Expressions of growth differentiation factor-9 (gdf-9) of bovine cumulus-oocyte complex in peritoneal fluid culture of infertile patients with endometriosis

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Abstract

The decrease of GDF-9 production may impair oocyte granulosa cell communication and lead to failure of fertilization and embryonic development. The objective of this study was to study expression of growth differentiation factor-9 (GDF-9) cumulus-oocyte complex in peritoneal culture of infertile patients with endometriosis. Peritoneal fluid were collected from infertile patients undergoing diagnostic laparoscopy and classified into non-endometriosis, mild endometriosis, and severe endometriosis according to laparoscopic finding. Cumulus-oocyte complex (COC) were aspirated from antral follicle derived from bovine ovary from local abattoirs. COC were then placed in tissue culture medium-199 (TCM-199) and divided into four group of cultere. First group contain TCM-199 as a medium served as control group, while second, third, and four groups medium were added accordingly with 3% of peritoneal fluid of non endometriosis, mild endometriosis, and severe endometriosis. These four groups were incubated in 5% CO2 at 38oC for 20 hours and COC were eventually fixated in object glasses, stained by immunocytochemistry, and followed by measurement of GDF-9 expression. Statistic analysis using Kruskal Wallis test showed differences of GDF-9 expression in four culture groups. Mann-Whitney test revealed that GDF-9 expression in endometriosis group (mild and severe) were significantly lower compared with control and non-endometriosis group and expression in severe endometriosis group were significantly lower than in mild endometriosis one (p < 0.05). In conclusion,GDF-9 expressions of bovine COC cultured in peritoneal fluid of infertile patients with endometriosis were lowered compared with that of control and non-endometriosis group and this decrease was in proportion with severity of endometriosis.(MOG 2013;21:71-76)
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Expressions of Growth Differentation Factor-9 (GDF-9) of Bovine Cumulus-Oocyte Complex in Peritoneal Fluid Culuture of Infertile Patients with Endometriosis) by Hendy Hendarto Submission date: 01-Mar-2019 11:56AM (UTC+0800) Submission ID: 1085782530 File name: 25._Expression_of_Growth.pdf (230.12K) Word count: 3337 Character count: 19604 25 % SIMILARITY INDEX 17 % INTERNET SOURCES 23 % PUBLICATIONS 0 % STUDENT PAPERS 1 1 % 2 1 % 3 1 % 4 1 % 5 1 % 6 1 % Expressions of Growth Differentation Factor-9 (GDF-9) of Bovine Cumulus-Oocyte Complex in Peritoneal Fluid Culuture of Infertile Patients with Endometriosis) ORIGINALITY REPORT PRIMARY SOURCES theses.ulaval.ca Internet Source Harada, T.. "Role of cytokines in endometriosis", Fertility and Sterility, 200107 Publication M. Carlberg. "Elevated expression of tumour necrosis factor alpha in cultured granulosa cells from women with endometriosis", Human Reproduction, 06/01/2000 Publication Ming-Yih Wu. "The Role of Cytokines in Endometriosis*", American Journal Of Reproductive Immunology, 5/2003 Publication jcs.biologists.org Internet Source E Oral. "The peritoneal environment in endometriosis", Human Reproduction Update, 7 1 % 8 1 % 9 1 % 10 1 % 11 1 % 12 1 % 13 1 % 1996 Publication Osmond J. D?Cruz. "Targeting Mast Cells in Endometriosis with Janus Kinase 3 Inhibitor, JANEX-1", American Journal Of Reproductive Immunology, 8/2007 Publication pdfpills.com Internet Source www.penelitian.unair.ac.id Internet Source J. Eppig. "Oocyte control of ovarian follicular development and function in mammals", Reproduction, 12/01/2001 Publication Gihan Mansour, Rakesh K. Sharma, Ashok Agarwal, Tommaso Falcone. "Endometriosis- induced alterations in mouse metaphase II oocyte microtubules and chromosomal alignment: a possible cause of infertility", Fertility and Sterility, 2010 Publication www.touchobgyn.com Internet Source Stokes, P.J.. "Development of porcine embryos in vivo and in vitro; evidence for embryo 'cross 14 1 % 15 1 % 16 1 % 17 1 % 18 1 % 19 1 % 20 1 % 21 < 1 % talk' in vitro", Developmental Biology, 20050801 Publication spandidos-publications.com Internet Source Chang, H.-M., J.