Establishment of endometriosis cell model in vitro

Objective To establish a multicellular model of endometriosis in vitro by separating epithelial cell and stomal cell from endometrium.Methods Eutopic endometrium of endometriosis and other benign disease was collected.Primary culture of en- dometrial cells was performed by high concentration collagenase digestion method.Morphology of endometrial cells of two groups was observed under phase-contras microscope.SABC immunocytochemistry to identify the cells,and MTT method to describe the cell growth curve of two groups were done.Results After high concentration collagenase digestion,epithelial and stromal cells of endometriosis and the control group could stick to the wall of culture flask in 48h under microscope,the average life time of epitheli- al cells was 6 weeks;stromal cells was 15 weeks.Immunocytochemical staining against cytokeratin of epithelial cell was positive, and that against vimentin was negative.On the contrary,immunocytochemical staining against vimentin of stromal cell was posi- tive,and that against cytokeratin was negative.The growth curve of two groups was close to each other.Conclusion High con- centration collagenase digestion method to establish multicellular model of endomctriosis in vitro is simple and easy.There is no distinct difference between endometrial cell of endometriosis and normal endometrium in the morphology and growth conditions.It can be used as a model to study gene characteristics of the endometrial cells and other facter's effects on them.