Maternal Environment Alters DNA Methylation Inheritance in Chinook Salmon (Oncorhynchus tshawytscha): Maternal Effects on Gene-Specific DNA Methylation

Maternal Environment Alters DNA Methylation Inheritance in Chinook Salmon (Oncorhynchus tshawytscha): Maternal Effects on Gene-Specific DNA Methylation | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 8 March 2025 V1 Latest version Share on Maternal Environment Alters DNA Methylation Inheritance in Chinook Salmon (Oncorhynchus tshawytscha): Maternal Effects on Gene-Specific DNA Methylation Authors : James Watkins 0009-0003-0319-4461 [email protected] , Clare Venney , Matthew Yates 0000-0002-9199-1078 , and Daniel Heath 0000-0001-5762-3653 Authors Info & Affiliations https://doi.org/10.22541/au.174141934.47421407/v1 355 views 156 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Maternal effects are a prevalent source of early life phenotypic variation in offspring across diverse taxa and have been shown to provide offspring an adaptive advantage in response to maternal environmental stimuli. There are several well studied examples of adaptive maternally induced intergenerational effects in response to the parental environment mediated by mechanisms such as stress hormones and nutrients. DNA methylation is a fundamental cellular process that affects gene transcription and can respond rapidly to changing environments yet remains a largely unexplored mechanism for maternal effect signaling. We manipulated maternal environment in sexually maturing female Chinook salmon (Oncorhynchus tshawytscha) by reducing food availability or increasing day length. We used a factorial breeding design as well as gene-specific sequencing assays to analyze how maternal effects influenced DNA methylation in offspring at the eyed egg and alevin developmental stages We found significant maternal effects on gene-specific DNA methylation as well as heightened levels of maternal effects in response to an increase in maternal photoperiod. We report higher maternal effects in early life stages that decline through development in four gene functional categories: growth, immune response, metabolic function, and histone protein regulation. Despite a potential resetting of the methylome following fertilization, we provide evidence that maternal effects can modulate gene-specific DNA methylation, and that effect is sensitive to the environment the mothers experience. This pattern of epigenetically-mediated maternal effects responding to maternal environment is consistent with a fundamental process driving intergenerational phenotypic variation. Supplementary Material File (jamesmanuscript_final_v2.docx) Download 492.43 KB Information & Authors Information Version history V1 Version 1 08 March 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords aquaculture development and evolution fish molecular evolution quantitative genetics Authors Affiliations James Watkins 0009-0003-0319-4461 [email protected] University of Windsor View all articles by this author Clare Venney University of Alberta View all articles by this author Matthew Yates 0000-0002-9199-1078 University of Windsor View all articles by this author Daniel Heath 0000-0001-5762-3653 University of Windsor View all articles by this author Metrics & Citations Metrics Article Usage 355 views 156 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation James Watkins, Clare Venney, Matthew Yates, et al. Maternal Environment Alters DNA Methylation Inheritance in Chinook Salmon (Oncorhynchus tshawytscha): Maternal Effects on Gene-Specific DNA Methylation. Authorea . 08 March 2025. DOI: https://doi.org/10.22541/au.174141934.47421407/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); View Options View options PDF View PDF Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. 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