Biocontrol Potential of Kosakonia cowanii Strain TKS G-1 against Wheat Stripe Rust caused by Puccinia striiformis f. sp. tritici

Biocontrol Potential of Kosakonia cowanii Strain TKS G-1 against Wheat Stripe Rust caused by Puccinia striiformis f. sp. tritici | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Biocontrol Potential of Kosakonia cowanii Strain TKS G-1 against Wheat Stripe Rust caused by Puccinia striiformis f. sp. tritici Qiqi Zhang, Niannian Yan, Qi Liu, Jing Chen, Jianaerguli Nusifuaili This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8755098/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 6 You are reading this latest preprint version Abstract Background Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici ( Pst ), poses a significant threat to global wheat production. Results This study aimed to evaluate the biocontrol potential of endophytic bacteria against this devastating disease. Strain TKS G-1, isolated from healthy wheat roots, was identified as Kosakonia cowanii through polyphasic taxonomy (morphological, physiological, and phylogenetic analysis of 16S rDNA and rpo B sequences). The (fermented liquid with bacterial cells) FLBC of TKS G-1 inhibited Pst urediospore germination by 88.89% in vitro. In pot tests, treatment with FLBC of TKS G-1 achieved 64.95% disease control efficacy, while significantly promoting plant height, fresh weight, and dry weight compared to the pathogen-only control. Further analyses revealed that the antifungal activity was primarily mediated by extracellular secondary metabolites distinct from volatile organic compounds. Additionally, strain TKS G-1 exhibited multiple plant growth-promoting (PGP) traits, including indole-3-acetic acid (IAA) production, siderophore secretion, amylase and ACC deaminase activity, inorganic phosphate solubilization, and atmospheric nitrogen fixation. Conclusions These results collectively demonstrated that Kosakonia cowanii TKS G-1 was a promising candidate for integrated management of wheat stripe rust, functioning through both direct antagonism and induced systemic resistance coupled with growth promotion. Wheat stripe rust biocontrol agent Kosakonia cowanii efficacy evaluation Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Under Review Version 1 posted Reviews received at journal 16 Mar, 2026 Reviewers agreed at journal 02 Mar, 2026 Reviewers invited by journal 01 Mar, 2026 Editor assigned by journal 03 Feb, 2026 Submission checks completed at journal 03 Feb, 2026 First submitted to journal 01 Feb, 2026 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8755098","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":599148814,"identity":"75317fca-fc48-412e-bf55-6dc96a6482ce","order_by":0,"name":"Qiqi Zhang","email":"","orcid":"","institution":"Xinjiang Agricultural University","correspondingAuthor":false,"prefix":"","firstName":"Qiqi","middleName":"","lastName":"Zhang","suffix":""},{"id":599148817,"identity":"60fe19fd-9e6c-4e82-9ea0-173324affde4","order_by":1,"name":"Niannian Yan","email":"","orcid":"","institution":"Xinjiang Agricultural 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Agriculture","twitterHandle":"@SpringerPlants","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"BMC/SO AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Wheat stripe rust, biocontrol agent, Kosakonia cowanii, efficacy evaluation","lastPublishedDoi":"10.21203/rs.3.rs-8755098/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-8755098/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eBackground \u003c/strong\u003eWheat stripe rust, caused by \u003cem\u003ePuccinia striiformis\u003c/em\u003e f. sp. \u003cem\u003etritici\u003c/em\u003e(\u003cem\u003ePst\u003c/em\u003e), poses a significant threat to global wheat production.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults \u003c/strong\u003eThis study aimed to evaluate the biocontrol potential of endophytic bacteria against this devastating disease. Strain TKS G-1, isolated from healthy wheat roots, was identified as \u003cem\u003eKosakonia cowanii\u003c/em\u003e through polyphasic taxonomy (morphological, physiological, and phylogenetic analysis of 16S rDNA and \u003cem\u003erpo\u003c/em\u003eB sequences). The (fermented liquid with bacterial cells) FLBC of TKS G-1 inhibited\u003cem\u003e Pst\u003c/em\u003e urediospore germination by 88.89% in vitro. In pot tests, treatment with FLBC of TKS G-1 achieved 64.95% disease control efficacy, while significantly promoting plant height, fresh weight, and dry weight compared to the pathogen-only control. Further analyses revealed that the antifungal activity was primarily mediated by extracellular secondary metabolites distinct from volatile organic compounds. Additionally, strain TKS G-1 exhibited multiple plant growth-promoting (PGP) traits, including indole-3-acetic acid (IAA) production, siderophore secretion, amylase and ACC deaminase activity, inorganic phosphate solubilization, and atmospheric nitrogen fixation.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConclusions \u003c/strong\u003eThese results collectively demonstrated that \u003cem\u003eKosakonia cowanii \u003c/em\u003eTKS G-1 was a promising candidate for integrated management of wheat stripe rust, functioning through both direct antagonism and induced systemic resistance coupled with growth promotion.\u003c/p\u003e","manuscriptTitle":"Biocontrol Potential of Kosakonia cowanii Strain TKS G-1 against Wheat Stripe Rust caused by Puccinia striiformis f. sp. tritici","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2026-03-04 10:21:21","doi":"10.21203/rs.3.rs-8755098/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"editorInvitedReview","content":"","date":"2026-03-16T21:06:16+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"96502434484652356157131669991408755941","date":"2026-03-02T08:54:59+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2026-03-02T04:26:30+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2026-02-03T18:00:39+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2026-02-03T18:00:27+00:00","index":"","fulltext":""},{"type":"submitted","content":"Chemical and Biological Technologies in Agriculture","date":"2026-02-01T09:11:23+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"chemical-and-biological-technologies-in-agriculture","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"","sideBox":"Learn more about [Chemical and Biological Technologies in Agriculture](https://chembioagro.springeropen.com/)","snPcode":"40538","submissionUrl":"https://submission.nature.com/new-submission/40538/3","title":"Chemical and Biological Technologies in Agriculture","twitterHandle":"@SpringerPlants","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"BMC/SO AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"b96a4b1c-bdeb-4517-b588-c2e4079dd146","owner":[],"postedDate":"March 4th, 2026","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"under-review","subjectAreas":[],"tags":[],"updatedAt":"2026-03-04T10:21:22+00:00","versionOfRecord":[],"versionCreatedAt":"2026-03-04 10:21:21","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-8755098","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-8755098","identity":"rs-8755098","version":["v1"]},"buildId":"XKTyCvWXoU3ODBz1xrDgd","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}