-C. Cheng, E. Taylor, and P. C. K. Leung. "Oocyte-derived BMP15 but not GDF9 down-regulates connexin43 expression and decreases gap junction intercellular communication activity in immortalized human granulosa cells", Molecular Human Reproduction, 2014. Publication www.scribd.com Internet Source www.inviafertility.com Internet Source journal.frontiersin.org Internet Source penelitian.unair.ac.id Internet Source "Endometriosis and infertility: a committee opinion", Fertility and Sterility, 2012 Publication www.endometriosi.it Internet Source 22 < 1 % 23 < 1 % 24 < 1 % 25 < 1 % 26 < 1 % 27 < 1 % 28 < 1 % Geetu Pahlajani. "Laparoscopic surgery for endometriosis-associated infertility: a pathophysiologic approach", Gynecological Surgery, 03/09/2010 Publication www.biomedcentral.com Internet Source Julia A. Elvin, Amander T. Clark, Pei Wang, Neil M. Wolfman, Martin M. Matzuk. "Paracrine Actions Of Growth Differentiation Factor-9 in the Mammalian Ovary", Molecular Endocrinology, 1999 Publication etheses.whiterose.ac.uk Internet Source Shekhar, Shashank, Chanderdeep Sharma, and Kamal Singh. "Spontaneous viable birth in a case of stage III pelvic endometriosis with bilateral tubal damage following diagnostic intervention: who deserves the credit?", Archives of Gynecology and Obstetrics, 2013. Publication unsri.portalgaruda.org Internet Source Julie A. W. Stilley. "Cellular and molecular basis for endometriosis-associated infertility", 29 < 1 % 30 < 1 % 31 < 1 % 32 < 1 % 33 < 1 % 34 < 1 % Cell & Tissue Research, 02/03/2012 Publication Qing-Qing Shi, Min Sun, Zhen-Tao Zhang, Ya- Ni Zhang, Ahmed Kamel Elsayed, Lei Zhang, Xiao-Mei Huang, Bi-Chun Li. "A screen of suitable inducers for germline differentiation of chicken embryonic stem cells", Animal Reproduction Science, 2014 Publication Cohen, J., A. Ziyyat, I. Naoura, N. Chabbert- Buffet, S. Aractingi, E. Darai, and B. Lefevre. "Effect of induced peritoneal endometriosis on oocyte and embryo quality in a mouse model", Journal of Assisted Reproduction and Genetics, 2015. Publication Fang Lian. "Effect of Quyu Jiedu Granule ( 祛瘀 解毒 颗 粒 ) on microenvironment of ova in patients with endometriosis", Chinese Journal of Integrative Medicine, 02/2009 Publication e-journal.unair.ac.id Internet Source "Abstracts", Theriogenology, 20030101 Publication S Martinez-Roman. "Immunological factors in endometriosis-associated reproductive failure: 35 < 1 % 36 < 1 % 37 < 1 % 38 < 1 % 39 < 1 % 40 < 1 % 41 studies in fertile and infertile women with and without endometriosis", Human Reproduction, 08/01/1997 Publication T. Harada. "Apoptosis in human endometrium and endometriosis", Human Reproduction Update, 01/01/2004 Publication Kokcu, Arif. "Possible effects of endometriosis- related immune events on reproductive function", Archives of Gynecology and Obstetrics, 2013. Publication Richards, JoAnne S., Darryl L. Russell, Scott Ochsner, and Lawrence L. Espey. "Ovulation: New Dimensions and New Regulators of the Inflammatory-Like Response", Annual Review of Physiology, 2002. Publication www.bioscience.org Internet Source www.endometriosis.ca Internet Source ijfs.ir Internet Source Yulia Antsiferova, Natalya Sotnikov. "Chapter 3 < 1 % 42 < 1 % 43 < 1 % 44 < 1 % 45 < 1 % 46 < 1 % The Local Immune Mechanisms Involved in the Formation of Endometriotic Lesions", InTech, 2012 Publication L. Vanhoutte. "Prematuration of human denuded oocytes in a three-dimensional co- culture system: effects on meiosis progression and developmental competence", Human Reproduction, 12/04/2008 Publication www.vitals.com Internet Source S. J. Tanahatoe. "Investigation of the infertile couple: Should diagnostic laparoscopy be performed in the infertility work up programme in patients undergoing intrauterine insemination?", Human Reproduction, 01/01/2003 Publication Yuri Takemura. "Concentration of Adiponectin in Peritoneal Fluid is Decreased in Women with Endometriosis", American Journal Of Reproductive Immunology, 10/2005 Publication ccf.org Internet Source www.journal.unair.ac.id 47 < 1 % 48 < 1 % 49 < 1 % 50 < 1 % 51 < 1 % 52 < 1 % 53 < 1 % 54 < 1 % Internet Source health.usnews.com Internet Source Lin, Z-L, Y-H Li, Y-N Xu, Q-L Wang, S Namgoong, X-S Cui, and N-H Kim. "Effects of Growth Differentiation Factor 9 and Bone Morphogenetic Protein 15 on the in vitro Maturation of Porcine Oocytes", Reproduction in Domestic Animals, 2013. Publication www.doria.fi Internet Source zombiedoc.com Internet Source H.-F. Huang. "High bone morphogenetic protein-15 level in follicular fluid is associated with high quality oocyte and subsequent embryonic development", Human Reproduction, 03/21/2007 Publication "Abstracts for IPNA Congress in Shanghai, China 2013", Pediatric Nephrology, 2013. Publication Chuderland, D., N. Hasky, I. Ben-Ami, R. Kaplan-Kraicer, H. Grossman, and R. Shalgi. "A 55 < 1 % Exclude quotes On Exclude bibliography On Exclude matches Off physiological approach for treating endometriosis by recombinant pigment epithelium-derived factor (PEDF)", Human Reproduction, 2013. Publication Bersinger, N.A.. "PAPP-A and osteoprotegerin, together with interleukin-8 and RANTES, are elevated in the peritoneal fluid of women with endometriosis", American Journal of Obstetrics and Gynecology, 200607 Publication FINAL GRADE /100 Expressions of Growth Differentation Factor-9 (GDF-9) of Bovine Cumulus-Oocyte Complex in Peritoneal Fluid Culuture of Infertile Patients with Endometriosis) GRADEMARK REPORT GENERAL COMMENTS Instructor PAGE 1 PAGE 2 PAGE 3 PAGE 4 PAGE 5 PAGE 6

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