9.2±4.3 μg/dL) is for severely anemic children. Therefore, kindly reflect it on the table or prose before using it in discussion.\nLine 55/56. Kindly rephrase the sentence \"There was a reduced parasite density (delete \"a\" before reduced and replace \"densities\" with \"density\") in samples with BL than those without suggesting that lead has a direct effect on the multiplication of the parasites\". This deduction cannot be made from the tables, prose or graphs presented.\n\nPage 10\nLine 8/9, please recast this sentence, \"Over accumulation of hepcidin reduces the available iron in circulation hence hard times for parasite's survival\". It could read as follows,\n\"Accumulation of hepcidin reduces the available iron in circulation, thus, making it hard for the parasite to survive.\" \nLine 17-21, Correct the grammar in the sentence \"This study found a relatively high malaria infection rate especially among children in this area as compared to one reported other parts of the city and this could as result of geographic and social disadvantages of the area (1).\"\nLine 21/22. \"A\" preceding majority should be expunged and \"can\" should be changed to \"may\" to read thus:\n\"Majority of the study participants are reported to have elevated blood Lead levels, and this may be attributed to….\nLine 37, Add full stop (.) after reference (24,25,26). Also, the reference can be written as (24-26)\nLine 38,39/40, What is the study hypothesis? The sentence below is not clear. It should be rephrased or expunged:\n\"The health burden of urban agriculture in Uganda has not been assessed, and this study hypothesizes that it could be the number one important source of dietary lead in children\"\nLine 41-44, Add reference to the sentence\" Because of the similarity in environmental risk factors, both malaria and BLL in children co-cluster within specific communities and households in urban areas of Uganda.\"\nLine 51/52, Add comma (,) after Kampala. The sentence \"We can speculate that a big percentage of urban Uganda dwellers ---- should be rephrased thus;\n\"We speculate that a high number of urban Uganda dwellers------\nLine 59, Expunge the sentence \"Results from this study shows that since enzymes are very sensitive in nature of their activity, any smallest amount of blood lead is enough to affect their sensitivity.\" It is a repetition of preceding sentence and may be confusing.\n\nPage 11,\nLine 5/6, ALAD should be in full as it begins a sentence, The abbreviation should then follow in bracket\nLine 10-17. The sentence \"Lead blocks affect ALAD, preventing iron incorporation step into protoporphyrin IX hence the accumulation of the free erythrocyte protoporphyrin …… study. \"Should be rephrased. Also, remove full stop (.) after protoporphyrin in Line 13/14 and \"Pb\" should be written as Lead.\nLine 17-19, Rephrase the sentence \"Since BLL initially accumulates in erythrocytes and has a high affinity for the -SH group of enzymes, d-ALAD enzyme is inhibited by blood Lead quickly falls victim to BLL even at very low levels.\" Also, is \"d-ALAD\" the same as \"ALAD\". Please maintain uniformity throughout text.\nLine 30-34. The sentence \"This phenomenon is attributed to the fact that blood lead mimics iron and hence fooling hepcidin mediated [30,]. Iron homeostasis that there is too much iron in circulation and if this persists, iron mobilization from the stores is shut down and leading to decreased iron availability for erythropoiesis.\" Remove comma (,) and full stop (.) after reference 30. \"I\" in iron should now be small letter.\nLine 37- 42/43, Rephrase the sentence \"Further to RBCs indiscriminate destruction by the malaria parasites, causing an imbalance in the cytokines levels and other mediators of inflammation such as hemozoin, a parasite-derived polymerized heme, and IL-6 one of the cytokines affected, has the capacity to affect the hepcidin expression (31).\"\nLine 44/45, TRAIL (TNF-related apoptosis inducing ligand). Should be written as \"TNF-related apoptosis inducing ligand (TRAIL)\"\nLine 46/47, the phrase ----\"splenic and other macrophages\" should be changed to ----\"splenic macrophages as well as other macrophages-----\"\n\nPage 12\nLine 9/10, to during Lead intoxication and hence------- delete \"to\"\nLine 15-19, Effect the corrections in red; \"There is moderate interaction between the three variables (FEP/Hb, Parasite density and hemoglobin levels) after a multivariate analysis (Table 2) supports our argument.\nLine 20/21- 22/23, Effect the corrections in red; ---\"in the study had no co-morbid diseases such as like HIV, sickle cell disease, traits which could have attenuated the relationships between malaria and BLL.