Expression of interleukin-8 in endometriosis eutopic endometrium

Objective:To investigate the role of IL-8 in the eutopic endometriosis.Methods: Immunohistochemistry was used to detect the protein expression of IL-8 in the eutopic endometrium,with the endometrium of nomal fertile women as control.Results: Immunofluorescence: All tissue sections have expression of IL-8 in both gland and stroma,but the expression mainly located in gland.The showed no difference between the(14.743±2.095) and(16.183±3.036)(P0.05).In normal endometrium,expression of IL-8 was significantly higher in proliferative phase(17.204±1.905) than that in secretory phase(26.577±3.202).The expression level of IL-8 in secretory phase(49.122±5.390) was higher than that in proliferative phase(38.506±2.744)(P0.01).Compared with the normal endometrium,the expression level of IL-8 in EMT eutopic endometrium phase was increased(P0.01) in both secretory phase and proliferative phase.Western Blot: In normal endometrium,expression of IL-8 was significantly higher in proliferative phase(0.076±0.015)than that in secretory phase(0.346±0.035).The significant difference existed between secretory phase(0.659±0.043) and proliferative phase(0.437±0.0428) in EMT eutopic endometrium(P0.01).Com-pared with the normal endometrium,the expression level of IL-8 in EMT eutopic endometrium was increased(P0.05,P0.01) in both secretory phase and proliferative phase.Conclusion: IL-8 may play an important role in the pathogenesis of endometriosis.