Lateral Motor Column specific expression of Sonic Hedgehog contributes to maintenance and scaling of pMN progenitor cell populations during oligodendrogenesis

Lateral Motor Column specific expression of Sonic Hedgehog contributes to maintenance and scaling of pMN progenitor cell populations during oligodendrogenesis | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Lateral Motor Column specific expression of Sonic Hedgehog contributes to maintenance and scaling of pMN progenitor cell populations during oligodendrogenesis Andreas Kottmann, Lev Starikov, Miruna Ghinia-Tegla This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4249282/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted You are reading this latest preprint version Abstract Motor neurons (MNs) and oligodendrocyte precursor cells (OPCs) emerge sequentially from the pMN precursor domain during spinal cord development. MNs diversify into muscle specific subtypes and settle in stereotypic locations in the ventral horns. In contrast, OPCs are mobile and appear to evenly populate the parenchyma. Whether earlier born MNs influence OPC production is controversial. We found that Sonic Hedgehog signaling emanating from nascent MNs of the lateral motor column is critical for maintaining a larger and more yielding pMN domain at limb levels compared to trunk levels during OPC production. Reduced Shh signaling resulted in unrecoverable diminishment of pMN domain based OPC production leaving the spinal cord impoverished of OPC. Our results suggest that production of OPC at limb levels is contingent on completion of MN production. Biological sciences/Developmental biology/Morphogenesis/Body patterning Biological sciences/Neuroscience/Gliogenesis Health sciences/Neurology/Neurological disorders/Multiple sclerosis Sonic hedgehog signaling motor neurons pMN domain oligodendrocyte precursor spinal cord sculpting. Full Text Additional Declarations There is NO Competing Interest. Supplementary Files nrreportingsummaryforShhscalesOPCnumbersStarikovetal.pdf Reporting Summary Cite Share Download PDF Status: Under Review Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4249282","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":292809941,"identity":"68076be7-2ed9-4eb3-b601-5e43032b2fb9","order_by":0,"name":"Andreas Kottmann","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA7klEQVRIiWNgGAWjYDACHhiDvQGJw4NVLVySseEAmHGAZC0SCURq4e85fPzxxxy7PHPJ14kP3jDYJW6XSGB88LYNtxaJs22JDQe3JRdbzs7dbDiHITlx54wEZsO5eLQwnOcxBGphTtxwO3ebNA/DgcQNNxLYpHnxaJGHaKlP3HDz7PbfUC3sv/FpMTjbA9JyGKiSdxszzBZmfFoMzxxLnHF22/HEDWdyN0vOMUg23nDmYbPknHO4tcidST7woXJbdeKG42c3fnhTYSe74XjywQ9vyvB4H82dQCyQ2EC0eijgP0CqjlEwCkbBKBjmAACu511mb0GTLQAAAABJRU5ErkJggg==","orcid":"https://orcid.org/0000-0002-5435-7502","institution":"City University of New York School of Medicine (CSOM), Dept. of Molecular, Cellular and Biomedical Sciences, New York City, NY 10031, USA","correspondingAuthor":true,"prefix":"","firstName":"Andreas","middleName":"","lastName":"Kottmann","suffix":""},{"id":292809942,"identity":"7b9946fb-6d72-46a0-99e1-93e2394cdf1d","order_by":1,"name":"Lev Starikov","email":"","orcid":"","institution":"City University of New York School of Medicine (CSOM), Dept. of Molecular, Cellular and Biomedical Sciences, New York City, NY 10031, USA","correspondingAuthor":false,"prefix":"","firstName":"Lev","middleName":"","lastName":"Starikov","suffix":""},{"id":292809943,"identity":"f9cd66b9-991a-4089-afea-38707ade7f27","order_by":2,"name":"Miruna Ghinia-Tegla","email":"","orcid":"","institution":"City University of New York School of Medicine (CSOM), Dept. of Molecular, Cellular and Biomedical Sciences, New York City, NY 10031, USA","correspondingAuthor":false,"prefix":"","firstName":"Miruna","middleName":"","lastName":"Ghinia-Tegla","suffix":""}],"badges":[],"createdAt":"2024-04-10 21:35:08","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4249282/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4249282/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":56349046,"identity":"f734e22d-91cc-4176-bcb8-88e23d2fd387","added_by":"auto","created_at":"2024-05-13 03:21:26","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":3093533,"visible":true,"origin":"","legend":"","description":"","filename":"ShhscalesspinalOPCnumbersStarikovetalCB.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4249282/v1_covered_811c11e5-0eb5-4dec-9433-792e24605a7d.pdf"},{"id":56348655,"identity":"b36075d2-2433-42f3-824a-77f42c804ae1","added_by":"auto","created_at":"2024-05-13 03:13:22","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":1666182,"visible":true,"origin":"","legend":"Reporting Summary","description":"","filename":"nrreportingsummaryforShhscalesOPCnumbersStarikovetal.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4249282/v1/7e492cc401a6b2344fb3a4db.pdf"}],"financialInterests":"There is \u003cb\u003eNO\u003c/b\u003e Competing Interest.","formattedTitle":"Lateral Motor Column specific expression of Sonic Hedgehog contributes to maintenance and scaling of pMN progenitor cell populations during oligodendrogenesis","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"nature-portfolio","isNatureJournal":true,"hasQc":false,"allowDirectSubmit":false,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"","title":"Nature Portfolio","twitterHandle":"","acdcEnabled":false,"dfaEnabled":false,"editorialSystem":"ejp","reportingPortfolio":"","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"Sonic hedgehog signaling, motor neurons, pMN domain, oligodendrocyte precursor, spinal cord sculpting.","lastPublishedDoi":"10.21203/rs.3.rs-4249282/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4249282/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Motor neurons (MNs) and oligodendrocyte precursor cells (OPCs) emerge sequentially from the pMN precursor domain during spinal cord development. 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