Analysis of γδ~+T cell and natural killed cell cytotoxic activities in peripheral blood and peritoneal fluid of patients with endometriosis

Aim To investigate the roles of γδ+T cell and NK cell activities in the development of endometriosis. Methods The phenotypes and activities of γδ+ T cells and NK cells in peripheral blood and peritoneal fluid of patients with endometriosis were studied by immunofluorescence assay and cytotoxicity assay. Result Percentage of γδ+ T cells in peritoneal fluid of the patients was higher than that in peripheral blood of the patients and in control. The activities of γδ+ T cells and NK cells in peripheral blood and peritoneal fluid of the patients were lower than those in peripheral blood and in control. The peritoneal fluid of the patients could decrease activities of γδ+T cells and NK cells in healthy.Conclusion Activities of γδ+ T cells and NK cells are low state, suggesting that there is suppressive factor directed at these cells in peritoneal fluid of the patients.