\"\nLine 26, ----- BLL had a little or no direct effect on parasite------delete \"a\"\nLine 28/29, Effect the corrections in red; \"To the best of our knowledge, this study represents is the first to reported a significant association between----\nLine 40. \"Based on\" should replace 'basing on…'\nLine 47-51 \". We propose a detailed study involving a bigger sample size to properly understand of the effects and anemia pathogenesis during lead exposure of Plasmodium malaria infection over a long period of time.\" Kindly rephrase the sentence, it's confusing. You can look at the suggestion below and modify as appropriate,\n\"We propose a study involving a larger sample size to properly understand the effects of Lead exposure on anemia and malaria infection in children.\"\n\nPages 14-17\nReferences-\nPlease ensure you follow the recommended referencing style by BMC Pediatrics.\nKindly maintain uniformity in referencing- either write the journal names in full or use approved abbreviations by the cited journal.\nJournal articles usually have \"how to cite option\" to guide you.\nFollow appropriate method of referencing a cited web document\nReferences 1, 4 and 6 could not be accessed\n* Publons Reviewer Recognition. Springer Nature can send verification of this review directly to Publons (a subsidiary of Clarivate Analytics). If you would like to take advantage of this service, please click on the “Yes” option below. Your name, email address, title of the reviewed manuscript, name of the journal, and date of your review submission (the “Review Data”) will then be transmitted to Publons upon publication of the manuscript. If you have already registered at Publons, they will notify you of the receipt of this review and update your profile as per your settings and their policy. If you are not registered with Publons, you will receive an email from them asking you to register in order for them to be able to recognize your review on your new profile page. Publons may use the Review Data to generate derivative metadata for the benefit of Publons and you as a reviewer, carefully considering the sensitivity of such information. 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Any comments that I do not wish to be included in the published report have been included as confidential comments to the editor, which will not be published.: **I agree to the terms of the CC-BY 4.0 license; please publish my name with my report.**\n* Is the study design appropriate to answer the research question (including the use of appropriate controls), and are the conclusions supported by the evidence presented?: **Yes**\n* Are the methods sufficiently described to allow the study to be repeated?: **Yes**\n* Is the use of statistics and treatment of uncertainties appropriate?: **Yes**\n* Is the presentation of the work clear?: **Yes**\n* Are the images in this manuscript (including electrophoretic gels and blots) free from apparent manipulation?: **Yes**\n"},{"type":"reviewerAgreed","content":"","date":"2020-08-30T12:00:00+00:00","index":2,"fulltext":""},{"type":"reviewerAgreed","content":"","date":"2020-08-28T12:00:00+00:00","index":1,"fulltext":""},{"type":"reviewersInvited","content":"","date":"2020-08-27T12:00:00+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2020-08-12T12:00:00+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2020-08-12T12:00:00+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2020-08-12T12:00:00+00:00","index":"","fulltext":""},{"type":"submitted","content":"","date":"2020-08-03T12:00:00+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"bmc-pediatrics","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"bped","sideBox":"Learn more about [BMC Pediatrics](http://bmcpediatr.biomedcentral.com/)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/bped/default.aspx","title":"BMC Pediatrics","twitterHandle":"BMC_series","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"em","reportingPortfolio":"BMC Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"f6d3b8e0-b12b-4d67-9d2f-b7bc9faa6f73","owner":[],"postedDate":"November 4th, 2020","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[{"id":980582,"name":"Pediatrics"}],"tags":[],"updatedAt":"2020-11-15T15:03:37+00:00","versionOfRecord":{"articleIdentity":"rs-52787","link":"https://doi.org/10.1186/s12887-020-02412-2","journal":{"identity":"bmc-pediatrics","isVorOnly":false,"title":"BMC Pediatrics"},"publishedOn":"2020-11-14 15:02:09","publishedOnDateReadable":"November 14th, 2020"},"versionCreatedAt":"2020-11-04 19:49:29","video":"","vorDoi":"10.1186/s12887-020-02412-2","vorDoiUrl":"https://doi.org/10.1186/s12887-020-02412-2","workflowStages":[]},"version":"v3","identity":"rs-52787","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-52787","identity":"rs-52787","version":["v3"]},"buildId":"7rjqhiLT3MXkJMwkYKINL","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}