Investigation of Chikungunya Arbovirus in Cities of Bushehr Province: An ELISA-Based Study: A Cross- Sectional Study

Investigation of Chikungunya Arbovirus in Cities of Bushehr Province: An ELISA-Based Study: A Cross- Sectional Study | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Investigation of Chikungunya Arbovirus in Cities of Bushehr Province: An ELISA-Based Study: A Cross- Sectional Study Ebrahim Abbasi This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7359300/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background: Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that poses a public health threat in regions suitable for Aedes mosquito proliferation. This study aimed to assess the seroprevalence of CHIKV in Bushehr Province, Iran. Methods: A cross-sectional study was conducted from July to September 2023 across ten cities in Bushehr Province. A total of 180 serum samples were collected from blood donors and hospital attendees. CHIKV-specific IgG antibodies were detected using a commercial ELISA kit, and results were interpreted per the manufacturer’s guidelines. Results: Out of 180 samples, 5 tested positive for CHIKV IgG antibodies, indicating a seroprevalence rate of 2.78%. Positive cases were identified in Bushehr, Dashtestan, and Dashti cities, suggesting limited but detectable exposure. Conclusion: Although CHIKV prevalence was low, the presence of seropositive cases indicates potential risk for virus introduction and local transmission. Given the favorable climate for Aedes mosquitoes in the region, continued vector surveillance and preventive public health measures are recommended. Health sciences/Diseases Health sciences/Health care Health sciences/Medical research Biological sciences/Microbiology Chikungunya virus ELISA seroprevalence Aedes mosquitoes Iran public health Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7359300","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":502207075,"identity":"8296def0-a5c0-49ef-8c8d-4946f6e829b9","order_by":0,"name":"Ebrahim Abbasi","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABCklEQVRIie2PMUvEMBTHHxRuCnStBNpPILxSuEWp38K5RyFdInR07OTWXYjgV1CEmyMF3W6OpIP3DSouFQ80OU7BIZXbBPMbwv+F9+PPA/B4/iIRMQ/aABCYENtP+byPkm2V4lcFvhSARWOHKSUU7f3LWB9DSNv1a10vq+vTbm1a8viwcZT0q5ISZHBw9ZjRS9Rnt5qhUcpsLh01iiMF7AAVA+MaRRRWkYulQ0kUz95G/IATxYJ3o1SpqIZJBRWfRwQlYMRmtqVIKJ9uSfsVOyJYkqh/mJmg0xvKa1mg+5ZYt93TuMnjUFwEmmx0kojqbhjO89h5/g7ynXC7idPrP0mafbY9Ho/nP/AJtKpeJRlziCkAAAAASUVORK5CYII=","orcid":"","institution":"Shiraz University of Medical Sciences","correspondingAuthor":true,"prefix":"","firstName":"Ebrahim","middleName":"","lastName":"Abbasi","suffix":""}],"badges":[],"createdAt":"2025-08-12 22:23:07","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-7359300/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-7359300/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":95543303,"identity":"35d1bd63-4c23-4b5c-a573-77e8ae80dfce","added_by":"auto","created_at":"2025-11-10 11:54:14","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":615283,"visible":true,"origin":"","legend":"","description":"","filename":"Manuscriptrevision.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7359300/v1_covered_181dcc9a-132a-47f0-9bc1-c2b6bb2d37dd.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Investigation of Chikungunya Arbovirus in Cities of Bushehr Province: An ELISA-Based Study: A Cross- Sectional Study","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Chikungunya virus, ELISA, seroprevalence, Aedes mosquitoes, Iran, public health","lastPublishedDoi":"10.21203/rs.3.rs-7359300/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7359300/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eBackground:\u003c/strong\u003e Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that poses a public health threat in regions suitable for \u003cem\u003eAedes\u003c/em\u003e mosquito proliferation. This study aimed to assess the seroprevalence of CHIKV in Bushehr Province, Iran.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethods:\u003c/strong\u003e A cross-sectional study was conducted from July to September 2023 across ten cities in Bushehr Province. A total of 180 serum samples were collected from blood donors and hospital attendees. CHIKV-specific IgG antibodies were detected using a commercial ELISA kit, and results were interpreted per the manufacturer’s guidelines.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults:\u003c/strong\u003e Out of 180 samples, 5 tested positive for CHIKV IgG antibodies, indicating a seroprevalence rate of 2.78%. Positive cases were identified in Bushehr, Dashtestan, and Dashti cities, suggesting limited but detectable exposure.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConclusion:\u003c/strong\u003e Although CHIKV prevalence was low, the presence of seropositive cases indicates potential risk for virus introduction and local transmission. Given the favorable climate for \u003cem\u003eAedes\u003c/em\u003emosquitoes in the region, continued vector surveillance and preventive public health measures are recommended.\u003c/p\u003e","manuscriptTitle":"Investigation of Chikungunya Arbovirus in Cities of Bushehr Province: An ELISA-Based Study: A Cross- Sectional Study","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-08-20 08:55:38","doi":"10.21203/rs.3.rs-7359300/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"fa694c16-9c9f-4438-92ce-95368993f398","owner":[],"postedDate":"August 20th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":53341910,"name":"Health sciences/Diseases"},{"id":53341911,"name":"Health sciences/Health care"},{"id":53341912,"name":"Health sciences/Medical research"},{"id":53341913,"name":"Biological sciences/Microbiology"}],"tags":[],"updatedAt":"2025-11-10T11:53:41+00:00","versionOfRecord":[],"versionCreatedAt":"2025-08-20 08:55:38","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-7359300","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7359300","identity":"rs-7359300","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}