Modulation of Cardiac Autonomic Dynamics by Structured Auditory Rhythm: Evidence from ECG and HRV Spectral Analysis

Modulation of Cardiac Autonomic Dynamics by Structured Auditory Rhythm: Evidence from ECG and HRV Spectral Analysis | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Modulation of Cardiac Autonomic Dynamics by Structured Auditory Rhythm: Evidence from ECG and HRV Spectral Analysis Sonali Mohan This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8982875/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Auditory rhythmic stimulation, particularly mantra-based vocalizations, has been increasingly associated with modulation of autonomic nervous system activity and cardiovascular regulation. This study presents a computational and signal-based analysis of neurocardiac responses to 30-minute rhythmic Ram mantra exposure, evaluated using simulated electrocardiographic (ECG) and heart rate variability (HRV) metrics. Frequency-domain HRV analysis demonstrated a significant increase in high-frequency (HF, 0.15–0.40 Hz) spectral power, indicative of enhanced parasympathetic (vagal) activity. Simulated ECG signals revealed increased RR interval duration, reduced heart rate, and improved cardiac rhythm coherence, without pathological waveform alterations. These findings are consistent with vagally mediated autonomic regulation and increased neurocardiac synchronization. Mechanistically, rhythmic auditory stimulation is known to entrain respiratory and neural oscillatory networks via auditory–vagal pathways involving the nucleus tractus solitarius and central autonomic network. Increased HRV and cardiac coherence are established biomarkers of improved cardiovascular adaptability, emotional regulation, and reduced physiological stress load. The present findings align with prior empirical evidence demonstrating that meditation, chanting, and controlled breathing enhance vagal tone and autonomic balance. These results support the hypothesis that structured auditory rhythmic exposure may serve as a non-invasive neuromodulatory intervention capable of improving cardiac autonomic regulation and neurophysiological stability. Cardiac & Cardiovascular Systems Computational Biology Mantra Meditation Computational Biology cardiac autonomic regulation neurophysiological stability Full Text Additional Declarations The authors declare no competing interests. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8982875","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":597862789,"identity":"eb8c1b71-009c-4e4e-9f0d-5123466ab033","order_by":0,"name":"Sonali Mohan","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABBElEQVRIiWNgGAWjYBAC+QYg8cCAgQGIGB9+qLABchkbD+DTYgCSTYBoYTaWOJMG0tKAXwuISIAw2CR4Ww6DRfFrYe89+CGhYJucOfvZAxKSDeft1rYfBtpSYxON0y8955IlEgxuG1v25CUYFO64nbztTCJQy7G03AZcem7kGIC0JG44kGOQIHnmdrLZAaAWxobD+LQY/wBrOf/G4ABv27lks/MPCWoxg9hyI8ewgbftgJ3ZDQK2GJw5Y2YB8ovBjTfGzBJnkhPMbgBtScDjF/n2HuMbH/7cljM4n2P+80OFnb3Z+fSHDz7U2OB2GDpIBKtMIFY5CNiTongUjIJRMApGBgAASqhr9O6LJ6oAAAAASUVORK5CYII=","orcid":"","institution":"INDEPENDENT RESEARCHER","correspondingAuthor":true,"prefix":"","firstName":"Sonali","middleName":"","lastName":"Mohan","suffix":""}],"badges":[],"createdAt":"2026-02-27 03:11:08","currentVersionCode":1,"declarations":{"humanSubjects":false,"vertebrateSubjects":false,"conflictsOfInterestStatement":false,"humanSubjectEthicalGuidelines":false,"humanSubjectConsent":false,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":false},"doi":"10.21203/rs.3.rs-8982875/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-8982875/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":103745975,"identity":"1e34e301-cdb1-4ead-aefd-c27c6294ec93","added_by":"auto","created_at":"2026-03-02 12:03:39","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":361330,"visible":true,"origin":"","legend":"","description":"","filename":"2026CPNaturalMedicine24ModulationofCardiacAutonomicDynamicsbyStructuredAuditoryRhythmEvidencefromECGandHRVSpectralAnalysis.pdf","url":"https://assets-eu.researchsquare.com/files/rs-8982875/v1_covered_74f00e69-2d29-45be-a0db-1ebdcb70f332.pdf"}],"financialInterests":"The authors declare no competing interests.","formattedTitle":"\u003cp\u003e\u003cstrong\u003eModulation of Cardiac Autonomic Dynamics by Structured Auditory Rhythm: Evidence from ECG and HRV Spectral Analysis\u003c/strong\u003e\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Mantra Meditation, Computational Biology, cardiac autonomic regulation, neurophysiological stability","lastPublishedDoi":"10.21203/rs.3.rs-8982875/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-8982875/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eAuditory rhythmic stimulation, particularly mantra-based vocalizations, has been increasingly associated with modulation of autonomic nervous system activity and cardiovascular regulation. 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